본 실험은 작물의 양』분 흡수율에 근거하여 배추과 엽채류 수경재배에 적합한 배양액을 개발하고자 수행되었다. 원예연구소 배양액(NSH액) 1배액을 기준으로 1/2배액, 1배액, 3/2배액으로 25일간 담액 수경재배 했을 때 청경채와 겨자채는 3/2배액, 케일은 1배액에서 생육이 좋았다. 작물의 양수분 흡수율을 산정하여 얻은 평균 양이온 비는 K 49.5%, Ca 35.8%, Mg 14.7% 였으며, 배추과 작물에 적합한 배양액 N 14, P 3, K 6.8, Ca 4.8, Mg 2m·L-1을 개발하였다 개발 배양액을 평가하기 위해 2003년 9월부터 2004년 10월까지 공시 작물을 4회 재배하였다. 개발 배양액에서 작물을 재배했을 때 빠른 상대생장률과 함께 생육이 1.1~2.5배, 비타민 C 함량은 1.06~l.52배 증가하였다. 개발 배양액에 적합한 작물로는 겨자채 'Asia curled', 'Asia redcurled' 및 'Pamagreen', 케일 'TBC', 'Portugal' 및 'Manchu collard', 잎브로콜리 'New green', 천경채, 방울다다기양배추 'Green king'와 'Red king', 홍채태, 쌈추 등으로 생육과 항산화 성분인 비타민 C함량 증가를 통해 기능성 엽채류 생산이 가능하였다.

다양한 종류의 생약재들이 동물과 인간의 성장률을 증진시키는 활성을 가지고 있는 것으로 보고되고 있다. 선정된 식물의 성장증진 효과를 확인하기 위해 실험동물로서 물고기와 돼지를 이용하였다. 물고기 실험에서 오가피 추출물과인진쑥 추출물이 가장 효과적인 것으로 나타났다 그리고 이들 오가피와 인진쑥 추출물을 돼지에 먹여본 결과 평균일일 증체율이 대조군에 비해 7.06%증가하였다. 이 결과를 검증하기위해 혈액 내 분비된 성장호르몬(GH)과 Insulin-like growth factor-1(IGF-1)의 양을 측정하였다. 오가피와 인진쑥 추출물을 먹인 돼지는 대조군에 비해 혈액 내로 분비된 GH와 IGF-1의 양이 훨씬 더 많았다. 인간에서 성장증진 효과를 알아보기 위해 본 연구자들은 오가피와 인진쑥 추출물을 혼합하여 쌀에 코팅한 키우미^(TM)(Kiwoomi^(TM))를 제조하였다. 키우미^(TM)를 초등학생들에게 먹였을 때 키 성장에 효과적인 것을 확인하였다. 키우미^(TH)를 먹인 아이들이 먹이지 않은 아이들보다 더 많이 성장하였다(약 2.14배). 결과적으로 이 기능성 쌀(Kiwoomi^(TM))이 다른 부작용 없이 아이들의 성장에 도움을 주는 것으로 판단된다.

참게(E. sinensis)의 유생 생장에 미치는 기초적인 환경 및 생물학적 정보를 얻기 위해 본 실험을 실시하였다. 참게 유생을 카드뮴과 수은의 0.1, 0.2, 0.3 ppm농도에 처리하였다. 참게의 생존율과 카드뮴과 수은의 중금속 농도와 양의 상관을 나타내었다. 그런데 96시간 사육시 카드뮴과 수은의 반수치사농도는 유생 단계간 유의한 차이를 나타내었다. 반수치사농도는 카드뮴이 수은보다 높았으므로 수은이 더 치명적이였다. 중금속의 축적은 카드뮴과 수

