최근 페이스북 광고의 마케팅 가치가 증가됨에 따라, 기업들은 브랜드 및 상품 홍보를 위해 성공적인 페이스북 광고제작 방안에 관심을 두고 있다. 이에 따라 본 연구에서는 페이스북 광고에 대한 사용자의 시선과 관심에 영향을 미치는 요인을 도출하고, 효과적인 페이스북 광고 콘텐츠의 시지각 구성요소들의 특성을 규명하였다. 먼저 페이스북 광고에 대한 사용자의 반응에 영향을 미칠 수 있는 두 가지 변인 요인을 설정하였다: 페이스북 광고 콘텐츠 포맷 (Text, Text in Image, Movie)과 광고 제품 속성(관여도, 이성/감성). 이에 대한 이론적 논의를 바탕으로 시선추적과 설문조사를 접목하여 실험 연구를 진행하였다. 페이스북 광고 콘텐츠 포맷(Text, Text in Image, Movie)과 광고 제품 속성(관여도, 이성/감성)이 사용자 반응(시지각 인지, 사용자 구매 행동 반응)에 미치는 효과를 분석하였다. 그 결과, 광고 콘텐츠 포맷에 따라 차별화된 사용자의 시지각 인지 패턴과 구매 행동 반응이 나타났다. 반면, 광고 제품속성의 경우 사용자의 구매 행동 반응에만 영향을 미치는 것을 알 수 있었다. 마지막으로 본 연구의 주요 연구 결과를 토대 로 광고 콘텐츠 제공자 혹은 개발자들을 위한 효과적인 SNS 광고 제작 가이드라인을 제안할 수 있었다.

This study investigated the effects of L-carnitine (LC) and nicotinic acid (NA) on sperm viability during liquid storage at 18℃ in miniature pigs. 10 μM LC and 30 mM NA, combined LC and NA (LN) were treated in fresh semen for 3, 7, and 10 days. In results, sperm survival increased in NA- and LN-treated semen on 7 and 10 days (p<0.05), mitochondrial integrity of live sperm increased in LN-treated semen on 7 days (p<0.05), but not NA-treated semen. In addition, we examined the acrosome reaction of sperm in miniature pigs. LC and NA did not influence on acrosome reaction of boar sperm. In conclusion, LC and NA effectively maintained the viability and quality of sperm during long-term storage in miniature pigs, suggesting that the combined LN may be useful for improving the semen extender for long-term liquid storage in pigs.

The objective of this study was to investigate the efficiency of nicotinic acid during in vitro fertilization (IVF) in frozen-thawed bull sperm . The ejaculated semen was diluted with Triladyl containing 20% egg-yolk and cryopreserved in liquid notrigen. The frozen sperm was thawed for 45 seconds in the 38℃ water bath. Sperm was diluted with IVF medium (Bovine-Oviduct medium; BO) containing 0, 15, 30 and 60 mM nicotinic acid (NA), which were incubated at 39℃, 5% CO2 for 0, 0.5, 1, 2 and 4h. The characteristics of frozen-thawed sperm were estimated with SYBR14/PI double staining for viability, FITC-PNA/PI for outer acrosomal membrane damage and Rhodamine123/PI for mitochondrial integrity using flow cytometry. And the sperm ability was analysed by Coomassie brilliant blue (CBB) staining for acrosome reaction state and Rose bengal staining for abnormality. Acrosome reaction and abnormality were analyzed using a microscope. In results, sperm viability was significantly higer in 30 mM group than 0 and 15 mM groups at 1 and 2 h (p<0.05). Outer acrosomal membrane damage was significantly lower in 30 mM group than 0 and 15 mM groups at 1, 2 and 4 h (p<0.05). And mitochondrial integrity was significantly higher in 30 mM group than 0 and 15 mM groups at 2 and 4 h (p<0.05). Also, acrosome reaction was significantly lower in 30 mM than 0 and 15 mM groups at 1 and 2 h (p<0.05) and abnormality was lower NA groups than 0 group at 1 h (p<0.05). In couclusion, we suggest that using the thawing medium containing NA for sperm dilution can be benefical for IVF in bulls

