In this study, we investigated the microbial community of oyster mushrooms at different growth stages at the species level. Gram-positive bacteria were predominant in the presterilized medium. On the other hand, Gram-negative bacteria were predominant in the culture-completed medium, post-harvest medium, and fruiting bodies. In addition, Pseudomonas tolaasii, which is known to cause disease in mushrooms, was confirmed in the cultured medium, post-harvest medium, and fruiting bodies, and it was determined that the mycelium culture stage was contaminated, and the reason why no disease occurred was Sphingobacterium psychroaquaticum. It was confirmed that this was because the growth of Pseudomonas tolaasii was suppressed by producing a component called tolacin. As a result of confirming the diversity of microorganisms, it was confirmed that the presterilization medium contains a variety of microorganisms compared to other growth stages, and the diversity decreases in the order of culture completion medium, fruiting body, and post-harvest medium. showed a trend. As a result of microbial similarity analysis, it was confirmed that the cultured medium and the post-harvest medium showed similar microbial communities, and in the case of fruiting bodies, there were some similarities but overall differences.
This study analyzed the effects of different nitrogen sources in substrate composition on the growth of Pleurotus ostreatus, as well as the subsequent changes in flavor and antioxidant activity. The T2, composed of poplar sawdust, beet pulp, cotton seed dregs, and cotton seed coat in a ratio of 40:20:20:20, exhibited the highest yield at 156.6 g. The total polyphenol content and ABTS and DPPH radical scavenging activities were 8.25 mg GAE/g, 70%, and 49%, respectively, showing higher radical scavenging activity compared to the Control and T1. Additionally, varying nitrogen content resulted in distinct aroma patterns and is presumed to influence taste profiles such as sourness, umami, and saltiness.
Since ships are exposed to noise and vibration for a long time in seas isolated from land, the stress of workers is high, requiring studies on the improvement of the problem. In addition, the health of crew members on fishing vessels, unlike commercial ships, carrying out fishing activities is closely related to fishing performance. However, considering that most of IMO's guidelines apply to merchant ships, provisions should be made for vibration in accommodation areas of fishing vessels. Accordingly, this study, applying the revised ISO 20283-5:2016 standard and through experiment, investigated the vibration characteristics of the accommodation area for each operating conditions of a 1000-ton trawler fishing vessel. Within the scope of the study and the full-ahead mode, the vibration was highest in the engine control room (4.17mm/s) while the acceptable standard was satisfied sufficiently in all conditions. Application of the merchant vessel vibration standard to fishing vessel is expected to improve the working environment of fishing vessel crew members.
In this study, we examined the effects of gamma irradiation dosage on the mycelial growth of Auricularia auriculajudae and performed analyses of fruiting body yield, growth characteristics, taste, fragrance, and mineral composition. Assessments of mycelial growth in response to gamma irradiation at different intensities revealed an enhancement in the growth of fungi exposed to irradiation at 200 Gy. Fruiting body yield was also highest at 200 Gy, followed by 800 Gy and the control group. On the basis of these observations, we subsequently applied gamma ray doses of 200 and 800 Gy to examine the effects of irradiation on fungal quality characteristics. In terms of the taste of fruiting bodies, we detected no significant differences among the control, 200 Gy, and 800 Gy groups. Contrastingly, with respect to fragrance, we found that fungi treated with 200 Gy were characterized by a pattern that differed from those of the control and other treatment groups. Furthermore, whereas we detected no significant difference among treatments with respect total dietary fiber content, calcium content was found to be higher in the treatment groups compared with the control group, with the highest content being measured in fungi exposed to 800 Gy irradiation. Copper content was confirmed to be higher in the control group, whereas there were no significant differences between the fungi irradiated with 200 and 800 Gy. Contrastingly, the highest levels of zinc were detected in response to 200 Gy irradiation, followed by 800 Gy. Collectively, our findings thus indicate that gamma irradiation can contribute to promoting increases in the fruiting body yield and mineral contents of mushrooms.
