In the present study a two-mode, separately concurring resonant cavity model is proposed for theoretical interpretation of the 3 minute umbral oscillation. The proposed model has been investigated by calculating the transmission coefficients of the waves propagating through the umbral photosphere (photospheric weak-field cavity) and chromosphere (chromospheric strong-field cavity) into the corona, for 3 different umbral model atmospheres by Staude (1982), Beebe et al. (1982) and Avrett (1981). In computing the transmission coefficients we made use of multi-layer approximation by representing the umbra] atmosphere by a number of separate layers with (1) temperature varying linearly with depth and (2) temperature constant within each layer. The medium is assumed to be compressible, non-viscous, perfectly conducting under gravity. The computed resonant periods, transmission spectra, phase spectra, and kinetic energy density of the waves associated with the oscillations are presented in comparison with the observations and their model dependent characteristics are discussed.

A Hosta cultivar ‘Neulpureum 1’ was bred at the Korea National Arboretum, which produces new cultivars using vegetative propagation techniques. The new cultivar ‘Neulpureum 1’ was derived by crossing Hosta minor with Hosta ‘Krossa Regal’. Among the induced leaf-color- and shape-modified hosta plants, the plants that exhibited deep-green color and small-bended leaves were selected. ‘Neulpureum 1’ maintained the deep-green leaves for longer than Hosta ‘Krossa Regal’. Additionally, the plant height of ‘Neulpureum 1’ was shorter than that of Hosta ‘Krossa Regal’ and several leaves were observed on the new cultivar; therefore, it is likely to be used as a pot plant. The botanical characteristics were investigated for three years beginning 2012. A Hosta ‘Neulpureum 1’ can prove to be useful as a material for a pot plant or as ground cover plant at half-shadow place.

Tiarella polyphylla D. Don is a native plant distributed only in Ulleung Island in Korea and has been traditionally used for medicinal purposes, although it is also used ornamentally. This study was conducted to determine the requirements for dormancy break and germination and to classify the type of seed dormancy. The experiments were performed with cold stratification (0 or 12 weeks at 5℃), warm stratification (0, 4, 8, or 12 weeks at 23℃, followed by 8 weeks at 5℃, and then incubation at 23℃), and GA3 treatments (0, 10, 100, or 1000 ㎎/L). The treated seeds were incubated on aseptic media at room chamber (23℃, a 16h photoperiod of fluorescent lamps with 40 μmol ․ m-2 ․ s-1). The seeds were dispersed in nature as underdeveloped embryos with no physical barrier to absorb water to prevent water absorption. However, the seeds did not germinate for 30 days after sowing without any pre-treatments. Thus, the seeds had morphological dormancy (MD) and physiological dormancy (PD). The final germination percentage following cold stratification (0 or 12 weeks) was 66.7% and 45.6%, respectively. The cold stratification delayed seed germination by about 3 weeks. In the warm stratification experiment (0, 4, 8, or 12 weeks), the final germination percentage was 21.1%, 27.8%, 41.1%, and 57.8%, respectively, 20 weeks after sowing. The embryos of the T. polyphylla seed grew in relatively warm temperatures (23℃). GA3 application overcame seed dormancy and promoted germination. Following GA3 treatment (0, 10, 100, or 1000 ㎎/L), the final germination percentage was 33.3%, 45.0%, 42.5%, and 72.5%, respectively. These results suggest that the T. polyphylla seeds had non-deep simple morphophysiological dormancy (MPD) and GA3 treatment could be used as a substitute for warm stratification for breaking seed dormancy. To our knowledge, this is the first report of seed dormancy characteristics of the genus Tiarella native to Korea.

