The association between the COMT rs4680 (G>A, Val-158-Met) polymorphism and the risk of fibromyalgia has been investigated in previous studies, but the results are controversial. Therefore, a meta-analysis has been performed to confirm the association between COMT rs4680 (G>A, Val-158-Met) polymorphism and the risk of fibromyalgia in this study. Our study includes eleven case-control studies with 2,909 individuals comprised of 1,365 fibromyalgia patients and 1,544 control subjects. The regression analysis was performed using the random effects model or the fixed effects model and OR with the corresponding 95 % CI was calculated for the allele, recessive, dominant, over-dominant, co-dominant 1, and co-dominant 2 model. No statistical significant associations were observed between COMT rs4680 (G>A, Val-158-Met) polymorphism and risk of fibromyalgia in allele model (P-value = 1.00), recessive model (P-value = 1.00), dominant model (P-value = 0.54), co-dominant 1 model (P-value = 1.00) and co-dominant 2 model (P-value = 1.00). In conclusion, our meta-analysis showed that the COMT rs4680(G>A, Val-158-Met) polymorphism might not be genetic risk factor for the fibromyalgia.

Salivary gland adenocarcinoma(AdCa NOS) is one of the major causes of mortality among malignant salivary gland tumors. New therapeutic measure are needed to improve the outcome for patients with AdCa NOS because current therapy does not significantly improve survival rates. Transglutaminase 2(TGase 2) was implicated in forming cross-linked protein polymer, apoptosis and matrix interaction. And also TGase 2 expression is up-regulated in proliferation, migration, invasion, and metastasis of cancer cells. shRNA which has emerged as an effective method to target specific genes for silencing has provided new opportunities for cancer therapy. But there has been rarely reported using shRNA-TGase 2 transfection in AdCa NOS. The purpose of this study were to examine the specific inhibition of TGase 2 mRNA and protein expression by siRNA transfection of TGase 2 through RT-PCR and immunoslot blotting, and to study proliferation, migration and invasion assay of SGT cell line from AdCa NOS. Cell cycle analysis showed that the downregulation of shRNA-TGase 2 caused the accumulation of cells in the sub-G0/G1 phase. In migration assay, suppressing shRNA-TGase 2 inhibited the capacity of the cells to migrate compared to parental cells. In invasion assay, cells transfected with shRNA-TGase 2 decreased in invasion when compared to SGT and vector transfected cells. shRNA-TGase 2 expressing plasmids efficiently downregulated TGase 2 mRNA and TGase 2 protein expression. It suggested that the shRNA-TGase 2 targeting system against TGase 2 could have a therapeutic potentiality for malignant salivary gland tumors, especially in inhibiting and/or preventing cancer cell proliferation, migration and invasion.

The need for skin health care is newly recognized in accordance with the importance and needs of skin care due to harmonious interpersonal relation. In this study, collected 202 questionnaire sheets in Seoul were analyzed by SPSS 18.0 program through data coding and data cleaning, and frequency analysis for general characteristics was done. Heredity, environmental factors and management condition of acne, and the differences according to general characteristics were analyzed by Crosstabs. Mainly, women used skin care shop and their age groups were varied from twenties to fifties. Recognition of skin trouble were compound(42.1%), and significant statistical difference(p<.05), while oily or compound to man and compound or acne skin to woman were observed. First occurrence of acne was generally middle school(33.7%). Facial acne progress areas was cheek(36.1%), forehead(30.7%), chin(28.2%) and neck(5.0%) in descending order, and present state of acne was in progress(60.9%). Etiologic factors were stress(39.1%), pre and post-menstruation(22.8%), environment(20.8%), heredity(17.3%). There was significant statistical difference between gender(p<.01). Treatment places were at dermatologist(45.0%) and special skin care shop(27.2%). The most reliable place for treatment was special skin care shop(44.6%) and dermatologist(42.6%). It suggested that in acne of skin trouble, age groups of customers could be varied among male and female and in order to meet such requirements of customers, high quality service ought to be provided so that acne care could satisfy customers with the differentiated and careful service.