Recently, the International Maritime Organization is strengthening regulations for ships operating in polar regions. Hence, insulated multi-core tubes as components for vessels operating in extreme cold need to be investigated in various aspects. Furthermore, the demand for research on electric propulsion vessels is also increasingly growing. Thus, to utilize a 4-core insulated multi-core tube with glass wool as insulation, which was previously developed for ships operating in polar regions, as a water-cooled electrical cable, this study conducted an experiment on the temperature change when water at normal temperature 25℃ was supplied as a coolant under the overcurrent varied from 10A to 25A. As a result, the temperature increase of the core in 10A condition was 3.3℃, but it increased to 13.05℃ in the 25A condition. This showed that a temperature difference of approximately 9.75℃ occurred according to the overcurrent load. However, the coolant inlet and outlet temperatures were relatively uniform around 1℃ in all conditions. This suggests that increasing the residence time by proper control of the coolant flow in the future could achieve a higher cooling effect.
Recently, the government has provided support such as entering new markets, expanding sales channels, and supporting manpower, not just in the form of funding, to efficiently and effectively support limited national resources to improve corporate performance. In this study, we tried to find out the effect of government support for companies that have benefited from the Excellent Technology Research Center Project (ATC Project) and the World Class 300 project using propensity score matching. As a result of the analysis, the effect of government support for the ATC project became visible after the appointment period, while the effect of the World Class 300 project was insignificant. This means that when the size of the company is small, the effect of government support is more pronounced. This suggests that in order to maximize the effectiveness of government support, appropriate national policy interventions such as government innovation funding are needed when the size of the company is small. In this study, differences in the timing, performance indicators, and company size of policy support effects were found in the growth stage of a company from a mid- to long-term time series perspective, suggesting that support policies based on this need to be adjusted and redesigned.
A new brown variety of Flammulina velutipes, referred to as ‘Asakgold,’ was bred by crossing two monokaryons isolated from the “Geumhyang2ho” and “Garlmoe” varieties. The pileus color of the new variety is light brown and its shape is hemispherical. During bottle cultivation, the period necessary for mycelial growth was 40 days, 9 days for primordia formation and 15 days for fruiting body growth. The total cultivation period was 64 days, which was similar to that of the control variety “Yeoreumhyang1ho.” The pileus of the new variety was similar and the stipe was thinner and longer than that of the control. The number of valid stipes per bottle was 495, which was 37 % higher than that of the control (362), and the yield was 214 g, 16 % higher than that of the control (185 g). The -glucan content was 28.69 %, which was observed to be 1.6 times more than that of the control.
In this study, the growth characteristics of Lentinula edodes were confirmed by bean sprout waste(BW) as an alternative raw material for rice bran. The mycelium growth of Sanjo701, a major cultivation variety of L. edodes, was compared between a medium mixed with 8:2(v/v) of oak sawdust and a medium mixed with BW 50% and BW 100%. The mycelium growth in BW 50% was 13.5 cm. Compared to the control, BW 50% increased the diameter of the pileus by 1.6 cm. Additionally, the length of the pileus decreased by 0.4 cm when comparing the growth of the fruit body. In contrast, at BW 50%, the diameter of the pileus decreased by 9.6 cm and the length of the stipe decreased by 1.4 cm. According to analysis of the constituent amino acids, BW 50% showed a lower overall nutritional content than the control, whereas BW 100% had a lower amino acid content than the control. However, glutamic acid and aspartic acid, which are flavor-enhancing ingredients, were observed at levels of 3.954 mg/g and 1.436 mg/g, respectively, in BW 100%. Therefore, if bean sprout by-products are efficiently processed and utilized, it is believed that they will be beneficial to farmers as a substitute for rice bran and reduce the cost of manufacturing substrate
A new dark brown variety of Flammulina velutipes, referred to as ‘Asakchoco’, was bred by crossing two monokaryons isolated from the ‘Yeoreumhyang2ho’ and ‘Guemhyang2ho’ varieties. The pileus color of the new variety is dark brown and the stipe color is ivory, with uniformity from the upper section to the base. In addition, the primordia formation is uniform compared to that of the control, and the advantage of this is that the pileus does not become deformed after the primordia formation. During bottle cultivation, the period necessary for mycelial growth was 40 d, for primordia formation was 8 d, and for fruiting body growth was 16 d. The total cultivation period was 64 d, which was 2 d shorter than that of the control. The pileus was smaller and thicker and the stipe diameter was the same, but the stipe length was longer than that of the control. The number of valid stipes per bottle was 441, 24% higher than that of the control (357), and the yield was 197 g, 19% higher than that of the control (166 g).
