A strain of Amphidinium species was established from samples collected from the intertidal zone of a sandy beach of Jeju Island, Korea. Its cells were 13.0-15.0 μm in length and 10.0-13.0 μm in width. Its cell shape was round or oval and dorsoventrally flat. A pyrenoid was located in the center of the cell and a nucleus was posteriorly located. Its epicone was small and left-deflecting. Its cingulum had V-shape on the ventral side, forming a ventral ridge and extending to the sulcus. Polygonal amphiesmal vesicles and ring-shaped body scales not described previous were observed on the surface of the cell. Its morphological features were consistent with those of previously described Amphidinium fijiense. Phylogeny based on ITS region and LSU rDNA sequences revealed that this Amphidinium isolate was clearly clustered with other A. fijiense strains, but separated from other Amphidinium species. These results indicate that this Amphidinium isolate is A. fijiense. This study reports its presence for the first time in the intertidal zone of a sandy beach of Jeju Island, Korea.

Foot-and-mouth disease (FMD) is a very deadly and highly contagious disease that occurs in various cloven-hoofed animals. Korea imports vaccines including non-vaccinated serotype for the vaccine bank. The two company vaccines imported from 2019 to 2021 were evaluated through multiple tests based on national standard assay. SN titers for the vaccine of company A were more than 1.5 log10, those of company B vaccine in the geometric mean were at least 1.72. All imported vaccines exceeded the evaluation criteria, and the potency of each vaccine was above its own company standard. Stockpiled vaccines for emergency may help to control non-vaccinated serotypes of FMD outbreak nationally.

As the increase of chrysanthemum demand on chrysanthemum increases in Korea, the production of high quality chrysanthemum is needed. Chrysanthemum stunt viroid (CSVd) is one of the important viroid, which infects chrysanthemum and induces diseases that affects the decrease of quality and yield. To solve this problem, we used different size of meristem of chrysanthemum ‘Ency’ for shoot tip culture and also that of combined with heat treatment at 37οC. The efficiency of CSVd elimination was influenced by the size of shoot tip. The small-sized of meristems with 1 or 2 leaf primodia were regenerated into the highest number of CSVd-free plantlets. By RT-PCR, the 214-bp band corresponding to CSVd was not detected in 22.2% of the total number of tested regenerants from shoot tips with 2 leaf primordia. While, shoot tip culture combined with heat treatment of one-month-old in vitro shoots was not effective for CSVd-elimination. The CSVd-free plants grew more vigorously than CSVd-infected plants in the greenhouse.

Hexagonal barium ferrite () nano-particles have been successfully synthesised using selfassembly method. Diethyleneamine (DEA) surfactant was used to fabricate the micelle structure of Ba-DEA complex under various DEA concentrations. powders were synthesized with addition Fe ions to Ba-DEA complex and then heat treated at temperature range of 800-1000. The molar ratio of Ba/DEA and heat-treatment temperature significantly affected the magnetic properties and morphology of powders. powders synthesized with Ba/DEA molar ratio of 1 and heat-treated at 1000 for 1 hour showed the coercive forces (iHc) of 4.84 kOe with average crystal size of about 200 nm.

Nesfatin-1/NUCB2, which is secreted from the brain, is known to control appetite and energy metabolism. Recent studies have been shown that nesfatin-1/NUCB2 was expressed not only in the brain, but it was also expressed in the gastric organs and adipose tissue. However, little is known about the expression of nesfatin-1/NUCB2 in the male reproductive system. Therefore, we examined whether the nesfatin-1/NUCB2 and its binding site exists in the male reproductive organs. Nesfatin-1/NUCB2 mRNA and protein were detected in the mouse testis and epididymis by PCR and Western blot analysis. As a result of the immunohistochemistry staining, the nesfatin-1 protein was localized at the interstitial cells and Leydig cells in the testis. Nesfatin-1 binding sites were also displayed at boundary cells in the tunica albuginea. Furthermore, in order to examine if the expression of nesfatin-1/NUCB2 mRNA in the testis and epididymis were affected by gonadotropin, its mRNA expression was analyzed after PMSG administration into mice. NUCB2 mRNA expression levels were increased in both of the testis and epididymis after PMSG administration. These results demonstrated for the first time that nesfatin-1 and its binding site were expressed in the mouse testis and epididymis. In addition, nesfatin-1/NUCB2 mRNA expression was controlled by gonadotropin, suggesting a possible role of nesfatin-1 in the male reproductive organs as a local regulator. Due to this, further study is needed to elucidate the functions of nesfatin-1 on the male reproductive system.

물 부족을 해결하기 위한 방안으로 지표수-지하수 연계이용에 관한 연구가 최근 국내에서도 관심을 끌고 있다. 지역 여건을 최대한 활용하면서 동시에 친환경적인 지표수와 지하수 연계이용은 통합적인 수자원 관리에 기여하는 바가 클 것으로 예측된다. 본 연구에서는 선행 논문에서 개발된 방법론 즉, 지표수-지하수 연계이용이 시행되어야 할 지역의 선정, 가뭄분석을 통한 물 공급 시나리오 도출, 연계이용 결과 얻어지는 추가 수자원확보량 산정까지 전과정의 현장적용성을

가뭄이나 장래 물 부족을 해결하기 위한 방안으로 지표수-지하수 연계이용 방안이 대두되고 있다. 하나의 수원에 지나치게 높게 의존하고 있는 지역은 물 부족사태가 발생할 개연성이 높기 때문에 연계이용을 검토할 수 있으며, 추가 및 신규 수자원 확보 차원에서도 연계이용은 매우 현실적이고 효율적인 대안이 될 수 있다. 본 연구에서는 가뭄과 같은 비상시 안정적인 물 공급을 위해 지표수와 지하수를 효율적으로 연계하여 추가적인 수자원을 확보하는데 적용할 수 있는 방