The objective of this study was to evaluate the efficiency of sperm cryosurvival in boar sperm separated by Percoll containing antioxidant enzymes. The boar semen was collected into a pre-warmed (37℃) thermos bottle by gloved-hand method and was separated by 65% Percoll with superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) before freezing. The frozen sperm was thawed at 38.5℃ for 45 sec in water-bath for sperm characteristic analysis. The sperm were estimated with SYBR14/PI double staining for viability, FITC-PNA/PI double staining for acrosome reaction, Rhodamine123/PI double staining for mitochondrial integrity and were analyzed using flow cytometry. In results, sperm viability, acrosome reaction and mitochondrial integrity were improved in separated sperm groups compared with unseparated sperm by Percoll (UP) group. Especially, viability was significantly higher in sperm separated by Percoll containing 400 IU CAT group compared with other groups (P<0.05). And acrosome reaction was decreased in sperm separated by Percoll with 300 IU SOD, 400 IU CAT and 0.5 mM GSH groups compared with other groups, however, there were no significantly difference mitochondrial integrity among sperm separated by Percoll with antioxidant enzymes. In conclusion, we suggest that use of Percoll containing antioxidant enzymes for sperm separation will be beneficial for sperm cryopreservation in pigs.

This study investigated whether people experience a correspondence between color hue-tone and the main characters’ emotions in the 2012 British musical drama film, Les Misérables through three practical experiments. Six screen images, which represent the characters’ different emotions (Parrot’s six primary types including love, joy, surprise, anger, sadness, and fear) were selected. For each screen image, participants were asked to judge the degree of the character’s dominant emotions evoked from 17 varied screen images, which consisted of original chromatic and achromatized images as well as 15 color-filtered images (5 hues X 3 tones of the IRI color system). These tasks revealed that a chromatic color scheme is more effective to deliver the characters’ positive emotions (i.e. love and joy) than an achromatic one. In addition, they proved that the hue and tone dimensions partially influence the relationships between the character emotions and colors.

The objective of this study was to investigate the efficiency of nicotinic acid on sperm cryosurvival and fertilization ability in frozen-thawed boar semen. Boar semen was collected by glove-hand method and was frozen using freezing solution treated to 0, 5, 10 and 20 mM of nicotinic acid. The frozen sperm for sperm characteristic analysis was thawed such as viability, acrosome reaction, and mitochondrial integrity. The frozen-thawed sperm was estimated by SYBR14/PI double staining for viability, FITC-PNA/PI double staining for acrosome reaction and Rhodamine123/PI double staining for mitochondrial integrity using a flow cytometry. The embryo was estimated in vitro development and DCFDA staining for reactive oxygen species assessment. As results, frozen-thawed sperm viability was significantly higher in 5 and 10 mM (61.1 ± 1.5%, 64.7 ± 2.0%) of nicotinic acid than other groups (0 mM, 52.1 ± 2.3%; 20 mM, 47.8 ± 5.1%, P<0.05). The live sperm with acrosome reaction was significantly higher in 5 and 10 mM of nicotinic acid (26.1 ± 1.8%, 24.9 ± 1.5%) than other groups (0 mM, 35.3 ± 0.8%; 20 mM, 36.5 ± 1.9%, P<0.05). The live sperm with mitochondrial integrity was significantly higher in 5 and 10 mM (84.2 ± 3.6%, 88.4 ± 2.3%) of nicotinic acid than other groups (0 mM, 77.3 ± 4.4%; 20 mM, 73.3 ± 3.6%, P<0.05). Blastocyst rate of in vitro development was significantly higher in 10 mM (17.0 ± 1.3%) of nicotinic acid than other groups (0 mM, 9.4 ± 0.5%; 5mM, 12.6 ± 0.8%; 20 mM, 5.0 ± 1.0%, P<0.05). Moreover, total cell number was higher in 5 and 10 mM (53.6 ± 2.9%, 57.9 ± 2.8%) of nicotinic acid than other groups (0 mM, 41.0 ± 1.4%; 20 mM, 23.2 ± 2.8%, P<0.05). Hydrogen peroxide in embryos was lower in 5 mM nicotinic acid (0.7 ± 0.1%) than other groups (0 mM, 1.0 ± 0.1%; 10mM, 0.9 ± 0.0%; 20 mM, 1.4 ± 1.0%, P<0.05). In conclusion, nicotinic acid-treated semen improves cryosurvival and quality of spermatozoa. Also, the fertilized oocytes with nicotinic acid improve quality of embryo and blastocyst formation.

