식품 포장 분야에서 바이오센서와 바이오폴리머 기반 나 노복합체, 즉 바이오나노복합체의 통합이 점차 산업 전문 가들에 의해 인식되고 있으며, 이는 식품의 품질과 안전 에 대한 우려가 증가함에 따라 주도되고 있습니다. 식품 포장에 내장된 바이오센서는 포장된 상품의 미생물에 의 한 변질을 지속적으로 모니터링함으로써 식품의 완전성을 유지하는 핵심 요소로 업계를 변화시킬 준비가 되어 있다. 동시에, 탁월한 기계적, 열적, 광학적, 항균적 특성으로 인 해 바이오폴리머 기반 나노복합체의 연구와 적용이 크게 확대되었다. 이러한 특성은 이들을 혁신적인 포장 솔루션 에 적합한 주요 재료로 만든다. 그러나 지능형 식품 포장 시스템 발전에 바이오센서와 바이오나노복합체를 사용하 는 잠재적인 장애물과 전망을 탐구하는 것은 아직 충분하 지 않다. 바이오나노복합체와 바이오센서의 융합을 제안 하는 것은 스마트 포장 산업을 재정의하는 획기적인 단계 로, 이 기술들을 더 깊이 이해하여 지속 가능하고 경제적 으로 실행 가능한 스마트 포장 옵션의 개발을 촉진할 필 요성을 강조한다. 이 리뷰는 바이오센서와 바이오나노복 합체에 대한 기존 연구와 개발 동향을 철저히 검토하고, 가까운 미래에 스마트 식품 포장 산업에서 진전을 이끌어 낼 앞으로의 도전과 기회를 강조하는 데 전념하고 있다.

Black ice, a thin and nearly invisible ice layer on roads and pavements, poses a significant danger to drivers and pedestrians during winter due to its transparency. We propose an efficient black ice detection system and technique utilizing Global Positioning System (GPS)-reflected signals. This system consists of a GPS antenna and receiver configured to measure the power of GPS L1 band signal strength. The GPS receiver system was designed to measure the signal power of the Right-Handed Circular Polarization (RHCP) and Left-Handed Circular Polarization (LHCP) from direct and reflected signals using two GPS antennas. Field experiments for GPS LHCP and RHCP reflection measurements were conducted at two distinct sites. We present a Normalized Polarized Reflection Index (NPRI) as a methodological approach for determining the presence of black ice on road surfaces. The field experiments at both sites successfully detected black ice on asphalt roads, indicated by NPRI values greater than 0.1 for elevation angles between 45o and 55o. Our findings demonstrate the potential of the proposed GPS-based system as a cost-effective and scalable solution for large-scale black ice detection, significantly enhancing road safety in cold climates. The scientific significance of this study lies in its novel application of GPS reflection signals for environmental monitoring, offering a new approach that can be integrated into existing GPS infrastructure to detect widespread black ice in real-time.

This study proposes a soil moisture retrieval method from ground reflection signals received by Global Positioning System (GPS) antenna modules consisting of an up-looking (UP) right-hand circular polarization (RHCP) and two down-looking (DW) RHCP and left-hand circular polarization (LHCP) signals. Field experiments at four different surface types (asphalt, grassland, dry soil, and moist soil) revealed that the DW RHCP and LHCP signals are affected by antenna height and multipath interference signals. The strength differences between the DW LHCP and UP RHCP signals were in good agreement with the DW LHCP signals. Methodologically, this study applied a spectrum analysis to the detrended surface-reflected signals for RHCP and LHCP. The study indicated that the down-looking antenna exhibited greater sensitivity to reflected GPS signals than the up-looking antenna. We demonstrated the feasibility of estimating soil moisture using GPS signals, by comparing LHCP signals received by the down-looking antenna with theoretical values. This study presents a novel method for estimating soil moisture in vegetated areas, leveraging the advantage of crosspolarization comparisons to achieve stronger signal strength than single-polarization reflection signals. With further research, including long-term observations and detailed analysis, the proposed method has the potential to enhance performance significantly.