본 연구에서는 VFSMOD-W 모형을 활용하여 새만금 유역의 초생대 기법 적용에 따른 유사저감효과를 예측하고자 하였다. 먼저 모형의 신뢰도 확보를 위해 새만금 유역 내 초생대 실측치를 활용하여 매개변수를 보정하였으며, 새만금 유역 내 밭경지 특성을 파악하여 이를 토대로 모의 시나리오를 개발하였다. 다양한 밭경지 특성을 반영하기 위해 개발된 모의시나리오는 밭경지 규모 1 ha, 5 ha, 10 ha로 밭의 폭과 길이 비는 1 : 1를 적용 하였으며, 밭의 경사는 7%, 15%를 고려하였다. 또한 강우조건은 50 mm, 100 mm, 150 mm, 200 mm 일강우량을 적용하였다. 그 결과, 초생대의 유출저감효과는 밭경사 7%와 15%에 대해 2.9~13.5%, 2.9~12.1%로 각각 나타났으며, 유사저감효과는 33.8~97.0%, 27.1~85.9%로 각각 나타나 유출저감효과에 비해 상대적으로 높은 저감효과를 나타내었다. 유출 및 유사 발생에 영향을 미치는 밭규모, 경사 및 강우량 인자에 따라 초생대 효과는 큰 차이를 나타내었다. 본 모의 결과를 바탕으로 새만금 유역내 밭경지 규모 10 ha 미만, 경사 15% 이내의 조건에서 밭면적 10% 규모로 초생대를 조성할 경우 유사저감 효과를 기대할 수 있을 것으로 사료된다.

Hyperlipidemia is an increase in one or more of the plasma lipids, including triglycerides, cholesterol. Ginseng has been used as a valuable tonic and for the treatment of various diseases. The objective of this study was to evaluate the effects of Jinan red ginseng (JRG) water extract on the blood and serum in rabbits with hyperlipidemia induced by poloxamer 407 when supplied in drinking water. JRG treatment was performed for 20 weeks. We evaluated the effects of the JRG treatment on diabetes through hematological and biochemical analysis before and after JRG treatment were performed. Our results indicate that LDL, total cholesterol, and triglycerides were significantly decreased compared prior JRG supply. CRE, BUN, CK and UA levels indicating renal functions are significantly reduced when compared to those prior to the JRG supply. In addition, AST, ALT, ALP, and LDH were significantly reduced indicating hepatoprotective effect. Blood electrolytes deteriorated in HL rabbits were improved when JRG supplied. In conclusion, Biochemical and hematological analysis demonstrate that the JRG is effective to alleviate the hyperlipidemia signs.

As an aquaculture effluent is recognized as a source of water pollution, many methods are being researched to solve this problem. It has been reported that the aquaculture effluent contains many organic compounds containing nitrogen, phosphorus, potassium, and calcium. The purpose of this study was to investigate the possibility of using the effluent from bio-floc technology (BFT) inland aquaculture for fertilizing blueberry ‘Duke’ to promote the growth. The experiment was conducted in a commercial blueberry farm in Jinju, Gyeongsangnam-do, where blueberries are actually cultivated in order to find out the effects of fertilizers in situ. The experiment was carried out with five treatments: control with only irrigation, conventional nutrient solution fertigation, and the fertigation with three concentrations (×1, ×0.5, or ×0.25) of effluent from BFT (Bio-Floc Technology) inland aquaculture. The treatment period was from the beginning of April, when a new leaf of blueberry began to develop, to the middle of June, when the blueberry was harvested, for a total of 10 weeks. As a result of the experiment, the treatments with effluent from BFT inland aquaculture showed better growth qualities than those of control and conventional cultivation. However, there was no statistically significant difference among all treatments regarding the total fruit production. Based on these results, it was concluded that the effluent from BFT inland aquaculture can be used for the cultivation of blueberry because it is equivalent to conventional cultivation method. This study provided a new viewpoint of recycling the effluent from BFT for agricultural purpose to reduce water pollution problems due to aquaculture wastewater.