The objective of this study was to investigate the changes of oxidative stress and antioxidant enzyme during in vitro development with washing culture oil in porcine embryos. During the culture, the four types of culture oil such as paraffin oil with or without washing and mineral oil with or without washing were examined. The oil was washed with PZM-3 during 7 days and collected oil only. The embryos were stained with CellTrackerTM Red, DC-FDA and Hoechst 33342 to confirm the effects of the oil. As a results, Cleavage rates and total cell number were no difference among the four oil groups. However, ≥16 cell embryos were significantly different in fore type oil treat-ment and blastocyst rate was significantly higher washing paraffin treatment than in other group(p<0.05). Also, the expression of free radical were lower in washing paraffin oil than in other groups (p<0.05). On the other hand, the expression of glutathione were not significant different among paraffin oil with or without washing and mineral oil with or without washing, however washing paraffin oil and washing mineral groups were higher than other treat-ment groups. In conclusion, the washing oil was expected with positive effects on in vitro development in porcine embryos.

The objective of this study was to investigate the relationship between fertility and protein pattern change using in vitro fertilization, analysis of sperm characteristics and two-dimensional gel electrophoresis in different pig types. In results, the viability and mitochondria integrity of sperm were higher significantly (p<0.05) but the portions of ac-rosome reaction was lower significantly (p<0.05) in Duroc and F1 (potbellied×PWG miniature pig) than PWG minia-ture. On in vitro fertilization to investigate fertility, the fertility of F1 semen war higher significantly (p<0.05) than in Duroc and PWG miniature pig. On the other hand, protein patterns showed similar function among the different boar semen. Especially, the heat shock 70 kDa 1-like and G patch domain-containing protein 4 were significantly (p<0.05) higher expressed in F1 than in Duroc and PWG miniature pig. The proteins associated with mitochondria in Duroc were significantly (p<0.05) higher expressed than in F1 and PWG miniature pig. The developmental rates to blastocyst stage of oocytes fertilized with sperm of F1 pig were significantly (p<0.05) higher than in PWG miniature pig. However, phosphoglycerate kinase 2 and zinc finger protein 431 were significantly (p<0.05) higher expressed in PWG miniature pig than in F1 and Duroc pigs. In conclusion, the results of the present study indicate that different proteins were expressed in different pig types, and were associated with a sperm functions and embryo develop-ment.

This study is carried out in order to develop food materials for the promotion of rice consumption. In this study, we researched the physicochemical properties of extruded rice flour (ER) and gelatinization popped rice flour (GPR) by the extrusion process and by the popped method with milled rice and brown rice. The extrusion process used a couple of screw extruders. The extrusion parameter was kept constant at a feed moisture content of 25%, barrel temperature of 120℃ and screw speed of 400 rpm. GPR was prepared by batch popping machine after gelatinization of rice. In order to evaluate the physicochemical properties of ER and GPR, as well as the gelatinization rate, water soluble index (WSI), water adsorption index (WAI), DPPH radical scavenging activity and total dietary fiber content, the color value was carried out. The gelatinization rate was similar to 71.1～73.8%. Further, the results of WSI and WAI were increased at ER and GPR compared to the raw materials; ER was also higher than GPR. DPPH radical scavenging activity and total dietary fiber content showed a tendency to reduce after extrution and popping. As a result of the color value, the L value of ER decreased more than the raw materials, whereas GPR increased at a and b values.

