This study was conducted to evaluate the quality variation of Lithospermum radix on the pigment contents and antioxidant activities according to different growth stages and areas of cultivation. Acetylshikonin contents showed the tendency to decrease gradually from the middle of July (0.28%) to the end of August (0.05%) and then the content was increased again to the end of October (0.25%). Shikonin content was detected as small amount of about 0.009% during the period. The weight of plants was increased from the end of September to the end of October and showed the highest value as 19.8 g on October 25. ROS scavenging activity was the highest in the early of October as IC50 value of 0.11 μg/mL. Lithospermum radix of September showed lower ROS scavenging activities than those of other growth stages as IC50 value of 1.02 and 0.49 μg/mL on September 9 and September 27, respectively. Among 17 areas cultivated Lithospermum radix, 10 areas (59%) showed 0.05-0.10% of acetylshikonin contents and 3 areas (18%) were measured to contain 0.16-0.26% of acetylshikonin.

The study was done to investigate the effects of the extracts from the different parts of Lythrum salicaria (LS) on liver protective activities in chronically alcohol-treated rats. SD male rats except normal animals were administrated with alcohol (30ml of 30%~40% ethanol/kg/day) and the extracts (300 mg/kg/day) for 10 weeks. Chronic alcohol administration decreased body weight, high density lipoprotein (HDL)-cholesterol and the reduced form-glutathione (GSH), whereas increased the ethanol content, glutamic-oxaloacetic transaminase (GOT), total cholesterol, low density lipoprotein (LDL)- cholesterol, triglyceride in blood/serum and the ratio of the oxidized form of glutathione (GSSG) and total GSH (GSSG/total GSH) in liver tissue. Groups treated with the extracts of leaf, root and stem, showed decrease in GOT, total cholesterol and GSSG/total GSH and increase in hepatic aldehyde dehydrogenase (ALDH), total GSH and serum albumin. Administration with the root extract of LS decreased blood ethanol content compared with the other part extracts. But, serum triglyceride values in rats treated with root and stem extract were higher than that of the negative control animals. Flower extract-fed group showed decrease in body weight and serum triglyceride, but increase in the ratio of GOT and glutamic-pyruvic transaminase (GPT), and GSSG/total GSH. From the results, we conclude that the extracts of root and leaf among the plant parts of LS might be useful for the amelioration of the chronic alcohol-induced liver demage of rat.

The study was conducted to investigate candidate materials as anti-inflammation agent from plant resources. Activities of 33 plant parts extracts with the final concentration of 5μg/ml were evaluated on the several inflammation-related markers such as the release of proinflammatoty cytokine [tumor necrosis factor-alpha (TNF-α) & interleukin-6 (IL-6)], nitric oxide (NO), the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and inhibitor of nuclear factor kappa-B alpha (Ik-Bα) in lipopolysaccharide (LPS)-treated RAW 264.7 cells. The extracts in the final concentration of 10 μg/ml were also screened on peroxynitrite (ONOO-) scavenging activity. Eleven extracts selected from the screening assay were verified on the inhibition activity on peroxynitrite and total reactive species oxygen (ROS) in the several concentrations. As results, Alpinia officinarum Hance (rhizome), Inula britannica var. chinensis Regel (flower), Ulmus arvifolia Jacq (trunk peel) and Aster scaber Thunb. (aerial part) showed comparatively potent anti-inflammatory activities in vitro cells or chemical level systems, and then these four plant parts should be studied on the antiinflammatory mechanism by further studies.

This paper aims to clarify the structure of Korean Afterthought Constructions and the identity of the gap in the construction. The main points can be summarized as follows. First, the syntactic head Ω hosts afterthought element. Second, Ω can occur in CP cycle and some expressions having independent function such as presentation constructions. Third, the gap of the construction is copy, which means that the afterthought construction is a kind of movement phenomena. Finally, the structure in which afterthought elements adjoin to Ω head is immune from the right roof condition which regulates a rightward movement.

