Recently, hair loss regarded as a mere means of middle-aged men due to stress and environmental pollution. The market for hair loss in Korea is about four trillion won and it is growing continuously. It is mainly made by mixing natural extracts such as medicinal plant. The purpose of this study was to investigate the effects of 70% ethanol extracts of Phellinus linteus on the growth of fibroblasts, dermal papilla cells and lipid precursors, We want to try to make a materialization. The 70% ethanol extract of P. linteus on the cell viability of Human follicle dermal papilla cell (HFDPC), adipocyte (3T3-L1), fibroblast (CCD-986sk). The result the cell viability of HFDPC was increased according to the contents of ethanol extract of P. linteus. But the 70% ethanol extract of P. linteus showed weak inhibit activity in CCD-986sk cell growth. Also, the 70%　ethanol extract of P. linteus did not inhibit the growth of 3T3-L1 cells. As a result of this study, in-vitro hair growth activity of herbal medicines for hair material development was not shown to be toxic to HFDPC and 3T3-L1 cell. As a result, the 70% ethanol extracts of P. linteus have potential to developing hair-related product. Acknowledgement : This research was supported by the Ministry of Trade, Industry & Energy(MOTIE), Korea Institute for Advancement of Technology(KIAT) through the Encouragement Program for The Industries of Economic Cooperation Region(R0005473)

The fungal mycelium-based composite is a new biomaterial to replace the existing composite material. To compete with lightweight, high-performance composites represented by fiber-reinforced plastic, various physical and chemical properties and functionality must be secured. Especially, the composite material made by using mushroom mycelium (or fruting bodies) is called mushroom plastic. Currently, Ecovative, Mycoworks, and Muskin in USA and Europe are launching new products including structural materials and leathers. Products utilizing mushroom mycelium can be launched in the market for construction materials, automobile interior materials and artificial leather substitutes. In spite of this high possibility, mass production using FMBC has not yet been reported. This presentation introduces the mycelium-based materials, a material that can replace existing plastics, inorganic building materials and animal skins in an environmentally and economically viable way, and looks at the possibility of future biomaterials by summarizing recent research contents.

The aim of this study was to analyze the effect of task-oriented progressive resistance strength training and functional electrical stimulation (FES) on the gait speed and activity of daily living (ADL) in stroke patients. Twenty one patients diagnosed with stroke were randomly divided into 3 groups: the task-oriented progressive resistance strength training group (TPRSTG, n=7), the task-oriented progressive resistance strength training with FES group (TPRSTFESG, n=7), and the control group (CG, n=7). The length of intervention for each session in all groups was 30 minutes, and was performed once a day, 5 times a week, for a total of 6 weeks. The gait speed was measured using 10m walking velocity Test (10MWT) and the ADL was measured using the functional independence measure (FIM). TPRSTFESG demonstrated the most statistically significant lapse in 10MWT (p<.05). While post-intervention analysis showed a significant difference in ADL (p<.05), further analysis between each group indicated that there were no statistically significant differences between the groups post-intervention. These findings suggest that application of both task-oriented progressive resistance strength training with FES is the most effective intervention in improving gait speed.

As a part of the effort to improve post-transfer survival rate of embryos in Korean black goats, a technique for laparoscopic uterine transfer of blastocysts was carried out. A total of 26 transferrable embryos (morula to expanded blastocysts) were transferred to 13 recipient goats via transabdominal laparoscopic method. In consequence of our hormone protocol, 65% of the recipients (13/20) were found to have synchronized estrus. After confirmation of corpus luteum in each recipient goat, a Babcock laparoscopic forceps was inserted into the lower abdominal cavity to hold a uterine horn and fasten it near the peritoneum without causing injury. Then 7.5cm long 16G IV catheter was inserted directly into the uterine lumen through the abdominal wall. After removal of the stylet of the IV catheter, the embryo transfer tube (identical in size to the stylet and loaded with blastocysts) was inserted into the uterine lumen through the catheter to unload the embryos. Of the 13 estrus synchronized recipients, 9 were transferred blastocysts and 4 were transferred molurae (2 embryos in each recipient) in uterine ipsilateral to the ovary with corpus luteum. Four of the 9 recipients which blastocysts were transferred using this method has been confirmed pregnant (44.4% pregnancy rate).