파김치에서 분리한 마늘 내성 유산균인 Lactobacillus plantarum TJ-LP-002 균주의 균체생육과 항균활성에 영향을 미치는 배양조건 및 배지조건을 조사하였다. 선정 유산균의 배양 상등액 내에는 acetic acid, citric acid, lactic acid, tartaric acid와 같은 유기산이 존재하였고, 배양 중에 lactic acid와 acetic acid의 생성이 크게 증가하는 것으로 확인되었다. 단백질분해효소를 비롯한 각종 효소 처리에 의해 항균활성이 소실되지 않아, 선정 유산균이 생산하는 항균활성은 단백질성 물질이 아닌 산 생성에 의한 작용일 것으로 판단되었다. 항생제와 생균제의 병용 가능성을 확인하기 위하여 선정 유산균의 항생제 감수성을 조사한 결과, neomycin sulfate, spectinomycin dihydrochloride, lincomycin hydrochloride에 내성을 나타내었고, streptomycin sulfate에는 감수성을 나타내었으며, ampicillin trihydrate, chloramphenicol, erythromycin, tetracycline hydrochloride, kanamycin sulfate에는 중간 내성을 나타내었다. L. plantarum TJ-LP-002는 배양온도 30oC, 초기 pH 7.0, 24시간의 배양 조건에서 최적의 균체생육과 항균활성을 나타내었으며, 탄소원은 glucose 3%(w/v), 질소원은 yeast extract 3%(w/v) 첨가 시에 균체생육과 항균활성이 높게 나타났다. 무기염류는 manganese sulfate와 ammonium citrate가 항균활성에 많은 영향을 주는 것으로 나타났으며, 각 성분을 단독 첨가하는 것보다 혼합 첨가하는 것이 더 우수한 영향을 나타내는 것으로 확인되었다.

마늘의 항균력에 저항성을 갖는 유산균을 분리하기 위해서 마늘 추출물이 첨가된 배지에서 생육하는 112 균주를 김치, 젓갈, 장아찌류로부터 분리하였다. 이 중에서 14균주에 대해서 내산성 및 내담즙성 그리고, 가축 병원성 세균인 Salmonella choleraesuis, Escherichia coli, Staphylococcus aureus, Shigella flesneri에 대해 항균력을 조사한 결과, 파김치 유래의 P'GW50-2 균주가 가장 우수한 특성을 지니고 있어 생균제로 이용 가능한 균주로 선발하였다. 선발된 균주는 16S rRNA 유전자의 염기서열 분석결과 Lactobacillus plantarum으로 동정되었고 L. plantarum TJ-LP-002으로 명명하였다. L. plantarum TJ-LP-002는 Bacillus cereus, S. aureus, Clostridium perfringens와 같은 Gram 양성균과 Aeromonas hydrophila, E. coli, Pseudomonas, Salmonella, Shigella와 같은 Gram 음성균에 대해서 비교적 넓은 항균활성을 나타내었다.

In this study, solid-liquid separation conditions for coagulation and sedimentation experiments using inorganic coagulant (aluminum sulfate and Poly-Aluminum Chloride (PAC)) were optimized with brine wastewater discharged by the epoxy-resin process. When the turbidity and suspended solid (SS) concentration in raw wastewater were 74 NTU and 4.1 mg/L, respectively, their values decreased the lowest in a coagulant dosage of 135.0 - 270.0 mg Al3+/L. The epoxy resin was re-dispersed in the upper part of wastewater treated above 405.0 mg Al3+/L. The removal efficiencies of turbidity and SS via dosing with aluminum sulfate and PAC were evaluated at initial turbidity and SS of 74 - 630 NTU and 4.1 - 38.5 mg/L, respectively. They increased most in the range from 135.0 - 270.0 mg Al3+/L. The solid-liquid separation condition was quantitatively compared to the correlation of SS removal efficiency between the coagulant dosage and SS concentration based on the concentration of aluminum ions. The empirical formula,   , shows the relationship between SS removal efficiency (R) and coagulant dosage (D) at 38.5 mg/L; it produced high correlation coefficients (r2) of 0.9871 for aluminum sulfate and 0.9751 for PAC.