In this study, we aimed to compare the mycelial growth of Pleurotus ostreatus after medium supplementation with various amino acids at different concentrations to select the optimal medium nutrient composition for mycelial growth. The mycelial growth of P. ostreatus was investigated after adding four amino acids (tryptophan, threonine, methionine, and lysine) at 0.5% or 1% to the medium.The rate of P. ostreatus mycelial growthwas faster in the potato dextrose agar (PDA) medium supplemented with threonine at 0.5% or 1% than that of the control, whereas it was inhibited by tryptophan treatment. Supplementation of sawdust mediumwith all amino acids, except tryptophan, at 0.5% did not alter the mycelial growth, compared to the controls. However, addition of any amino acid to sawdust medium at a higher concentration (1%) inhibited the mycelial growth. The laccase acitivity of P. ostreatus mycelium cultured in PDA medium was the highest when threonine was added, and the lowest when tryptophane was added, consistent with the results of the mycelial growth. Therefore, the addition of threonine, methionine, or lysine to PDA medium at a concentration of 0.5-1%was effective for increasing the mycelial growth of P. ostreatus; however, it inhibited mycelial growth insawdust medium, suggesting that the effects of amino acids dependedon the medium nutrient composition.
Thermoelectric materials and devices are energy-harvesting devices that can effectively recycle waste heat into electricity. Thermoelectric power generation is widely used in factories, engines, and even in human bodies as they continuously generate heat. However, thermoelectric elements exhibit poor performance and low energy efficiency; research is being conducted to find new materials or improve the thermoelectric performance of existing materials, that is, by ensuring a high figure-of-merit (zT) value. For increasing zT, higher σ (electrical conductivity) and S (Seebeck coefficient) and a lower к (thermal conductivity) are required. Here, interface engineering by atomic layer deposition (ALD) is used to increase zT of n-type BiTeSe (BTS) thermoelectric powders. ALD of the BTS powders is performed in a rotary-type ALD reactor, and 40 to 100 ALD cycles of ZnO thin films are conducted at 100oC. The physical and chemical properties and thermoelectric performance of the ALD-coated BTS powders and pellets are characterized. It is revealed that electrical conductivity and thermal conductivity are decoupled, and thus, zT of ALD-coated BTS pellets is increased by more than 60% compared to that of the uncoated BTS pellets. This result can be utilized in a novel method for improving the thermoelectric efficiency in materials processing.
This study investigated the culture characteristics of Cryptoporus volvatus, whichis grow naturally in Korea, to determine the suitable environmental conditions for its cultivation. The physiological characteristics of the mycelia were assessed according to the cultivation conditions, to determine the optimal conditions for artificial cultivation. The visual characteristics of the hyphae of Cryptoporus volvatus KACC52303 included an irregular and uneven surface and a fuzzy or cotton-like texture. Under the microscope, its microstructure showed pre-chlamydospore formation, but no clamps were seen. The appropriate culture temperature was found to be a medium/high temperature of approximately 25–30oC, and the optimal pH was found to have a wide range from weakly acidic (pH 4) to neutral (pH 7). In the optimal nutrient source experiment, hyphal growth was shown to be fair in a mixed medium with 2.5% dextrin as the carbon source and 0.1% yeast extract as the organic nitrogen source. Among the various amino acids, organic acids, and inorganic salts tested, the fastest hyphal growth was observed in the presence of leucine, acetic acid or gluconic acid, and KCl or KH2PO4, respectively. The column test showed that the best mycelial growth occurred in a mixed medium of 80% pine sawdust, 10% rice bran, and 10% corncob sawdust.
