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        검색결과 849

        341.
        2015.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        목 적: 안경광학과 재학생들의 진로정체감, 전공만족도, 대학생활적응과 자아존중감이 학업성취도에 미 치는 영향을 알아보고자 한다. 방 법: D대학교 안경광학과 재학생 108명을 대상으로 설문조사를 실시하였으며, 학업성취도는 대학 입 학 후 2015학년도 1학기까지의 평균평점을 사용하였다. 수집된 자료는 IBM SPSS Statistics 22를 이용하여 신뢰도분석, 요인분석, 빈도분석, 기술통계량, t-검정과 분산분석, 다중회귀분석을 실시하였다. 결 과: 진로정체감, 전공만족도, 대학생활적응, 자아존중감과 학업성취도 간에는 모두 유의한 정적인 상 관관계를 보였고, 학업성취도와 가장 상관관계가 높은 것은 대학생활적응(r=.320)이었다. 학업성취도에 영향을 미치는 요인은 진로정체감의 요인 중 흥미와 재능(β=.356, p=.012)이었고, 전공만족도는 교과만족을 제외한 모든 변인이 유의미했으며, 대학생활적응 요인 중에서는 학업적응(β=.367, p=.032)만이 유의한 영향을 미쳤고, 자아존중감의 하위요인 중 학업성취도에 영향을 미치는 요인은 자기존중(β=.292, p=.047)이었다. 유의미한 변수들을 동시에 투입하여 학업성취도에 영향을 미치는 요인은 일반적인 전공만족도(β =.465, p=.002)와 전공에 대한 인식만족도(β=-.415, p=.003)가 유의한 영향을 미치는 것으로 나타났다. 결 론: 안경광학과 재학생의 학업성적을 높이기 위해서는 대학생활적응과 전공만족도 증진에 필요한 안 경광학과 특성에 맞는 구체적인 프로그램이 필요할 것으로 사료된다.
        4,600원
        342.
        2015.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The study was conducted with statical analysis of data (828 data in 2010, 752 data in 2012, 648 data in 2014) in order to evaluate laboratory awareness difference of research employees working in different types of universities. Results of the study were as follows: First, university institutes in the order of polytechnic colleges, university, and junior college showed the highest laboratory safety awareness in 'awareness and education of laboratorial safety regulation' and 'awareness in laboratory risk factors'. Second, the difference in safety awareness of universities by year(years that conducted current status survey) was the highest in year 2014, then in 2010, and in 2008. Third, the difference of research employees working for laboratory safety management by year(years that conducted current status) showed that university had the highest laboratory safety awareness in year 2010, but it changed to polytechnic colleges in year 2012 and 2014. Through this study, we could recognize the difference in safety awareness of research employees working in university institutes
        4,000원
        343.
        2015.08 KCI 등재 SCOPUS 구독 인증기관 무료, 개인회원 유료
        We introduce the Lee Sang Gak Telescope (LSGT), a remotely operated, robotic 0.43-meter telescope. The telescope was installed at the Siding Spring Observatory, Australia, in 2014 October, to secure regular and exclusive access to the dark sky and excellent atmospheric conditions in the southern hemisphere from the Seoul National University (SNU) campus. Here, we describe the LSGT system and its performance, present example images from early observations, and discuss a future plan to upgrade the system. The use of the telescope includes (i) long-term monitoring observations of nearby galaxies, active galactic nuclei, and supernovae; (ii) rapid follow-up observations of transients such as gamma-ray bursts and gravitational wave sources; and (iii) observations for educational activities at SNU. Based on observations performed so far, we nd that the telescope is capable of providing images to a depth of R = 21:5 mag (point source detection) at 5- with 15 min total integration time under good observing conditions.
        4,000원
        344.
