본 연구에서는 붉은장목수수(Sorglum Bicolor M.) MeOH 추출물과 분획물들의 다양한 생리활성을 비교하기 위하여 항산화(DPPH assay, 환원력, 총 페놀 및 플라보노이드 함량), 항당뇨(α-Glucosidase 저해활성 및 α-Amlyase 저해활성), 항암(MTT assay) 활성을 관찰 하였다. 항산화활성의 경우 EtOAc fraction이 높은 총 페놀과 플라보노이드 함량을 나타내었으며, DPPH assay와 reducing power 결과 역시 EtOAc fraction과 MeOH fraction이 positive control로 사용한 α-tocopherol보다 우수한 활성을 나타냈다. α-Glucosidase, α-amlyase 저해능 평가 결과 D.W. fraction이 가장 높은 활성을 보였으며 n-BuOH fraction과 MeOH extract도 활성을 나타내었다. 암세포인 AGS, HT29, HCT116세포주에 대한 세포독성 연구인 MTT assay에서는 EtOAc, n-BuOH, D.W. fraction을 처리한 처리구에서 독성을 보였으며 이중 n-BuOH fraction이 모든 세포주에서 농도 의존적으로 세포독성을 가지는 것을 확인할 수 있었다. 연구결과 붉은장목수수의 추출물 EtOAc fraction의 항산화활성, D.W. fraction의 항당뇨활성, n-BuOH fraction의 암세포에 대한 독성과 관련된 화합물의 분리 및 구조규명에 관한 연구가 필요 할 것으로 사료되어지며, normal cell에 대한 MTT assay를 진행하여 항암활성에 관한 연구를 진행하여 붉은장목수수 추출물을 이용한 다양한 건강기능식품과 의약품 개발을 통해 건강 증진과 질병으로부터 보호 받을 수 있을 것으로 기대한다.

This study was carried out to select new pelleting binder and material for seeds from Fraxinus rhynchophylla Hance and Alnus sibirica Fisch. ex Turcz. The optimum treatments of the various concentrations and species of priming agents to improve seed germination of both woody medicinal plants were also estimated. Germinability was increased when the seeds of Fraxinus rhynchophylla Hance was soaked in -1.0 MPa of PEG6000 solution at 15℃ for 4 days significantly, the optimum treatment for improving germination of Alnus sibirica Fisch. ex Turcz was observed when the tested seeds was soaked in 100 mM of KCl at 15℃ for 4 days. The influence of physical and chemical properties of pelleting solid materials, the mixture of gypsum, diatomaceous earth, dalma ceramic and vermicuolite (6:1:1:1 ratio) were found as the best pelleting materials for Fraxinus rhynchophylla Hance and Alnus sibirica Fisch. ex Turcz. seeds. To satisfy the requirements of absorption and compatibility for multi-layer seed pelleting, SGPA (Starch-grafted cross-linked polyacrylates) hydrogel was prepared using starch, acrylonitrile, ceric ammonium nitrate, nitric acid, methyl alcohol and potassium hydroxide. The resulting SGPA hydrogel showed high water absorption but not plant compatibility. It suggested that seed pelleting using pelleting materials and SGPA hydrogel (multi-layer coating) after priming agent treatment is to increase germinability and seedling growth and it can reduced irrigation labours and can also save seed.

This study was carried out to select the appropriate medium (especially, carbon and nitrogen source, potassium phosphate and pH) for somatic embryogenesis in order to develop the rapid mass production system in suspension culture of Oplopanax elatus Nakai. Direct somatic embryos were obtained from root explants in the hormone free suspension culture (MS). Combination of NH4NO3 and KNO3 at the ratio of 1650 (mg/l) : 1900 (mg/l) obtained the better result to produce somatic embryo in suspension culture. MS medium supplemented with 170mg/l KH2PO4. The addition of 1 and 3% sucrose was effective for formation of embryogenic callus. Therefore, this report will be helped to improve the establishment for suspension culture in Oplopanax elatus Nakai.

