Clostridium perfringens (C. perfringens) may cause diarrhea and enterotoxemia in adult and young livestock, leading to problems in the production and management of farms. Four hundred fecal samples were collected from 25 goat farms located in Gyeongsangbuk-do Province in the Republic of Korea. Sixteen C. perfringens strains were isolates from fecal samples, and the isolates were identified as type A (n=11) and type D (n=5). Additionally, α- and ε-toxin genes were detected in 16 and 5 strains by PCR, respectively, and the enterotoxin gene was presented in 2 strains. The antibiotic susceptibility test was performed using the disk diffusion method and E-test method. In the disk diffusion method, ampicillin (n=16) and chloramphenicol (n=15) were highly susceptible to 16 C. perfringens isolates. In the E-test method, ampicillin, amoxicillin, amoxicillin/clavulanic acid and meropenem were susceptible to more than 14 of 16 C. perfringens isolates. This study indicates that administration of antibiotics such as ampicillin, amoxicillin/clavulanic acid and meropenem can prevent and treat C. perfringens infections in goats.

후각 신경 시스템은 일반 생활 환경에서 많은 다양한 화학 물질을 인식하고 구별한다. 곤충에서는 다양한 화학 물질을 기호적 또는 회피적인 특이성을 부여하고 이를 구분해 낼 수 있는 고도로 발달된 후각신경 수용체들로 구성된 냄새 맡는 (odorant-gated) 이온 채널 군을 진화시켰다. 최근에 후각 수용체와 단백질을 포함한 olfaction 관련한 진딧물 게놈 밝혀졌고, 초파리에서 다양하게 분화되어 있는 후각 수용체들이 보고되고 있다. 후각 신경 수용체의 유전체는 매우 높은 보전적인 염기 서열을 가지고 있으며, 체계적인 신호 전달 시스템을 갖추고 있다. 대표적 수용체인 odorant-gated ion channels comprised of a highly conserved co-receptor (Orco)는 중심 구멍 주위에 대칭 적으로 배열된 4 개의 서브 유닛을 갖는 homotetramer 채널 구조를 가지고 있다. 이는 인체 내에 존재하는 7-transmembrane receptor와 매우 유사한 구조를 형성하고 있고, 신경전달물질의 수용체와 매우 유사한 구조적 형태 및 gating mechanism을 가지고 있다. 본 연구에서는 초파리에서 분리한 후각 신경 수용체 하위 유형인 OR65 유전자 분리하여 세포 발현 시켜 Xenopus oocyte를 이용하여 Whole cell voltage clamp recording을 실시하였다. 본 수용체의 성공적인 발현 이후 유해 해충 유인제 개발 회사인 마이크로자임의 미생물 배양 추출물을 이용해서 후각 신경 수용체 활성 조절 여부를 연구하였다. 미생물 배양 추출물을 10,000배 희석한 recording media에서 수용체의 활성을 확인하였고, 이를 농도 별로 처리하여 농도 의존성 수용체 활성 작용을 확인하였다. 따라서 곤충의 후각 신경 수용체 활성 조절 시스템을 이용하여 유인물질 또는 기피 물질을 발굴할 수 있으며, 본 연구를 통해서 MZ01은 곤충 수용체 OR65를 활성 시킴으로써 유인 현상을 나타내며, 본 연구를 통해서 현장에서 검증된 미생물 배양 추출물의 성능을 과학적 분석으로 결과를 제시하였다.

방사선 방호의 목적 중 하나는 확률적 영향을 최소화 하는 것이다. PCXMC 2.0은 몬테카를로 시뮬레이션 기반의 프로그램으로 입사표면선량을 통해 유효선량과 암의 발병확률을 예측가능하게 해준다. 그렇기 때문에 선량계에 따른 입사표면선량 측정이 특히 중요하다. 본 연구는 반도체 선량계, 일반 선량계, 유리선량계를 통해 입사표면선량을 측정하고 그에 따른 결정 장기의 유효선량과 발병 확률을 비교분석 하는 것에 목적을 두었다. 실험방법은 두개부, 흉부, 복부의 선량계 별 입사표면선량을 측정하고 PCXMC 2.0을 통해 부위 별 결정 장기의 유효선량과 암의 발병 확률을 평가하였다. 그 결과 부위 별 입사표면선량은 동일한 조건임에도 일반 선량계, 반도체 선량계, 유리 선량계 순으로 차이가 났다. 이를 토대로 유효선량과 결정 장기의 암 발병 확률을 분석한 결과 또한 일반 선량계, 반도체 선량계, 유리 선량계 순으로 차이가 났다. 결론적으로 동일한 조건임에도 사용한 선량계에 따라 유효선량과 발병 위험도는 다르게 나타났음을 알 수 있었고, 본 연구를 통해 각각의 선량계에 따른 정확한 입사표면선량 모델을 제시하는 것이 중요하다는 것을 알 수 있었다.

