Background : Heat stress induced from high temperature are known to crucially affecting on physiological properties and yield in Cnidium officinale. Methods and Results : The effect of foliar application of mixture including a urea, ascorbic acid and calcium chloride on high temperature injury of Cnidium officinale. Photosynthesis and leaf temperature in Cnidium officinale were investigated after cultivating for 24 hours at 35℃. Net photosyntheis rate, transpiration was measured at 1,000 μmol m-2 s-1 of photon flux density and leaf temperature was analyzed by thermal image. Net photosyntheis rate, stomatal conductance and transpiration rate in mixture traetment were 2 times of higher than in control. Water use efficience was not different significantly. Leaf temperature was lower in mixture treatment (25.3℃) than in control (29.0℃). Conclusion : This result show that foliar application of urea, ascorbic acid and calcium chloride was reducing a high temperature injury through a improving photosynthetical capacity and decreasing leaf temperature of Cnidium officinale.

Background : Management of air temperature are known to primarily affecting on physiological properties and yield in plant. Methods and Results : The effect of air temperature on characteristics of photosynthesis and chlorophyll fluorescence in Cnidium officinal were investigated using growth chamber after cultivating for 24 hours under controlled condition. Net photosyntheis rate, transpiration was measured at 1,000 μmol m-2 s-1 of photon flux density and chlorophyll fluorescence was analyzed by OJIP method. Net photosyntheis rate was highest in treatment of 25℃. Although transpiration rate was lowest, water use efficience was also in treatment of 25℃. Stomatal conductance was mainly influenced from ambient climatric factors such as vapor pressure deficit. As results of chlorophyll fluorescence by OJIP analysis, maximum quantum yield (Fv/Fm) of photosystem II (PSII), PIabs and the relative activities per reaction center such as ABS/RC, DIo/RC were not changed at air temperature. Therefore, elevated air temperatue during short term influence the dark reaction in photosystem through controlling a water use efficience and transpiration. Conclusion : This result show that 25℃ of air temperature may be a adequate temperature to improving the efficiency of photosynthesis in Cnidium officinale.

Background : Corrosion is one of the most devastating problems faced by most industries. Mild steel has played a vital role in various fields due to the excellent mechanical properties of mild steel such as low density, high strength-to-weight ratios, good environmental stability, high thermal conductivity, and corrosion resistance. Methods and Results : The total phenolic contents (TPC) and total flavonoid contents (TFC) of the methanolic extract of C. grandiflora and R. verniciflua leaf have been examined, and its corrosion inhibition performance was investigated by weight loss and electrochemical impedance spectroscopy (EIS) and polarization measurements. The surface morphology of mild steel was analyzed by scanning electron microscopy (SEM) equipped with energy dispersive X-ray spectroscopy (EDX) and by atomic force microscopy (AFM). The percentage composition of polyphenolic compounds was found to be higher in C. grandiflora and R. verniciflua plant extracts, and it was proved to be a superior, eco-friendly, and anti-corrosive inhibitor for mild steel in 1M of H2SO4. The Tafel polarization studies indicate that the plant extract is a mixed-type inhibitor. Scanning electron microscopy/energy -dispersive X-ray spectroscopy (SEM-EDS), and atomic force microscopy (AFM) studies confirmed the formation of a protective film on the metal surface. The corrosion inhibition of the C. grandiflora and R. verniciflua plant extracts was characterized by Fourier transform infrared (FT-IR), UV-visible spectra, and wide-angle X-ray diffraction (XRD) studies; these show the strong interaction between the metal surface and the inhibitor. Conclusion : The methanolic extract was prepared the two different plants like C. grandiflora, and R. verniciflua was studied the corrosion inhibition on the mild steel specimen in acidic medium through various methods involving weight loss measurements, EIS, and potentiodynamic polarization. The results shows that the C. grandiflora, and R. verniciflua plant extracts illustrate an effective corrosion inhibitor for mild steel with good anticorrosion properties in acidic environmen