Despite existing chemotherapy and surgical resection strategies, salivary gland adenocarcinoma(AdCa NOS) is one of the major causes of mortality among malignant salivary gland tumors. New therapeutic measure are needed to improve the outcome for patients with AdCa. Overexpression of urokinase-type plasminogen activator receptor/urokinase-type plasminogen activator(uPAR-uPA) has been implicated in progression and metastasis of oral cancer. RNA interference(RNAi) which has emerged as an effective method to target specific genes for silencing has provided new opportunities for cancer therapy. But there has been rarely reported using RNAi-uPAR/uPA transfection in salivary gland AdCa. The purpose of this study were to examine the specific inhibition of uPAR/uPA mRNA and protein expression by RNAi transfection of uPAR/uPA through RT-PCR and Immunoslot blot, and to study tumor cell proliferation activity, adhesion, invasion and migration of SGT cell line in vitro compared to the controls. In adhesion assay, cells transfected with RNAi-uPAR/uPA inhibited markedly adhesion to vitronectin compared to parental cells. Angiogenic assays revealed a significant decrease in the angiogenic potential of SGT cells downregulated by both uPAR and uPA. In migration assay, suppressing uPAR and uPA inhibited the capacity of the cells to migrate compared to parental cells. In invasion assay, cells transfected with RNAi-uPAR/uPA showed the maximum decrease in invasion when compared to all other treatment conditions. RNAi expressing plasmids efficiently downregulated mRNA and protein expression of uPAR and uPA. Cell cycle analysis showed that the simultaneous downregulation of uPAR and uPA caused the accumulation of cells in the sub-G0/G1 phase in SGT cells. Immunoslot blot analysis revealed that downregulation of uPAR and uPA caused the prominent activation of caspase 8. It suggested that the RNAi targeting of the uPAR/uPA system could have a therapeutic potentiality for malignant salivary gland tumors.

Nicotine of tobacco component has a controversial impact in the clinical outcome of dental implants. Although numerous nicotine effects on bone healing around implants have been presented, it is rarely reported in vitro study about normal human osteoblast(NHost) from oral and maxillofacial area at the surface of implants. The purpose of the present study was to evaluate the effect of nicotine on the proliferation and differentiation response of NHost to plasmatic and salivary levels of nicotine reported in smokers at the surface of screw-type plasma-sprayed titanium implants. NHosts were seeded on the surface of titanium implants and cultured for 21 days in α-MEM supplemented with 10% FBS, 50mg/ml ascorbic acid, 5mM β-glycerophosphate and 100nM dexamethasone. Seeded implants were exposed to various nicotine concentration(0.05-0.5mg/ml) from 1 to 21 days, and characterized for cell morphology, proliferation, differentiation, alkaline phosphatase(ALP) activity and ionized calcium concentration(Cai) of medium. Continuous exposure to higher nicotine concentration(above 0.3mg/ml) induced a dose- and time-dependent vacuolation of the cytoplasm, and a tendency to detach from the implant surface. 0.05mg/ml(lower nicotine concentration) did not cause significant effects in the cell proliferation and ALP activity. 0.1-0.2mg/ml caused evident dose-dependent effects in increased cell proliferation, ALP activity and earlier onset of matrix mineralization at levels up to 0.2mg/ml, while a dose-dependent inhibitory effect at 0.3-0.5mg/ml. Cai concentration of control group was decreased at 14 days. Increased Cai concentration at 0.1-0.2mg/ml, decreased Cai concentration at 0.3mg/ml and no change at 0.5mg/ml during the culture period were seen. It suggested that nicotine concentration could paly an role in modulating NHost activity as a contributing factor associated with proliferation and differentiation of NHost at the surface of implants.