This study was conducted to determine the contents of phenolic compounds and astragalosides in Astragalus membranaceus. Between the phenolic compound groups, flavonoids compounds (51.13%) had higher average concentrations than the phenolic acids groups. Among the 30 phenolic compounds, quercetin (353.11μg g-1) provided the highest concentrations. According to different cultivation year, 3-year-cultivated Astragalus (2612.57μg g-1) showed the highest concentrations of phenolic compounds. According to different harvest days, 6/5 harvesting Astragalus (2615.80μg g-1) showed the highest concentrations of phenolic acids. Comparison between the top and subterranean parts of harvested Astragalus plants cultivated for 2 years, 3 years, and 5 years showed big difference of total phenolic compounds in concentrations. Further, the top part had higher amounts of the total phenolic compounds than the subterranean part among all Astragalus. This tendency was similar to those of the top root and lateral root. The concentration of the phenolic compounds in the top root was higher than that of the lateral root.

This study was investigated seed germination of Rheum undulatum L. depends on storage temperature (-20, 5℃ and room temperature), germination temperature (5, 15, 25 and 40℃) and a soaking treatment (no soaking, 2 days and 4 days). There was not much difference from the germination rate of R. undulatum L. in storage temperature conditions for 8 weeks. The highest of germination rate occurred over 80 percentage at 25℃. When R. undulatum L. was soaked with tap water for four days, the average values of germination velocity and germination day were represented of 1.7 ea/day and 1.68 days. These results may help our understanding of variations in germination characteristics for R. undulatum L. seeds treated under various germination conditions.

억새 수집종의 초장 길이를 조사한 결과, 개화 전과 후 전체 생육기간 동안 200 cm 이상 우량한 생육을 나타낸 개체 자원은 India에서 수집한 억새 MS013이며, 개화 후에는 MS005, MS010, MS011, MS014 억새도 200 cm 이상의 생육을 보여주었다. 대부분의 억새 수집종의 경우 분얼수가 2-3개 정도로 나타났다. 엽장의 길이가 100 cm 이상인 수집종은 MS001, MS002, MS005, MS012, MS013, MS014로 6종이며, 엽폭은 1-2 cm 사이가 63%이상으로 대부분의 수집종들이 이에 속하였으며, MS002, MS003, MS005 억새의 엽폭은 2 cm 이상으로 잎이 넓은 것을 확인할 수 있었다. MS005 억새는 초장, 간경, 마디수, 분얼수 뿐만 아니라, 엽장, 엽폭에 있어서도 다른 지역에서 수집한 억새보다 수치가 높은 것을 확인하였으며 바이오에너지용 억새로서의 가치가 있다고 사료된다.

This study is part of research to develop the technology for managing major medicinal crops after harvest. We studied the optimal germination conditions of Cynanchum wilfordii (Maxim.) Hemsl. The mean germination time (MGT) of C. wilfordii seeds was higher after soaking for 4 days after storage at 5℃ for 8 weeks, than with germination at 25℃ (3 days). However, the germinative energy (GE) decreased as the number of days soaking increased. The greatest germination rate (83.3±8.8%) was at 25℃ with no soaking of seeds stored at -20℃ for 8 weeks. Based on these results, we characterized the germination conditions of a major medicinal crop.

This study evaluated the germination rate of Rheum palmatum L. in Korea as affected by storage temperature, germination temperature, and a soaking treatment. The germination rate of R. palmatum L. stored at various conditions for 8 weeks was 〉 60%. The highest germination rate occurred at 25℃. The germination point of R. palmatum L. with soaking was started 1 day after application, and non soaked seeds germinated in 2 days. When R. palmatum L. was soaked with water, the germination rate was lower, but mean germination velocity was the highest. These results may help our understanding of variations in germination characteristics for seeds treated under different germination conditions.

본 연구는 미국 수수 수집종의 작물학적 특성 및 생리활성을 검정하기 위하여 수행되었다. 대부분의 미국 수수 수집종은 200 cm 이상의 큰 초장을 보였으며, 간경 또한 2cm 이상의 값을 나타냈다. 수수 수집종 중에서는 Sweet-N-Sterile(#4)이 가장 우량한 수수의 생장 특성을 보였다. 수수의 DPPH를 이용한 활성 검정 결과, Premium stock(#1), Early Sumac(#7), SS Silage(#9), WGF Grain Sorghum(#11) 4개의 수집종에서 기존의 항산화제인 α-tocopherol과 BHA와 비슷한 활성을 보였고 BHT보다는 높은 활성을 보였다. 항미생물 실험결과 BMR Gold I(#3)이 가장 높은 활성을 나타냈으며, 특히 Escherichia coli에서 125 μg/ml으로 높은 활성수치를 나타냈다.