        2015.08 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The purpose of this study was to investigate the relationships between sports commitment, sportswear brand satisfaction, and brand loyalty of female university students in their 20s. Two hundred surveys were distributed to female university students, and 185 responses were used for the final analysis. The SPSS program was used with a reliability test and factor, frequency, path analysis. Based on the literature review, the following five hypotheses were suggested. H1: Cognitive commitment will have a positive effect on the behavioral commitment of sports commitment factors. H2: The behavioral commitment of female university students will have a positive effect on brand satisfaction. H3: Sportswear brand satisfaction will have a positive effect on continuous brand loyalty. H4: Cognitive commitment will have a positive effect on habitual brand loyalty. H5: Continuous brand loyalty will have a positive effect on habitual brand loyalty. The results of this study showed that first, after the factor analysis, there were two factors of sports commitment (behavioral commitment and cognitive commitment). Second, the path analysis results revealed that there were significant relationships between sports commitment factors (cognitive commitment and behavioral commitment), sportswear brand satisfaction, and brand loyalty factors (continuous brand loyalty and habitual brand loyalty). The results revealed that cognitive commitment had a positive relationship with behavioral commitment, which had a positive relationship with sportswear brand satisfaction. Sportswear brand satisfaction had a positive relationship with continuous brand loyalty, which had a positive relationship with habitual brand loyalty. Cognitive commitment also had a positive relationship with habitual brand loyalty.
        4,500원
        345.
        2015.08 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The purpose of menu labeling is to offer eating environments with improved healthy food selection. Similar to U.S. policies, the Korea government enforces the Special Act on Children’s Food Safety and Nutrition for provision of nutritional labeling in chain restaurants. Considering the importance of dining services for college students and their interests in health, this study examined college students’ perception toward nutrition information provided by university dining services. A survey was conducted for university students. Students' recognition of nutrition information for university dining services was lower than recognition of nutrition information for commercial food services. The most common reason cited by students for considering nutrition information of university dining services was customer rights, followed by health maintenance. Students showed a high tendency to use nutrition information of university dining services. College students’ motivation for knowing nutrition information of university dining services included health and value interests. Students’ knowledge of nutrition had a positive effect on their intention to know nutrition information. The findings of this study offer university administrators and contract food service management companies directions for developing menu labeling for university dining services.
        4,000원
        346.
        2015.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The purpose of this study is to illustrate a general collaborative model of team-teaching in a university general English program. While positive effects of team-teaching and some suggestions for teamteaching practices have been reported previously, there has been less attention paid to an instructional model where native teachers and Korean teachers collaborate with each other for the best classroom experience for students. A qualitative research guided the design. Twenty teachers’ team-teaching classes were observed and video-taped for analysis. The findings showed that the two teachers’ constant negotiations of meanings in any stage of the lessons and in classes with diverse linguistic goals was helpful to the students’ understanding. Also, in the stage of presenting particular language expressions (duringlesson), complimenting each other with native teachers’ intuition and Korean teachers’ analytic skills was suggested as an important instructional move. When activities were introduced, modeling by the two teachers and bridging roles by the Korean teachers were a tremendous contribution and recommended for model collaborations. An instructional model and educational implications have been suggested.
        6,600원
        347.
        2015.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The present study explored a reading-based speaking task for its potential to develop EFL students' fluency. Based on an L1 speaking model by Levelt (1989, 1993), the task was developed in a way that students can maximize their fluency in a scaffolding-rich condition: one in the stage of message generation and the other in linguistic encoding. The use of reading texts for the development of speaking fluency was also hypothesized to induce authentic interaction among interlocutors. How the reading-based speaking task functions as hypothesized was tested with five Korean college students who participated in a 4-week reading-based speaking program. The analysis of the group activity transcripts and interviews revealed that scaffolding in message generation took place in three types of language episode: a personal experience episode, a vocabulary-related episode, and a contentclarification/ elaboration episode. Linguistic encoding in L2 speaking was scaffolded via shared context, which enabled the participants to develop and utilize a paraphrasing skill. Finally, the participants’ engagement in speaking activities increased, affecting their willingness to communicate and their motivation to improve speaking.
        6,600원
        348.
        2015.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study was conducted to provide fundamental data on the awareness of radiation, specifically the differences between general awareness, psychological state and harmful effects. Data was collected from 334 University students in the Chungbuk region through a self-administered questionnaire. According to the results of the reliability and factor analysis, the awareness of radiation was 2.80, with a Cronbach alpha coefficient of 0.815 and KMO of 0.798. Factor analysis extracted three components of the awareness of radiation, which we named general awareness (factor 1), psychological state (factor 2), and harmful effect (factor 3). There were significant differences in the general awareness of natural radiation and radiation-containing foods (p<0.05), whereas no significant differences were found in the general awareness of radiation food supply (p>0.05). Also, the psychological state showed a significant difference in exposure inspection, future exposure, and radiation-containing food (p<0.05), whereas no significant differences were found in the refusal of radiation inspection (p>0.05). The harmful effects showed a significant difference in the rejection of radiation (p<0.05), but no significant differences in the cancer and genetic effects, diseases and physical harmfulness (p>0.05). A significant positive result (p<0.05) was found for the psychological state according to the harmful effects of radiation. Based on this study, detailed and continuous education must be accomplished by increasing the awareness of radiation and the acceptance level, conveying a proper understanding of radiation and assisting subjects with receiving the information they desire through various educational mediums.
