본 연구에서는 액체감쇠역전회복(FLAIR) 시퀀스를 대체하였던 방법 중에 비교적 간단하면서 높은 재현성을 나타내었던 고신호 강도제거 원리를 MAGiC에 적용하여 MAGiC-FLAIR와 기존의 고속스핀에코-FLAIR 영상과 비교하여 고신호 강 도제거영상의 유용성과 임상적으로 유의미한 기준을 제시하고자 하였다. 연구방법은 MAGiC 적용 후 MAGiC-FLAIR와 MAGiC-고신호 강도제거 영상을 재구성하여, 기존의 고속스핀에코-FLAIR 영상과 각각 정성적, 정량적 평가를 비교하였 다. 정성평가결과 MAGiC-고신호 강도제거는 MAGiC-FLAIR 보다는 월등히 우수하며, 고속스핀에코-FLAIR와 유사한 결과를 보였고, 정량평가결과 MAGiC-고신호강도제거는 MAGiC-FLAIR보다 백질과 회백질 대조도는 더 우수할 뿐만 아 니라, 뇌척수액의 신호 억제도 우수한 결과를 나타냈다. Synthetic 영상을 통하여 획득한 다양한 대조도 영상 중 FLAIR의 부정확도를 고신호 강도제거기법을 적용한다면 진단적 가치를 개선하여 제공할 수 있을 것이다.

본 연구에서는 국내산 돌미나리에서 GABA생성능이 있 는 신규 유산균을 분리, 동정한 결과, Enteroccoccus casseliflavus로 확인되었다. 최근까지 Lactobacillus속과 같 은 GABA 생성 유산균에 대한 보고는 많이 되고 있고, 일 부 Enterococcs속 유산균도 보고되고 있으나 E. casseliflavus 종에 대한 보고는 없었다. 따라서 본 연구에서는 E. casseliflavus PL05 균주에 대한 GABA 생성 최적 조건을 찾기 위하여 배지의 유형, 생육 온도, 초기 pH 조건, 배양 시간, MGS 농도 및 탄소원을 포함한 다양한 조건을 테스 트하였다. PL05 균주는 MRS 혹은 TSB 배지보다 BHI 배 지에서 생육이 잘 되었으며, 배지의 초기 pH는 7-9 조건 에서 가장 생육이 왕성하였고, GABA 생성 조건 역시 유 사한 결과로 확인되었다. GABA의 기질에 해당하는 MSG 의 농도별 GABA 생성량을 조사한 결과, 7%에서 가장 높 은 생성량을 나타내었으나 5%에서도 유사한 수준으로 확 인되어 효율적인 측면에서 5%가 적합할 것으로 판단된다. 탄소원에 따른 생육 및 GABA 생성량은 말토오스를 사용 하였을 때 가장 높은 것으로 확인되었고, 이러한 최적의 조건들로 최종 테스트를 진행한 결과, 24시간째 140.06±0.71 mM의 GABA가 생성되었고, 전환율은 78.95%로 확인되 었다. 또한 반코마이신을 포함한 10개의 항생제에 대한 감 수성을 조사한 결과 내성이 없는 것을 확인하였다.

To enhance mechanical properties through improvement of dispersion stability of carbon black (CB) in epoxy resins, fluorine functional groups were introduced on the CB surface by fluorination. The changes in the chemical properties and dispersion stabilities after fluorination were evaluated with different partial pressures of fluorine gas. The mechanical properties of the fluorinated CB/epoxy composites were evaluated by the test of tensile, impact strengths and creep behavior. The fluorinated CB/epoxy composites showed approximately 1.6 and 1.1 times enhancement in the tensile and impact strengths compared to that of neat epoxy, respectively. Moreover, when a constant load was applied at 323 K, the fluorinated CB/epoxy composites lasted longer and had smaller strain changes than those of the raw CB/epoxy composites. Thus, well-dispersed CB by fluorination in epoxy resins effectively transfers mechanical stress.