Hyaluronic acid is natural polysaccharide existing around skin or cartilage, and is known as a moisturizing factor. It is expected that hyaluronic acid cosmetics market will continue to grow in the future, as its use and demand increases, like medical supplies and health food besides cosmetics containing hyaluronic acid. However, There are few present studies on content in cosmetics containing hyaluronic acid. The purpose of this study was to evaluate satisfaction and examine what effect was produced on sebum and moisture in skin after applying to cosmetics the hyaluronic acid helpful in maintaining moisture in skin at 5%, 20% and 50% concentration. For 71 days, a clinical trial in a total 39 persons living in the national capital region was carried out. And sebum and moisture was measured through skin diagnosis system A-ONE TAB before trial and each day when clinical trial was conducted for hyaluronic acid content group. Besides, this study was to examine subjects' general characteristics, eating habits, and living habits, and satisfaction after the clinical trial. This study analyzed a difference according to the time to measure the skin condition among hyaluronic acid content groups by utilizing statistical package program SPSS v. 21.0 through data coding and data cleaning process. There was no statistically significant difference in hyaluronic acid content according to any of demographic characteristics, eating habits and living habits among groups (p>.05). With regard to hyaluronic acid content, there was no statistically significant difference in moisture and sebum prior to clinical trial in T zone and U zone among groups (p>.05). A change of moisture in T zone among groups according to hyaluronic acid content showed statistically significant difference in the 2nd week (F =3.636, p<.05). And the highest level was shown in 50%(44.15±2.672), being followed by 20%(43.15±2.340), and 5%(41.62±2.219). Accordingly, it was shown that, as hyaluronic acid content relatively increased, the moisture in T zone increased. In the results of analyzing the change of moisture in U zone, the change of sebum in T zone and U zone by group according to hyaluronic acid content, no statistically significant difference was shown in sebum level of T zone by group(p>.05). For analyzing the subjective satisfaction after clinical trial among groups according to hyaluronic acid content, satisfaction score was the highest in 'skin moistness' (4.38±0.650) for 5% group, 'skin moistness' (4.69±0.480) for 20% group and 'skin texture smoothness' (4.54±0.519) for 50% group. And no statistically significant difference was shown in each subjective satisfaction by group according to hyaluronic acid content(p>.05). It was thought that the results of skin condition and subjective satisfaction survey according to hyaluronic acid content would be baseline data for developing cosmetics containing the hyaluronic acid and marketing a skin moisturizer.

As human's average lifespan is gradually increased, the interest in the quality of life and health is also increasing. More and more customers like to use the facial manual technique as a way to live a healthy life, instead of using it as measures to simply improve skin troubles or to have beautiful skin. The facial manual technique performed in the skin care stage is closely related to the decrease of stress. In order to verify the effects of facial manual technique on the decrease of stress, an experiment was conducted in the same method by dividing 16 women residing in Seoul into four groups, from September 1st to September 30th 2016. To understand their general characteristics, perceptual stress, and satisfaction with facial manual technique through questionnaire, the frequency analysis and descriptive statistics were performed by using SPSS 22.0 program. The paired t-test was conducted to see changes in stress, LF, HF, LF/HF, average pulse, pulse standard, and average deviation before/after experiment. The stress index was decreased in every group. Especially, the group of the 20s showed a huge decrease(24.3%), which was significant to every group except for the 50s. In the results of LF, the group of the 20s and of the 30s increased 2.8% and 4.6% respectively, which was significant. In the group of the 40s, it was increased 4.0% while it was decreased 1.2% in the 50s. Rather than women in climacteric age, it was more effective for women in younger age. In results of pulse standard, it was increased 36.5% in the 20s, 63.6% in the 40s respectively, which was significant(p<0.05, p<0.01). In the group of the 30s and 50s, the significant values were not shown although it was still increased 48.3% and 65.1% respectively. In the survey of the facial manual technique, the whole participants showed positive responses to its effects on the decrease of stress. A majority of them took easy to control feelings, and it was highly possible for them to use it perio-dically in the future. It suggested that the effects of facial manual technique could play a role on recovering psychological stability and decrease of stress. It could be considered as a safe and effective healthcare method to improve the quality of human's life.

