Two-spotted spider mite, Tetranychus urticae (Koch) is one of the serious pests in economically important crops such as strawberry and cucumber and so on. Acaricides have been used as the main control agents. This study was conducted to test the synergistic effects of the Beauveria bassiana GHA, which has been registered for whitefly and thrips, and five pesticides (abamectin, acrinathrin, bifenthrin imidacloprid, dinotefuran, and indoxacarb, which are commonly used pesticides in strawberry in Korea), on the two-spotted spider mite T. urticae. Five tested pesticides did not inhibit spore germination and mycelial growth of B. bassiana. Pesticides were applied to potted strawberry plants at four different treatments (recommended concentration, 1/5 recommended concentration, 1/5 recommended concentration + GHA(108 conidia/ml), and only GHA(108 conidia/ml). Mortality in larvae of two-spotted spider mite was 12% in GHA 5 day after treatment. Mortality in abamectin treated T. urticae was 98.5% and 100% at 1/5 recommended concentration and recommended concentration, respectively, 3 days after treatment. Acrinathrin, indoxacarbe, dinotefuran and difenthrin imidacloprid caused 60%, 14%, 16% and 91% mortality at recommended concentration 5 day after treatment. The tested five pesticides and B. bassiana GHA had no synergistic effect.

Pine wood nematode (PWN), Bursaphelenchus xylophilus is associated with the pine wilt disease and transmitted by pine sawyer, Monochamus alternatus. Because pine sawyer has one-year life cycle, one natural infection of PWN is occurred a year. Therefore, artificial propagation method of PWN is needed to improve experiment associated with PWN. In this study, effect of diameter, paraffin sealing of twig and dosage on pine wood nematode reproduction in Japanese black pine, Pinus thunbergii. PWN reproduction was compared in twigs of P. thunbergii and P. densiflora. Numbers of reproduced PWN were higher with decreasing diameter of twig. Distance (5 and 10 cm) from inoculation site of PWN did not influence reproduction of PWN. Reproduced numbers of PWN were higher in the paraffin-sealing twig than non-sealing twig. Dosage of PWN influenced reproduction of PWN. Reproduction rate was the highest at the rate of 10 IJs (13.7 and 61.1-fold increasing in P. densiflora and P. thunbergii, respectively 30 days later) whereas lowest at the rate of 1000 Ijs (1.1 and 0.7-fold increasing in P. densiflora and P. thunbergii, respectively 30 days later). Numbers of reproduced PWN were more in P. thunbergii than P. densiflora.

As an attempt to develop a value-added food product, purple sweet potato powder was added in a model system of steamed bread as a healthy food ingredient and physicochemical properties such as moisture content, specific volume, spread ratio, color, texture as well as consumer preferences on the attributes such as uniformity, color, flavor, elasticity, chewiness, taste, and overall preference were evaluated. Moisture content ranged from 44.16 to 44.55% (wet basis) and appeared independent on the level of purple sweet potato (PSP) powder incorporation. As a result of the addition of PSP powder, the specific volume of steamed bread decreased from 3.22 to 2.55 mL/g, and value of 4.5% sample was significantly lower than other samples (p<0.05). On the other hand, spread ratio ranged from 2.01 to 2.53, and appeared to decrease as the PSP powder concentration increased (p<0.05), indicating a significant improvement. Lightness (L*) decreased significantly as the PSP powder content increased (p<0.05) for both dough and skin of the steamed bread. In addition, an increasing trend in redness (a*-value) and a decreasing trend in yellowness (b*-value) were noticed. Firmness increased significantly with the addition of PSP powder regardless of concentration (p <0.05); however, firmness was not significantly different among samples containing 1.5-4.5% PSP powder (p>0.05). Consumer acceptance test indicated that incorporation of 3% PSP powder in the formulation of steamed breads would be recommended.

