목적: 안경광학과 전공 학생과 전문가를 대상으로 스마트 기반 국가시험에 대한 인식에 대하여 알아보고자 하였다. 방법: 안경광학 전공 학생, 교수, 임상전문가(안경사)로 구성된 171명을 대상으로 지필평가방식과 스마트 기반 멀티미디어 문항이 포함된 평가방식(SBT)에 대한 인식을 조사하기 위하여 설문조사를 실시하였다. 결과: 안경관련 전문가 집단의 SBT에 대한 인식은 다른 보건의료직종의 전문가 집단보다 낮은 것으로 나 타났지만, 안경사국가시험에서 지필평가와 SBT 평가 두 시험 체제는 차이가 없을 것이라는 의견이 44.4%, SBT 평가체제가 더 좋다는 의견이 37.4%로 지필평가(31.0%) 보다 높게 나타났다. 스마트 기반 시험의 가장 긍정적인 측면은 시험에 소요되는 시간 단축을 최대 장점으로 인식하는 것으로 나타났으며, 멀티미디어 문항의 긍정적인 측면은 임상현장에서의 수행능력을 효과적으로 평가할 수 있다는 것으로 나타났다. 또한, 교수집단의 경우 응시자의 임상수행능력을 보다 정확하게 평가할 수 있다, 졸업 후 임상현장에서 업무를 수행 하는 과정에 도움이 된다, 수행평가가 용이하다는 점에서 임상전문가보다 더 긍정적으로 반응하였다. 결론: 지필시험에서 사용하고 있는 선택형 시험문항은 문제해결능력보다는 직무를 수행하는데 필요한 관련 지식을 기억하거나 이해하고 있는지를 주로 평가하고 있어, 해당 직무의 실제성 또는 현실성을 반영하는 시험이 되기 위해서는 본 연구결과를 반영한 SBT 체제로의 전환이 필요한 것으로 나타났고, 이를 위한 타당성 연구가 지속적으로 이루어져야 할 것이다.

A full genomic DNA microarray technique was employed to investigate the effects of Dongchunghacho on aortal and hepatic gene expression in apolipoprotein E knockout mice fed a high-fat/high-cholesterol diet. Male 8- week - old ApoE-/- mice were randomly divided into two groups, control(high cholesterol group; HC) and supplementation of Dongchunghacho (SD). All of the mice were fed a high-fet/high cholesterol diet with or without Dongchunghacho supplemented by 1% for 6 weeks. At first, lipid profile of the Dongchunghacho was measured by biochemical analysis. No differences were observed in serum triglyceride and total cholesterol levels between the two groups. Antigenotoxic effect of the Dongchunghacho was measured by the single cell gel electrophoresis assay (Comet assay) and quantified as % fluorescence in tail. Dongchunghacho supplementation decreased significantly leukocytic DNA damage and also there was a tendency of reduction in hepatic DNA damage in Dongchunghacho group compared with the control group. In up regulated genes in liver and aorta of the mice, genes with 0 to 2- fold difference in expression level between the two group (HD and SD) was very much more in liver than in aorta, on the contrary, those with 2-fold to 16-flod difference increased greatly rather in aorta than in liver. Also, almost the same results were observed in down regulated genes in liver and aorta between the two groups. These results suggested that supplementation of Dongchunghacho might be helpful in preventing leukocytic DNA damage induced by high fat diet, and has a more crucial roles in aortal gene expression.

Although trisomy 16 is commonly detected in spontaneous abortions and accounts for over 30% of cases of autosomal trisomy detected after spontaneous abortion, trisomy 16 mosaicism is rarely detected by amniocentesis in the second trimester. Here, we report a case of level III trisomy 16 mosaicism (47,XX,+16[8]/46,XX[31]) diagnosed by cytogenetic analysis of independently cultured amniotic fluid cells. The female baby was delivered at full term with low birth weight and intrauterine growth retardation, and interestingly, her karyotype was normal (46,XX). Given the difficulty in predicting the outcomes of fetuses with this mosaicism, it is recommended to inform the possibility of mosaicisms including this trisomy 16 mosaicism during prenatal genetic diagnosis and genetic counseling for parents.

The aim of this study was to isolate chicken feather-degrading bacteria with high keratinolytic activity and to investigate cultural conditions affecting keratinolytic enzyme production by a selected isolate. A chicken feather-degrading bacterial strain CH3 was isolated from poultry wastes. Isolate CH3 degraded whole chicken feather completely within 3 days. On the basis of phenotypical and 16S rDNA studies, isolate CH3 was identified as Bacillus thuringiensis CH3. This strain is the first B. thuringiensis described as a feather degrader. The bacterium grew with an optimum at pH 8.0 and 37℃, where maximum keratinolytic activity was also observed. The composition of optimal medium for keratinolytic enzyme production was feather 0.1%, sucrose 0.7%, casein 0.3%, K2HPO4 0.03%, KH2PO4 0.04%, MgCl2 0.01% and NaCl 0.05%, respectively. The keratinolytic enzyme had a pH and temperature optima 9.0 and 45℃, respectively. The keratinolytic activity was inhibited ethylenediaminetetraacetic acid, phenylmethylsulfonyl fluoride, and metal ions like Hg2+, Cu2+ and Zn2+. The enzyme activated by Fe2+, dithiothreitol and 2-mercaptoethanol.