본 연구는 국내 자생하는 진달래속(Rhododendron) 종들의 종자 휴면유형 분류 및 발아특성 구명을 목표로 하였다. 진달래속 종들의 배는 형태적휴면(MD)이 없는 완전히 발달된 직선(linear) 형태였으며, 만병초 및 꼬리진달래 종자는 탈리 시점에 이미 휴면이 없는 것으로 밝혀졌다. 반면에 털진달래 종자는 population 수준에서 부분적으로 생리적휴면(PD)을 가지고 있는 것으로 나타났다. 털진달래의 이러한 생리적휴면 (PD)은 외생 지베렐린(GA3) 1,000mg･L-1 처리를 통해 타파될 수 있었다. 그러나 4℃에서의 저온층적처리는 털진달래 종자 휴면 타파에 효과가 없는 것으로 나타났다. 종합적으로 판단 했을 때, 진달래속(Rhododendron) 종들의 적정 발아 환경조 건은 광조건･25/15℃(만병초), 암조건･20/10℃(꼬리진달래), 광 조건･25/15℃(털진달래)로 확인된다. 진달래속(Rhododendron) 에서의 종간 차이(interspecific variation)로 인해 모든 종이 종자 휴면유형 또는 발아특성에서 구별이 되었다. 본 연구는 국내 자생하는 진달래속(Rhododendron) 종들의 생리･생태 특성을 이해하는 데 이용될 수 있을 것이다.

This study was carried out to confirm the predatory and developmental features of N. stenoferus. We determine the host range of N. stenoferus. As a result, it was confirmed that Aphis gssypii, Myzus persicae, Planococcus citri, and Frankliniella occidentalis. N. stenoferus is thought to be able to feed on other micro pests. The test for a developmental period of N. stenoferus at 25℃ showed that the egg period was about 10 days. The nymphal period was about 18 days. Each nymphal period from 1st instar to 3rd instar nymphs was about 3 days. And the nymphal period of 4th and 5th were about 3.5 and 6 days, respectively. The female adult laid eggs in stem tissue or on leaves, and sometimes on the soaked cotton for water supply.

This study was carried out to confirm the parasitic and developmental features of A. japonica and D. suzkii was used as a parasitic natural enemy. A. japonica attacked the D. suzukii larvae and the emergence of adults were observed from D. suzukii pupae. Black spots were observed in parasitized D. suzukii larvae. Mortality of parasitized larvae, rate of parasitic and developmental feature were investigated according to developmental stages of host, D. suzukii. Mortality and rate of parasitic of D. suzukii larvae were the highest when second instar larvae were attacked. Developmental period of parasitized D. suzukii larvae showed differences to developmental stages, but there was no significant difference in developmental stage of pupal period.

An assessment is made of the anti-proliferative activity of cicada slough-derived materials against 10 human cancer cell lines, including PC-3 and DU145 prostate cancer cell lines, using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Results were compared with those of the commercially available anticancer agent with broad spectrum cisplatin. The ethanol extract of Cryptotympana spp. slough was proved to have anti-proliferative activity against A549 lung, AGS stomach, PC-3 and DU145 prostate, Hela cervix, HT-29 colon, MCF-7 breast, and SK-Hep-1 liver cancer cell lines except for Hep-2 larynx and SK-OV-3 ovary cancer cell lines. The biologically active constituent was characterized as the nonprotein α-amino acid theanine [2-amino-4-(ethylcarbamoyl)butyric acid] by spectroscopic analysis, including EI-MS and NMR. Theanine was isolated from the cicada slough as a new cytotoxic principle. Fifty percent inhibition concentration (IC50) values of the constituent against PC-3 was 6.52 μg/mL, respectively. The activity of theanine (IC50,6.52μg/mL) did not differ significantly from that of the anticancer agent cisplatin (IC50,7.39μg/mL) toward PC-3. In conclusion, further studies on the cicada slough-derived materials containing theanine as potential anticancer products or a lead molecule for the prevention or eradication from human prostate cancer.