Morus alba (Mulberry) leaves were exposed to fermentation for varying amounts of time: no fermentation (0 min, MANF), medial fermentation (10 h, MAMF), and full fermentation (24 h, MAFF). The chemical compositions of the teas were determined and compared with those of commercial Camellia sinensis teas. The results showed that mulberry leaf teas contained significantly higher amounts of ash and fat than Camellia sinensis tea. Compared with Camellia sinensis teas, all mulberry leaf teas contained significantly more total free amino acids (24.26~54.25mg L-glutamic acid equivalent g-1), but the concentration of caffeine was relatively low for mulberry leaf teas. High thiamine, riboflavin, and niacin contents were found in all mulberry leaf teas, but ascorbic acid and pyridoxine were found at higher levels in Camellia sinensis teas than in mulberry leaf teas. Color measurements demonstrated that mulberry leaf tea infusions generally had lower a* (greenness) and b* (yellowness) values than Camellia sinensis tea infusions. All infusions exhibited low turbidity levels (less than 10%). The contents of total phenols were measured as 71.8 and 74.9mg 100 ml-1 infusion in MANF and MAFF, respectively, but the MAMF tea infusion showed significantly lower total phenols (64.6mg 100ml-1 infusion). The total flavonoid contents of mulberry leaf tea infusions were lower (8.9~20.6mg 100 ml-1 infusion) than those of Camellia sinensis teas and thus had lower antioxidant capacities (DPPH: 326.8~526.9 μm trolox equivalent g-1 and FRAP: 364.6~387.6 μm trolox equivalent g-1) than Camellia sinensis teas. The amounts of γ-aminobutyric acid (GABA) and rutin were higher in fermented mulberry leaf teas; the level of GABA increased with increasing fermentation time and the content was highest in MAFF, but rutin content was highest in MAMF.

The leaves of Hippophae rhamnoides were extracted with methanol and then further fractioned with hexane, ethyl acetate, butanol and water in order to investigate biological activity. Two flavonol glycosides were isolated and identified from ethyl acetate soluble fraction, which showed the strongest antioxidant activity (RC50 = 4.33 μg/ml). Isolated two compounds have shown strong free radical scavenging activity. Especially, quercetin 3-O-glucoside (RC50 = 2.60 μg/ml) was more active than α-tocopherol (RC50 = 4.67 μg/ml). Total phenol and flavonoid contents were the highest to 4.17 and 1.14 mg/ml in the ethyl acetate soluble fraction. In addition, ethyl acetate soluble fraction was shown to the strongest α-glucosidase inhibitory activity (IC50 = 137.88 μg/ml) among the fractions. The results suggest that leaves of H. rhamnoides could be a potential source of natural antioxidant.

본 연구는 우리나라에서 자생 및 재배되고 있는 들깨와 차조기의 재배형 및 잡초형 계통들에 대하여 종자발아에 대한 변이 양상을 구명하기 위해 재배형 들깨 102계통, 잡초형 들깨 41계통 그리고 잡초형 차조기 19계통들에서 측정된 종자발아율 및 발아세는 다음과 같다. 1. 재배형 들깨는 저온처리 조건에서 1%에서 99%의 발아율을 나타내어 평균 73%±24를 나타내었으나, 비저온처리조건에서는 0%에서 98%의 발아율을 나타내어 평균 45%±26를 나타내었

1. 수수 성숙기에 간장 길이를 조사한 결과 6개체(12.2%) 가 300 cm 이상의 길이로 바이오매스가 우량한 것으로 파악되었으며, 10개체(20.4%)정도는 200~300 cm 사이의 평균 길이를 나타내었다. 2. 성숙기의 수수 줄기 직경에는 큰 차이를 보여준 개체는 없었으며, 대부분의 수수 수집종들은 2.2±0.1 cm 정도의 직경을 갖는 것으로 드러났다. 3. 본 연구에 시험한 수수 수집종들은 엽장이 60~70 cm 정도의 길이에 속하는 유전자원이 22 개체가 되는 것으로 나타났으며, 7개체는 70 cm 이상되는 것으로 나타났다. 4. 엽형은 49% 정도의 개체가 협단으로 분류되었고, 대부분의 엽맥색은 백색으로 나타났다. 5. 본 연구에 사용된 대부분의 수수의 수형은 반밀수 타원형이 많은 것으로 나타났으며, 수장은 평균적으로 20~30 cm에 달하는 것으로 나타났다.