The subfamily Pimplinae currently includes 1,686 worldwide species in 45 genera. 36 species in 16 genera have been recorded from South Korea. Among them, the genus Delomerista is a small sized group in the subfamily Pimplinae with 18 described species from palaearctic, nearctic, oriental regions. The South Korean Delomerista wes newly recorded for the first time from South Korea in this study. The genus Perithous is a small group in this subfamily with 17 species from palaearctic, nearctic, oriental regions. The taxonomic study of the South Korean Perithous was initiated by Kim (1955) reported one species, Perithous scurra (Panzer, 1804). The genus Xanthopimpla is a moderate size in the subfamily Pimplinae with 261 species from entire regions, except nearctic and ethiopia regions. The existing South Korean Xanthopimpla includes only one species, Xanthopimpla punctata (Fabricius, 1781). In this study, we report six unrecorded species, Delomerista mandibularis (Gravenhorst, 1829), Delomerista pfankuchi Brauns, 1905, Perithous albicinctus (Gravenhorst, 1829), Perithous townesorum (Gupta, 1982), Perithous speculator Haupt, 1954 and Xanthopimpla clavata Krieger, 1914 of subfamily Pimplinae from South Korea.

The Riptortus (stinkbug) has a specialized symbiotic organ, M4 midgut, to harboring symbiont Burkholderia. M4 midgut is located in abdomen and surrounded with insect hemolymph. Recently our group demonstrated that symbiotic Burkholderia showed different physiology after adapting in M4 gut compare with in vitro cultured Burkholderia. And population of symbiotic Burkholderia in the M4 midgut is regulated by special organ. However, the molecular mechanism to prevent spreading and migrating symbiont bacteria to other host tissues from symbiotic organ is not clear. Therefore, we assumed that symbiont Burkholderia are susceptible to host humoral immunity after established infection in M4 midgut to prevent spreading and migrating into the other host tissues through Riptortus hemolymph. To prove this assuming, we tested the susceptibility and survival rate of symbiont Burkholderia in hemolymph of Riptortus in vitro and in vivo. We also examined the susceptibility of symbiont Burkholderia using purified antimicrobial peptides (AMP), pyrrhocoricin-like, thanatin-like and defensin-like AMPs. Finally, we tested inducing ability for AMPs by systemic infection of symbiotic Burkholderia. Gene expression of purified AMPs was not different after systemic infection of both symbiont and in vitro cultured Burkholderia. Surprisingly, in vitro cultured Burkholderia resisted on bacteria injected hemolymph and purified AMPs but symbiont Burkholderia were highly susceptible in bacteria injected hemolymph and purified AMP. These results suggest that symbiont Burkholderia can't survive in the hemolymph after escaping symbiotic organ. Moreover, humoral immunity of host Riptortus is important to prevent spreading and migrating symbiont Burkholderia into the other host tissue or organ from symbiotic organ.

Isotropic pitch fibers were stabilized and carbonized for preparing carbon fibers. To optimize the duration and temperature during the stabilization process, a thermogravimetric analysis was conducted. Stabilized fibers were carbonized at 1000, 1500, and 2000℃ in a furnace under a nitrogen atmosphere. An elemental analysis confirmed that the carbon content increased with an increase in the carbonization temperature. Although short graphitic-like layers were observed with carbon fibers heat-treated at 1500 and 2000℃, Raman spectroscopy and X-ray diffraction revealed no significant effect of the carbonization temperature on the crystalline structure of the carbon fibers, indicating the limit of developing an ordered structure of isotropic pitch-based carbon fibers. The electrical conductivity of the carbonized fiber reached 3.9×10⁴S/m with the carbonization temperature increasing to 2000℃ using a four-point method.

The Diadegma fenestrale is known as parasitoid on potato tuber moth, Phthorimaea operculella and diamondback moth, Plutella xylostella. The Diadegma genus is reported to have symbiotic virus, ichnovirus, D. fenestrale Ichnovirus (DfIV) was identified from this species which is a first report. DfIV showed typical ichnovirus shape with two membranes surrounding the virus capsid. To identify DfIV genes, whole genome sequencing based on GS-FLX was conducted using purified total DfIV genomic DNA extracted from D. fenestrale calyx. About sixty ORFs were analyzed and several typical ichnovirus gene families were detected such as cys-motif, repeat element, vinnexin and vankyrin. Present study was focused on the gene expression patterns in two different lepidopteran hosts.