This study was conducted to investigate the flora of Mt. Jang located at Haeundae-Gu in Busan. The survey for the flora was carried out from Oct. 2008 to Sep. 2009. The results were obtained as follows. The flora consists of 5 forms, 22 varieties, 238 species, 198 genera, 91 families, 47 orders, 7 classes, and 3 divisions as total 265 taxa. The typical communities were Osmunda japonica com., Stephanandra incisa com., Gleichenia japonica com., Eurya japonica-Pinus thunbergii com., Styrax japonica-Pinus thunbergii com., and Smilax china com. which were distributed along Forest Bathing Area and Yangun Falls, Miscanthus sinensis for. purpurascens com., Carpinus turczaibovii var. coreana com and Pseudosasa japonica were distributed around the top of mountain. According to the ecological characteristic species, the evergreen plants were observed 15 taxa 14 genera 13 families, the naturalized plants 18 taxa 17 genera 10 families, the specific plants categorized by Ministry of Environment 20 taxa 18 genera 18 families, the endangered plant 1 specie Utricularia yakusimensis, and the cultivated plants were observed 21 taxa 18 genera 14 families.

본 연구는 자연하천에서 보 설치에 따른 배수위 계산을 실시하여 여러 가지 수리특성인자들의 결과와 비교해 보았다. 그 결과, 자연수위에 따른 통수단면적보다 수위증가로 인한 통수단면적의 영향이 가장 큰 것으로 분석되었다. 그러나 유속을 포함한 다른 수리특성인자의 영향은 다소 적은 것으로 분석되었다. 따라서 보(weir)를 설치할 경우 다른 수리특성인자보다 수위상승에 따른 통수단면의 통수능력이 먼저 검토되어야 할 것이다. 또한 자연하도에 비해 수중보 설치에 의한 상류의 수위상승 종단거리가 예상치보다 매우 크게 나타나고 있었기 때문에 보 설치에 따른 종단거리의 영향을 파악하여 제방설계를 실시해야 할 것으로 판단된다. 보의 설치는 수위증가로 인한 용수확보, 수변공간 확보 등 많은 이점이 있으나, 홍수시의 하도통수능력 고려, 오염물질의 퇴적으로 인한 수질오염 방지 그리고 갈수시에 하천의 유지용수 확보 등을 고려한 신중한 분석·검토를 해야 할 것으로 사료된다.

본 논문에서는 안동댐 조정지에서 낙동강 하구 사이의 낙동강 구간에 대하여 4대강 사업에 의한 하도 준설과 보(수문) 건설이 낙동강 본류의 지표면에 대한 빗방울과 유수의침식 운반 퇴적작용에 의해 상류에서 이송된 유사가 쌓인 충적토를 하천이 흐름의 특성에 따라 기하학적형태가 스스로 변화하고 자유수면과 하상 및 하안이 모두 가변적인 경계로 작용되었다. 만곡부에는 사주가 감소하였고 지류합류부에서는 하도폐섹 교량과 보에서는 국부세굴과 퇴적과 침식이 반복적으로 일어났다 이에 대한 결론으로 하도관리방안은 만곡부에서는 하도선형조성,저수로확장 사주에서는 저수로 식생역 제거 농경지철거 선택적인 하상골재채취를 해야한다

This text was analyzed and investigated the vegetation and floristic composition by cluster analysis and classification of phytosociological method, to evaluate the species composition, habitat environment and community structure of Liriope platyphylla and Liriope spicata group in Korea. The southeast slope gradient of the habitat of L. platyphylla and L. spicata was 6.7 to 8.4%, and the habitat altitude of L. platyphylla (41.0 m), L. spicata (114.9 m) was different. Habitat distribution of L. spicata was broader than L. platyphylla. Appearing plants of L. platyphylla and L. spicata group was 58 taxa, 99 taxa, respectively, and Coverage of tree layer was 87.5%, 92.5% respectively. In genus Liriope group, the highest appearing frequency of plant grow in the moist valley as Quercus serrata. Thus, plants of genus Liriope growth was better in moist shade. The vegetation of L. platyphylla group was classified into Quercus serrata community, Castanopsis sieboldii community, Pinus densiflora community and Pinus thunbergii community, and the Liriope spicata group was classified into Quercus serrata community, Quercus alien community, Quercus acutissima community, Prunus verecunda community, Robinia pseudoacacia community, Pinus densiflora community and Pinus thunbergii community. In genus Liriope group, Quercus serrata and Pinus densiflora communities was the closest the similarities.