Hydrophobins are surface active proteins that are produced by filamentous fungi including mushrooms. Their ability to self-assemble into an amphipathic membrane at any hydrophilic–hydrophobic interface is most intriguing. These small secreted proteins comprise of eight conserved cysteine residues which form four disulfide bridges and an extraordinary hydrophobic patch. Hydrophobins play critical roles in fungal (and/or mushrooms) growth as structural components and in the interaction of fungi and mushrooms with the environment. The biophysical and biochemical properties of the isolated proteins are remarkable, such as strong adhesion, high surface activity and the formation of various self-assembled structures. With the increasing demands of hydrophobins from fungi and mushroom sources, production and purification in large scale is under challenge. Various applications, ranging from food industries, cosmetics, nanotechnology, biosensors and electrodes, to biomaterials and pharmaceuticals are emerging and a bright future is foreseen.

Cellulose is the most abundant organic polymer constituent of the cell wall of green plants and of various forms of algae. The complexity of lignocellulosic biomass is a major challenge in industrial research. Most mushroom species that naturally grow on soil or wood possess cellulases and the corresponding enzymatic system and, potential candidates for the direct bioconversion of softwood polysaccharides into fermentable sugars. However, there have been fewer studies on mushroom cellulases than on fungi such as Trichoderma spp., exploit the full potential of mushroom cellulases. This review will focus on the current status ofmushroom cellulase research and applications and will provide insight into promising future prospects.

Nanoemulsions containing Turmeric extract were fabricated using ultrasonication (US) system and their physicochemical properties were characterized by mean droplet size, size distribution, zeta potential, and morphology. Turmeric was firstly extracted using 50% ethanol with heating and concentrated to increase the solid content. The final curcumin content in the concentrated turmeric extract was 10.4 mg/mL. Medium chain triglyceride (MCT) oil was selected as an oil phase by the solubility test. Turmeric extract-loaded nanoemulsions (TE-NEs) were prepared with oil phase containing lecithin and water phase containing tween 80 by ultrasonication treatment. The mean droplet size of TE-NEs was significantly decreased with ultrasonication time and ranged from 66 nm to 279 nm. The optimum HLB (hydrophilic lipophilic balance) value was 10.6 which decided to obtain the smallest droplet size and the highest zeta potential of TE-NEs. TE-NEs showed good storage stability at 4°C for 30 days without any phase separation and significant change of both mean droplet size and zeta potential. Transmission electron microscope (TEM) images support that the droplet of TE-NEs was individually spherical shape and not aggregated or agglomerated until the TE concentration was less than 500% (w/w MCT oil).

‘Mantari’ is a new variety of oyster mushroom for the bottle culture. It was bred by mating with monokaryons isolated from ‘DM11732’ and ‘Chunchu-2ho’. The optimum temperature for the mycelial growth was 26~29°C on PDA medium and that for the primordia formation and the growth of fruiting body of ‘Mantari’ was 18°C and 16°C on sawdust media. It took 32 days to finish spawn running, 4 days to finish primordia formation, 3 days to finish fruitbody growth in the bottle culture. In the characteristics of fruit body, pilei were round type and dark grayish, stipe color was gray-white color and stipe shape was long and thin. The yield per bottle was 179g/900ml and was 5% higher than that of control veriety(Chunchu-2ho). As results of the physical properties of fruit body, springness, cohesive, gumminess and brittleness of stipe tissue were 96%, 76%, 160g and 15kg, respectively.

