The development of drugs from natural plant sources is at growing interest due to the limitations of chemical drugs in terms of side effects and cost-effective factors of natural medicines. Among the various components contained in natural plant materials, flavonoids are of increasing interest because of their extended biological benefits. Flavonoids are classified into various types according to their structure and possess different activities depending on the structure. In this study, the flavonoids contained in Artemisia, native to Korea were examined and reviewed. HPLC chromatograms of three Artemisia species (Artemisia annua L., Artemisia iwayomogi and Artemisia argyi H.) were examined from published sources and their component analysis by MS data were summarized. The various flavonoids of Artemisia were classified into 12 types according to the main structure, and 10 flavonoids based on various activities were examined. The 10 flavonoids were identified as quercetin, kaempferol, rhamnetin, diosmetin, luteolin, methoxyflavone, catechin, apigenin, malvidin and genkwanin with extensive reported studies till date. The ten flavonoids examined have been reported to be effective in preventing and treating various diseases and exhibit activities such as anti-cancer, anti-inflammatory, antioxidant, antiviral, anti-obesity, anti-diabetic and anti-Alzheimer. The collective results from the reported studies suggest that the three types of Korean native Artemisia, contains various flavonoids with beneficial activities and may have therapeutic effects against diseases.

Inflammation is an important protective response mechanism that occurs against microbial invasion or injury. However, excessive inflammation may lead to cause of morbidity and mortality in diseases. The activated macrophages plays a vital role in inflammatory response by stimulation of lipopolysaccharide (LPS) and tumor necrosis factor-α (TNF-α). This activation further damages the host by inducing certain pro-inflammatory mediators such as nitric oxide (NO), interleukin-1β (IL-1β), interleukin- 6 (IL-6), TNF-α, inducible nitrous oxide (iNOS) and cyclooxygenase (COX-2). Flavonoids are bioactive compounds with potential effects as anti-cancer, anti-inflammation, anti-viral and anti-bacterial activities. Polymethoxyflavones (PMFs) are unique to citrus plants which are of specific interest owing to their biological effects that includes lipoprotein metabolism and anti-inflammatory activity. Sinensetin is one of the PMFs having five methoxy groups on the basic benzo-γ-pyrone skeleton with a carbonyl group at the C4 position. Sinensetin have been known for exerting various pharmacological activities including anti-angiogenesis, anti-diabetic and anti-inflammatory activities. However, there are no studies focused on the anti-inflammatory effects of sinensetin on skeletal muscle cells. In the present study, we investigated the antiinflammatory effect of flavonoids isolated from Sinensetin on the production of pro-inflammatory mediators mediated by nuclear factor-kappa B (NF-κB) by inhibition of signal transduction in LPS - induced L6 skeletal muscle cells.

본 연구는 사양체계 및 TMR의 NDF 수준이 번식용 젖소 육성우의 혈중 대사물질 농도 변화에 미치는 영향을 검토하고, 에너지, 단백질, 지질 및 간 기능 관련 지표 대사물질의 농도를 토대로 영양 상태를 관리하기 위한 기초자료를 제공하기 위해 수행되었다. 공시동물은 번식용 젖소 육성우 50두를 공시하였으며, 시험구 배치는 사양체계에 따라 TMR 처리구(TMR) 및 배합사료 + 혼합 조사료 처리구(CON)의 2처리로 하였으며, TMR의 NDF 수준에 따라 53% 이상(T1), 50 ∼ 53%(T2) 및 50% 이하(T3)로 3처리 하였다. 혈중 GLU 농도는 사양체계에 영향을 받지 않았지만, NDF 수준이 감소될수록 유의적으로 증가하였다(p<0.05). 사양체계가 혈중 단백질 관련 대사물질 농도에 미치는 영향은 없었으며, NDF 수준이 낮아질수록 혈중 ALB 농도가 증가되는 결과를 보였다(p<0.05). 혈중 CHOL 농도는 TMR 보다 CON 처리구에서 높았으며 (p<0.05), NDF 수준이 낮을수록 증가되는 결과가 나타났다. 혈중 지질 농도는 TMR 보다 CON에서 높았으며, NDF 수준이 낮아질수록 높게 나타났다. 혈중 ALT 농도는 T3구가 T1 및 T2구에 비해 유의적으로 낮게 나타났다(p<0.05). 따라서 본 연구의 결과에서 사양체계 및 TMR의 NDF 수준은 번식용 젖소 육성우의 혈중 대사물질 농도 변화에 영향을 미칠 수 있지만, 사료섭취량 및 영양성분 수준을 고려할 필요가 있을 것으로 판단된다.