많은 연구에 따르면 Tenebrio molitor은 유충 단계에서 플라스틱을 섭취할 수 있다고 보고되었다. 이 연구의 목적은 T. molitor 유충의 성장과 발달에 발포폴리스티렌 섭취가 미치는 영향을 조사하는 것이다. 밀기울을 섭취한 유충의 성장률은 발포폴리스티렌을 섭취한 유충의 성장률보다 더 좋았고(p < 0.001) 발포폴리스티렌을 섭취한 유 충의 번데기로 전환되는 기간은 밀기울을 섭취한 유충의 번데기로 전환되는 기간보다 더 빨랐다(p < 0.001). 하지만 두 처리구간 생존율은 유의미한 차이가 없었다(p = 0.786). 이 결과에 따르면 발포폴리스티렌을 섭취한 유충은 체중 감소와 짧은 발육기간이 특징이지만 생존하는 것에는 문제가 없었다. 따라서 우리는 T. molitor가 플라스틱 폐기물 의 지속 가능하고 친환경적인 제거를 위한 주요 자원이라는 결론을 내렸다.

The purpose of this study is to analyze effect of Army Risk Assessment System(ARAS) which is used to prevent safety accident in ROK army. Based on prior research, we select 4 indicators which are related to accident prevention effect and analyze the differences before and after ARAS operation for each indicators by using Paired-Samples T-Test. Also, we analyze the correlation between degree of ARAS operation and status of safety accidents of 112 ROK Army units. We conduct an evaluation of each function within the system using IPA method. The results of this study are as follows; All 4 indicators are improved compared to before ARAS operation, and the differences are statistically significant. Also, there is negative correlation between the degree of ARAS operation and the occurrence of safety accidents. So, the operation of ARAS has a positive effect on preventing safety accidents. Finally among the 15 functions of ARAS, 4 functions require improvement. The findings of this study have implications for proposing necessity of computerized system in enforcing Risk Assessment. Also, whether or not operating ARAS is important, but it is also important to operate it well. Lastly, We propose improvement plans for each function to operate it well.

Light is a major external environmental factor that influences the circadian rhythm of photosynthetic organisms and various physiological phenomena, such as growth, maturation, and behavior. The number of light-reaching organisms changes depending on the season and atmospheric conditions, and the intensity and wavelength of light differ depending on the organisms inhabiting the environment. Altered light changes the circadian rhythm of fish, which is controlled by clock genes, such as period 2 (Per2), cryptochrome 1 (Cry1), and melatonin. In this study, we set the zeitgeber time (ZT; 14 light-10 dark, LD) based on the actual sunrise and sunset times and examined Per2 and Cry1 activities, levels of aralkylamine N-acetyltransferase (AANAT), and melatonin in Pholis nebulosa, a drifting seaweed species exposed to irregular light. Per2 and Cry1 levels increased during the daytime and decreased after sunset. The AANAT levels decreased during the daytime and increased during the night. Melatonin concentration was highest around midnight (ZT21, 23:30), but exhibited similar concentrations during the daytime. While the activity of Per2, Cry1, and AANAT levels exhibited a typical circadian rhythm observed in most vertebrates, melatonin concentrations did not show a significant difference between the daytime and nighttime. These findings provide insights into the circadian rhythm patterns of organisms exposed to irregular light environments, such as P. nebulosa, which differ from those of typical fish species.