장 건강에 유익한 프리바이오틱스 소재를 개발하기 위하여 배변을 촉진하는 효능을 지닌 붉은팥을 식품 발효에 이용되는 Bacillus subtilis KCCM 11965P로 발효하여 다음과 같은 결과를 얻었다. 붉은팥의 일반성분은 회분 3.35±0.04%, 조단백질 21.1±0.19%, 조지방 0.35±0.02% 함 유되었다. 붉은팥 원물 1%, 3%, 5%와 Bacillus subtilis KCCM 11965P 3% (v/v)를 접종하여 0, 24, 48, 72시간 배양 하였다. 배양액의 총균수를 측정한 결과 붉은팥 원물을 3% 첨가한 후 72시간 배양군에서 Bacillus subtilis KCCM 11965P 발효가 가장 적합하였다. 발효 시간에 증가함에 따라 총 폴리페놀 함량과 DPPH 라디칼 소거활성이 증가하였다. Protease 활성은 붉은팥 원물 5% 첨가한 후 72시간 배양한 군(2.69±0.003 unit/mL)에서 활성이 가장 높았다. 발효시간과 붉은팥 원물 첨가 농도가 증가함에 따라 α -amylase 활성도 증가하였으며, 붉은팥 원물 5% 첨가한 후 72시간 배양한 군에서 0시간 배양군(1.0±0.1 unit/mL) 보다 26.0±0.2 unit/mL로 증가하였다. Bacillus subtilis KCCM 11965P로 72시간 배양한 후 유리아미노산을 측정한 결과 leucine은 붉은팥 원물 5% 첨가한 0시간 배양군 5.22 mg/L 에서 67.59 mg/L로 증가하였으며, 비필수아미노산인 tyrosine은 5% 첨가 0시간 배양군 10.08 mg/L에서 259.35 mg/L로 증가하였다. 이와 같이 Bacillus subtilis KCCM 11965P로 붉은팥을 발효하면 항산화 활성, protease 효소활성, 및 α-amylase 효소 활성이 증가하였으며, 유리아미노 산과 유기산이 증가하였다. 붉은팥을 발효하는데 Bacillus subtilis KCCM 11965P가 적합할 것으로 판단되며, 붉은팥은 프로바이오틱스를 활성화시켜 장 건강을 증진시킬 수 있는 프리바이오틱스 소재로 개발할 수 있는 가능성을 시사 하였다.

As the interest in urban agriculture and people’s demand for eco-friendly food grow, the number of urban gardeners who directly grow and harvest will increase. The aim of this study was to compare the effect of conventional fertilizer with the effluent water from the biofloc technology inland aquaculture in the concept of resource circulation as a substitute for fertilizer used in urban agriculture. In order to verify the effect of fertilizer on the cultivation of cherry tomato, tomato seedlings were transplanted on the horticultural soil without nutrients and treated for 8 weeks. Experimental treatment was carried out in five treatments: control, hyponex solution (HS), slow-released fertilizer (SF), effluent water daily treatment (DE), and effluent water treatment once a week (WE). The growth characteristics of the cherry tomatoes showed significant differences among the treatments except for root length. In the case of plant length was shown HS=WE›DE=SF›control, and stem diameter was shown DE=SF ›WE=HS›control. It was found that HS and WE were not significantly different in shoot length and diameter, and DE and SF showed no difference. In the case of number of leaves, HS, DE, and WE showed a significantly higher level than SF and control. In other growth characteristics, DE and SF showed higher growth. As for the growth of cherry tomato fruit, the sugar content did not show any significant difference among the treatments except control. Weight per fruit and yield were significantly higher in HS and WE than DE and SF. Based on these results, it is considered that the use of effluent water is comparable to the use of urban agricultural fertilizer as compared with chemical fertilizers in cultivating crops.

최근 내수면양식장의 BFT 양식 배출수의 도시농업용 활용에 관한 연구가 활발하게 본 연구진에 의해 진행되어지고 있다. 하지만 도시농 업용으로 한정된 연구에서 비료가 다량으로 사용되어 지고 있는 과수에의 활용에 대한 연구 필요성이 대두되어 누구나 가정에서도 재배가 가능한 블루베리에서 BFT 배출수의 효과를 구명하여 보고자 본 연구를 수행하였다. BFT 배출수는 내수면양식연구센터 틸라피아를 양식 하고 있는 양식조의 물을 채취하여 무처리, 농장 관행법, 배출수 원액, 배출수 1/2희석액, 배출수 1/4 희석액 시비 등 5처리로 실험하여 다음 과 같은 결론을 얻었다. 블루베리의 총생산량과 과립당 무게는 처리간 유의성이 없어 배출수가 기존의 비료를 대체하여도 블루베리 열매 의 생육에 영향을 주지 않아 비료로서의 이용가능성이 있었음을 확인하였다. 그리고 과실내 무기성분의 변화에서는 나트륨과 철의 함량이 높게 나와 이를 줄일 수 있는 배출수 비료화의 또 다른 연구 점을 보여주고 있다. 블루베리의 고유 기능성 물질인 안토시아닌의 함량 결과에 서는 처리에 따른 일관성을 보이고 있지 않아 기능성물질의 변화에도 배출수의 처리가 나쁜 영향을 보이지 않음을 알 수 있어 블루베리의 재배에 있어서도 배출수의 비료활용도는 가능성이 있다는 결론을 얻을 수 있었다. 하지만 비료로서의 사용가능성을 더 높이기 위해서는 보다 정제된 배출수의 사용과 효과를 얻기 위해서는 배출수의 가공적 연구가 더욱 필요하다고 판단된다.