        4,000원
        349.
        2015.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The purpose of this study was to evaluate the sodium-related dietary attitudes, behaviors, and nutritional knowledge in university students according to the practice of dietary guidelines for Koreans established by the Ministry of Health and Welfare, Republic of Korea. Based on the total practice score related to the dietary guidelines (PDG), we classified subjects into a “low practice of the dietary guidelines (LPDG)” group (n=94, male=43, female=51) (total score of PDG ≤13) and a “high practice of the dietary guidelines (HPDG)” group (n=56, male=32, female=24) (total score of PDG >13). Subjects were asked about general characteristics, lifestyle, salt-related dietary attitudes, behaviors, and nutritional knowledge using a questionnaire. The LPDG group had more subjects who were breakfast skippers (p<0.001) compared to the HPDG group. The LPDG group (31.26) had a significantly lower score in terms of salt-related dietary attitudes compared to the HPDG group (33.77) (p=0.0042). The score for salt-related dietary attitudes was significantly higher in the HPDG (32.52) group than in the LPDG (29.91) group (p=0.0041). There was no significant difference in the total score for salt-related nutrition knowledge between the groups. The correlation analysis indicated that the dietary guidelines practice score had a positive correlation with the salt-related dietary attitude score (r=0.3593, p<0.0001) and the dietary attitude score (r=0.3443, p<0.0001) after adjustments for sex. These results show that the degree of adherence to the dietary guidelines for adults may be related to sodium-related dietary attitudes, behaviors, and nutrition knowledge.
        4,200원
        350.
        2015.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Recent university students are under a lot of stresses due to academic performance, employment, and anxiety about the future in the fierce competition. Mobile games can be used as a plan which can reduce the stresses of these students. However, if they play too long the games, it will cause another big problems. In this paper, we experimented to look for the best mobile game time to alleviate the stresses of university students as follows. First, we chose 16 people which have more stress load than the average student through stress tests by the basic diagnosis questionnaire. Second, we did total eight experiments on the stresses of the subjects in the study. That is, the experiment was carried out once before the test, 6 times for the mobile game (60 minutes), and once before the experiment. Third, we did T-test and multivariate analysis on the collecting data. As a result, it is proved that the mobile game for about 20 minutes could derive the effect on reducing stresses.
        4,000원
        351.
        2015.06 구독 인증기관 무료, 개인회원 유료
        There are so many animals, mostly dogs, that have been abandoned by their families to roam around the streets of Korean cities and towns. These animals often die in a miserable way such as being run over or being killed and eaten. It is a terrible site to see these dogs, lost and confused in a world without kindness. One solution to the problem is adoption, as it can save life and by example become a driving force to fundamental change in the thinking of other and in society. We are aimed to conduct the survey research to evaluate the university students's awareness of the adoption of the abandoned animals. We deals with the findings obtained from extensive interviews with a representative samples. The objectives of interviews included 789 students in Wonkwang, Keimyung and Chosun University. Questions focused on how students were recognized the need and policies of the adoption of the abandoned animals. Questionnaire and technical data are analysed and summarized. As the results of the survey research, the objectives of interviews had well understood about the policies of adoption of the abandoned animals. Conclusionally, the promotions of adoption of the abandoned animals were need. The effective promotions of the adoption of the abandoned animals will be desirable for the establishment of abandoned animal welfare.
        4,200원
        352.