Although salivary gland adenocarcinoma NOS accounts for third prevalence rate of all salivary gland tumors, it is one of the most aggressive solid tumors. Current therapy does not significantly improve survival rates. Thus, investigating new therapeutic modalities against salivary gland adenocarcinoma NOS is necessary. It is well known that docetaxel(TXT) as an antimicrotubulin agent induces mitotic block in proliferating cells. TXT has significant antitumor effects, and it is currently being tested in patients with malignant tumors, but TXT has not yet been tested in malignant salivary gland tumors. The purpose of this study were to examine the effects of TXT and to evaluate the biological mechanisms of TXT on salivary gland adenocarcinoma NOS. Proliferation, cell cycle regulation, connexin43 expression, apoptosis, and Fas receptor(FasR) expression were measured in cultured SGT cell line. Proliferation was little changed after 10ng/ml TXT exposure, but cellular proliferation was inhibited according to increasing concentration of TXT and time. Especially it was prominently inhibited after 96 hrs at 20ng/ml. G2-M arrest stage showed about up to 5 fold increase after exposure of TXT by flow cytometry. Apoptosis index showed about up to 8 fold increase after exposure of TXT by flow cytometry. Fas expression showed about up to 3 fold increase after exposure of TXT by flow cytometry. Apoptosis showed about up to 3 fold increase at 20ng/ml after exposure of TXT and anti-Fas agonist by flow cytometry. In Immunoslot blotting, Cx 43 protein expression was increased after TXT treatment. It suggested that TXT might induce apoptosis in SGT cells and could be used as a potent and specific chemotherapeutic tool for the treatment of salivary gland adenocarcinoma NOS in future.

Although recombinant human Bone Morphogenetic Proteins-2 (rhBMP-2) is clinically useful for bone regeneration, induction of new bone formation requires a large amount of rhBMP-2 in humans. Many investigators have been concentrated efforts on searching materials which enhance the effect of rhBMP-2, and then heparin was found as a potentiating material to rhBMP-2. The purpose of this study were histomorphometrically to analyze the enhancing effect of heparin to rhBMP-2 and to study the mode of actions of heparin to rhBMP-2. Stem cells obtained from rabbit adipose tissue were divided into 4 groups according to heparin concentrations(0, 0.25, 2.5, and 25 μg/ml) with a constant rhBMP-2 concentration(150 ng/ml) and cultured for 2, 4, and 8 days. Naphtol AS phosphate-fast blue BB staining for alkaline phosphatase content and Alizarin red staining for calcium content were performed as time schedules and the morphology and osteoblastic activity were observed closely. 5 μg/ml rhBMP-2 was mixed with the following doses of heparin: 0, 0.25, 2.5, and 25 μg/ml. Each mixture was blotted into 0.5 g of multiporous anorganic bovine bone and was inserted into the critical sized calvarial defects(diameter of 0.8-mm) of rabbits. After 1, 3, and 6 weeks, the harvested tissues were processed and stained using H&E and Masson’s trichrome methods. And the areas of newly formed bone in the grafted material were measured and statistically analyzed. During culture experiment of adipose stem cells with rhBMP-2 and heparin, the degree of osteoblastic differentiation was increased with increasing heparin concentration, but the cellular degeneration was accelerated at higher concentration of heparin as time passed. Consequently the osteoblastic differentiation of progenitor cells were accelerated as the concentration of heparin increased. In addition, the progenitor cells exhibited full differentiations early showing fast degeneration. The higher the concentration of heparin, larger newly formed bone in grafted materials was obtained in initial period. However, the increased amount of the newly formed bone in grafted materials was progressively decreased at the higher concentration of heparin as time passed. In conclusion, the heparin has influence on the osteoinductive effect of rhBMP-2 in the initial stage of bone formation. The use of heparin with rhBMP-2 could offer cheaper, safer, and improve clinical results in grafting procedures.