본 연구는 국제적으로 해양투기의 기준이 엄격해지고 있는 현재의 실정에 맞추어 양식장에서 배출되는 배출수를 자원의 선순환의 개념으로 이를 토마토 재배에 적용하여 도시농업용 비료로서의 이용가치를 구명하고자 수행하였다. 시험용 작물로는 완숙형 토마토‘슈퍼 선로드’를 사용하였으며, BFT 배출수는 내수면양식연구센터 틸라피아를 양식하고 있는 양식조의 물을 채취하여 무처리, 상업용 하이포넥스500배 희 석액, 배출수 원액을 매일 관주, 배출수 원액을 1주에 1회 관주, 완효성 고체비료 시비 등 5처리로 실험하여 다음과 같은 결론을 얻었다. 배출 수원액을 매일 처리하는 구가 T/R율과 엽록소함량이 제일 높은 결과를 보였으며, 과실의 당도도 제일 높은 결과를 보였다. 또한 토마토의 기 능성 물질은 리코펜함량의 결과에서도 하이포넥스와 관행의 재배방법보다 3배가 많은 함량의 결과를 보였다. 하지만 토마토 체내의 무기성 분의 함량결과 질소가 다른 처리구에 비해 제일 많은 결과를 보여 비료사용으로서의 보완점을 연구해야하는 문제점도 보였다. 하지만 생육 과 기능성 물질의 함량결과등을 고려하여 본다면 BFT 양식 배출수의 비료효과에 대한 가능성은 매우 높은 것으로 나타났다.

Background : Licorice has been used as a source of medicine and a food material in East-Asia. Recently, demand for licorice increased in market due to a growing interest in health. Thus we conducted breeding research to solve the problems associated with domestically cultivated licorice such as low productivity and low glycyrrhizin content. Methods and Results : We crossed European licorice (G. glabra L.; female parent) and Chinese licorice (G. uralensis Fisch; male parent) in the greenhouse in May 2007. In September 2007, crossed and germinated seeds were retrieved and sown in the greenhouse. In June 2008, stolons were separated from the F1 licorice seedlings and cultivated, resulting in 32 clonal lines of interspecific hybrids. Among them we selected good lines and then conducted the replicated yield trials (RYT) in 2012-2013 and local adaptability test (LAT) in 2014-2015. The results, GLYES9 showed that was elect of stem, oblong of leaf shape, red-brown of root color. Glycyrrhizin conten of GLYES9 (3.0%) was higher than G. uralensis (1.9%) at four regions from 2014 to 2015. GLYES9 was less than 10% in the desease of brown spot (G. uralensis was more than 30%). The root yield of GLYES9 was 4.31 ton per hectare, which was increased 193% compared with a check variety of G. uralensis. Therfore, we named GLYES9 as new cultivar ‘Dagam’. Conclusion : Depending on the above results, we have developed a new licorice cultivar ‘Dagam’ by the medicinal crop breeding team of National Institute of Horticulture and Herbal Science, RDA, in 2015. It showed brown spot disease resistant, high-glycyrrhizn content and high-yielding than colleted Glycyrrhiza spp.

Background : Cucuma longa L., in the family Zingiberaceae, is distributed in tropical and/or sub-tropical regions mainly in India and China. This species is commonly called tumeric, powder is used as medicinal herbs and/or flavor enhancer. It has been cultivated in southern region mainly Jindo. However, it might be possible to extend cultivation region due to rise in average temperature. In order to select superior lines, agronomic characteristics is commonly used. Because this is not the ultimate solution, the DNA marker approach has benefited the modern plant breeding. Therefore an easy approach by using one kind of primer have been developed from random amplification of polymorphic DNA sequences (RAPD) to discriminate effectively between different cultivars of Cucuma species Methods and Results : DNAs were extracted from the harvested roots of Cucuma sp. using DNeasy plant Mini kit (Qiagen, Hilen, Germany). These plants cultivated from GARES (Hamyang) and used for PCR amplification. The relative concentration of the extracted DNA was estimated Nano Drop ND-1000 (NanoDrop Technologies, Wklmington, De, USA) and final DNA concentration was adjusted to 5.5 ng/㎕. In this study 9 primer pairs were tested on 8 Cucuma sp. These primers showed polymorphism in Cucuma sp. The cluster dendogram showed that the similarity coefficients ranged from 0.68 to 0.87, CUR02 turned out to be CUR11, and CUR16 is similar to CUR17. Conclusion : These finding could be used for further research on cultivar development by using molecular breeding techniques and for conservation of the genetic diversity of Cucuma species. These data on polymorphism difference based on RAPD will be give us invaluable breeding information by selection of superior lines.