This study aims to reveal how EA affects BAX and NF-kB involved in cell deaths from global ischemia, and to do this, observes the changes of BAX and NF-kB caused by EA application after transient global ischemia. The experimental method is to give rise to global ischemia and apply EA to 27 SD rats with the particulars of being six-week-old, male, around-300 gram-weighing, and adapted to laboratory environment for more than a week, and divide them into three groups, that is, GV20 EA group(n=9), L14 EA group(n=9), no-treatment GI group(n=9), and then observe their changes of BAX and NF-kB at the time lapse of 6 hours, 9 hours and 12 hours after ischemia, using western blotting. The numerical decrease of BAX expression at the time lapse of 9 hours after EA application, though not statistically significant, was observed in GV20 EA group and L14 EA group, and the NF-kB expression appeared statistically significant decrease in GV20 EA group and L14 EA group, but the expression was higher in the group with EA application. Therefore, EA application at the early phase of global ischemia is considered to affect BAX and NF-kB and play a positive role in decreasing apoptosis and cell deaths by inflammation.

The majority of strokes are caused by ischemia and result in brain tissue damage, leading to problems of the central nervous system including hemiparesis, dysfunction of language and consciousness, and dysfunction of perception. The purpose of this study was to investigate the effects of Poly(ADP-ribose) polymerase(PARP) on necrosis in neuronal cells that have undergone needle electrode electrical stimulation(NEES) prior to induction of ischemia. Ischemia was induced in male SD rats(body weight 300g) by occlusion of the common carotid artery for 5 min, after which the blood was reperfused. After induction of brain ischemia, NEES was applied to Zusanli(ST 36), at 12, 24 and 48 hours. Protein expression was investigated using immuno-reactive cells, which react to PARP antibodies in cerebral nerve cells, and Western blotting. The results were as follows: In the cerebral cortex, the number of PARP reactive cells after 24 hours significantly decreased(p<.05) in the NEES group compared to the GI group. PARP expression after 24 hours significantly decreased(p<.05) in the NEES group compared to the GI group. As a result, NEES showed the greatest effect on necrosis- related PARP immuno-reactive cells 24 hours after ischemia, indicating necrosis inhibition, blocking of neural cell death, and protection of neural cells. Based on the results of this study, NEES can be an effective method of treating dysfunction and improving function of neuronal cells in brain damage caused by ischemia.

본 연구에서는 azoxymethane (AOM)과 dextran sodium sulfate (DSS)로 유도된 대장 발암과정에 대한 셀레늄의 방어 효과를 조사하였다. 셀레늄 결핍(0.02 ppm Se), 정상(0.1 ppm Se), 과다(0.5 ppm Se)사료를 12주간 식이로 급여하여 혈액검사와 대장암 발생의 초기단계인 aberrant crypt foci (ACF)수를 측정했으며, 암 발생율을 조사하였다. ICP-AES 를 사용하여 간의 셀레늄 농도를 측정하였으며, 또한 셀레늄포함 항산화효소인 glutathione peroxidase (GPx) 활성을 알아보았다. 또한 TUNEL assay와 PCNA, β-catenin에 대한 면역조직 염색을 수행하였다. ACF 수 및 종양 발생률에 있어서, 셀레늄과다사료를 급여한 군이 정상셀레늄사료를 급여한 군보다 낮았으며, 셀레늄결핍사료를 급여한 군은 오히려 ACF 수 및 종양 발생률이 높았다. GPx 활성은 셀레늄의 섭취가 과다한 군에서 높게 나타났으며, 이 때, TUNEL 에서 apoptotic positive cell이 증가하는 것을 확인했다. 또 한 셀레늄의 섭취가 과다한 군에서 PCNA와 β-catenin의 발현이 감소됨을 볼 수 있었다. 본 마우스 모델실험에서 셀레늄은 여러 기전에 의해 대장암 발생을 억제할 수 있을 것으로 사료된다.