The carcinogenesis mechanism of human salivary gland adenocarcinoma NOS is poorly understood. MicroRNA155(miRNA155) has been involved in the carcinogenesis of many malignant tumors. The purpose of this study was to examine the role of miRNA155 in tumor growth and invasion of adenocarcinoma NOS. Using SGT cells as a model for adenocarcinoma NOS, cell proliferation was examined by MTT assay after knocking down miRNA155 expression, and cell cycle analysis was performed. Invasive capacity by a Transwell culture assay, and miRNA155 expression in SGT cell line by RT-PCR were examined. In MTT assay, proliferation of SGT-miRNA155 cells was decreased prominently after 96 hrs. Proliferation of SGT cells was markedly inhibited by knocking down miRNA155, resulting from a blockade of cell cycle in the G1 phase, but apoptosis was increased about 4 folds. In adhesion assay, SGT-miRNA155 cells decreased about 60% compared to SGT cells. In invasion assay, inhibition of miRNA155 significantly suppressed the invasive capacity of about 34% SGT cells. mRNA expression of SGT-miRNA155 cells prominently were decreased compared to SGT cells by RT-PCR. It suggested that miRNA155 could play an role in cell cycle progression and invasion in SGT cells, including antitumor effect. These results have provided insights into the carcinogenic mechanisms and new intervention method of salivary gland adenocarcinoma NOS.

Odontogenic cysts are classified into inflammatory and developmental origins. The most common representative inflammatory cyst is periapical cyst and the most common representative developmental cyst is dentigerous cyst and cyst which show character of tumor is odontogenic keratocyst and cyst of which cystic epithelial lining cells transform to ameloblastoma is unicystic ameloblastoma. Proliferation studies are needed because of various causes, different clinical and pathologic findings. Recently Ki67 has been generally used as cellular proliferation marker, which is closely related to proliferation. Because Ki67 exists in all the time of cell mitosis stage including G1, S, G2, and M, but disappear in resting phase, G0, it is widely used in the evaluation of cell and tissue proliferation activity. The purpose of this study was to establish clinical therapeutic standard through clinical prognosis associated with Ki67 protein expression because of various causes, different clinical and pathologic findings. Immunohistochemical study was performed in selected each 10 biopsy cases through LSAB reaction and HRP system using anti-Ki67 monoclonal antibody. Ki67 expression was mainly seen in the basal layer of periapical cyst, dentigerous cyst and unicystic ameloblastoma, and in suprabasal layer of odontogenic keratocyat, while positive cells appeared very low frequently in unicystic ameloblastoma. Ki67 expression was mainly observed around inflammatory area. Ki67 expression appeared to be independent on the destruction and recurrence of cystic lesion. Conclusively, high cellular proliferation could not represent destruction and recurrence degree of lesion, but this proliferation might be closely associated with circumstance such as inflammation

This study were to perform for verifying the activation areas in the human's brain during mastication by using functional-MRI (f-MRI) device on the basis of hypothesis regarding anatomical-physiological parts of brain processing the information of motor and sensory function, and to perform further more for a providing basic provisional foundation about diagnosis, treatment and prognosis of abnormal occlusion as applying functional MRI. Generally healthy 10 volunteers who have a normal occlusion were selected. The half of members of volunteers was female. Age distributions were approximately alike. Before taking a f-MRI, sufficient practice was carried out as strict standards and made volunteers be not sensible to sweet taste of gum through chewing gum for 30 minutes before taking a f-MRI. Functional images for all volunteers were firstly obtained, and then anatomical images were next. The functional images consisted of echo-planar image volumes which were sensitive to BOLD (blood oxygenation level-dependent) contrast in axial orientation. The volume covered the whole brain with a 64×64 matrix and 42 slices. Images with 64 volumes were acquired under periodic mastication. The orofacial sensorimotor cortex was primary responsible cerebral part during mastication and insula. And also supplementary motor area and cerebellum in brain were intimately connected with mastication. Other numerous anatomical parts of brain were activated in each volunteer during mastication, but there was no statistical significance in this experiment. Differences according to gender and age were no significance in this study. The f-MRI device showed the accurate and detailed image in activation area of brain through valuable device. It suggested that f-MRI might be helpful to establish the basis of funtional standard occlusion depend on activation area of brain.