In the United States and Europe, new environmentally friendly asphalt pavement has been researched as an alternative to traditional hot asphalt pavement. After the Paris Convention of 2015, policies should be found to reduce carbon dioxide. In the field of asphalt pavement, new methods are needed to reduce carbon dioxide from the traditional hot asphalt pavement. In Korea, waste asphalt is growing and natural aggregate is running dry. So the government is implementing policies to increase the use of waste resources. So, it created a new asphalt pavement method to reduce CO2 and use waste asphalt. It is a cold recycled asphalt pavement. Emulsified asphalt has a balance of dispersibility, stability, and adhesive between water, aggregates, and asphalt. But, the physical properties of emulsified asphalt can be degraded compared to traditional hot asphalt pavement. So there are limitations in actual use. The study compared the softening point, elastic recovery, and penetration properties of asphalt mix compounds by using latex in emulsified asphalt. In particular, cations latex was used for the emulsified asphalt, which could further improve the physical properties.

The composition of the deicer sprayed on the highway is spreading over the highway by the scattering or snow removal activity, or car movement and consequently affecting the vegetation environment around the highway. These are the cause of the damage of fruit trees and crops, and also the cause of corrosion of highway structures. The goal of this study is to estimate the detected range of deicer components from a highway. The concentration of the deicer components in gauze and soil were investigated according to the crossing distance from the highway. The data collected were then used to estimate the concentration range of deicer components in a rage of distance up to 100 m from the highway where the deicer was spread. The sample time and weight of gauze were measured before and after installation, and the soil was collected at more than three points in parallel with the highway at the gauze installation point. The components in gauze and soil were investigated in addition to the deicer components (Ca2+, Na+, Cl-) as well as Mg2+ and K+. As Ca2+ and Cl- components of deicer were affected by agricultural use, Na+ component was selected as a tracer and further SAR (Sodium Adsorption Ratio) of soil was analyzed to examine the degree of influence on vegetation indirectly. The gauze concentration was evaluated by the concentration of the deicer ingredient at the background concentration of the blank gauze. The total amount of the deicer sprayed in the study road for 4 months (winter season) was about 93 ton/km. In the gauze test, the spread of the deicer was detected at a distance of 100 m in study area, but the concentration of the deicer in the gauze by distance decreased rapidly within 10 m from the highway. And concentration of the deicer components in gauze and soil came down after rainy season (August ~ Sep.). The results showed that the components of the deicer could be spread widely by the wind. The effective range of the deicer on vegetation based on SAR in soil was estimated to be less than 20 m from the highway. This study examines the concentration changes of the deicer components in gauze and soils and shows that deicer components sprayed on the highway are accumulated and moved over time by wind, snow removal, terrain, water system and land use around the highway.

본 연구에서는 김치 제조시 철저한 재료 세척의 필요성을 확인하고, 더불어 비가열 식품인 김치의 위생 안전성 실태를 파악하여 효율적 미생물 저감화 방안을 마련하고자 국내에서 유통되는 김치 원·부재료 200건의 세척 전과 후의 비교분석과 시판 김치 100건에 대하여 미생물 분석을 실시하였다. 김치 원·부재료 및 완제품에 대하여 일반 세균수, 대장균 및 대장균군을 모니터링 하였으며, 병원성 미생물 9종(Bacillus cereus, Clostridium perfringens 정성·정 량, Staphylococcus aureus, Enterohemorrhagic Escherichia coli, Campylobacter jejuni/coli, Yersinia enterocolitica, Salmonella spp., Vibrio parahaemolyticus, Listeria monocytogenes 정성)을 분석 하였다. 모니터링 결과, 김치 원· 부재료 및 완제품에서 일반세균은 1.4~9.0 log CFU/g 수준 으로 확인되었으며, 원·부재료 중 생강에서 8.8 log CFU/g, 완제품 중 총각김치에서 9.0 log CFU/g으로 일반세균이 가장 높게 나타났다. 대장균군은 0.5~7.3 log CFU/g으로 확 인되었으며, 생강에서 7.3 log CFU/g으로 가장 높게 나타 났고, 병원성 미생물 9종의 분석결과, Bacillus cereus를 제 외한 나머지 8종의 식중독균은 모든 시료에서 검출되지 않았다. 김치 제조 시 사용되는 김치 원·부재료들의 세척 공정 후 미생물 수는 일반세균수 0.2~3.2 log CFU/g, 대장 균군 0.3~2.7 log CFU/g, Bacillus cereus 1.0~3.9 log CFU/g 감소하였다. 따라서, 김치 원·부재료의 세척 공정으로 미생물 오염도를 감소 시켰으며, 이 결과를 통하여 김치 완 제품의 위생 및 저장성 증진에 기여 할 수 있을 것으로 확인되었다.