Background : Unlike Dioscorea japonica or Dioscore opposita, Dioscore alata is a high-temperature crop mainly cultivated in Africa and subtropical regions. Due to recent climate change, its cultivation area is expanding in Korea. In 2016, the Institute for Bioresource Research clarified optimal harvesting stage to increase its tuber yield and ensure safe storability. Methods and Results : As the seed-tubers for this study, Dioscorea alata tubers were cut into a size of 50 g, disinfected with lime powder, dentated on the seed bed with electrothermal wire installed on March 20th, and then planted on the main field on April 15th. The planting distance was 120 ㎝ in width, where the furrows of 90 ㎝ were cladded with black vinyl, in two rows with a column spacing of 25 ㎝. The amounts of applied fertilizers were 2,000 ㎏ compost, 34 ㎏ N2, 28 ㎏ P2O6, and 28 ㎏ K2O. The compost and P2O5 were used in full, where as N2 and K2O were used as original fertilizer 14 (10 ㎏) and additional fertilizer 20 (18 ㎏), respectively. The crop was harvested in six times from September 20th to November 9th, in an interval of 10 days. While harvesting, the ground fresh weight yield and underground tuber yield were investigated. The underground tuber yield was 836, 1,744 and 2,975 ㎏ low at the early harvests on September 20th, 30th, and October 10th, respectively, while it was 3,622, 3,828, 3,818 ㎏ high on October 20th, 30th, and November 9th, respectively. The ground fresh weight yield remained the same. To clarify tuber storability by harvest time, they were stored at 15℃after harvest, and then healthy and corrupt tubers were studied on March 23th. The corruption ratio by harvest time was highest at 39.1% and 29.3% on October 30th and November 9th, respectively, when frost and low temperature damaged harvest. It was 59.0% and 42.8% at the early harvests on September 20th and 30th, respectively. The corruption ratio was lowest on October 20th and October 10th at 29.3% and 39.1%, respectively. Conclusion : Ford. Dioscore alata cultivation, Korea presents a premature and disadvantageous environment with lower temperature and pre-harvest frost at the time of planting. After raising seeding on an electrically heated hot bed, the optimal harvesting stage should be from planting in the middle of April where temperature rises above 1 5℃ to harvesting in the period October 10th - 20th so as to achieve the maximum tuber yield and high storability.

Background : study of long yam and short yam (Dioscorea. opposita) are cultivated in temperate regions but tropical yam (Dioscorea alata) are mainly grown in Africa and sub-tropical regions cultivated crops. Recent tropical yam cultivation area of Korea increased but lack the proper cultivation techniques to climate warming. This study was conducted to elucidate the effect of the timely harvest on Tropical yam at 2015 in Institute of Bioresources Research, GBARES. Methods and Results : Tuber of tropical yam were cut as 40 g, and dust-coating sterilized with lime. Seedlings were grown in heating wire installed seed bed from 11. March to 16. May. Experimental field were fertilized 2,000 kg compost, 34 kg N2, 28 kg P2O5, and 28 kg K2O. All amount of compost and P2O5 treated as basal fertilizer. N2 and K2O treated 14 and 10 kg of basal fertilizer, and 20 and 18 kg of additional fertilizer, respectively. Tillage, covering black PE film with 60×25 cm spacing holes on 120 cm row were conducted. Emergence days from 6. April to 18 May until 28 days to 36 days but 15. June and 9. June were each 19 and 7 days. Dry weigh in early stage due to seedling date, while seedling date according from 6. April to 18. May had no difference. seedling date was not effective on total number of tuber but number of marketable tuber (over 200 g). Marketable tuber number of seedling date according to 20. April was increased 35~132% as 4,028 number per 10 a. And tuber with 20. April was 13~73 g heavier. Tuber yield and marketable tuber yield of seedling date as affected by 20. April were respectively 2,518 and 1,273 kg per 10 a compared to 6. April and from 4. May to 15. June as 1,743~2,457 kg and 484~1,027 kg. Conclusion : Tropical yam is low temperature in the cultivation of Korea but also adverse environmental conditions. Frost is not mature enough off before harvesting. Yam had significant increased marketable tuber (over 200 g) yield due to seedling in mid-April Requires cultivation technology.

Genetically modified (GM) papaya (Carica papaya L.) line 55-1 (55-1), which is resistant to papaya ringspot virus infection, has been marketed internationally. Many countries such as the European Union, Japan, and Korea have a mandatory safety assessment, approval and labeling regulations for GM foods. Thus, there is a need for specific methods for detecting 55-1. In this study, we established a real-time PCR detection method applicable to 55-1 for a variety of papaya products. The limit of detection was possible for fresh papaya fruit up to dilutions of 0.005% and 0.01% (weight per weight [w/w]) for homozygous SunUp and heterozygous Rainbow cultivars, respectively, in non-GM papaya. The 55-1 event-specific detection method observed parallelism (r2>0.99) between the concentration of line 55-1 cultivars and Ct values obtained in amplification plots at concentrations of 0.005-10% for SunUp DNA and 0.01-10% for Rainbow DNA. The method was applicable to the qualitative detection in various types of processed products (cocktail fruit, dried fruit, juice, etc.) containing papaya as a main ingredient. Monitoring papaya products for the presence of GM papaya were demonstrated using a P35S and T-nos real-time PCR detection method but no amplification signals were detected.