본 시험은 포도부산물의 첨가가 젖소의 산유량, 유성분 및 혈액 성상에 미치는 영향을 조사하기 위하여 실시하였다. 비유중기 젖소 8두(평균 월령: 89.8, 평균 유기: 164.4, 평균 산차: 2.8산)를 공시하였으며, 포도부산물을 첨가하지 않은 대조구와 1일 1 kg을 첨가한 시험구로 두 처리구를 설정하여 3주간 급여하였다. 유지방, 유단백, 유당 및 총 고형물 함량은 처리간에 차이가 없었다. 그러나 산유량은 포도부산물 을 첨가한 처리구가 23.13 kg/d으로 대조구의 21.16 kg/d에 비해 높은 반면(p<0.05), 체세포 수는 대조구가 포도부산물 처리구에 비해 높았다(p<0.05). 혈중 glucose, alanine aminotransferase, total protein 및 blood urea nitrogen 함량은 처리구간 차이가 없었으나, AST와 총콜레스테롤 함량은 대조구 (123.75 IU/L, 200.82 mg/dl)가 포도부산물 처리구(95.34 IU/L, 180.63 mg/dl)에 비해 높았다(p<0.05). 이상의 결과에서, 다양한 생리활성을 가진 포도부산물은 젖소를 위한 기능성 사료로 이용 가능성이 높을 것으로 사료된다.

This study was conducted to investigate the effects on in situ ruminal degradation of feed protein sources (soybean meal, P-SBM; dried distillers grain with solubles, P- DDGS; wheat bran, P-WB) treated with protease as compared with conventional feed protein sources (soybean meal, SBM; dried distillers grain with solubles, DDGS; wheat bran, WB). There was no significant change in the chemical composition and amino acid profiles of enzyme treatment protein sources compared with the non-treated groups (p<0.05). But for treatment groups, the solid content and total amino acids were reduced by increasing the moisture content due to proteolytic conditions. On the entire incubation time in situ ruminal degradation rate of dry matter appeared higher in treatment groups compared to control groups (p<0.05), and that of the treatment groups suspended during 48 hours were in the order of P-SBM (97.70%), P-WB (74.26%) and P- DDGS (72.39%). In particular, DM degradation rate of enzyme treated DDGS significantly increased to 43.62%, 45.99%, 55.97%, 69.87% and 72.39%, respectively, incubated during 3, 6, 12, 24 and 48 hours in rumen (p<0.05). Also protein degradation rate of P-WB and P-SBM significantly decreased compared to their respective non-treated sources; however, by contrast, in DDGS it was increased. For P-SBM, protein degradation rate significantly decreased at 6 and 12 hours, and for P-DDGS it was increased at 3 and 6 hours of suspension times in rumen (p<0.05). In particular, protein degradation rate of enzyme treated group suspended for 48 hours were in the order of P-SBM (91.81%), P-WB (86.36%) and P-DDGS (58.87%). This result suggests that protease treatment of feed protein sources might be utilized to increase the bypass ratio into post-rumen for wheat bran, soybean meal, and to improve the utilization of feed protein.

A study was conducted to investigate the efficiency of recipient for Embryo Transfer (ET) in Holstein Heifers. A total of 193 heifers (Age 14-20 months and body weight, 250-400 Kg) was assigned in 2 groups (natural ovulation cycle group and Hormone-induced ovulation synchronization group) based on the development of corpus luteum (CL) and uterus where 28.49% (55/193) heifers were in normal estrous. The ET technique was applied in both of the groups to transfer the embryo in the recipients and evaluated their efficiency. In vivo frozen embryos were used for ET at the blastocyst stage. Results showed that according to recipient preparation method the conception rates were 22.72% (10/44) and 40.26% (60/149) in hormone-induced ovulation synchronization group and natural ovulation cycle group, respectively. The pregnancy rate of heifers was significantly higher (p<0.05) during the first time ET compared to repeat ET; however, recipient showed no significant difference in CL development in both side. The conception rate were 31.03% (9/29), 37.75% (57/151) and 15.38% (2/13) at day 6, 7 and 8, respectively after the CL development of the heifers. The conception rate was significantly higher (p<0.05) in the right side compared to left side of the CL development. In addition, during ET anesthesia group and non anesthesia group conception rate of the recipient were 27.63% (21/76) and 41.88% (49/117), respectively.