Background : Recently, ginseng (Panax ginseng C.A. Meyer.) berry has been used as a health-promoting supplements. Also, Mulberries (Morus alba L.) fruit have been used in traditional herbal medicine to treat and prevent diabetes. In this study, we measured the cytotoxicity after fermentation of the extracts in Panax Ginseng Berry and Mulberry Fruit. Methods and Results : The extracts were prepared by decoction for 3 hours in distilled water (100 g/L). The dried extract was then dissolved in phosphate-buffered saline (PBS) in preparation for use. Cell viability was examined by an MTT assay. RAW 264.7 cells were seeded at 1 × 104/mL densities in 96-well plates. Each grouping had a non-treated group as the control. The extracts were added to each well and incubated for 24 hours at 37°C and 5% CO2. The MTT solutions (5 mg/mL) were added to each well, and the cells were cultured for another 2 hours. The supernatant was then discarded, and 100 μL of dimethyl sulfoxide was added to each well. The optical density was read at 540 nm. Conclusion : Probiotics and prebiotics modulate the composition of human and domestic animal gut microbiota. The beneficial effects may result from suppression of harmful microorganisms or stimulation of organisms which contribute in a positive way to the nutrition and health of human and domestic animal. Recently, fermentation using microorganisms for the production of more effective compounds has been extensively studied. In particular, the novel pharmacological effects of a new compound generated by fermentation have been reported. Some previous studies have demonstrated that Fermented herbal medicine extract showed better bioactivity than normal herbal Plants extract when used at the same concentration.

Background : Angelica dahurica Bentham et Hooker, Angelica gigas Nakai, Ostericum koreanum Maximowicz and Peucedanum japonicum Thumberg　are a major medicinal plant in north Geungbuk province. Using medicinal plants are impotant it`s ingredient. Dry condition and stroage method are not standard manual. The ingredient variation of dry condition and stroage method were not researched. Methods and Results : Using plant material were cutivated on Gyongsangbukdo Bonghwa area. It were studied ingredient variation after dry and storage condition by HPLC methods. Major ingredient of Angelica gigas Nakai are decurusin, decurusinangelate. Heated air bulk dry get more decursin than natuarl dry and decurusinangelate of natural bulk dry was higher than heated air bulk dry. Major ingredient of Ostericum koreanum Maximowicz are imperatorin and isoimperatorin.. Imperatorin of Ostericum koreanum was highest peak on 50℃ heated-air dry after plastic bag sorage and isoimperatorin was highest peak on 40℃ heated-air dry after mountain cultivation. Imperatorin is a major ingredient Angelica dahurica Bentham et Hooker. Heated air bulk dry get more decursin and decursinangelate than natuarl dry and small heated-air dry. Peucedanol-7o_glucoside is a major ingredient Peucedanum japonicum Thumberg. Natural bulk dry get more peucedanol-7o_glucoside than heated-air bulk dry. Conclusion : Ingredient of Angelica dahurica Bentham et Hooker, Angelica gigas Nakai, Ostericum koreanum Maximowicz are different under various cutivation, drying method, storage. Diffent Ingedients of Angelica gigas Nakai, Ostericum koreanum Maximowicz were not accord it’s optical conditon.

Background : Schizandra chinensis Baillon have five tastes and lately it is using a beverage broadly. Schizandra chinensis is one of the top producing medicinal plant in Korea. Mungyeong of Gyongbuk province produce almost of Schizandra chinensis. Maturity of Schizandra chinensis get 3 years and proliferation of Schizandra chinensis was not a manual. It is needed that a new cultivar has a big fruit and high quality chracteristics using processed food and beverage. Methods and Results : 105 lines of Schizandra chinensis were collected on Mungyeong, Yeongwol, Jinan. It were studied it’ characteristics especially it’s fruit trait. Fruit traits of Schizandra chinensis were researched on fruit length, fruit weight, maturity, number of fruit, male and female ratio, powdery mildew. Fruit length of Schizandra chinensis is relation of fruit weight. It were founded 15 lines of long fruit length. 5 lines were studied high fruit weight and it’s weight were 32 to 41g. Number of fruit has relation with fruit weight and high fruit weight gets many fruits. it’s numer of fruits were 3 to 41. Male and female ratio were very impotant characteristic. High level of female ratio has quantity of fruit. High level of female ratio were founded 2 lines. Finally It was selected 3 good breed lines of Schizandra chinensis. Conclusion : 105 lines of Schizandra chinensis Baillons were collected on Mungyeong, Yeongwol, Jinan. It were founded 15 lines of long fruit length and 5 lines were studied high fruit weight. High level of female ratio were founded 2 lines. 3 good breed lines of Schizandra chinensis were selected.