        2015.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        본 연구는 캠퍼스 내 대학생들의 건강증진 가능성을 알아보기 위해 대학생의 건강상태, 신체활동 수준, 그리고 워커빌 리티에 영향을 주는 캠퍼스 공간에 대한 만족도를 확인해보고자 하였으며, 그 결과는 다음과 같다. 첫째, 대학생들의 건강상태 요인으로서 건강관련 삶의 질 측정결과 5개 영역 모두 건강한 상태였으나, 불안/우울 영역 에서 상대적으로 낮은 수준이었다. 또한, 조사대상자의 과반수가 저체중 또는 과체중・비만으로 건강증진의 필요성이 있음을 보여주었다. 둘째, 학생들은 신체활동 중 걷기/산책을 가장 선호한다고 응답하여 캠퍼스 워커빌리티 향상의 필요성을 제시하는 결과라 볼 수 있지만, 대학생들의 실제 캠퍼스 내 걷기수준은 단지 33.6% 만이 규칙적인 운동을 하고 있으며 대부분의 학생들은 앞으로 규칙적인 신체활동을 할 의사가 있다고 응답하였다. 특히 여학생의 경우 단지 18%(남학생, 48.4%)만이 규칙적으로 운동을 실천하므로 앞으로 여학생의 신체활동을 증가시키기 위한 환경을 조성할 필요성이 있겠다. 이와 더불어 신체활동의 건강증진 효과를 기대하기 위해서는 신체활동 시간도 중요하기 때문에 체육시설뿐만 아니라 캠퍼스의 보도, 산책로 등의 환경개선을 통해 1시간 이상 걷는 학생들의 비율을 증대할 필요성이 있다고 사료된다. 마지막으로, 계명대학교 학생들의 캠퍼스 공간에 대한 만족도를 조사한 결과 100점 만점으로 환산했을 때 54.8점에서 68.0점 수준으로 조사되었다. 걷기와 관련한 심리적 측면과 산책로에 대해서는 만족하고 있었으나, 걷기와 관련한 심미적 측면과 교통사고 안전 및 범죄 안전성 그리고 여성 혼자 걷기나 야간에 걷기의 안정성에 대해서는 다소 만족하지 않는 것을 알 수 있었다. 캠퍼스의 골격을 이루는 차량중심의 도로체계는 보행성을 저해하는 가장 큰 요소라 판단되며, 장기적 관점에서 보행자가 우선이 되는 캠퍼스 환경 개선이 요구된다 하겠다.
        4,000원
        353.
        2015.05 KCI 등재 구독 인증기관 무료, 개인회원 유료
        본 연구는 산학협력단의 리더십역량, 전략역량 등 핵심역량이 기술이전과 교육 성과에 미치는 영향을 분석한 연구이다. 우선, 산학협력단과 관련한 문헌연구를 실시하였고, 전국 산학협력단을 대상으로 설문조사를 실시하였다. 본 연구에서는 산학협력단 주요 경영 성과인 기술이전성과와 교육성과를 종속변수로 설정하였다. 독립변수로는 리더십역량, 전략 역량, 인적자원역량, 연구개발 역량을 설정하고 각각에 대한 조작적 정의를 부여 하였다. 변 수간 인과관계분석을 위한 연구모형을 제시하였으며, 설문대상 간의 차이 분석을 위해 국공 립 대학과 사립대학을 조작변수로 연구모형에 포함하였다. 실증분석 결과, 독립변수로 적용 한 리더십역량은 기술이전성과와 교육성과에 영향을 미치지 않는 것으로 조사되었으며, 마찬 가지로 전략역량은 교육성과에만 영향을 미치는 것으로 검증되었다. 인적자원역량의 경우에 도 종속변수에 미치는 영향이 없었으며, 연구개발 역량의 경우에는 기술이전성과에 영향을 미치는 것으로 분석되었다. 본 연구의 결과가 산학협력단의 경영성과 개선에 도움이 되기를 기대한다.
        6,400원
        354.