This study was carried out to investigate the physicochemical and antioxidant properties of fermented milk with addition of hot water extract of Cordyceps militaris grown upon Tenebrio molitor. The general components of Cordyceps militaris grown upon Tenebrio molitor are moisture 10.85%, crude protein 18.44%, crude fat 2.07%, crude ash 5.46%. The DPPH radical scavenging activities of different solvents were the highest (74.81 EDA%) with hot water extract sample. The acidity of fermented milk was high with increasing amount of extracts. The pH of fermented milk reached 4.60-4.66 after 4 h of fermentation, and the number of lactic acid bacteria was highest (11.70 log CFU/mL) with 1% fermented milk. The moisture content of fermented milk showed no significant difference. In addition, contents of crude protein were not significantly different according to addition amount. Regarding Hunter’s color values, L value decreased as the amount of extract increased, whereas a value and b value increased. The content of free amino acid increased with increasing amount of extract. The DPPH free radical scavenging ability and ABTS free radical scavenging ability of fermented milk were significantly different as the addition amount of extract increased. In the sensory evaluation, fermented milk containing 1% of extract showed the highest preference.

환경부에서 발간한 2016 환경백서에 따르면 2016년도 말 현재 주요축종(한육우, 젖소, 돼지, 닭･오리)의 경우 10만 5천여 축산농가에서 19,190만 두의 소, 돼지 등을 사육하고 있는데, 가축사육 두수가 증가됨에 반해 축산농가는 감소하고 있어 전업화, 기업화 추세가 지속되고 있는 중이다. 주요 축종의 가축분뇨 연간 발생량은 46,988천 톤이며, 가축 분뇨의 처리는 발생량 중 약 90.6%는 자원화시설에서 퇴비･액비화 하고 있으며, 8.2%는 개별시설이나 공공처리시설을 통해 정화처리하고 있다. 그러나, 가축사육두수의 증가에 따라 일부 지역에서의 지역 내 수용할 수 있는 환경용량을 넘어서는 양의 가축분뇨가 발생되면 일부 불량한 품질의 가축분뇨가 유통되어 악취 등 생활환경 문제를 야기함과 함께 살포지를 확보하지 못하고 생산된 퇴비･액비가 수요가 없어 지속적으로 쌓이게 되고 저장할 공간 및 시설부족에 따라 퇴비·액비가 강우 등에 노출되어 인근 유역에 유입됨에 따라 수질에 중대한 영향을 미치는 상태가 발생한다. 따라서, 본 연구에서는 가축분뇨를 고압착 필터프레스를 이용하여 고 함수율(80%내외)의 1차 탈수슬러지를 60% 내외의 저 함수율 탈수케이크로 전환하는 고효율 재탈수공정 후 진공감압 가열건조 함으로써 에너지 소비를 절감하고 Dies 형태의 성형장치를 이용한 펠렛 형태의 고체연료를 생산하는 기술을 개발하고 실증화 실험을 수행(고체연료 10ton/일 생산)하였다. 고압착 필터프레스를 이용하여 고액분리 시 가축 분뇨는 유기물 함량이 높아 난탈수성이므로 탈수율을 증가 시킬수 있는 효과적인 탈수 보조제의 종류 및 함량의 변화에 따라 나타나는 차이를 분석 해석을 실시하였다. 또한 건조 시 저온에서도 물의 증발이 원활히 이루어질 수 있도록 진공상태를 부여하고, 고압착 필터프레스로 건조 시작 지점을 점착성 단계를 지나 입자화 단계에서 진행함에 따라 건조 속도 및 건조열효율의 효과를 알아보고자 하였다. 건조 후 Dies 형태의 성형장치를 이용하여 펠렛 형태로 성형된 가축분뇨 고체연료가 가축분뇨 고체연료시설의 설치 등에 관한 고시의 가축분뇨 고체연료 기준에 만족하는지에 대하여 알아보고자 하였다.

