국내에서 유통되는 한약재 오공(蜈蚣)의 정·위품 유통 현황 파악과 유전자 감별법 개발을 위해 서로 다른 6개 유통사에서 오공으로 판매중인 전형약재를 구매하여 각 약재 포장 단위별 크기, 색깔, 무늬 등 형태적으로 차이가 있는 개체를 분류하여 국내에서 채집된 왕지네 표본 2개체를 포함 총 30개 시료를 대상으로 DNA 바코드 분석을 실시하였다. 확보한 미토콘드리아 COI 염기서열 정보와 기 등재된 NCBI GenBank 염기서열 정보를 이용하여 계통 분석을 실시한 결과, 28개 약재 시료 중 국산 및 중국산 전형약재 유통품 13 개체는 모두 대한민국약전외한약 (생약)규격집에 정품 기원종으로 수재된 Scolopendra subspinipes mutilans로 확인되었으며, 이들은 국내 채집 왕지네 개체들과 함께 하나의 단계통군을 형성하였다. 하지만 인도네시아산 전형약재 유통품 15 개체의 경우 4개의 그룹으로 구분되었는데, 그 중 3개 그룹은 S. dehaani, S. subspinipes, 그리고 명확한 종을 알 수 없는 Scolopendra sp.로 Scolopendra 속으로 확인되었고 나머지 그룹을 형성하는 한 개체는 Scolopendra 속에 속하지 않고 Rhysida singaporiensis와 89%의 유사도를 보였다. COI 바코드 분석을 통해 국내 유통되는 오공은 원산지가 한국 또는 중국인 경우 모두 정품 기원종으로 확인되었으며, 원산지가 인도네시아인 경우에는 모두 위품인 것으 로 확인되었다. 또한 위품으로 확인된 유통약재는 총 4개의 종으로 분류되었고, 대부분은 정품인 Scolopendra속 의 분류군이었으며 Rhysida속과 가까운 분류군도 오공으로 수입되어 유통되고 있는 것으로 확인되었다.
Scaly-leg in chickens, also known as knemidokoptiasis, is caused by infection with Knemidokoptes spp. Chickens in backyard systems developed scaly skin lesions on their legs. This farm is not an industrialized farm for producing meat or eggs. A skin smear microscopic test identified the Knemidokoptes spp. mites, revealing both larvae and adults in the same chicken. Ivermectin was applied to control the knemidokoptiasis through oral and topical administration. However, there were no clinical effects observed after the application of ivermectin. Knemidokoptiasis worsens the quality of life in chickens. Effective control methods could be established in further studies.
In the summer of 2018, the Korea-Japan (KJ) region experienced an extremely severe and prolonged heatwave. This study examines the GloSea6 model's prediction performance for the 2018 KJ heatwave event and investigates how its prediction skill is related to large-scale circulation patterns identified by the k-means clustering method. Cluster 1 pattern is characterized by a KJ high-pressure anomaly, Cluster 2 pattern is distinguished by an Eastern European highpressure anomaly, and Cluster 3 pattern is associated with a Pacific-Japan pattern-like anomaly. By analyzing the spatial correlation coefficients between these three identified circulation patterns and GloSea6 predictions, we assessed the contribution of each circulation pattern to the heatwave lifecycle. Our results show that the Eastern European highpressure pattern, in particular, plays a significant role in predicting the evolution of the development and peak phases of the 2018 KJ heatwave approximately two weeks in advance. Furthermore, this study suggests that an accurate representation of large-scale atmospheric circulations in upstream regions is a key factor in seasonal forecast models for improving the predictability of extreme weather events, such as the 2018 KJ heatwave.
Drosophila suzukii (Diptera, Drosophilidae) can damage thin-skinned fruits (plums, cherries, peaches, blueberries, and strawberries) by laying eggs inside the fruit. In this study, a basic experiment was conducted to investigate the preference of D. suzukii to export table grape varieties. Four varieties of grapes (Kyoho grape, Shine muscat, Campbell grape, and Black sapphire) were placed in a cage containing more than 2,500 number of D. suzukii adults for 6, 8, 10, 12, and 24 hours and the egg-laying in grape has been induced. After 2 weeks, the number of pupae that emerged was counted. As a result, the largest number of pupae was generated in Kyoho grape among the four types of grapes, indicating that D. suzukii prefers to lay egg in Kyoho grape. Through this experiment, it was determined that 6 h was the appropriate inoculation time, and then an inoculation experiment was conducted on Kyoho grape. The total weight of the Kyoho grape used in the experiment was 36.119 kg, and a total of 2594 pupae were generated. The average number of D. suzukii per cluster was about 50. All stages of D. suzukii treated with low temperature(1℃) for 10 days were completely controlled.
The prolonged and heavy East Asian summer precipitation in 2020 may have been caused by an enhanced Madden-Julian Oscillation (MJO), which requires evaluation using forecast models. We examined the performance of GloSea6, an operational forecast model, in predicting the East Asian summer precipitation during July 2020, and investigated the role of MJO in the extreme rainfall event. Two experiments, CON and EXP, were conducted using different convection schemes, 6A and 5A, respectively to simulate various aspects of MJO. The EXP runs yielded stronger forecasts of East Asian precipitation for July 2020 than the CON runs, probably due to the prominent MJO realization in the former experiment. The stronger MJO created stronger moist southerly winds associated with the western North Pacific subtropical high, which led to increased precipitation. The strengthening of the MJO was found to improve the prediction accuracy of East Asian summer precipitation. However, it is important to note that this study does not discuss the impact of changes in the convection scheme on the modulation of MJO. Further research is needed to understand other factors that could strengthen the MJO and improve the forecast.
본 연구는 불법적으로 식품에 사용될 수 있는 부정물질 11종에 대한 안전관리 강화를 위해 정량 및 정성 분석이 가능한 HPLC-DAD와 LC-MS/MS를 검증하기 위해 수행 되었다. 확립된 시험법은 AOAC 가이드라인에 따라 직선성, 정밀성, 정량한계 및 회수율 등을 통해 유효성을 확인 하였다. 본 실험에서 정량한계를 포함하여 검량선을 작성 하였고, 모두 0.99 이상의 직선성을 확인하였다. 또한 정확성은 LC (90.0-106%), LC-MS/MS (83.0-114%) 이고, 정 밀도는10% 이하로 재현성이 우수하였다. 확립된 시험법은 식품 중 부정물질 안전관리 및 모니터링에 활용될 것으로 사료된다.