        2015.05 KCI 등재 구독 인증기관 무료, 개인회원 유료
        홍콩과 마카오 지역에서 시행되는 유아교육과 초중등교육에서 시행되는 모든 교육은 번체자를 사용하고 있으며, 이 지역에서 출판되는 신문과 잡지 또한 대부 분 번체자를 사용하고 있다. 그러나 홍콩, 마카오 지역과 중국 대륙 사이의 교류가 점점 활발해 짐에 따라 민간에서 사용되는 간화자의 비율은 점점 높아지고 있으 며, 여러 곳에서 번체자와 간화자가 함께 사용되는 현상이 늘어나고 있다. 또한홍 콩고시평가원에서는 공개적으로 공식적인 시험에서 표준화된 간화자를 사용할 수 있다고 명확히 표명하여, 홍콩의 중학생들의 학습과 한자 쓰기에 대한 심리적 태 도에 일정한 영향을 미쳤다. 홍콩대학생의 간화자 사용에 대한 정확한 상황을 조 사하기 위해서 우리는 설문지를 세 부분으로 나누어 조사하여 보았다. 우선 2백여 개의 상용 간화자를 이용하여 학생들의 간화자 분별 능력을 조사하였고, 다시 5백 여개의 간화자를 선정하고 이를 이용하여 어휘를 작성하여 학생들의 간화자 분별능력을 조사하였다. 세 번째로 백 여개의 간화를 이용하여 학생들이 간화자를 올 바르게 쓰는가를 측정하였다. 조사 결과는 홍콩과 마카오의 대학생들의 간화자에대한 분별 능력과 쓰기 능 력을 보여주었는데, 홍콩과 마카오 대학생 대부분이 중국 대륙의 간화자를 분별하 였으나 한자를 쓰는데 있어서는 종종 홍콩식 간화자, 곧 대륙의 간화자형이 아닌 간화자를 사용하기도 하였다. 이는 향후 홍콩과 마카오의 어문 교육에 있어서 참 고할 만한 사실로, 한자쓰기 교육을 강화하여 학생들에게 홍콩식 간화자를 사용치 않도록 주의를 기울여야 함을 말해 주고 있다.
        5,800원
        356.
        2015.04 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study was conducted using a self boarding questionnaire survey to investigate body image perception, dietary habits and nutrient intakes according to interest level in health of female university students in Masan area (n=302). The subjects were divided into 2 groups according to interest level in health (‘High’ group, n=101, ‘Low’ group, n=201). Body image according to BMI was significantly different regardless of interest level in health (p<0.001). The answer percentage indicated that the purpose of weight control is health, regular use of the scale, and regular exercise were significantly higher in the high group (p<0.05). Nutrient knowledge score (p<0.01) and food frequency score (p<0.001) were significantly higher in the high group (p<0.01). There were no significant differences in nutrient intake, but intake, NAR and INQ of vitamin C were significantly higher in the high group (p<0.001). Nutrition knowledge score (p<0.01), food intake frequency score (p<0.01), and INQ and NAR of vitamin C (p<0.01) were positively correlated with the interest level in health. These results will be useful as a basis for the development of effective nutrition education programs in order to increase interest level in health and apply well in real life what have learned through the correct nutrition knowledge.
        4,600원
        357.
        2015.04 KCI 등재 구독 인증기관 무료, 개인회원 유료
        본 연구는 노원구 S대에 재학 중인 남자 대학생을 대상으 로 lacto-ovo-vegetarian과 non-vegetarian으로 나누어 신체계측, 건강상태, 식습관 및 영양소 섭취상태를 비교하여 건강에 대 한 기초 자료로 사용하고자 실시하였다. 1. Lacto-ovo vegetarian과 non-vegetarian의 신장은 차이가 없 었지만, 체중은 non-vegetarian에서 유의하게 높았다(p<0.05). 2. Lacto-ovo vegetarian은 종교적인 이유로 채식을 하고 있 었으며, 영양학적으로 채식 식사의 만족도는 86.0%를 차지하 였다. 3. Lacto-ovo vegetarian과 non-vegetarian은 건강에 대한 관 심도와 영양제 복용율은 낮은 편이었으며, 운동을 하는 비율 은 lacto-ovo vegetarian 64.0%와 non-vegetarian 61.4%가 한다고 답하였다. 4. Lacto-ovo vegetarian은 non-vegetarian에 비해 식사를 규 칙적으로 하였으며, 매일 아침식사를 하는 비율이 높았다. 또 한 전체 조사대상자들은 모두 외식을 한다고 답하였으며, 외 식 시 선호하는 음식으로는 한식을 선호하는 것으로 조사되 었다. 5. Lacto-ovo vegetarian은 non-vegetarian에 비해 우유나 유 제품(요구르트, 요플레 등)을 매일 1병 이상 마시는 점, 김치 이외의 채소를 식사할 때마다 먹는 점, 과일(1개)이나 과일주 스(1잔)를 매일 먹는 점, 식사는 매일 세끼를 규칙적으로 한 다는 점 및 모든 식품을 골고루 섭취하는 점에 대해 높은 경 향을 보였다. 