Background : The roots of Curcuma longa Radix is used to make herbal medicine, which is registrated in the Korean Pharmacopoeia. This species is a perennial herb, and is distributed throughout tropical and/or sub tropical regions. High temperature and humidity with small difference between daytime and nighttime is the finest environment condition in this species. Recently, demand for C. longa has been expanded to cultivation area as central region from southern region. Therefore, this study aimed to investigate the effect of harvest time on the growth, yield characteristics and main composition contents in C. longa. Methods and Results : Curcuma longa rhizomes were harvested from farmland in Jindo, Jeollanam-do in end October, early November, middle November, end November, early December, middle December and end December 2015. Harvested rhizome were dried in hot air drier, and it was investigated as growth characteristics and yield. Contents of curcumin, demethoxycurcumin, bisdemethoxycurcumin was analyzed from dried powder of rhizome. The analysis column was used to Phenomenex, Kinetex 2.6um F5 100A 100*2.1mm. 10 mg of standard was taken, 80% MeOH was added to dissolve in 10 ㎖ flask. 0.5, 1, 2, 4 ㎖ was taken from dissolved solution and then was added to 10 ㎖ of 80% MeOH. It was filtered through 0.45 ㎛ filter and then 5 μℓ was taken to make standard solution. Analytical calibration curve was measured to diluted solution within sample concentration. 2 g of ground sample was taken, 40㎖ of 80% MeOH was added to extract by ultrasound sonication during 60 minutes and then it was filtered through 0.2 ㎛ filter to analyze component content. Conclusion : The root weight significantly increased from 376.1 g in end October to 695.9 g in end December as 1.8 double. Curcumin contents differed significantly among harvest times in end November and early December. Demethoxycurcumin increased from early November to end November, and Bisdemethoxycurcumin increased to the early November.

The GA application on grapevines induces parthenocarpy, fruit set without fertilization, and the inhibition of pollen tube growth. But the molecular mechanism underlying this inhibition is not understood. Similar defective pollen tube growth within the transmitting tract has been reported in the mutant of GABA transaminase (GABA-T), referred to as pollen-pistil-interaction2 (pop2) in Arabidopsis. In spite of the similarity of pollen tube growth inhibition observed in GA-applied grapevines with that of pop2, only the effects of GABA on stress responses in grapevines have been reported. In present study, transcriptional changes of Vitis GABA metabolic genes, together with changes in GABA levels with or without GA application were analyzed to define how GA application restrained the pollen tube growth in grapevines. A GA solution (Dongbu, Seoul, Korea) at 100 ppm was onto inflorescence clusters 14 days before full bloom (DBF) and clusters were harvested at 0, 1, 2, 4, 7, 9, 12, 14, 16, and 19 days after GA application. Harvested inflorescence samples were immediately frozen in LN2 and extracted RNA and amino acid. The GABA contents were analyzed using high-performance liquid chromatography (Agilent 1100 HPLC, Agilent Technologies, Inc., Santa Clara, USA) equipped with a C18 column (4.6 mm×150 mm, 3.5 μm/VDS optilab, Berlin, Germany), according to the manufacturer’s instructions. Without GA application, the simultaneous high expressions of VvGAD1, VvGAD4 and VvGABA-T2 during 10 to 5 days before full bloom (DBF) showing the activation of GABA metabolism. But the contents of GABA were low before 2 DBF, and it peaked only at near full bloom when expression levels of VvGABA-T2 remained low. After GA application, the contents of GABA were constant during 10 to 5 DBF, although transcription levels of both VvGAD1 and VvGABA-T2 rapidly declined less than 30% of the levels observed without GA application. However, the GABA levels increased more than 2-fold only at near full bloom, compared to those without GA application, and at that time, expression levels of VvGAD1 up-regulated more than 3-fold and those of VvGABA-T2 kept low. But other amino acid contents did not show significant changes. In case of VvSSAHDs, their transcriptional changes with or without GA application were not correlated with GABA levels. These results indicates that GABA levels before pollination is tightly regulated, but GA application alters the GABA-shunt to accumulate excess GABA more than needed for proper pollen tube growth at full bloom. Gibberellin application alters the GABA-shunt to accumulate excess GABA resulting in inhibition pollen tube growth in grapevines.