The effects of cold storage temperature and exposure duration on immature stages of spotted-wing drosophila (SWD) Drosophila suzukii (Diptera: Drosophilidae) on ‘Campbell Early’ grapes were examined to establish a phytosanitary control method. The immature stages (eggs, larvae and pupae) of SWD were all dead after a 6-day cold treatment at 1°C and 8-day cold treatment at 1.5 and 2°C. Small-scale tests using pupae, which were the most-cold tolerant stage, confirmed the validity of the selected temperature and exposure durations. Conversely, the 8- and 10-day at 1°C treatments showed 100% mortality, suggesting that these treatments can guarantee quarantine security against infestations of SWD on exported ‘Campbell Early’ grapes.

The aim of this study was to select various fungal strains indigenous to Korea that have the potential to produce cellulases, including filter paper activity (FPase), endo-β-1,4-glucanase (EG), and β-glucosidase (BGL). Among the 25 species of Ascomycetes and the 32 species of Basidiomycetes tested in this study, the Bjerkandera adusta KUC10565, Heterobasidion orientale KUC10556, Hyphoderma praetermissum KUC10609, and Trichoderma harzianum KUC1716 all exhibited remarkably high FPase activity. In addition, the T. harzianum KUC1716 showed high levels of EG and BGL activity. This strain has been selected for further study because of their enzymatic potential.

Elderly with dementia in geriatric hospitals (EDGH) are highly dependent on hospital meals. This study evaluated the foodservices satisfaction and food preference of the EDGH. The survey was conducted on 104 elderly with dementia (21 males and 83 females) in 4 geriatric hospitals in Seoul and Incheon in November, 2016. Data were collected from interviews using a questionnaire that consisted of 6 questions for foodservice satisfaction and 24 questions (10 categories) for food preference. The data were analyzed using SPSS ver. 20.0. The satisfaction with taste, saltiness, texture, and variety in foodservices was good, but the satisfaction with the amount was not, and the reason for leaving food was its large serving size. The subjects preferred soft boiled rice, noodles, porridges, meats, fish, seafood, vegetables, and fruits. Among them, they preferred more janchiguksu, red bean porridge, beef, croaker, oyster, spinach, and banana. Regarding the cooking methods, they preferred soup, grill, and boiling, but not frying. The also preferred Chinese cabbage kimchi, but they did not prefer hard kkakdugi. They did not prefer milk because of diarrhea, but they preferred yogurt. Therefore, to provide a satisfying meal for EDGH, it is necessary to develop a friendly diet considering their food preferences.

Ficus carica L. (common fig), one of the first plants cultivated by humans, originated in the Mediterranean basin and currently grows worldwide, including southwest Asia and South Korea. It has been used as a traditional medicine for treatment of metabolic, cardiovascular, and respiratory diseases as well as hemorrhoids and skin infections. Its pharmacological properties have recently been studied in detail, but research on the anti-cancer effect of its latex has been only been studied on a limited basis on several cell lines, such prostate cancer, breast cancer, and leukemia. In this study, we investigated the anti-cancer activity of the latex of Ficus carica L.and its underlying mechanism in FaDu human hypopharynx squamous carcinoma cells. (See Ed. note above) We confirmed through SDS-PAGE analysis and gelatinolytic activity analysis that the latex of Ficus carica contains cysteine protease ficin. Our data showed that the latex inhibited cell growth in a dose-dependent manner. In addition, the latex treatment markedly induced apoptosis in FaDu cells as determined by FACS analysis, elevated expression level of cleaved caspase-9, -3 and PARP (poly (ADP-ribose) polymerase), and. increased the expression of Bax (pro-apoptotic factor) while decreasing the expression of Bcl-2 (anti-apoptotic factor). Taken together, these results suggested that latex containing the ficin inhibited cell growth and induced apoptosis by caspase and the Bcl-2 family signaling pathway in FaDu human hypopharynx squamous carcinoma cells. These findings point to the potential of latex of Ficus carica to provide a novel chemotherapeutic drug due to its growth inhibition effects and induction of apoptosis in human oral cancer cells.