Root extract of Lythrum salicaria reported a hepato-protective effect on CCl4-induced liver toxicity of rat was prepared into fractions such as n-hexane up layer (HA), n-hexane down layer (HB), diethyl ether (E), ethylacetate (EA), n-butanol (B) and water (W). Fractions prepared were tested their activities in vitro and in vivo condition. All of the fractions showed effective antioxidant asctivities on DPPH radical and CuSO4-induced oxidation of human low density lipoprotein and E fraction showed the highest inhibitory effect (98.1% at 50 μg/ml) on linoleic acid autoxidation at 40℃, which was more effective than α-tocopherol (82.4%). Five fractions (H = HA plus HB, E, EA, B, and W, 150 mg/kg/day) were fed into Sprague Dawley, male rats for 4 days, which were intoxicated with intra-peritoneal injection of carbon tetrachloride (1 ml/kg in corn oil) at the 4th day and were sacrificed in 24 hrs. Serum tumor necrosis factor-alpha (TNF-α), a proinflammatory cytokine, elevated with CCl4-intoxication in negative control group (83 pg/ml) was significantly decreased in E fraction-supplemented group (18 pg/ml). Cu, Zn-superoxide dismutase (SOD) activity increased in negative control group (0.12 U/mg protein) was decreased in E fraction (0.07 U/mg protein). From the results, it is suggested that ether fraction from root extract of L. salicaria would be a potent antioxidant candidate for ameliorating liver injury induced by chemical intoxicant.

For the investigation of possibility as a useful functional material, different parts of Lythrum salicaria L. harvested at four growth stages were studied in the aspect of bleeding characteristics, chemical composition and in vitro activity. Weights (g/plant) of L. salicaria plant parts were high in order to stem 〉 leaf 〉 flower 〉 root at the best growth time. Crude lipids (3.59~4.30%) and crude proteins (14.7~23.5%) of L. salicaria leaves were the highest among the other plant parts showed from 0.08~3.54%, and 4.0~21.9%, respectively. Free sugars (2.9~4.2%) and crude ash (11.9~14.8) of leaves also showed the highest value. Free radical scavenging activities of L. salicaria root on 2,2-diphenyl-1-picrylhydrazyl showed from 43.5 μg/ml to 47.6 μg/ml as IC50 which were followed by those of flower, leaf, and stem. Root of L. salicaria tested at 100 μg/ml also showed the most efficient inhibitory effect on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in murine macrophage RAW264.7 cells. Cell viability of the plant parts tested by 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyl tetrazoliumbromide (MTT) assay was high in order to flower, leaf, root, and stem. Total phenol content measured as tannic acid equivalent showed the highest value in flower. In conclusion, among the plant parts, especially leaf of L. salicaria, was rich in the chemical components, and showed efficient antioxidant/inhibitory activity on free radical and NO production, and was expected to be a functional material candidate.

한국산 쑥속 분류군의 계통분류학적 연구를 위해 Nuclear ribosome DNA의 ITS 염기서열 분석을 실시하였다. 정렬된 염기의 총 길이는 635~643 bp이며, ITS1과 ITS2 부위의 길이는 각각 251~255 bp와 217~222 bp로 나타났다. 염기서열 변이를 보이는 site는 95개로 확인되었다. 그 중 ITS1이 35개, ITS2가 26개로 총 72개의 site가 계통학적으로 유효한 것으로 나타남으로써 ITS1이 ITS2보다 종 분화의 변이가 다양하게 발생하는 것으로 확인되었다. ITS 염기서열을 기초한 계통학적 분석은 쑥속 내에 5개의 Clade를 형성하였다. 그 결과 자방이 퇴화된 분류군들(사철쑥, 제비쑥, 섬쑥, 갯제비쑥)이 하나의 분계조(Clade 1)를 형성함으로써 아속 수준(Subgen. Dracunculus)으로 취급되는 결과를 뒷받침 하였다. 애기비쑥과 큰비쑥은 거의 동일한 유전적 정보를 보였으며(Boostrap 99%), 한국산 참쑥의 학명은 재고 되어야할 것으로 사료된다. 또한, 강화약쑥(A. sp.)은 황해쑥과 매우 가까운 상동성을 보였다(Boostrap 89%). 따라서, 형태적 형질의 변이가 다소 연속적인 쑥속은 DNA 염기서열에 기초한 분자계통학적 연구가 유용한 방법으로 판단되며, 본 ITS 연구결과는 한국산 쑥속의 계통분류를 이해하는데 유용한 형질로 기여할 것으로 기대된다.