Wrinkles are an outward sign of cutaneous aging appearing preferentially on ultraviolet B (UVB)-exposed areas. The anti-wrinkle effects of herbal extracts were investigated in an animal model. Female albino hairless mice (HR/ICR) were randomly allocated to the control group (non-irradiated vehicle), positive control group (UVB irradiated-vehicle), and two herbal extract mixture groups (HE-1 and HE-2). HE-1 included Glycyrrhizae radix, Rhei Rhizoma, Cornus officinalis, and Sesami semeni, and HE-2 included Swertia pseudo-chinensis, Sophora flavescens, Scutellaria baicalensis, and Salvia miltiorrhiza. The herbal extract mixtures were pre-treated dorsally with 0.2 ml per individual five times per week for four weeks prior to the start of UVB irradiation. At the fifth week, the animals were exposed to UVB irradiation for a subsequent eight weeks, three times per week. The intensity of irradiation showed a gradual increase, from 30 mJ/cm 2 to 240 mJ/cm2 (1 MED: 60 mJ/cm2 ). Dorsal skin samples were stained with H&E in order to examine the epidermal thickness. In addition, Masson-Trichrome staining was performed for determination of the amount of collagen fiber. Treatments with HE-1&2 resulted in an increase in the amount of collagen fiber, a better appearance, and fewer wrinkles, compared with the positive control. As determined by hydroxyproline assay, treatments with HE-1&2 led to a significant increase in the amount of collagen, compared with the positive control group (p<0.05). Chronic UVB irradiation to skin of hairless mice resulted in an increase in expression of matrix metalloproteinase-1 (MMP-1), however, treatments with HE-1&2 tended to decrease the expression of MMP-1. These results indicate that the herbal extracts used in this study have a preventive effect on UVB-induced wrinkle formation in a hairless mouse model, due in part to inhibition of MMP-1 expression and increment of collagen amount.

Since physical therapy was first introduced in Korea, it has been 50 years past and Korean physical therapy has made rapid progress. However, history or educational system of Korean physical therapy is still not known worldwide. Now, for Korean physical therapy to go beyond Asia and leap toward the world, endless studies, efforts and publicity are required. Korean physical therapy first began by missionaries dispatched from other countries like America and Canada with Korean War, which occurred in 1950. After the War, Korean Physical Therapy is devel˗ oped very fast. Korean Physical Therapy Association was founded in October 1, 1965 and many physical therapist were discharged. Korea became a full member of World Confederation of Physical Therapy(WCPT) in 1974, and held the 2nd Asia Pacific Confederation of Physical Therapy assembly in 1984 and WCPT assembly in 2005. Today, in 2010, licensed physical therapists are about 35,000 and there are physical therapy departments in 74 universities, and 17 uni˗ versities have master's or doctor's degree courses. And there are many academic journals(more than 10) related to physical therapy that are published. Many Korean physical therapists are working at various countries like America or Australia, and are acknowledged with excellent treatment technology and academic studies. This thesis aims to shed new light to the history and educational system reorganization of Korean phys˗ ical therapy and introduce it to the world, and establish the historical foundation to develop Korean physical therapy into the international level.

The purpose of this study was to investigate perceived health status, activities of daily living and depression of the elderly in nursing facil˗ ities and to identify correlations among them. The collected data is to improve healthy life for the aged people in communities. This study was performed by using of questionnaire which was consisted of perceived health status, activity of daily living(ADL) and depression. The survey was conducted by 180 aged people at nursing homes. The results of perceived health status show that 64.9% of elderly feel very bad or bad, 61.6% of elderly have a degree of independent level of activity of daily living(ADL) and 48.6% of elderly have a degree of depression. There were statically revealed meaningful correlation between ability of activity of daily living(ADL) and perceived health status, ability of activity of daily living(ADL) and depression. This study about connection among perceived health status, activity of daily living(ADL) and depression is necessary for number of the affil˗ iation function of elderly at nursing homes and development of inter˗ vention programs concerned about depression are necessary.