This study was carried out to elucidate changes in milk production, milk components and milk product properties as influenced by organic and conventional farming system. Milk production in transient organic feeding was lower by 7.6% than that in conventional feeding and also milk fat, milk protein content, solids not fat and somatic cell count were lower than conventional farming system. But, milk lactose content in transient organic feeding was higher than that in conventional feeding. Ca and Fe content in milk in organic feeding tended to be higher compared to conventional feeding. Linoleic acid and unsaturated fatty acids in milk in organic feeding was higher than those in conventional feeding and properties of milk products were improved in organic milk compared to conventional milk.

본 시험은 섬유질배합사료(TMR)의 에너지 및 단백질 수준이 다를 경우, 비유초기 유산양의 사료섭취량 및 유생산에 미치는 영향을 조사하기 위하여 수행하였다. 시험 동물은 자넨종 생후 16개월령 전후의 초산 착유양 24두를 시험구별 6마리씩 공시하였으며, TMR의 에너지와 단백질 수준은 대조구(Control)가 TDN 67.0%, CP 13.9% (NRC, 2007), 에너지 증량구(T1)는 TDN 73.7%, CP 13.9%, 단백질 증량구(T2)는 T

The objective of this study was to determine physiological and mechanical factors effecting on the pregnancy rates following artificial insemination in dairy cattle. Estrus for artificial insemination of cows was checked whether the outflow of mucus from the elements was out flow or not. There were no significant difference of gestation rates with mucus release (31.76%) and without mucus release (24.03%). The pregnancy rates were 39.02% in 10~20mm of follicle size group and 27.08% (p<0.05) in 20~30mm diameter of the follicles. There were not different pregnancy rates between twice inseminated dairy cattle and more than 3 times inseminated cattle. The pregnancy rate was 28.57% under automatic milking system. In contrast, under artificial milking system pregnancy rate was 56.85%. Two systems significantly (p<0.05) were different. These results suggest that pregnancy rates were not effected by physiological system, but mechanical condition.

Substantial efforts have been made to manipulate ruminal environment in a hope to enhance ruminal fermentation efficiency for better ruminant productivity. Some of examples are methane inhibitors, antibiotics, microbial enzymes, fatty acids and/or lipid feeding, buffering agents, ionophores and probiotics. Of these efforts, the non-ionic surfactant (NIS) has been known for its stimulation to release enzymes from a range of anaerobic microbes. A series of studies were conducted 1) to evaluate NIS diluted with water and ethanol on in vitro ruminal fermentation and 2) to determine the influence of diluted NIS on digestibility of feedstuffs. In first experiment (Exp. 1), NIS was diluted with water or ethanol to measure its effects on in vitro microbial growth, ruminal enzyme activities and gas production by mixed ruminal microbial culture. The NIS was diluted with water or ethanol separately in a 1:5 ratio (w/v). Water and ethanol-diluted NIS with wheat flour were added with rice straw-based mixed ruminal microbial cultures at the rate of 2 ㎎ NIS/16 ㎖ McDougall buffer plus 4 ml ruminal fluid solution. The mixed ruminal microbial culture was run without any NIS addition as control. Addition of NIS either diluted with water or ethanol has significantly reduced the gas production in mixed ruminal microbial culture at 12 and 24 h of incubation. At 48 h post incubation, gas production was the highest with the addition of ethanol diluted NIS followed by water-diluted NIS and control. Carboxy methyl cellulase activity in rice straw-based mixed ruminal bacterial culture was significantly higher with the addition of ethanol-diluted NIS compared with the addition of water-diluted NIS and control at 24 and 72 h post incubation. In second in vitro experiment (Exp. 2), effects of addition of ethanol diluted NIS on dry matter (DM) digestibility of alfalfa hay, gas production, pH and cellular growth in mixed ruminal microbial culture were examined. Alfalfa hay based mixed ruminal microbial culture without any NIS addition was run as a control. The pH of mixed ruminal microbial culture was significantly lower than control at all post incubation sampling hours. In vitro DM digestibility of alfalfa hay was significantly higher with the addition of NIS compared with control. Gas production was significantly less with NIS addition compared with control at all post incubation sampling hours. Microbial growth in mixed ruminal microbial culture was significantly increased with the addition of NIS compared to control.