‘Clara’ 품종은 국립원예특작과학원에서 2005년도에 육성한 포인세티아 품종으로 초장이 작고 컴팩트한 수형으로 포엽의 형태는 난형이며 엽맥 사이 주름의 정도는 약하다. 포엽의 길이와 폭, 잎몸의 길이와 폭, 엽병의 길이는 짧으며 단일처리 후 약 9주일이 경과하면 충분히 착색되어 출하가 가능한 품종이다. 2008년 5월과 10월 ‘Clara’ 품종의 캘러스가 형성된 삽수에 100Gy의 감마선을 24시간 동안 처리하여 유기한 돌연변이를 이용하여 2010년과 2013년에 국립원예특작과학원에서 ‘Clara Pink’와 ‘Clara White’품종을 육성하였다. 이 두 품종들은 포엽의 색이 완전히 변한 변이주를 선발하여 계통화 하였으며 2008년 5월에 감마선을 처리한 삽수들 중 포엽의 색이 분홍색인 변이지를 선발하여 2009년에 2차에 거쳐 특성검정을 실시하였다. 2010년에 ‘원예 D5-2’를 육성한 후 3차 특성검정과 품종평가회를 실시하여 최종선발하였으며, 농작물 직무육성 신품종선정위원회를 거쳐 ‘Clara Pink’로 명명하였다. 2005년 10월에 감마선을 처리한 삽수들중에서는 포엽의 색이 연황색인 변이주를 선발하여 계통화 하였으며, 2012년과 2013년에 1,2차 특성검정을 실시하였다. 2013년에 ‘원예 D5-34’를 육성하여 특성검정과 품종평가회를 실시하고 농작물 직무육성신품종선정위원회를 거쳐 ‘Clara White’로 명명하였다. ‘클라라 핑크’와 ‘클라라 화이트’ 두 품종 모두 ‘클라라’ 품종과 같은 소형으로 적심하지 않은 상태에서도 분지가 많이 발생하는 컴팩트한 수형이다. 포엽의 형태는 결각이 없는 난형이며, 엽맥 사이에 약한 주름이 있고 단일처리 후 약 9주일 경과하면 완전히 착색된다. 잎몸의 모양은 난형이며, 엽병의 길이는 짧다. 그러나 ‘클라라 핑크’와 ‘클라라 화이트’ 품종의 포엽과 엽맥의 안토시아닌 발현에 차이를 나타내었다. ‘클라라 핑크’ 의 포엽은 분홍색이며, ‘클라라 화이트’의 포엽은 연황색이다. 또한 ‘클라라 핑크’ 와 ‘클라라 화이트’ 품종은 ‘클라라’ 품종과 비교해서 잎자루 윗면의 안토시아닌 발현 정도가 약하였으며, 잎몸 윗면의 가운데 맥의 색이 ‘클라라’ 품종은 녹색과 빨강색이 함께 발현되었으나, ‘클라라 핑크’ 와 ‘클라라 화이트’ 품종은 녹색만 발현되었다.