The purpose of this study is to investigate the effects of group exer˗ cise program on depression in the elders with dementia. Subjects of this research were selected from the patients of Hyoja hospital(Yong˗ in, Korea). They were elders with dementia having minor to moderate degrees of cognitive function. Twenty-four subjects were randomly assigned into two groups, each with 12 people; exercise group and the control group. The control group only performed general physio˗ therapy, while the exercise group performed exercise program and general physiotherapy for 8 weeks. Depression of the exercise group and the control group were measured at baseline before the study, at 4 weeks, and at 8 weeks after the study. Depression was measured by Geriatric Depression Scale Short Form Korea Version(GDSSF-K). Firstly, when comparing the levels of depression within the same group, depression was significantly lower in the exercise group, after the group exercise program. However, depression of the control group did not show significant changes before or after the study. Secondly, when comparing the levels of depression of the control group and the exercise group, differences between the levels meas˗ ured before the study and 8 weeks after the study, was a statistically significant decrease of depression. Thirdly, effects of group exercise program according to the applied period were analyzed showing 8 weeks of group exercise to be more beneficial than 4 weeks. People who performed continuous group exercise program showed decrease in depression compared to the absence of group exercise program. This can infer beneficial effects of group exercise program. Group exercise program had desirable influence on decreasing the level of depression.

In organic agriculture, various cover-crops have been used to control weeds. In this study, we have investigated the effect of Vicia tetrasperma (L.) Schred. (Eolchigiwandu) which is native to on major insect pests of pepper. Redpepper seedlings at 8 leaves stage were transplanted in 20th May 2009 into experimental field located in the farm of the department of agricultural biology, Suwon, Korea. A cover-crop cultivation plot was compared with a control plot mulched by black plastic-film. Density of aphids and damaged fruits by oriental tobacco budworm, Helicoverpa assulta, were surveyed ten times from 21 days after transplanting (DAT) to 82 DAT. Fifty and forty pepper plants were sampled to count the density of aphids and damaged fruits by oriental tobacco budworm per a plant, respectively. In current study, three aphid species namely, cotton aphid, Aphis gossypii, green peach aphid, Myzus persicae, potato aphid, Macrosiphum euphorbiae were collected. Overally, the density of aphids on pepper in the control plot was higher than the cover-crop plot. Especially, aphid density increased up to 67.44±26.8 in early stage of control plot, whereas aphid was not found in cover-crop plot. The rate of fruit damage by oriental tobacco budworm was significantly higher in the cover-crop plot than the control plot in early stage of pepper, however damaged fruit rate of pepper in the control plot was significantly higher since the middle of July.

The devastating insect pests of the tomato (Lycopersicon esculentum) were surveyed in environmentally friendly (no-pesticide and organic) greenhouses located in the provinces of Gyeonggi, Gyeongsang, Gangwon, Chungcheong and Jeju. Whitefly, leaf miner, aphid and moth were recorded as the major pests in these surveys. Among them, whitefly was the most serious pest of greenhouse cultivated tomato and major species was Trialeurodes vaporariorum. The population densities of T. vaporariorum was 0~36 nymph per leaf. Population of moths Spodoptera litura were the highest in summer season. Population of the whitefly was considered to be influenced by the previous cultural methods. Farmers in organic cultivation, used insect natural enemy, commercial EFAM (Environment Friendly Agricultural Material), yellow sticky trap or home made pesticides. Commercial microbial pesticides were applied to evaluate their insecticidal activities against whitefly. The results revealed that microbial pesticides exhibited 65.3 to 100% whitefly nymph mortality. Further, pesticidal activities of Cooking Oil and Yolk (COY), plant extracts and neem oil were evaluated against other tomato pests as well.