Colorectal cancer is a major cause of morbidity and mortality that accounts for over 9% of all incidences of cancer. Additionally, colorectal cancer is widely recognized as an environmental disease related to ill-defined cultural, social and lifestyle factors including physical activity, obesity, cigarette smoking and heavy alcohol consumption. Accordingly, natural phytochemicals and extracts have attracted attention because of their beneficial biological effects. Coenzyme Q10 (CoQ10) is a common supplementary medicine applied to increase bioenergetic capacity in various diseases. Therefore, in this study, we investigated whether CoQ10 treatment has any inhibitory effects and its related cellular mechanisms in human colon cancer HCT116 cells. A MTT assay revealed that CoQ10 slightly decreased the proliferation of HCT116 cells; however, glutathione- and superoxide dismutase- activity were unchanged in response to CoQ10 treatment. A DCF-DA assay revealed that CoQ10 slightly increased ROS release of HCT116 cells. However, in a nitric oxide (NO) assay, CoQ10 significantly increased NO production in a dose-dependent manner. The results of western blot analysis revealed that the protein levels of Bax, p21 and p53 were increased, whereas the protein level of Bcl2 was decreased suggesting that the CoQ10-mediated inhibitory mechanism is associated with apoptotic signaling. Taken together, our findings indicate that CoQ10 has an inhibitory effect on the growth of colon cancer cells via NO production that is associated with regulation of factors involved in apoptotic signaling including Bax, Bcl2, p21 and p53.

본 연구는 반추동물용 섬유질배합사료(TMR) 제조업체 의 사료안전관리 인식 및 사료 중 위해인자 오염도를 조사 하기 위해 138개소 TMR 제조시설을 대상으로 약 10개월 동안 설문조사를 실시하였다. 조사표는 총 17개 문항으로 구성하였으며, 얻어진 자료는 기술통계분석, 교차분석 및 신뢰도 검정을 통해 분석하였다. TMR 제조시설의 평균 일 일 생산능력은 81.38톤 이었다. 평균 개업연도는 2004년 및 종사인원은 13.7명이었고, 유통경로는 직접거래가 80.4%로 가장 많았다. 사료안전관리 시스템 도입은 사료안전관리의 제도적 여건이 조성된 후 도입이 필요하다는 인식이 주를 이루었으며, 사료안전관리 시스템 구축을 위해서는 사료안 전정보 제공 및 공유가 가장 필요한 조건으로 나타났다. 사료안전성 및 위해물질 관리에 가장 취약한 분야는 곰팡 이 독소인 것으로 조사되었으며, 사료 제조과정에서 가장 위험한 위해인자는 쇠, 파편, 흙 등과 같은 이물질로 나타 났다. 사료 중 위험인자 오염도 조사에서 카드뮴, 비소 및 셀레늄은 허용기준치를 초과한 제품이 일부 조사되었고, 오크라톡신류 및 아플라톡신류는 허용범위내로 조사되었 다. 잔류농약, BSE, 푸모니신 및 살모넬라는 검출되지 않았 으며, 멜라민 및 비단백태질소화합물은 각각 11.35ppm 및 5%로 최댓값을 나타냈다. 규모별 TMR 제조시설의 사료 내 위해인자 오염도는 규모별로 차이가 많았으며, 같은 규 모 내에서도 제조시설에 따라 위해인자의 검출량이 다르 게 나타났다. 또한 소규모에 비해 중규모 및 대규모에서 위해인자 검출량이 더 높아지는 경향을 보였다. 따라서 본 조사결과 TMR 제조시설의 사료안전관리 시스템을 구축하 기 위해서는 사료안전에 대한 정보제공 및 공유가 정책적 으로 제도화되어야 하고, 사료 내 발생하는 곰팡이 독소방 지 및 이물질 제거를 위한 사료관리법의 강화와 원료 공급 처에서 이물질을 제거할 수 있는 장치 도입을 의무화할 필 요가 있다고 판단된다.

Anthricin (Deoxypodophyllotoxin), a naturally occurring flavolignan, has well known anti-cancer properties in several cancer cells, such as prostate cancer, cervical carcinoma and pancreatic cancer. However, the effects of Anthricin are currently unknown in oral cancer. We examined the anticancer effect and mechanism of action of Anthricin in human FaDu hypopharyngeal squamous carcinoma cells. Our data showed that Anthricin inhibits cell viability in a dose- and time-dependent manner (IC50 50 nM) in the MTT assay and Live & Dead assay. In addition, Anthricin treated FaDu cells showed marked apoptosis by DAPI stain and FACS. Furthermore, Anthricin activates anti-apoptotic factors such as caspase-3, -9 and poly (ADP-ribose) polymerase (PARP), suggesting that caspase-mediated pathways are involved in Anthricin- induced apoptosis. Anthricin treatment also leads to accumulation of the pro-apoptotic factor Bax, followed by inhibition of cell growth. Taken together, these results indicate that Anthricn-induced cell death of human FaDu hypopharyngeal squamous carcinoma cells is mediated by mitochondrial-dependent apoptotic pathway. In summary, our findings provide a framework for further exploration on Anthricin as a novel chemotherapeutic drug for human oral cancer.