This study describes an efficient approach to the development of DNA markers for use in distinguishing the Scrophularia species that have been used as useful medicinal crops. In order to distinguish Scrophularia species, DNA sequences of rpl-5 region in mitochondrial DNA of Scrophularia species were analysed for detecting sequence variations, and the PCR-RFLP method was applied for developing practicable DNA marker patterns. Several DNA variations were detected by the sequence comparison of rpl-5 region among Scrophularia species. Genetic relationship analysis of Scrophularia species was carried out based on these DNA variations. DNA variations of rpl-5 region were revealed that it was significantly efficient in genetic relationship analysis of Scrophularia species. In addition, Scrophularia species tested in this study were completely discriminated by four polymorphic genotypes by PCR-RFLP combined with Tsp509 I (^AATT) restriction enzyme. Our results suggested that DNA sequence variations of rpl-5 region were sufficiently useful for genetic relationship analysis of Scrophularia species. Polymorphic genotypes by PCR-RFLP using the Tsp509 I enzyme will be useful for discrimination of Scrophularia species as a practicable DNA markers.

In order to development medicinal Artemisia herbs of high quality, Korean A. annua L. were investigated with its ecological and morphological characteristics, and basic statistical data of agronomic characteristics. This species, which is annual herb, is mainly distributed to marginal land, riverside, roadside, grassland. Ecological niche is low species in competition of the others. Its pollination is basically anemogamous, but is frequency pollinated by insects. This species is characterized as tap root, 1~3 pinnate compound leaf of ovate or narrowly ovate, stem is green, erect and solitary. Inflorescence is paniculate, receptacle is not hair. Capitulum, consist of ray floret and disk floret, is subglobose shape. Additionally, this species could be easily discriminated from related Artemisia herbs by the capitulum size. Ray floret is female, disk floret is bisexual. Flowering season is from August to October. Seed is achene unattached hair. Stem length ranged from 179 cm to 225 cm, and stem diameter and number of branch were 17.14 ± 1.68 mm, 2.43 ± 0.51 mm, respectively. Length and width of leaf were 14.5 ± 0.5 cm, 15.0 ± 1.0 cm, and leaf number of main stem were 48.06 ± 10.57 cm, respectively. Fresh weight of aerial parts and root were 364.7 ± 14.1 g, 32.6 ± 5.1 g, and its dry weight were 136.6 ± 10.0 g, 14.9 ± 2.34 g, respectively.

레이더 빔 차폐는 송신된 레이더 빔이 지형 및 차폐물로 인해 부분 혹은 완전히 차단되는 현상으로 강수량 추정시 과소추정의 주된 원인이 된다. 본 연구에서는 레이더 누적반사도지도를 이용하여 지형에 의한 부분차폐영역의 반사도를 보정하였다. 누적반사도지도는 레이더 반사도를 누적하고, 공간적으로 균일한 레이더 반사도 장을 가정하여 작성하며, 빔 차폐를 분석하는데 유용한 자료이다. 차폐분석을 통해 차폐보정지도를 작성하고, 태풍과 장마 사례에 적용하여 차폐가 발생

본 연구는 흰넓적다리 붉은쥐(Apodemus speciosus peninsulae)의 세정관 상피주기와 정자세포의 발달 단계를 광학 현미경을 사용하여 조사하였다. 세정관 상피주기는 정모세포의 분화단계와 정자세포의 발달단계를 기초로 하여 9 stages로 나누었다. Ad형 정원세포의 경우 모든 단계에서 관찰되었다. Ap형의 정원세포는 I 단계, In형의 정원세포는 II와 III 단계, B형의 정원세포는 IV 단계에서 관찰되었다. 제 1 감수분열의 전기에

For providing basic data on estuary conservation and pattern of vegetation succession of sand bar, the flora of delta was investigated from March 2004 to August 2006 in the Nakdong River estuary of Korea. The flora in the eight surveyed areas was composed of total 159 taxa that belonged to 46 families, 123 genera, 133 species, 1 subspecies, 20 varieties, and 5 forms. Among them, naturalized plants and evergreen broad trees were 31 taxa and 5 taxa, respectively. The communities of main species at delta of estuary were composed of 16 species such as Rosa rugusa community, Scirpus triqueter community, Phragmites communis community, Carex kobomugi community, Digitaria ciliaris community. Especially, Rosa rugusa community which are important to establish the southern limit line of distribution as the northern factor was distributed through Jinwoodo. The succession of main vegetation community in the new delta (Doyodeung) was in order of Carex scabrifolia, Scirpus triqueter, Phragmites communis, Imperata cylindrica var. koenigii and Miscanthus sinensis var. purpurascens. Therefore, the succession order of the surveyed area seems to predict the pattern of the vegetation succession of another deltas in Nakdong River estuary.