Although hair disorders are not life threatening, a lot of people who suffer hair loss and/or hair thinning is increasing in accordance with changes in lifestyle and nutritional balance. The aim of this study was to examine the effects of herbal extracts on hair regrowth in C3H/HeJ mice. There were 6 experimental groups including distilled water (D.W.), 50% ethanol (a vehicle control), 3% minoxidil (a positive control), and 3 kinds of herbal extracts mixtures (C, D & E). The test compounds included followings; C : Glycyrrhizae radix, Rhei Rhizoma, Cornus officinalis and Sesami semeni, D : Viticus fructus, Pulsatilla chinensis, Gardenia fructus and Artemisiae argyi herb, E : Swertia pseudo-chinensis, Sophora flavescens Scutellaria baicalensis and Salvia miltiorrhiza. The animals were shaved with an electric clipper. The test compounds were daily treated to dorsal skin with 0.2 ml per mouse for 3 weeks. The topical application of the E test solution accelerated hair regrowth after 10 days faster than that of the positive and vehicle controls. The activities of alkaline phosphatase (ALP) and γ-glutamyl transpeptidase significantly were increased in all the treatment groups after 3 weeks, compared with D.W. group. Especially, the E test solution notably increased ALP activity compared with positive or vehicle control group. Epidermal growth factor (EGF) and vascular endothelial growth factor (VEGF) were increased in all the treatment groups after 3 weeks compared with D.W. group. These results suggest that the herbal extracts have hair regrowth effect by increasing enzyme activities and growth factors and it can be useful for treatment for alopecia in humans.

This experiment was conducted to compare forage production and grass intakes of warm season grasses for grazing horses in Jeju. The experimental field located at 450m altitude(Jeju Horse Training Center of Korea Racing Association) was divided into 3different treatment plots(3replications) without a fence between treatments, and sown by three treatments(bermudagrass, bahiagrass, bermudagrass+bahiagrass mixture). Three castrated adult horse a were kept on pasture from the 1 of October to the end of October without supplementary concentrates. 'Bahiagrass' cultivar showed the highest dry matter (DM) yield with 8,887㎏/㏊ than those in other treatments (bermudagrass 8,699, bermudagrass+bahiagrass mixture 8,385㎏/㏊). The percentage of grass intakes was higher in the treatment of bahiagrass(92%) than those in other treatments(bermudagrass+bahiagrass mixture 89, bermudagrass78%). The results indicate that the warm season grasses of bermudagrass, bahiagrass, bermudagrass+bahiagrass mixture can be used for grazing pasture of horses in the Jeju.

In the present study, we have used an annealing-control-primer (ACP)-based differentially display RT-PCR method to identify salt-stress-induced differentially expressed genes (DEGs) in barley leaves. Using 120 ACPs, a total of 11 up-regulated genes were identified and sequenced. Temporal expression patterns of some up-regulated DEGs in response to salt stress were further analyzed by Northern blot analysis. The possible roles of these identified genes are discussed within the context of their putative role in response to salt stress. Thus, the identification of some novel genes-such as SnRK1-type protein kinase; 17 kDa, class I, small heat shock protein; and RNase S-like protein precursor genes-may offer a new avenue for better understanding the salt stress response in plants, knowledge which might be helpful for developing future strategies.

Managing the fatty acid composition of grazing ruminant diets could lead to meat and milk products t㏊t have higher conjugated linoleic acid (CLA) concentrations, but forage fatty acid dynamics must be more fully understood for a range of forages before grazing systems can be specified. The fatty acid profiles of 23 different forages, including grasses, legumes and wild forage plants were determined. Linolenic(C18:3, 41.1~58.6% of fatty acid), linoleic(C18:2, 10.1~27.4% of fatty acid), and palmitic(C16:0, 9.8~18.0% of fatty acid) acids were the most abundant fatty acids in all species, together representing ~96% of the fatty acids present. Concentrations of fatty acids declined as plants developed, but the fraction contribution of each fatty acid to total fatty acids remained relatively stable over time.