Gibberellic acid (GA) is a well-characterized plant hormone, which plays a critical role in various plant growth and development. including stem elongation, floral indcution and seed development. GA is known to cause enlargement of ripening fruits and, especially in grapevines, GA shows a unique function: the induction of seedlessness in seeded grape varieties. However, despite extensive previous studies about GA, there has been no clear verification of the mechanism that induces seedlessness in grapes. To understand how GA treatment results in artificial parthenocarpy of seeded grapes at molecular levels, we analyzed transcriptional changes in seeded grapes with and without GA application in various inflorescence developmental stages using RNA-seq. At 14 days before flowering (DBF), seeded grapes were treated with 100 ppm GA and clusters were collected at three developmental stages: 7 DBF, full bloom, and 5 days after flowering (DAF). Of a total of 28,974 genes that were mapped to grape genome reference sequences, 7,013 and 9,064 genes were up- and down-regulated, respectively, in the GA-treated grape as compared to the non-GA-treated control at 7 DBF, full bloom, and 5 DAF. Clustering analysis revealed that these genes could be grouped into 9 clusters with different expression patterns. We also carried out functional annotation based on gene ontology categories. There were significant differences in the expression of the GA and auxin-related gene families. These findings expand our understanding of the complex molecular and cellular mechanisms of GA-induced parthenocarpy of grapes and provide a foundation for future studies on seed development in grapevines.

Twenty two common millet (Panicum miliaceum L.) varieties collected from Korea, China and Russia were investigated for their phylogenetic relationship using 5S ribosomal DNA sequences with a hope to provide the basic information on their exact origin. Sequences of 5S rDNA were isolated by PCR. The primers, 5s-rRNA1 and 5s-rRNA2, were designed to isolate the complete NTS. Genomic DNA amplification produced two fragments with different length, 900 bp and 400 bp fragments, confirming the presence of two types of 5S rDNA repeats that differed from each other in the length of the NTS region. Amplified DNAs of 400 bp fragment were subcloned and used for further investigation. The obtained NTS sequences ranged from 200 to 300 bp and homology of sequences among plant materials was much higher than long repeat. CLUSTALW multiple aligment of 5S rDNA sequences from 22 different common millets revealed the clear difference by their origin. And critically different areas with insert or deletion were also confirmed. Those sequence difference seems to be used for discrimination of cultivars from different origin and use as molecular markers for origin identification. In phylogenic tree construction, the clear classification was shown where the genotypes from China and Russia is positioned together and stay away from domestic genotypes.

나물용 녹두 신품종 '주현'은 금성녹두와 VC1834-4-B-2-B-3B-B를 인공교배하여 2009년에 육성되었다. '주현'의 생육습성은 직립, 잎 모양은 심장형, 백축색은 녹색, 성숙기에 꼬투리는 검고 곧으며, 종피는 광택이 없는 녹색이다. 개체당 협수는 26개로 표준품종인 '어울녹두'보다 8개 많고, 녹두나물 수율은 표준품종보다 18% 많다. '주현'은 바이러스, 갈색무늬병, 흰가루병 및 도복에 대한 포장저항성은 강하고, 평균수량은 1.84 MT/h

청보리 신품종 '유호'는 까락이 없어 가축 기호성이 높지만 수확 시 곡실의 탈립이 많아 수량 및 영양적 손실이 큰 기존 삼차망 품종 '유연'의 단점을 개선하기 위하여 1999년에 내탈립 삼차망인 수원339호를 모본으로 하고, 보리호위축병에 강하고 총체적성에 좋은 수원355호를 부본으로 교잡하여 계통육종법에 의하여 제반 특성이 우수한 'SB992047-B-B-B-6-2' 계통을 선발, 생산력검정시험과 지역적응시험을 거쳐 2008년에 육성되었다. 그 특성을

신품종 '영한'은 병해 및 도복, 한해 등 재해에 강해 수량이 많고 품질이 우수한 청보리 품종을 육성하기 위하여 1997년에 조숙, 내도복, 내병, 다수성 계통인 'YB3433-3B-5'와 조사료 품질이 좋고 수량이 많은 'YB3135-3B-2-3' 사이의 F1에 'YB3135-3B-2-3'를 여교잡하고 계통육종법에 의하여 제반특성이 우수한 'SB971305-B-B-B-B-4-4' 계통을 선발, 생산력검정시험과 지역적응시험을 거쳐 2008년에 육성되었다