복제수정란 생산에 있어서 수핵란 내 체세포 주입 후 전기적인 융합은 필수과정인데, 이 과정을 거치는 동안 많은 수의 체세포 주입 난자가 융합에 실패하거나 lysis가 일어나게 된다. 본 실험에서는 한우 체세포를 이용하여 핵이식을 실시한 후 수핵세포질과 응합을 시도할 때 전기융합 방법에 따른 융합율과 배발달율을 검토하고자 실시하였다. 공여세포는 한우 귀 세포조직을 채취하여 0.05% trypsin과 EDTA가 첨가된 D-PBS로 세포를 분리한 후 DMEM

This paper is to develop replacement models under minimal repair with exponential polynomial wavelet failure rate function. Wavelets have good time-frequency localization, fast algorithms and parsimonious representation. Also this study is presented along with numerical examples using sensitivity analysis for exponential polynomial trigonometric failure rate function.

The evaluation of mental workload is measured by subjective ratings, physiological signals. It takes long time to analysis the measured signals and is very tedious and time-consumming work. Therefore, to evaluate the affect of workload effectively, real-time measurement system is required. In this paper, real-time mental workload measurement system using cardiac autonomic indiced which reflect well the mental workload was developed and evaluated. Analyzed indices were HR, IBI, Lorentz plot, CSI, CVI, and LF/HF ratio of heart rate variability. The system was applied to evaluate the affect of arithmetic task and showed good results. This system was consisted of ECG amplifier, A/D converter, and personal computer, and algorithm was implemented using LabVIEW.

This study was conducted to investigate the effects of vitrification solution and developmental stage on the survival rate of vitrified-thawed human blastocyst embryos. Human blastocyst embryos were cryopreserved by vitrification using EFS and GE solution, and their survival rates were examined after thawing and further culture. EFS solution was consisted of 40% ethylene glycol, 18% Ficoll 70 and 0.3M sucrose. GE solution was consisted of 25% glycerol and 25% ethylene glycol. Embryos were exposed to EFS and GE solution by 2 steps and 3 steps, respectively, and plunged into liquid nitrogen after loading into 0.25ml plastic straws. Blastocysts were classified into 4 groups in accordance with their developmental stage: into 1) EEB, 2) MEB and 3) EdB, of blastocysts developed on day 5, and 4) 6d-Bla(the blastocysts which formed on day 6). The blastocysts at each stage were vitrified by GE solution and cryopreserved in LN2. After thawing them, we examined their survival rates, respectively. The resulted of this study were as follows: 1. The survival rate of blastocysts vitrified by GE solution was 64.4%, significantly higher than that (5.7%) vitrified by EFS solution (P<0.001). 2. When the blastocysts were vitrified by GE solution according to each developmental stage, the survival rates of EEB, MEB, EdB and 6d-Bla were 65.9%, 65.9%, 73.2% and 58.1%, respectively. In conclusion, the cryopreservation of human blastocysts by vitrification is likely to have a marked advantage in terms of cost, work and time as compared to the conventional slow freezing in IVF-ET programs.

The objective of this study was to evaluate the embryonic development ability and the appearance of blastocysts of bovine in vitro fertilized oocytes cultured in different culture media, and also to evaluate survival rate after thawing of frozen embryos by using 1.5 or 1.8M ethylene glycol(EG) with sucrose or trehalose. Fertilized oocytes were divided into three groups; i ) monolayer of cumulus /granulosa cell prepared by TGM 199+5% calf serum(TGM199), ii)GRlaa+5% CS, iii)SOF+5% CS, and they were cultured after insemination for 9 days, at 39˚C, under 5% in air, but SOF+5% CS was cultured at 39˚C, under 5% 02, 5% GO2, 99% N2. Blastocysts derived from GRlaa + 5% CS on day 7~8 after insemination were frozen by using 1.5M EG or 1.8M EG with/without 0.2M sucrose or O.1M trehalose. The development rate of blastocysts on day 7 after insemination in SOF+5% CS was significant higher than in TCM199 or CR1aa(P<0.05). The appearance rate of blastocysts on day 7-8 after insemination was higher than in TCM199, when fertilized oocytes were cultured in GRlas or SOF. The survival rate of frozen blastocysts after thawing tended to increase, when blastocysts were frozen by using 1.8M EG with 0.2M sucrose or O.1M trehalose. These results indicated that SOF or CRlaa media with amino acids was superior to TCM199 with monolayer in terms of blastocyst development in culturing of in vitro fertilized bovine nocytes, and sucrose or trehalose was supposed to prevent embryos from the freezing shock.

