Rhodopseudomonas capsulata H161에 다량 축적되어 있는, 천연 색소 자원으로 중요한 carotenoid 색소 생성조건을 검토하기 위하여 본 실험을 행하였다. Rhodopseudomonas capsulata H161이 생성하는 주된 carotenoid는 spheroidene, rhodovibrin, spirilloxanthin, lycopene이었다. 본 균주는 anaerobic light, 온도 30℃, pH 7.4, 광도 5, 000Lux에서 5일간 배양하였을 때 균체 생성과 carotenoid 생성량이 최대치를 보여주었다.

흰쥐로부터 뇌, 심장, 관장, 폐, 신장 및 흉선 등 12종류의 기관을 계통별로 해부하여 homogenates를 만들고 cytosol을 분획한 다음, 이것을 효소원으로 하여 inosltol phosphates 대사회로에서 핵심효소인 PLC, IP_3K 및 Ins(1, 4, 5)P_3 5-phosphatase의 활성도를 조직별로 비교 측정하였다. 흰쥐의 뇌에서 분리 정제된 53KDa의 IP_3K를 Balb/c 마우스에 면역 후 비장 세포를 얻어서 골수암세포인 myeloma cells (SP_2/Ag 0-14)에 세포융합, 스크리닝 및 클로닝과정을 거쳐서 anti IP_3K murine monoclonal antibodies를 만들었다. 이 과정에서 18개의 hybridoma clone이 얻어졌고 그 중 10개의 clone만이 nonspecific binding에 기인되는background가 낮았다. Ascitic fluid를 생산시킨 후 그 Ab를 Affi-gel 15로 정제하여 IgG의 subtype을 결정하였다. 이 항체들 중에서 IgG_1과 IgG_2b를 함께 사용하여 조직의 IP_3K에 대한 immuno 반응성을 검증한 결과 대체로 비슷한 비경쟁적 저해를 보였으며 뇌조직의 IP_3K가 예민한 반응을나타내었고, 심장조직에서는 현저히 activity가 낮았다. 흰쥐의 뇌로부터 16, 100배로 정제된IP_3K효소를 사용하여 얻어진 Km값은 1.8mM, 최대반응속도V_max 값은 5.41μ㏖/mln/㎖이었다. Western blot 결과 심장조직에서는 40KDa의 IP_3K만이 관찰되었으며, 뇌속에는 면역학적으로 서로 다른 3가지의 IP_3K(53, 51 및 40KDa)가 존재하였다. 그 중 53KDa의 단백질이 활성이 큰 주 효소이며, 1.8Kb의 IP_3K gene을 완전히 encoding하는 ^32P-labeled cDNA를 probe로 만들어 Northern blot법으로 IP_3K의 mRNA를 정량한 결과 성장단계별로 이들은 모두 transcriptional 수준으로 조절받고 있음을 밝혔다.

刈取가 알팔파根瘤의 着生 및 發達과 室素固定活性에 미치는 影響을 檢討하기 위하여 圃場實驗에서는 刈取區와 非刈取區로 구분하여 時期別로 Acetylene還元法에 의하여 根瘤의 室素固定活性을 測定하였다. 養液栽培實驗에서는 地上部의 50%와 90%刈取, 花芽의 除法, 根瘤의 50%와 100% 除法등을 組合處理後 알팔파 再生과 根瘤의 着生과 發達에 미치는 影響을 檢討하였다. 1. 圃場實驗에서 根瘤의 무게는 1回刈取後 30%, 2 回刈取後 25%의 減少가 있었고

Background: Recently, jujube (Zizyphus jujuba Mill.) has been attracting attention as a fruit, and its cultivation in rain shelter house is increasing to produce the high quality fresh jujube. This study was carried out to investigate the growth and fruit characteristics of jujube according to the types of rain shelter house. Methods and Results: The characteristics of 5-year-old Bokjo cultivar cultivated in 3 types of rain shelter house, multi span rain shelter house with roof vent (Type I), single span house with a column in the center and roof vent (Type II) and single span house with a column in the center and without roof vent (Type III), and open field were examined. The sprouting and blooming period were different among the types of rain shelter house. The diameter of main stem was higher in rain shelter houses than in the open field. There was no a significant difference in fruit number per leaf stem among the types of cultivation. The incidence of fruit cracking in open field cultivation which was 51.2% was much higher than that in Type I 21.6%, Type II 19.3%, and Type III 25.5%. The fruit size and weight in rain shelter houses, especially in Type III rain shelter house were higher than those in the open field and the soluble solids content of fruit in Type I and Type II was higher than in Type III rain shelter house and the open field. Conclusions: The results show that the growth and fruit quality of jujube were improved by cultivation in rain shelter house, and affected by the types of rain shelter house.