Recently, oteomyelitis from oral and maxillofacial region which is an acute or chronic inflammatory process in medullary spaces or cortical surfaces of bone is uncommon in Korea. And the clinicopatholgic study of osteomyelitis in Korea has been rarely reported. The purpose of this study were to examine the clinicopatholgic analysis of osteomyelitis patients and to apply its results for treatment. Retrospective analysis of 103 cases of osteomyelitis patients treated in the Department of Oral and Maxillofacial Surgery at DKUDH from 1991 to 2000. There was a male predominance with a 2.3:1 ratio. The mean age of onset of disease was almost the same in cases of acute and chronic osteomyelitis: 29.4 years(range 1-81 years). Swelling, pain, pus discharge, and sequestration were main characteristic features of this disease entity. Acute chronic osteomyelitis of the jaws is caused mostly by a bacterial focus(odontogenic disease, periapical lesion, pericoronitis, periodontal disease, postextraction wounds, and infected fractures). It suggested that acute and chronic osteomyelitis could be basically the same disease separated by the arbitrary time limit of 1 month after onset of the disease by a true bacterial infection. And these results could play an role in the diagnosis and treatment of osteomyelitis of the jaws

New therapeutic measure are needed to improve the outcome for patients with oral squamous cell carcinoma(OSCC) because OSCC continues to portend a relatively unfavorable prognosis. Recently RNA interference(RNAi) has emerged as an effective method to target specific genes for silencing. Although overexpression of urokinase-type plasminogen activator receptor(uPAR) has been implicated in progression and metastasis of OSCC, the transfection effect of RNAi- uPAR on OSCC has been rarely reported. The purpose of this study were to examine the efficient and specific inhibition of uPAR mRNA and protein expression by siRNA targeting of uPAR through RT-PCR and immunoslot blotting, and to study cell proliferation activity, adhesion, invasion and migration in vitro compared to the controls. In MTT assay, siRNA-uPAR transfected cells showed about 70-80% cell proliferation compared to OSCC cell lines after 2 days. In adhesion assay, siRNA-uPAR transfected cells showed about 20-30% adhesion activity compared to OSCC cell lines, but similar features to those of BSA coated wells. In migration assay, siRNA-uPAR transfected cells showed about 60% migration activity compared to OSCC cell lines, but higher 3.5 folds to those of BSA coated wells. In invasion assay, siRNA-uPAR transfected cells showed about 55% invasive activity compared to parental cell lines. mRNA expression of siRNA-uPAR transfected cells showed about 10-15 % compared to parental cell lines by RT-PCR. Protein expression of siRNA-uPAR transfected cells showed about 25% compared to parental cell lines by ELISA assay. It suggested that RNAi-uPAR tranfection might be used as a potent and specific therapeutic tool for the treatment of oral squamous cell carcinoma, especially in inhibiting invasion and metastasis.

Recently, extensive research has been performed in the field of orthopedic medicine to develop cell-based therapies for the restoration of injured bone tissue. But there has been rarely reported about rehabilitaton of oral and maxillofacial bone defect using self-derived osteoblasts. Normal human osteoblast cell(NHost) was previously established into marrow-derived human mesenchymal stem cells for their capacity to proliferate and differentiate into osteoblasts under various culture conditions. The purpose of this study was to examine proliferation and differentiation of NHosts effected by growth factors with ALP activity and RT-PCR. After NHosts were cultured under basal and osteogenic medium at 37℃ and 5% CO2, they were analyzed by ALP activity and RT-PCR. BMP-2 under osteogenic medium decreased growth rate of NHosts compared to under osteogenic medium. BMP-2 under osteogenic medium induced osteoblastic differentiation in NHosts by increased ALP activity. The differentiating capacity of NHosts under osteogenic medium showed that NHosts expressed higher mRNA expression levels of OSX and OCN, while that of RUNX2 decreased after BMP-2 treatment. It suggested that NHosts having characteristics of osteoprecursor cells might be more advanced in their osteogenesis development by BMP-2, making NHosts an interesting biological tool for treatment of skeletal defects and diseases of oral and maxillofacial bone.