Haemaphysalis longicornis는 사람과 동물에게 여러 심각한 병원체를 전달하는 주요 매개체로, 한반도에 널리 분포하고 있다. H. longicornis는 Rickettsia spp., Borrelia spp., Francisella spp., Coxiella spp., 그리고 중증열성혈소판 감소증후군 바이러스 (SFTS virus) 등을 매개하는 것으로 알려져 있다. 국내에 서식하는 H. longicornis의 미생물 군집과 관련된 연구는 많이 진행되지 않은 것으로 확인되었다. 이 연구는 한반도 내 다양한 지역에서 채집된 H. longicornis의 미생물군집 다양성을 지역별, 성장 단계 및 성별에 따라 분석하였다. 2019년 6월부터 7월까지 질병관리청 권역별기후변화매개체감시거점센터 16개 지역에서 채집한 H. longicornis의 16S rRNA 유전자 V3-V4 영역을 PCR로 증폭 후 Illumina MiSeq 플랫폼으로 시퀀싱하였다. Qiime2를 활용한 미생물 다양성 분석을 통해 총 46개의 샘플에서 1,754,418개의 non-chimeric reads를 얻었으며, 평균 126개 의 operating taxonmic unit (OTU) 을 식별하여 총 1,398개의 OTU를 확인하였다. 대부분의 지역에서 Coxiella spp.가 우점종으로 나타났으며, 특히 Coxiella endosymbiont는 가장 높은 우점도를 보이며, Coxiella burnetii와 계통 발생 학적으로 유사한 것으로 확인되었다. 이 연구를 통해 분석된 결과는 각 지역의 H. longicornis 미생물군집 데이터 베이스 구축에 활용되었으며, 이를 통해 지역별 미생물군집의 특이성을 식별할 수 있게 하였다. 이는 한반도의 H. longicornis에 의한 질병 전파 연구와 이를 통한 공중보건 개선에 기여할 것으로 기대된다.

Insect cuticular extracellular matrices (ECM) including the eggshell and exoskeleton play vital roles in protecting them from natural environmental stresses. However, these chitinous ECMs must be degraded at least in part during embryonic and post-embyonic molting periods to accommodate continuous growth all the way to the adult stage. In this study we investigated the functions of groups I and II chitinases, TcCHT5 and TcCHT10, in turnover of the eggshell and cuticle in Tribolium castaneum. RNAi and TEM analyses revealed that TcCHT10 is required for digestion of chitin in the serosal cuticle for embryo hatching as well as in the old cuticle during post-embryonic molts including larval-pupal and pupal-adult metamorphosis. However, although TcCHT5 is apparently involved in these vital physiological events, TcCHT10 could substitute for TcCHT5 except during the pupal-adult molting when both enzymes are indispensable to degrade chitin in the old pupal cuticle.

Background: Somatic cell nuclear transfer (SCNT) is a prominent technology that can preserve superior genetic traits of animals and expand the population in a short time. Hematological characters and endocrine profiles are important elements that demonstrate the stability of the physiological state of cloned animals. To date, several studies regarding cloned camels with superior genes have been conducted. However, detailed hemato-physiological assessments to prove that cloned camels are physiologically normal are limited. In this study, We evaluated the hemato-physiological characteristics of cloned male and female dromedary camels (Camelus dromedaries). Methods: Therefore, we analyzed variations in hematological characteristics and endocrine profiles between cloned and non-cloned age-matched male and female dromedary camels (Camelus dromedaries ). Two groups each of male and female cloned and non-cloned camels were monitored to investigate the differences in hemato-physiological characteristics. Results: All the animals were evaluated by performing complete blood count (CBC), serum chemistry, and endocrinological tests. We found no significant difference between the cloned and non-cloned camels. Furthermore, the blood chemistry and endocrine profiles in male and female camels before maturity were similar. Conclusions: These results suggest that cloned and non-cloned camels have similar hematological characteristics and endocrine parameters.

Background: The small intestine plays a crucial role in animals in maintaining homeostasis as well as a series of physiological events such as nutrient uptake and immune function to improve productivity. Research on intestinal organoids has recently garnered interest, aiming to study various functions of the intestinal epithelium as a potential alternative to an in vivo system. These technologies have created new possibilities and opportunities for substituting animals for testing with an in vitro model. Methods: Here, we report the establishment and characterisation of intestinal organoids derived from jejunum tissues of adult pigs. Intestinal crypts, including intestinal stem cells from the jejunum tissue of adult pigs (10 months old), were sequentially isolated and cultivated over several passages without losing their proliferation and differentiation using the scaffold-based and three-dimensional method, which indicated the recapitulating capacity. Results: Porcine jejunum-derived intestinal organoids showed the specific expression of several genes related to intestinal stem cells and the epithelium. Furthermore, they showed high permeability when exposed to FITC-dextran 4 kDa, representing a barrier function similar to that of in vivo tissues. Collectively, these results demonstrate the efficient cultivation and characteristics of porcine jejunum-derived intestinal organoids. Conclusions: In this study, using a 3D culture system, we successfully established porcine jejunum-derived intestinal organoids. They show potential for various applications, such as for nutrient absorption as an in vitro model of the intestinal epithelium fused with organ-on-a-chip technology to improve productivity in animal biotechnology in future studies.