Jeju Island is the highest rain-prone area in Korea that possesses affluent water resources, but future climate changes are predicted to further increase vulnerabilities as resultant of increasing of extreme events and creating spatial-temporal imbalance in water resources. Therefore, this study aimed to provide basic information to establish a proper water resources management plan by evaluating the effects of climate change on water resources using climate change scenario. Direct runoff ratio for 15 years (2000~2014) was analyzed to be 11∼32% (average of 23%), and average direct runoff ratio for the next 86 years (2015∼2100) was found as 28%, showing an increase of about 22% compared to the present average direct runoff ratio (23%). To assess the effects of climate change on long-term runoff, monthly runoff variation of future Gangjeong watershed was analyzed by dividing three time periods as follows: Present (2000∼2030), Future 1 (2031∼2070) and Future 2 (2071∼ 2100). The estimated results showed that average monthly runoff increases in the future and the highest runoff is shown by Future 2. Extreme values has been expected to occur more frequently in the future as compared to the present.

Estimation of runoff peak is needed to assess water availability, in order to support the multifaceted water uses and functions, hence to underscore the modalities for efficient water utilization. The magnitude of storm rainfall acts as a primary input for basin level runoff computation. The rainfall-runoff linkage plays a pivotal role in water resource system management and feasibility level planning for resource distribution. Considering this importance, a case study has been carried out in the Hancheon basin of Jeju Island where distinctive hydrological characteristics are investigated for continuous storm rainfall and high permeable geological features. The study aims to estimate unit hydrograph parameters, peak runoff and peak time of storm rainfalls based on Clark unit hydrograph method. For analyzing observed runoff, five storm rainfall events were selected randomly from recent years’ rainfall and HEC-hydrologic modeling system (HMS) model was used for rainfall-runoff data processing. The simulation results showed that the peak runoff varies from 164 to 548 m3/sec and peak time (onset) varies from 8 to 27 hours. A comprehensive relationship between Clark unit hydrograph parameters (time of concentration and storage coefficient) has also been derived in this study. The optimized values of the two parameters were verified by the analysis of variance (ANOVA) and runoff comparison performance were analyzed by root mean square error (RMSE) and Nash-Sutcliffe efficiency (NSE) estimation. After statistical analysis of the Clark parameters significance level was found in 5% and runoff performances were found as 3.97 RMSE and 0.99 NSE, respectively. The calibration and validation results indicated strong coherence of unit hydrograph model responses to the actual situation of historical storm runoff events.

A number of projects for development have been done continuously due to the increase of tourist in Jeju Island. However flood disaster countermeasure due to urbanization is not considered during this development projects. This study is to make basic process for the flood estimation in Han stream of Jeju Island. The variation of stream discharge due the every 5 years' land use change from 1980 to 2005. Data for flood events (rainfall and discharge) were collected for HEC-HMS model. Clark method was used for unit hydrograph analysis. For the estimation of Clark unit hydrograph parameters, Kraven II and Sabol’s empirical equations were applied. The peak discharge increased 9.9~33.67% and total discharge amount increased 12.53~30.21%. Also, time of concentration for peak discharge was reduced by 10 minutes for each event.

The water resources system of Jeju-do Island entirely depends on groundwater. This study is making a precision observation of baseflow, surface water, water shortage that might be vulnerable to climate change and drought in future. The field observation of baseflow discharges in Akgeuncheon stream has regularly been made with ADCP and ADC and Flowmate every two weeks for twenty-two (22) months (July 8, 2011 to April 27, 2013). This paper represent the results of calculating discharge of a number of hydraulic structures (broad-crested weirs) with comparing and has been calculated more accurate discharges with suitability of different observation methods. The average discharge has been observed 0.851 m3/s, whereas the average ADC and Flowmate is 0.709 m3/s. Meanwhile, stream discharge has been calculated 0.709 m3/s through the broad-crested weir equation. The discharge has calculated with the weir equation greatly changed according to even a small change in the water level. However, it showed a similar trend to one of the observed discharge. Although, in past there were generating errors caused by observers’ strides, vertical and horizontal flow velocity distribution when the average flow velocity had been measured, non-prismatic flow, turbulent flow and others in ADC. This study comes up with the weir equation is more suitable for the characteristics of Jeju-do could be presented through an observations of baseflow discharge.

