Ainsliaea acerifolia leaves are registered with the Ministry of Food and Drug Safety as edible herbal materials in Korea, and research is underway to explore their potential in developing functional foods, cosmetics, and pharmaceuticals. In this study, we developed an analytical method using HPLC-DAD to quantify three key compounds—chlorogenic acid, isochlorogenic acid A, and 1,5-dicaffeoylquinic acid—in A. acerifolia leaves extract. This method has been optimized and validated for specificity, accuracy, precision, limit of quantification (LOQ), and linearity. The correlation coefficients (r²) for the calibration curves exceeded 0.9962. The limits of detection (LOD) and quantification (LOQ) were 0.3012 and 0.9128 μg/mL for chlorogenic acid, 0.1182 and 0.3582 μg/mL for isochlorogenic acid A, and 0.2342 and 0.7098 μg/mL for 1,5-dicaffeoylquinic acid, respectively. The net recovery rates for accuracy testing were 105.13% for chlorogenic acid, 105.37% for isochlorogenic acid A, and 100.37% for 1,5-dicaffeoylquinic acid. All parameters assessed with this newly developed method fell within the acceptable ranges specified by ICH guidelines. These findings demonstrate that the method is robust and reliable for accurately identifying and quantifying chlorogenic acid, isochlorogenic acid A, and 1,5-dicaffeoylquinic acid in both routine analysis and large-scale extraction process of A. acerifolia leaves.
한강 3개 보 구간 수변부 11개 지점을 대상으로 2023년 총 2회 채집된 저서성 대형무척추동물은 총 100종 평균 426.0 개체/m2였다. 여주보에서 64종으로 가장 많이 출현하였고, 강천보에서 59종, 이포보에서 54종, 한강보상류 에서 41종, 한강보하류에서 32종이 확인되었다. 전체 평균 개체밀도는 강천보에서 944.1 개체/m2로 가장 많았고, 한강보상류에서 282.7 개체/m2, 여주보에서 251.9 개체/m2, 이포보에서 180.6 개체/m2, 한강보하류에서 172.9 개체 /m2 순으로 확인되었다. 한강 보 구간에서는 플라나리아류, 실지렁이, 깔따구류 등이 우점함. 보구간의 특성상 상대적으로 단순한 하상 및 깊은 수심에 적응성이 높은 실지렁이, 깔따구류의 우점은 일반적인 특성으로 볼 수 있으며 일시적으로 수변부에서 밀집된 플라나리아류의 채집이 이뤄져 우점된 결과를 보인 것으로 보임. 한강 보 구간 수변부 및 중앙부 조사지점별 군집분석을 실시한 결과 한강보 하류는 3개 보에 비해 우점도가 높고 다양도 와 풍부도가 낮았으며 전반적으로 상류부에 위치한 한강보상류 등의 지점에서 군집안정성이 확보되고 있는 것으로 보이며 이러한 점은 상대적으로 다양한 미소서식환경에 기인한 것으로 판단된다.
Although ethylformate and phosphine fumigants are widely used for pest quarantine, studies related to their mechanism of action and metabolic physiological changes in Drosophila models are still unclear. In this study, we investigated how key metabolites altered by fumigants and cold treatment are associated with and affect insect physiology by comparative metabolome analysis. Fumigant treatment significantly altered cytochrome P450 and glutathione metabolites involved in the detoxification mechanism and showed lower expression of PGF2α involved in the immune response compared to the control. Additionally, most of the metabolites functioned in metabolic pathways related to the biosynthesis of amino acids, nucleotides and cofactors.
The growth of OTT internet and mobile video streaming services is a major development in consumption of global media sport. The online streaming of sporting events has become the important way of copyrights holders to engage sport fans, especially young fans. The most popular live streaming platforms in South Korea, such as NAVER and Africa TV are intervening in coverage rights markets and changing how live sport is experienced and shared across television, computer, tablet and smartphone screens. At the FIFA World Cup Qatar 2022, NAVER's live streaming service reached 121,173,945 cumulative viewers in 64 matches, including the opening ceremony and the final between France and Argentina. Based on suspense theory, this study aimed to examine the factors that influence sport fans’ watching intention to stream online sport. The current study collected the number of cumulative viewers of live streaming from NAVER and Africa TV, for the matches broadcasted across the KBS, MBC, and SBS, a total of 64 matches in the FIFA World Cup Qatar 2022. The results suggest that a measure of the unfolding nature of suspense is a stronger predictor of watching intention than those used previously. The additional measures of suspense that take into account the unfolding events of the contest will be stronger predictors of watching intention than either disposition toward the winning team or closeness of final score.
In this study, we have fabricated the phenolic resin (PR)/polyacrylonitrile (PAN) blend-derived core-sheath nanostructured carbon nanofibers (CNFs) via one-pot solution electrospinning. The obtained core-sheath nanostructured carbon nanofibers were further treated by mixed salt activation process to develop the activated porous CNFs (CNF-A). Compared to pure PAN-based CNFs, the activated PR/PAN blend with PR 20% (CNF28-A)-derived core-sheath nanostructured CNFs showed enhanced specific capacitance of ~ 223 F g− 1 under a three-electrode configuration. Besides, the assembled symmetric CNF28-A//CNF28-A device possessed a specific capacitance of 76.7 F g− 1 at a current density of 1 A g− 1 and exhibited good stability of 111% after 5,000 galvanostatic charge/discharge (GCD) cycles, which verifies the outstanding long-term cycle stability of the device. Moreover, the fabricated supercapacitor device delivered an energy density of 8.63 Wh kg− 1 at a power density of 450 W kg− 1.