Odontogenic cysts are classified into inflammatory and developmental origins. The most common representative inflammatory cyst is periapical cyst and the most common representative developmental cyst is dentigerous cyst and cyst which show character of tumor is odontogenic keratocyst and cyst of which cystic epithleial lining cells transform to ameloblastoma is unicystic ameloblastoma. About ten years ago p63 protein that are closely related to p53 protein was found. Authors studied about comparative pattern of expression of p63 protein in periapical cyst, dentigerous cysts, odontogenic kertocysts and unicystic ameloblastomas. Authors selected 10 cases for every four types of cyst and performed immunohistochemical staining by using monoclonal antibody about p63 protein, LSAB(labelled streptoavidin biotin) reactant and HRP(horse raish peroxidase) system. Positive cells about p63 protein were expressed at basal layer of cystic lining epithelium in periapical cysts, odontogenic keratocysts and unicytic ameloblastomas. On the contrary, in dentigerous cysts positive cells were expressed at surfce layer. Perapical cysts and odontogenic keratocysts showed significantly high values of labelling indices.(periapical cyst:72.49%, odontogenic keratocyst:64.72%, dentigerous cyst:8.94%, unicystic ameloblastoma: 5.25%) Odontogenic keratocyst showed the most strong staining intensity and the second was periapical cyst, the third was dentigerous cyst, and lastly unicystic ameloblastoma. Conclusively cause that the positive cells appeared at surface layer in dentigerous cyst reflected the position of epithelium to the enamel, and labelling indices of p63 protein were closely related to proliferative capacity and intensity of expression closely related to the labelling index and thus labelling index was also closely related to proliferative capacity of cystic lining epithelium.

Ameloblastomas are benign odontogenic tumor and the most common neoplasm in jaws and they have locally invasive property and high recurrence rate. Four typical subtypes ameloblastomas are plexiform, follicular, granular cell and acanthomatous type, but their developmental states during tumorigenesis are uncertain. And thus authors studied about developing states of four types of ameloblastomas by immunohistochemical staining for cytokeratin 8/18 which was an intermediate filament of epithelial cell origin and for vimentin which was an intermediate filament of mesenchymal cell origin, and then by comparative analyses of the results. Authors selected seven cases for every four types of ameloblastomas, and then performed immunohistochemcial staining for cytkeratin 8/18 and vimentin to all selected specimen by using monoclonal antibodies about cytoleratin 8/18 and vimentin, LSAB(Labelled StreptoAvidin Biotin) reactant and HRP(Horse Radish Peroxidase) system. Labelling indices of cytokeratin 8/18 of plexiform and follicular types of ameloblastomas were significantly high values in the group of ameloblast-like cells and labelling indices of cytokeratin 8/18 of all types of ameloblastoma were high values in the group of transformed cells, but their differences were not significant. Labelling index of vimentin of plexiform ameloblastoma was significantly high value in the group of ameloblast-like cells and others showed comparatively lower values. Labelling index of vimentin of granular cell type of ameloblastoma in the group of transformed cells was significantly high value and others showed comparatively lower values. Consequently the most primitive form of ameloblastoma was plexiform, and more differenciated form was follicular type and granular cell type and acanthomatous type were most differenciated form of ameloblastomas