유산양의 에너지 및 단백질 이용성을 조사하기 위하여 대조구(A), TDN 증량구(B), 조단백질 증량구(C) 그리고 TDN 및 조단백질 증량구(D)로 구분하여 섭취량, 소화율과 질소 및 에너지 이용성을 시험하였다. 공시축은 유산양(Saanen, 평균체중 17.5kg, female) 12두를 공시하였고, 2008년 10월 20일부터 11월 9일까지 충남대학교 부설동물사육장에서 수행하였다. 건물 섭취량은 D구에서 가장 높았으며 B구가 C구보다 유의적으로 높

본 연구의 목적은 축우용 TMR 사용이 점차 증가하고 있으나 TMR의 영양성분을 측정하고자 할 경우 실험실 분석에 의존하므로 많은 비용과 시간이 소요되어 농가 현장에서 활용하기가 어려움에 따라 NIRS(Near InfraRed Spectroscopy, 근적외선분광분석기)을 이용하여 TMR에 대한 신속하고 간편하게 사료가치를 평가하기 위한 검량선을 작성하기 위하여 실시하였다. 근적외선 분광분석법은 친환경 분석으로서 시약의 사용이 전혀 없고, 폐수 및 유해

본 연구는 홀스타인, 한국재래산양 및 한우유로부터 glycomacropeptide(GMP)를 분리하였으며, 각 GMP의 trypsin 가수분해물의 혈소판응집 억제 효과를 in vitro 상에서 알아보았다. 홀스타인, 한국재래산양 및 한우의 GMP는 분자량이 모두 약 20 KDa이었으며, sialic acid 함량은 각각 36.86±2.36, 37.98±1.27 및 31.19±1.87㎍/mg이었다. 또한 모든 개체의 GMP에서 tyrosine이 검출되었다. 홀스타인, 한국재래산양 및 한우 GMP의 trypsin 가수분해물에 의한 혈소판 응집 억제율은 반응 30초에 4.02, 5.51 및 12.77%로 각각 나타나 시간이 경과할수록 감소하는 경향을 보였다. 혈소판의 현미경 관찰 결과 가수분해물 첨가 후 혈소판 수가 증가하였으나, 첨가 후 30초가 경과한 시점부터 혈소판 수가 감소하기 시작하여 120초 후에는 관찰 할 수 없었다. 본 실험 결과 bovine 및 caprine GMP의 trypsin 가수분해물에서 혈소판 응집을 억제할 수 있는 small peptide가 있는 것으로 생각되며, 향후 이러한 연구는 심근경색증 및 뇌혈전증을 예방할 수 있는 생리활성 물질로 이용될 수 있을 것이라 생각된다.

본 시험은 국내 TMR 배합소에 대한 실태조사를 통하여 문제점을 파악하고 개선방향을 제시하기 위하여 수행하였다. TMR 배합소 20개소 조사결과 조사 배합소의 65%인 13개소가 사료배합비 작성을 외부에 의뢰하고 있는 것으로 조사되었으며, 배합비 작성 등 컨설턴트 비용으로 월 평균 만원 정도를 지출하고 있어, 컨설팅 비용에 대한 부담으로 경영비를 상승시키는 요인이 되는 것으로 나타났다. 국내 유통 TMR에는 에너지가인 TDN가를 표기하고 있지 않아 농가

본 연구의 목적은 TMR 제조 시 물을 첨가하여 수분 함량을 높여줄 경우 수분 함량(15, 35, 50%)과 발효제(Lactobacillus plantarum) 첨가유무에 따라 저장방법(자연노출과 밀봉보관) 경과시간별(자연노출 : 0, 6, 12, 24, 48, 72시간, 밀봉보관 : 0, 2, 6, 10, 14, 18일) 사료가치 변화를 조사할 목적으로 실시하였으며 그 결과는 다음과 같다. 자연노출 상태로 저장한 경우, 수분 함량이 적을수록 외관상태가