본 연구는 수수의 성숙 종자로부터 캘러스유기 및 재분화 효율 조사하기위하여 수행되었다. 배발생 캘러스 형성을 살펴보기 위해 생장조절제 효과를 조사하였다. 수수 성숙종자로부터 캘러스 유기에 적합한 생장조절제 종류와 농도는 2 mg/L 2,4-D인 것으로 나타났다. 배발생 캘러스의 고효율 빈도는 B5 배지에 2 mg/L 2,4-D을 첨가하였을 때 가장 양호한 것으로 나타났다. 배발생 캘러스로부터 식물체 재분화는 BAP와 IBA를 넣은 MS 배지가 가장 적절한 것으로 나타났다. BAP 농도가 높을수록 신초재분화가 활발하였다. 따라서, 본 연구 결과로부터 수수 기내배양시 신초와 발근 형성에 효과적인 생장조절제 조합은 0.5 mg l-1 BAP와 0.25 mg l-1 IBA인 것으로 나타났다.

‘봄내엽1호’ 들깨 품종은 강원대학교 농장에서 2006년에서 2008년에 걸쳐 파종과 계통선발을 통해 육성하였다. 품종의 특성검정은 2006년부터 2008년까지 들깨의 적정 재배조건 아래서 3회에 걸쳐 조사하였다. ‘봄내엽1호’ 들깨 품종은 대조 품종보다 경장이 크고, 화방군수와 화방군당삭수가 많으며 잎색은 대조품종에 비해 진한 녹색을 띤다. 정유 성분 분석결과 ‘봄내엽1호’ 들깨잎의 주요성분으로는 Perilla ketone, Beta-caryophyl

In this study, we investigated antioxidant capacity and determined the phenolic and flavonoid contents using each of various solvent conditions from fermented Rhus verniciflua stem bark (F-RVS). Each extracts displayed markedly similar content of extraction yield. However, M80 extract showed a significantly higher antioxidant activity in comparison to other extract investigated. M80 exhibited strong DPPH radical scavenging activity with RC50 value of 10.5±1.4μg/ml, reducing power value 1.04 Abs (concentration of 1 mg/ml), and anti-lipid peroxidation activity value of 94.6% (concentration of 10 mg/ml). M80 extract showed the high content of total phenolic (319.7 mg GAE/ml extract) and total flavonoid (111.6 mg QE/ml extract). Phenolic and flavonoid compounds showed significant relationship in DPPH radical scavenging (R2=0.911 and 0.912, each extract) and reducing power (R2=0.903 and 0.837, each extract) from fermented R. verniciflua stem bark. However, antilipid peroxidation activity (R2=0.589 and 0.441, each extract) was not significant like DPPH radical scavenging and reducing power. Therefor the result indicated that the potential antioxidant activities and functional values were ovserved significantly at M80 solvent condition from the fermented R. verniciflua stem bark.

Anthocyanin and polyphenolic compounds present in fruits of mulberry (Morus alba L.) were determined and the influence of temperature and UV irradiation on stability of the anthocyanin-copigment complex were investigated. The copigmentation substance selected in non-anthocyanin fraction from mulberry for the study included: phenolic acid (hydroxybenzoic acid) and flavonoid (quercetin-3-O-β-D-glucopyranoside). The copigmentation effect increased with the copigment content. UV irradiation had a stronger degradation effect on the copigmentation complex than heating at 80℃. The non-anthocyanin fraction of mulberry and isolated flavonoid (quercetin-3-O-β-D-glucopyranoside) from mulberry fruit predominated over other copigment substances.