This report describes the sex differentiation of the Korean rose bitterling, Rhodeus uyekii, from hatching to 170 days post-hatch (DPH) in relation to total length (TL), body weight (BW), and integral water temperature (IWT). The growth curve of TL from just hatching to 83 DPH was 5.144e0.045t (R² = 0.961; t, time), and that of BW was 2.398e0.086t (R² = 0.725). Primordial germ cells (PGCs) were observed at 17 DPH (7.9 mm TL, 3.74 mg BW, 374°C IWT), and thereafter began to protrude into the peritoneal cavity. At 21 DPH (9.2±0.14 mm TL, 4.8±0.07 mg BW, 462°C IWT), some PGCs contained condensed chromatin and oocyte were observed in meiotic prophase. In contrast to the ovaries, which grew gradually after sexual differentiation, testes began multiplying at 25 DPH (10.1 mm TL, 5.42 mg BW, 550°C IWT), when testicular differentiation was first identified, and multiplied continuously thereafter. At 33 DPH (11.2 mm TL, 10.5 mg BW, 726°C IWT), the developing testes contained spermatogonia that exhibited mitotic activity. No spermatocyte or sperm cell was observed until 83 DPH (18.9 TL, 48.2 mg BW, 1,826°C IWT). At 170 DPH (32.5 mm TL, 270.1 mg BW, 3,740°C IWT), which was the end point of this study, the mature ovaries showed germinal vesicle breakdown, while the mature testes contained observable spermatocytes and sperm cells. These results allow us to identify the sex differentiation type of the Korean rose bitterling as differentiated gonochoristic.

Background : This study was carried out to survey the effect of IBA (indole-3-butric acid) concentration on root development characteristics in Viburnum dilatatum cuttings. Methods and Results : The cuttings were collected on July 28, 2016 from plants growing in Korea National Arboretum. Cuttings were treated with different IBA concentrations [0 (control), 10, 100, 1000, 2000 ㎎⋅L-¹] for 10 seconds. Rooting parameters (root number, the longest root length, root fresh weight, root dry weight, rooting percentage, and survival rate) were observed at 8 weeks after cuttings. Rooting percentage was 43, 33, 17, 47, and 40 % at 0, 10, 100, 1000, and 2000 ㎎⋅L-¹ of IBA, respectively. Root number (13.4) was highest under 1000 ㎎⋅L-¹ IBA. Root length (9.4 ㎝) and Root weight (fresh and dry) were highest under 1000 ㎎⋅L-¹ IBA. Conclusion : According to the results, 1000 ㎎⋅L-¹ IBA was the best treatment for increasing percentage rooting percentage and other root development characteristics of V. dilatatum cuttings.

Background : Clematis trichotoma is a deciduous climber belonging to the family Ranunculaceae. This study was carried out to survey the effect of shading levels on growth characteristics in Clematis trichotoma seedling. Methods and Results : Seeds were collected from plants growing in the Mt. Kariwang Jeongseon, Gangwon-do in October 2013, and they were sown to 96 cell plug tray filled with Peatmoss (TKS-2) soil at March, 2015. The experiment was performed with four different shading levels (0, 30, 60, 90%) at July, 2015. According to the experiment, plant height was the highest under 90% of shading. It was found that fresh weight and dry weight of clematis trichotoma were the highest under 90% of shading. The leaf number was the highest under 30% of shading. The leaf number decreased as the shading level increased. Root number and length were the highest under 90% of shading. Conclusion : According to the results, Clematis trichotoma seedling showed the highest growth under 90% shading.