We investigated the molecular and kinetic properties of two acetylcholinesterases (AmAChE1 and AmAChE2) from the Western honey bee, Apis mellifera. Western blot analysis revealed that AmAChE2 has most of catalytic activity rather than AmAChE1, further suggesting that AmAChE2 is responsible for synaptic transmission in A. mellifera, in contrast to most other insects. AmAChE2 was predominately expressed in the ganglia and head containing the central nervous system (CNS), while AmAChE1 was abundantly observed not only in the CNS but also in the peripheral nervous system/non-neuronal tissues. Both AmAChEs exist as homodimers; the monomers are covalently connected via a disulfide bond under native conditions. However, AmAChE2 was associated with the cell membrane via the glycophosphatidylinositol anchor, while AmAChE1 was present as a soluble form. The two AmAChEs were functionally expressed with a baculovirus system. Kinetic analysis revealed that AmAChE2 has approximately 2,500-fold greater catalytic efficiency toward acetylthiocholine and butyrylthiocholine than AmAChE1, supporting the synaptic function of AmAChE2. In addition, AmAChE2 likely serves as the main target of the organophosphate (OP) and carbamate (CB) insecticides as judged by the lower IC50 values against AmAChE2 than against AmAChE1. When OP and CB insecticides were pre-incubated with a mixture of AmAChE1 and AmAChE2, asignificant reduction in the inhibition of AmAChE2 was observed, suggesting a protective role of AmAChE1 against xenobiotics. Taken together, based on their tissue distribution pattern, molecular and kinetic properties, AmAChE2 plays a major role in synaptic transmission, while AmAChE1 has non-neuronal functions, including chemical defense.

The Diadegma fenestrale was known as parasitoid on potato tuber moth, Phthorimaea operculella and diamondback moth, Plutella xylostella. This species, genus Diadegma are first reported from Korea. DfIV showed typical ichnovirus shape which two membranes surround virus capsids. The genome contents of DfIV consist about sixteen double-stranded DNA segments ranging 2 to 6 kb. To identify DfIV genes, whole genome sequencing based on GS-FLX was conducted using purified total viral DNA extracted from D. fenestrale calyx. About sixty ORFs were analyzed and several typical polydna virus gene family detected such as cys-motif, rep, vinnexin and vankyrin. This is the first report of DfIV and these lepidopteran host immune suppression genes will be deeply identified.

The genus Gonioctena Chevrolat is distributed in the Holarctic and Oriental regions, with more than 80 species. Many species of the genus have great similarity in external morphology and coloration, often cause misidentifications. Thus, the shape of the aedeagus is mainly used to distinguish closely related species. Fifty-four species, more than half of the known taxa, occur in northeastern Asia, including 18 species in the Russian Far East, 29 in China, 11 in Korea, and 18 in Japan. Among these, G. gracilicornis Kraatz (type locality: Amur) is widely distributed in Russia, China, Mongolia and Korea. However, extensive investigation on Gonioctena in Korea revealed that the Korean population of G. gracilicornis differs from that of the Russian by the coloration, the puncturation of pronotum, and the shape of aedeagus. The purpose of this study is to describe a new species of Gonioctena that was previously misidentified as G. gracilicornis. A key to Korean Gonioctena, distribution, and host plant are also provided.

Early life stage mortality in fish is one of the problems faced by loach aquaculture. However, our understanding of immune system in early life stage fish is still incomplete, and the information available is restricted to a few fish species. In the present work, we investigated the expression of immune-related transcripts in loach during early development. In fishes, recombination-activating gene 1 (RAG-1) and sacsin (SACS) have been considered as immunological function. In this study, the expression of the both genes was assessed throughout the early developmental stages of loach using real-time PCR method. maRAG-1 mRNA was first detected in 0 dph, observed the increased mostly until 40 dph. Significant expression of maRAG-1 was detected in 0 to 40 dph. These patterns of expression may suggest that the loach start to develop its function after hatching. On the other hand, maSACS was detected in unfertilized oocyte to molura stages and 0 to 40 dph. maSACS mRNA transcripts were detected in unfertilized oocytes, suggesting that they are maternally transferred.

Neutropenic enterocolitis (NE), the most serious gastrointestinal complication, has been reported as a clinical syndrome that occurs in the setting of disease- or chemotherapy-induced neutropenia. Complications of NE include bowel necrosis with perforation, fistula, stenosis, massive bleeding, abscess formation, and pneumatosis intestinalis (PI). Most physicians recommend initial conservative management with bowel rest, intravenous fluids, total parenteral nutrition, broad-spectrum antibiotics, and normalization of neutrophil counts. Surgical intervention is recommended in the event of obstruction, perforation, persistent gastrointestinal bleeding despite correction of thrombocytopenia and coagulopathy, or clinical deterioration. We experienced a patient whose abdominal computed tomography scan showed pneumoretroperitoneum, intramural gas in the colon, and inferior vena caval gas. Her condition improved after treatment with granulocyte colony-stimulating factor and broad-spectrum antibiotics. We report on this case along with a review of the literature.