본 연구의 목적은 조준과제의 난이도에 따른 정신적 준비기의 뇌 활성화를 비교하는 것이다. CNV파형을 측정하기 위해 남, 여 대학생 17명(남자=13명, 여자=4명)을 대상으로 하였다. 과제는 테이블 위에 고정되어 있는 중심원이 같은 두 개의 과녁(큰과녁=40cm, 작은과녁=10cm)에 스피커를 통해 나오는 대, 소 음성신호가 제시되면 공깃돌을 튕겨 과녁 중심에 정확하게 조준하는 과제이다. CNV파형의 차이를 알아보기 위해 EEG 측정영역은 Fz, Cz, Pz로 설정하여 데이터를 수집하였다. 실험설계는 난이도(2)×영역(3)에 대한 이원분산분석을 실시하였다. 연구결과 난이도에서는 쉬운 과제보다 어려운 과제에서 CNV파형의 진폭이 유의하게 크게 나타났다. 그리고 영역에서는 Cz영역이 Pz영역보다 진폭이 유의하게 큰 것으로 나타났다. 이러한 결과는 과제의 난이도가 높을수록 준비기 동안 과제에 대한 기대와 예측이 반영되고 더 큰 인지적 노력이 요구되는 것이다. 그리고 조준동작 준비과정에서 Cz영역이 가장 많이 동원되는 것으로 나타났다. 따라서 본 연구는 조준과제를 수행할 때 과제난이도에 따른 CNV 진폭의 차이를 밝힘으로써 난이도가 과제수행에 미치는 영향을 일반화 할 수 있는 새로운 자료를 제공했다는 데 의의가 있다.

Transgenic plants that over express virus coat protein genes have attracted particular interest from researchers, by virtue of their tolerance to virus infection. The transgenic watermelon rootstock analyzed in this study was established by introducing CGMMV coat protein (cp) under the control of CaMV 35S promoter and NOS terminator (Park et al., (2005) Plant Cell Rep. 24: 350-6). The primary objective of this study was to determine the copy number and integration site of the transgene element, in order to develop detection techniques required for monitoring of the transgenic watermelon rootstock. The Southern blot analysis indicated that a single copy of CGMMV-cp gene was inserted into the genome of transgenic watermelon rootstock. We also identified the genomic sequences flanking the integration site of the transgene by inverse PCR analysis. In an effort to find a sequence usable as an internal positive control for the screening of the watermelon and watermelon rootstock, we found that the Sat and DIP-1 genes appears as one copy within their genomes and is watermelon rootstock- and watermelon-specific. The information of the integrated site and the internal positive control sequence was used to establish a new event-specific PCR-based detection method. In addition, mRNA and protein expression level of the transgene in the transgenic watermelon rootstock and grafted watermelon were investigated. The expression of both mRNA and protein of CGMMV-CP was not detected in the transgenic watermelon rootstocks and watermelons, suggesting that the movement of transgene products from transgenic rootstock to watermelon does not occur at our detection level.

Genetic variation and structure of 9 subpopulations of Symplocarpus renifolius Schott ex Tzvelev on Mt. Cheonma, in Korea, were determined via starch-gel electrophoresis. The genetic diversity at 10 loci for 8 isozymes (P99=66%, A=2.26, Ho=0.212, He=0.230) was found to be considerably higher than that seen in other long-lived perennial plants. On the whole, the genotype frequencies were in accordance with Hardy-Weinberg expectations. Approximately 5%(θ=0.049) of the total variability was among subpopulations. The high levels of observed genetic diversity in S. renifolius were attributed to a universal outcrossing system and other specific factors like differences in age classes and widely scattered individuals around the main distribution. Heterozygosity was highest at a mid-range of elevation(450m~600m). The lowest heterozygosity at lower elevation was attributed to the possible origin of seeds transported by water from upstream regions during the monsoon season. Spatial structure in a subpopulation evidenced a strong autocorrelation between closer individuals within 3~4m of distance. This was assumed to be attributable to the restricted seed dispersal characteristics of S. renifolius. In accordance with the findings generated in this study, some implications regarding the conservation of S. renifolius at the Mt. Cheonma were also presented.