This study was conducted to investigate the growth and antioxidant activity of Pleurotus ostreatus mycelia grown in medium supplemented with Aronia. The diameter and dry weight of the mycelia were increased in the medium supplemented with Aronia compared with the untreated medium. The total polyphenol content of mycelia grown in medium supplemented with Aronia and untreated medium was 6.54 mg GAE/g and 5.77 mg GAE/g, respectively. The 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity of mycelia grown in medium supplemented with Aronia and untreated medium was 61.81% and 49.65%, respectively. Moreover, the 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity of mycelia grown in medium supplemented with Aronia and untreated medium was 59.83% and 52.66%, respectively. These results confirmed that P. ostreatus mycelial growth and antioxidant activity were increased when Aronia was added to the culture medium.
UN 출범 이후 80년이 되어가는 2022년의 시점에도 추축국으로 표상 되는 독일 및 일본과 관련된 우리의 역사적, 정치적 인식에는 극명한 차 이 및 대비가 존재한다. 즉, 독일의 경우 전후처리 문제에 있어 일본과 대조되는 진정한 반성 및 후속 대처의 모범적 사례로서 우리 사회에 긍정 적으로 다가옴은 주지의 사실이다. 이는 일단, 조선 말기 이래 유무형의 막대한 피해를 일제로부터 경험하였음에 기인한다. 그리고 해방 및 대한 민국 정부 수립 이후 지금까지 국제법상 식민지 청산 문제가 계속되는 현 실 속에서 인권 담론과 유리된 일본의 자세가 대한민국 각계의 분노를 촉 발하였으며, 그와 대비되는 실행을 모색하도록 촉발한 측면이 존재한다. 하지만 거듭 소환되는 독일의 ‘사례는 침략 및 과거사에 대한 전향적 반성 및 대처의 모범이 될 만한지, 과연 보편적이고 명백한 실행인지 점 검할 필요가 있다. 체코 및 근년의 나미비아에 대한 실행을 보면 독일은 역사적 과오나 국제법상 국가책임 자체는 인정하되, 구체적 배상문제와 관련하여서는 피해자 측에 대한 양비론적 비판이나 국내외법상 근거를 엄 격히 요구하는 경향이 있으며, 관습 국제법이나 지엽적 절차법의 문제를 들어 제소를 방해하거나 배상(reparation)의 형식에 반대하기도 한다. 다 만, 나미비아 피해자 유족들은 최근 독일에 대한 미국내 주권면제 관련 소송에서 패소하였으나 이 패소는 헛된 것이 아니며, 오히려 구독일 제국 의 나미비아 헤레로족 및 나마족 학살 사건의 청산 문제 해결을 촉발하기 도 하였다. 또, 독일의 승소는 역설적으로 역사적 가해국의 배상문제에 관한 소극성, 양면성을 보여주는 계기로 작용한다는 점에서 중-동부 유럽 에서의 독일의 수정주의적 시각과도 접점이 존재한다. 우리로서는 향후 잠재적인 사안에 있어서 일본에 대항하는 주권면제 부 인론 등의 논리를 어떻게 확보할 것인가? 설사, 패소한 사안에 대해서도 전투에서 지고 전쟁에서 이기는 효과로 전환 시킬 여지는 없는가? 이런 부분에 대한 고민이 필요함을 언급하면서, 이번 연구에서의 문제 제기에 갈음하고자 한다.