Plasminogen activator(PA) system such as urokinase plasminogen activator(uPA), urokinase PA Receptor(uPAR), tissue, tissue PA, and PA inhibitor-1&2(PAI-1&2) play a role in tumor invasion, metastasis, and proliferation. It is interested that these factors in patients with primary oral squamous cell carcinoma(Oral SCC) will be evaluated and correlated with clinicopathologic variables. Recently, these expression of primary oral SCC has been restricted to clinical or immunohistochemical study such in vivo study. The purpose of this study were to investigate the mRNA expression and cytologic concentration of uPA, uPAr, tPA, and PAI-1,2 in oral SCC cell lines compared to NHOK and to apply these results to evaluate early detection biomarkers of oral SCC in future. All the cell lines(NHOK, HN 4 and SCC 25) were cultured under KBM bullet kit at 37℃ in a 5% CO2 incubator. We studied a possible association between mRNA expression and cytosolic concentrations of uPA, uPAR, tPA, and PAI-1,2 in oral SCC cell lines compared to NHOK using RT-PCR and an enzyme-linked immunoassay(ELISA) method. uPA mRNA expression was about 5-6 folds, while uPAR was a bout 3 f olds, and PAI-1 was about 1 .5-1.6 f olds. PAI-2 was a bout1.2 -1.3 f olds t han that o f NHOK, w hile t PA w as l ower t han that of NHOK. uPA cytosolic concentrations was about 15-19 folds, while uPAR was about 8 folds, and PAI-1 was about 3-4.5 folds. PAI-2 was about 2 folds than that of NHOK, while tPA was lower than that of NHOK. Both uPA, uPAR, and PAI-1,2 cytologic concentrations were correlated with mRNA expression of oral SCC cell lines. From the aboving results, high cytosolic concentrations of uPA, uPAR, and PAI-1 & 2 were correlated with mRNA expression. It suggested that these might be specific markers for oral SCC cell lines and these results would be contributed to evaluate early detection biomarkers for human oral squamous cell carcinoma.

Plasminogen activators(PA) such as urokinase(uPA) and tissue type plasminogen activators(tPA), physiologically catalyze the conversion of the plasminogen to the wide spectrum proteinase plasmin. Because uPA and tPA are involved in cell growth, differentiation and migration of oral cancer, oral epithelial carcinogenesis including transformation of precancerous lesion into oral squamous cell carcinoma with PA is very interesting. It is important to prevent precancerous condition from transfoming into oral squamous cell carcinoma by the inhibitory effect of various drug. It is well known that cyclosporine A(CsA) as immunosuppressive properties exerts anti-cancer effects. Recently it is widely accepted that cultured immortalized oral keratinocyte (IHOK) is considered as an intermediate stage of oral carcinogenesis and used as precancerous condition in vitro. Thus it was thought that it might be interesting to investigate CsA effect on PA expression of IHOK. IHOK was cultured under KBM bullet kit at 37℃ under 95% CO2 incubator. Subconfluent IHOK cells was treated at different CsA concentration. uPA and tPA protein expression from cultured IHOK cell line has been detected by ELISA analysis in the CsA-treated samples. uPA expression of IHOK was higher than that of NHOK, while tPA was similar to that of NHOK. After CsA treatment, CsA might not effect the expression of uPA of IHOK, while showed a little effect on tPA of IHOK. It suggested that CsA had no effect in uPA expression of IHOK although uPA could be used as a marker for precancerous lesion.

The purpose of this study was to investigate the effect of cytokine-induced osteoclastogenesis on tooth movement related to orthodontic force. We evaluated the cytotoxicity as well as the expression of OPG and RANKL, which influence the homeostasis of bone metabolism. Titanium particles were applied to human periodontal ligament cells and subcultured fourth generation cells. The ALP assay and the MTT assay were used to assess changes in cytotoxicity. After 48 hours, cytotoxicity increased proportionally with the concentration of titanium. With 20 mg, the cytotoxicity was the lowest. R T-PCR was u sed for assessing m R NA l evels of O PG a nd R ANKL; after 96 hours, t he m R NAs of O PG a nd R ANKL increased steeply. A western blot analysis showed that with 20 mg of titanium, the protein expression of OPG increased linearly with time, especially a fter 96 hours, while t he p rotein e xpression o f RANKL d id n ot s how significant changes with titanium processing. Given the increase in OPG expression after the initial cytotoxicity, changes in cytotoxicity with titanium may be attributable to the antagonistic effect of OPG on cytotoxicity