본 연구는 식물공장에서 아쿠아포닉스와 수경재배에서 재배된 딸기의 무기양분 흡수율, 생육, 수량을 비 교하고자 수행되었다. 양어는 비단잉어(Cyprinus carpio) 12마리를 수조(W 0.7m × L 1.5m × H 0.45m, 472.5L)에 367.5L 물을 채운 후 입식하였고 5.44kg·m-3 밀도로 사육하였다. 딸기 모종 30개체는 펄라이트를 채운 포트에 정식 하여 아쿠아포닉스 시스템 베드(W 0.7m × L 1.5m × H 0.22m)에 장착하였고, 모종 30개체는 네트포트에 정식한 후 담액수경(DFT)시스템 베드(W 0.7m × L 1.5m × H 0.22m)의 아크릴판(140 cm × 60 cm, Ø80 mm)에 장착하였다. 재 배기간 동안 아쿠아포닉스 수조액의 pH와 EC는 각각 4.3－6.9, 0.32－1.14dS·m-1 수준이었고, 수경재배는 각각 5.1 －7.5, 1.0－1.8dS·m-1이었다. 아쿠아포닉스 수조액의 NO3-N와 NH4-N 농도는 수경재배보다 각각 약 3.6, 2.2 me·L-1 높았다. P, Ca, Mg, S 농도는 수경재배보다 각각 약 0.76, 3.1, 0.8, 0.9me·L-1 높았으며, K와 Fe는 각각 약 0.8me·L-1, 0.5mg·L-1 낮았다. 딸기 잎의 무기이온 함량은 두 재배 처리 간 유의차가 없었으며 엽내 K 함량은 적정 범위를 보였 다. 정식 후 58과 98일 사이에 아쿠아포닉스에서 재배된 딸기의 T-N와 P 흡수율은 수경재배보다 각각 1.5, 1.9배 높 았고 K 흡수율은 유의차가 없었다. 개체당 과실수는 아쿠아포닉스에서 수경재배보다 유의하게 많았으며, 상품과 생체중, 건물중, 과실의 과장과 과폭은 수경재배에서 아쿠아포닉스보다 높았다. 결과를 종합하면, 아쿠아포닉스에 서는 수조액의 물고기 배설물과 먹이 잔여물에 의한 고체 입자의 비료성분을 지속적으로 가용하여 활용한다는 것 을 알 수 있다.

This research investigated the preparation of activated carbon derived from Krabok (Irvingia malayana) seed shells to improve the quality of crude glycerol obtained during biodiesel production. The activated carbon was prepared using a dry chemical activation method with NaOH, utilizing an innovative biomass incinerator. The results revealed that the resulting KC/AC-two-step exhibited favorable physicochemical adsorption properties, with a high surface area of 758.72 m2/g and an iodine number of 611.10 mg/g. These values meet the criteria of the industrial product standard for activated carbon No. TIS 900-2004, as specified by the Ministry of Industry in Thailand. Additionally, the adsorption efficiency for methylene blue reached an impressive 99.35 %. This developed activated carbon was then used to improve the quality of crude glycerol obtained from biodiesel production. The experimental results showed that the KC/AC-two-step increased the purity of crude glycerol to 73.61 %. In comparison, commercially available activated carbon (C/AC) resulted in a higher crude glycerol purity of 81.19 %, as analyzed by the GC technique. Additionally, the metal content (Zn, Cu, Fe, Pb, Cd, and Na) in purified glycerol using KC/AC-two-step was below the standards for heavy metals permitted in food and cosmeceuticals by the Food and Drug Administration of Thailand and the European Committee for Food Contact Materials and Articles. As a result, it can be inferred that Krabok seed shells have favorable properties for producing activated carbon suitable as an adsorbent to enhance crude glycerol purity. Furthermore, the improved crude glycerol from this research has potential for various industrial applications.