This study is carried out to propose an empirical equation which can promptly predict the aircraft noise level at a specific point (a receptor) near Jeju international airport by using the information of the flight path data. For this purpose, Analyses of multiple linear regression with the slant distances (SD) calculated from the gate analyses of the flight path data, aircraft noise certification levels with unit of EPNL(effective perceived noise level) and noise levels measured at receptors are performed by SPSS package. From these regression analyses for approach and departure of aircraft, we can propose empirical equations which is statistically significant. The noise levels predicted by these empirical equations are highly correlated the measured data.

Jeju Island, the heaviest raining area in Korea, is a volcanic Island located at the southernmost of Korea, but most streams are of the dry due to its hydrological/geological characteristics different from those of inland areas. Therefore, there are limitations in applying the results from the mainland to the studies on stream run-off characteristics analysis and water resource analysis of Jeju Island. In this study, the SWAT(soil & water assessment tool) model is used for the Hwabuk stream watershed located east of the downtown to calculate the long-term stream run-off rate, and WMS(watershed modeling system) and HEC-HMS(hydrologic modeling system) models are used to figure out the stream run-off characteristics due to short-term heavy rainfall. As the result of SWAT modelling for the long-term rainfall-runoff model for Hwabuk stream watershed in 2008, 5.66% of the average precipitation of the entire basin was run off, with 3.47% in 2009, 8.12% in 2010, and root mean square error(RMSE) and determination coefficient(R2) was 496.9 and 0.87, respectively, with model efficient(ME) of 0.72. From the results of WMS and HEC-HMS models which are short-term rainfall-runoff models, unless there was a preceding rainfall, the runoff occurred only for rainfall of 40mm or greater, and the run-off duration averaged 10～14 hours.

Due to the difficulties for measuring flood discharge in the dangerous field conditions, conventional instruments with relatively low accuracy such as float still have been widely utilized for the field survey. It is also limited to use simple stage-discharge relationship for assessment of the flood discharge, since the stage-discharge relationship during the flood becomes complicated loop shape. In recent years, various non-intrusive velocity measurement techniques such as electromagnetic wave or surface images have been developed, which is quite adequate for the flood discharge measurements. However, these new non-intrusive techniques have little tested in the flood condition, though they promised efficiency and accuracy. Throughout the field observations, we evaluated the validity of these techniques by comparing discharge and velocity measurements acquired concurrently during the flood in a mountain stream. As a result, the flood discharge measurements between electromagnetic wave and surface image processing techniques showed high positive relationship, but velocities did not matched very well particularly for the high current speed more 3 m/s. Therefore, it should be noted here that special cares are required when the velocity measurements by those two different techniques are used, for instance, for the validation of the numerical models. In addition, authors assured that, for the more accurate flood discharge measurements, velocity observation as well as stage height is strongly necessary owing that the unsteady flow occurs during the flood.

The purpose of this study is to propose an appropriate method for marking out a boundary line of area damaged by aircraft noise. In an actual situation, there are many gaps between the boundary line of aircraft noise contour and the boundary line of an occupant of a house. Three cases faced in practice are considered in this study. Case Ⅰ is considered the land number together with sub-number. The Tong and Ban (the residential district number in Korea) are considered in Case Ⅱ. In Case Ⅲ, the configuration of ground is examined. The authors expect that the authority of aviation affairs should choose one of these cases and put in force in the near future.

The purpose of this study is to propose an appropriate criterium for proper sound insulation service preventing school classrooms from aircraft noise. The guideline of noise level for school facility by Korean school health law is less than Leq 55 which is less stringent than that of other countries. It has been reported that many students are annoyed and disturbed for their works when they are exposed to the noise level over Leq 55. It is desirable to modify this guideline of indoor noise level for school classroom. The noise level of classroom of which windows are opened was measured relatively high in case the school is located at the outside of the official area damaged by aircraft noise. In order to minimize the influence of aircraft noise on the school classrooms in the vicinity of domestic airport, it is necessary to improve the present criteria of WECPNL 75 for the establishment of the area damaged by aircraft noise from WECPNL 70.