Chrysanthemums (Dendranthema grandiflorum ‘Iwanohakusen’) were grown in a greenhouse with complete nutrient solution system to investigate the effect of silicon (Si) supplement on salt induced deleterious effects in chrysanthemum plants. The experiment was conducted in plastic pots supplemented with a mixture of upland soil : leaf mold : river sand (3:3:4, v:v:v). Si and salinity were treated in combination with two levels of NaCl (0 and 100 mM) and two sources of silicon (K2SiO3, KSi and silicate fertilizer, SiF) at the same concentration (1.8 mM Si) by weekly-drenching for 12 weeks. Chrysanthemum plants supplemented with Si increased in fresh and dry matter enhancing water content and salinity tolerance. The plants grown under salt stress produced less fresh and dry matter than control plant. However, Si supplement to plants under salt stress ameliorated negative effects of salt stress. In soil, EC and NaCl increased by salt stress were mitigated by Si supplement. Salt stress significantly decreased the contents of K and P in leaf, but Si supplement under salt stress significantly recovered the decreased contents with enormous desorption of K and P in soil. Added Si significantly increased content of available SiO2 with its adsorption by salt stress in soil, which was directly related to Si accumulation in leaf. However, Si uptake by roots was suppressed by salt stress irrespective of Si supplement. Si supplement did not ameliorated the negative effects of salt stress on chlorophyll content and membrane integrity in leaf of chrysanthemum plant although significantly increased Si content in leaf, but reversed pest (Liriomyza trifolii) resistance to above-control level.

We developed the smart mosquito counter device (height 1080 mm x width 560 mm x diameter 320 mm, 220V 60Hz 30 W), which can attract the blood sucking female mosquitoes by emitting CO2 gas (300ml/min), can count the number of the captured mosquitoes by an infra-red beam area sensor, can identify the some mosquito species by analyzing mosquito image patterns using RGB (Red, Green, and Blue), and can send the captured mosquitoes’ number and pictures through the CDMA module at the real time. We operated the smart mosquito device and we could know when any mosquito peak time was in a day as well as what the mosquito species are and the captured mosquito numbers are. We found that the accuracy of the smart device was about over 95% compared the real mosquito data including numbers and images and transmitted data by CDMA. Because we can know the mosquito occurrence peak times, locations, and mosquito species by this device, we can efficiently control mosquitoes at the right time, and we can alert the public for any mosquito-born diseases based on the mosquito occurrence data. We can improve the quality of mosquito vector control with the functions of this device and we will develop the smart device to identify more mosquito species.

Four pluriparous Korean black goat does were superovulated with FSH and mated with fertile bucks. Anesthetized animals were placed in lateral recumbency, then size 8 Foley catheter was inserted into the uterus through the cervix under the vaginal speculum and the balloon was inflated to fix the catheter in the uterine body. The opposite end of the catheter was connected to a 3-way and a flushing medium was infused into the uterus. Modified Dubecco’s PBS with 1% FBS was used as the flushing medium. Four goats were allocated in two groups depending on the type of medium infusion into uterus. Injection group; the flushing medium was injected into uterus and the infused medium was collected by to-and-fro method using a syringe. Gravity-flow group; the flushing medium was allowed to enter the uterus by gravity flow by lifting the medium bottle and drained out of the uterus into a collecting tube. All four goats had catheter inserted through the cervix and uteri flushed successfully. The volume (recovery rate) of recovered medium varied considerably from 87 ml/200 ml (43.5%) to 148 ml/160 ml (92.5%). Nine embryos/ova in total were recovered from Gravity-flow group goats. Although the embryo recovery rate was low, the possibility of a transcervical embryo recovery in Korean black goat had been proven in this preliminary experiment.

This study examined a timed cloze test for evaluating English proficiency in second language (L2) experimental research. Forty-five Korean college students were randomly assigned to either a timed or untimed condition. In the timed condition, the participants read the sentences of the text one phrase at a time, using the self-paced, cumulative, moving-window reading paradigm, and their reading time (RT) and accuracy were measured. In an untimed condition, the participants carried out a typical pencil-and-paper cloze test. Linear mixed-effect models were used to analyze the data. Although the accuracy data did not indicate any significant results, the RT data showed that the participants responded faster when they selected accurate answers and they answered function words rather than content words; also, as the participants’ TOEIC scores increased, a marginally significant RT difference was observed. A significant correlation was also found between the cloze test and high TOEIC scores in the timed condition. The results showed that the timed cloze test used in the study can provide useful data for L2 experimental research in measuring L2 proficiency.