이에 비해 non-vegetarian은 달걀, 콩 및 두부 등 으로 된 음식을 끼니마다 먹는 점과 튀김이나 기름에 볶는 요리를 주 2회 이상 먹는 점이 높은 경향을 보였다. 6. Lacto-ovo vegetarian의 경우, 에너지, 비타민 B2, B6 및 니아신 섭취율이 낮은 반면에, 식이섬유는 충분히 섭취하고 있었다. 그러나 non-vegetarian은 식이섬유는 부족하게 섭취 하였으나, 소디움, 콜레스테롤 등의 영양소는 과잉 섭취하는 것으로 조사되었다. 따라서 본 연구 결과를 종합해 보면, 건강관련사항이나 식 습관에 있어서는 두 군 간에 큰 차이는 없었지만, 영양소 섭 취상태에서는 lacto-ovo vegetarian과 non-vegetarian에 있어서 유의한 차이를 보였다. Lacto-ovo vegetarian의 경우에는 육류 나 생선 섭취를 하지 않는 것을 고려하여 열량을 비롯한 다른 영양소의 섭취를 충분히 할 수 있도록 신중한 식사계획이 필 요하며, 식품교환표를 이용한 대체식품의 이용률을 증가시켜 야 할 것으로 사료된다. 또한 non-vegetarian의 경우는 지속적 인 영양소의 과잉 섭취로 인한 건강상의 문제점이 발생되지 않도록 노력해야 할 것이다.
        4,300원
        358.
        2015.04 구독 인증기관·개인회원 무료
        Species diversity of ant fauna inhabiting the university campus in the Seoul Metropolitan city, Hanyang University is documented in this investigation. Totally, 14 species of 8 genera belong to 3 subfamilies discovered in study area. Our results are compared with the previous works on ant fauna in the forest and urban habitats based on species number difference. In study area, site 12 and 13 contain wide forest area and diverse environmental elements such as rotten wood, leaf mold and group of living tree. Site 7 has small forest area, but show wide variety of environments like site 12 and 13. Urbanized region like site 8, 10 or area in construction like site 1 show the most lowest diversity. Aside from diversity, Four ant species, Paratrechina flavipes, Lasius spp., Camponotus japonicas, Tetramorium caespitum, appear at almost all habitat, seems to have strong tolerance to environmental change.
        359.
        2015.04 구독 인증기관·개인회원 무료
        □ 특성화 사업단의 교육비전과 목표 ○ 사업단 교육비전은 “국가 지역사회의 안전 녹색경제 발전에 기여“에 있음 ○ 교육목표는 “기후변화관련 기본 응용 교육과 현장훈 련을 통한 관련분야 융합 지식을 갖춘 전문 인재 양성“임 ○ 주요 추진전략은 “교육시스템의 혁신”에 있음 □ 특성화 사업단 지원 및 육성 전략 ○ 3개의 사업부(기획부/행정부, 사업부, 국제/산학협력 부)와 사업단 운영·자문을 지원하는 2개의 위원회(운영위원 회, 외부 자문ㆍ평가 위원회)로 구성
        360.
        2015.03 구독 인증기관 무료, 개인회원 유료
        Kidney cells of canine embryos were separated into single cells using collagenase and dispase. Primary culture was conducted using these cells. To remove fibroblasts, these cells were treated with edetate disodium dihydrate (Na2EDDA), and pure epithelial cells were separated. Recombinant retrovirus particles that manifest teromerase were produced and inoculated into primary culture cells to produce immortalized canine cell strains (JNUCK-1 and JNUCK-2). To examine the characteristics of the produced cell strains, the growth curve, maximum cultured households, and expressed proteins (keratin) were identified. The JNUCK-1 and JNUCK-2 cell lines showed division ability until the 30th generation without growth retardation. JNUCK-1 and JNUCK-2 cell lines clearly expressed telomerase until the 25th generation. The canine distemper virus (CDV) was inoculated into the JNUCK-1 and JNUCK-2 cell lines, as well as in the Madin–Darby canine kidney (MDCK) cell line. The maximum titer of CDV from the JNUCK-1 cell strain was about 200 times higher than that from the MDCK cell strain. However, the JNUCK-2 cell strain produced a lower titer than the MDCK cell strain. We established a new canine kidney epithelial cell line (JNUCK-1) that could produce CDV with high titer.
        4,000원