Background : The rhizome of ginger (Zingiber officinale) is widely used as a spice or a traditional medicine. It contains the phenolic compounds, such as gingerol, zingerone and shogaols as the major pungent compound. Ginger is commonly used for various types of "stomach problems," including motion sickness, colic, upset stomach, gas, diarrhea, irritable bowel syndrome (IBS), nausea. The cultivation area of ginger is 1,046 ha, and it is cultivated as 5th of 55 medicinal crops in Korea (2016). When ginger is cultivated in a vinyl house, the growing periods can be prolonged by early sowing and the yield is improved compared to the conventional cultivation. This experiment was carried out to investigated on growth, rhizome shape and yield as affected by the soil covering in rain-shelter greenhouse. Methods and Results : Seed ginger was planted on March 10 at 75 × 15 ㎝ planting density in greenhouse. The soil covering on furrow area was carried out on June 20, July 20 and August 20. The control, conventional cultivation was planted four rows on 120 ㎝ wide ridge. As a results of applying the soil covering, emergence was 2 - 3 days earlier than that of conventional cultivation. On November 5, samples were harvested for each treatment, and growth characteristics, tuber shape, and yield were investigated. As the number of soil-cover increased up to 3-times, the weight of the tuber increased to 44.4% and the length to 21.9% as compared with the control. However, as the number of soil-cover increased, workspace was needed, resulting in fewer seeding gingers and lower total yield. Therefore, it was advantageous to perform two times soil-cover to obtain the maximum yield. In the case of soil cultivation, the length of the foot was longer than that of conventional cultivation, and the quality was improved. Conclusion : From the above results, it is recommended that the soil covering is carried out twice at greenhouse’s cultivation. The first covering is in mid-June, and the second is in mid-July.

Background : Zingiber officinale is known as the source of the pungent, aromatic spice called ginger. Ginger, especially dried rhizomes, has been widely used for many medicinal purposes. The pungent principles in ginger are the non-volatile phenolic compounds gingerol, gingeridione and shogaol. It is cultivated 1,046 ha as 5 th of 55 medicinal crops in Korea (2016). Ginger has been successfully propagated using internodal cuttings. Ginger emergences at 18℃ or higher, and it takes about 40 - 50 days to emerges after sowing. Therefore, it is required a cultivation method that promote the emergence. Methods and Results : The internodal cuttings with 25 – 35 g was planted on 30 April at 30 × 30 ㎝ on 120 ㎝ broad bed. Five covering materials included the illite non-woven fabric (18 g/㎡), rice straw and vinyl were treated to promote emergence and early growth. Soil temperature covered by illite non-woven fabric was higher by 2.6 - 2.8℃ than that of rice straw covering, and lower by 2.2 - 2.4℃ than that of the transparent vinyl mulching. Emergence was faster in the illite non-woven fabric mulching by 5 - 6 days compared to vinyl mulching. It was presumed to be due to the small diurnal difference with increasing soil temperature. The covering with illite non-woven fabric had a large plant height and a large leaf number. So, the early growth in the covering of illite non-woven fabric was better than other treatments. There was a differences in rhizome’s yield among treatments. The content of gingerol was 2.29 – 2.37 ㎎/g and there was no significant difference. Conclusion : From the above results, covering illite non-woven fabric until sprouting after planting promoted its emergence and early growth.

본 연구는 나리 주요 품종의 종간교잡에서 생기는 삭과의 배 유 퇴화방지와 미숙배 형성 시 발생하는 이상증상을 경감하기 위해 나리 종간교잡계통의 자방배양 했을 때 cytokinin 첨가가 신초형성에 미치는 영향을 구명하고자 하였다. FO계통은 BA 2.0 ㎎/L에서 신초 발생율이 18%로 가장 효과적이었고, BA 3.0 ㎎/L와 5.0 ㎎/L에서는 12%로 다소 저조한 결과였다. TDZ 첨가 의 경우는 대조구와 같거나 비슷한 경향이었다. FA계통은 TDZ 첨가 시 대체적으로 신초형성율이 높았고 0.3 ㎎/L 처리가 효과 적이었다. 반면 BA를 첨가한 배지에서는 14.3%에서 15.7%로 대 조구와 큰 차이가 없었다. FLA계통에서 신초발생율은 생장조 절제에 의해 증가되었으나 대조구 20%와 비교했을 때 BA 20~ 24%, TDZ 22~24%로 처리효과는 미미하였다.

The main nutritional problem of sorghumxsudangrass hybrid (Sorghum bicolorxSorghum sudanese) silage is low quality and palatability. This experiment was conducted to evaluate whether organic com grain and crushed rice addition of sorghumxsudangrass hybrid silage increases forage quality of the silage. The sorghumxsudangrass hybrid silages with added com grain and crushed rice were similar to conventional corn silage in moisture content. However organic sorghumxsudangrass hybrid silage had low pH values. Silage added with gain and byproduct had higher crude ash, acid detergent fiber (ADF) and neutral detergent fiber (NDF) contents than control corn silages, while its non-fiber carbohydrate (NFC) and total digestible nutrients (TDN) showed the opposite results. Lactic acid, butyric acid and lactic percentage oftotal organic acid (UT) of sorghumxsudangrass hybrid silages were higher than those of com silage, but acetic acid was higher than control. In vitro dry matter digestibi/ity (IVDMD) of com silage was higher than other sorghum silages. Feed intake of sorghumxsudangrass hybrid silage added with crushed rice was highest among silages. Therefore, these data indicate that crushed rice and com grain added sorghumxsudangrass hybrid silage could be recommended as the most effective treatment for increasing silage quality and palatability.