These experiments were carried out to determine the effect of pregnancy in bisected embryo. The embryos of ICR mouse were microsurgically bisected at morula and blastocyst stage using microsurgical blade attached a micromanipulator. These bisected embryos without zona pellucida were cultured up to blastocyst stage and cell count and diameter of stained blastomere, and transferred pseudopregnant mice. And the development of these bisected embryos was compared with the results of production of young of the corresponding intact embryos or cell stage. When the bisected mouse embryos were cultured in vitro for 20 to 24 hours in morula stage(77.2%) or 3 to 6 hours in blastocyst stage(84.1%), them were developed to the expanded blastocyst stage. There were no significant(P<0.05) differences in the development rate of bisected embryos between in morula and blastocyst stages. The embryo size of blastocyst developed in vitro from bisected embryo was small(P<0.05)than intact embryo. However, the number of blastomeres with bisected embryo (24.7+1.3and 21.5+1.2 respectively) were significantly(P<0.05) reduced, compared with that of intacted embryos(36.3+1.1 and 41.4+1.2 respectively). When compared with the result of pregnancy rate(63.6%) after surgical transfer of bisected morulae, a similar result(65.4%) was obtained with bisected blastocyst stage(P< 0.05). However, production of youngs (38.8%) after transfer of bisected morula, a similar result (38.1%) was obtained with bisected blastocyst stage (P<0.05).

山地草地開發파 聯關된 草地士爆改良 및 施R巴法 改善올 薦하여 Homes의 Systematic variations 方法으로 多量要素 ΣAnion : ΣCation 適正施服比率 및 適正總養分(ΣA+ ΣC) 施肥量을 決定코자 Grass-clover 混播我培條件에서 山地土壞을 供試한 Pot 試驗으로 遂行한 結果는 1. 混播條件에서 各構成草種別 收量提高를 위한 ΣA/Σ

This experiment was carried out to investigate the effect of shading degrees (0:full sunlight, 25, 50 and 75%) on the crop growth rate (CGR), net assimilation rate (NAR) and photosynthetic activity of grasses for obtaining the basic data on the developmen

본 연구의 목적은 기상자료(강수량, 최고기온, 최저기온, 평균기온, 평균풍속) 기반의 다중선형 회귀모형을 개발하여 농업용저수지 저수율을 예측 하는 것이다. 나이브 베이즈 분류를 활용하여 전국 1,559개의 저수지를 지리형태학적 제원(유효저수량, 수혜면적, 유역면적, 위도, 경도 및 한발빈도)을 기준으로 30개 군집으로 분류하였다. 각 군집별로, 기상청 기상자료와 한국농어촌공사 저수지 저수율의 13년(2002~2014) 자료를 활용하여 월별 회귀모형을 유도하였다. 저수율의 회귀모형은 결정계수(R2)가 0.76, Nash-Sutcliffe efficiency (NSE)가 0.73, 평균제곱근오차가 8.33%로 나타났다. 회귀모형은 2년(2015~2016) 기간의 기상청 3개월 기상전망자료인 GloSea5 (GS5)를 사용하여 평가되었다. 현재저수율과 평년저수율에 의해 산정되는 저수지 가뭄지수(Reservoir Drought Index, RDI)에 의한 ROC (Receiver Operating Characteristics) 분석의 적중률은 관측값을 이용한 회귀식에서 0.80과 GS5를 이용한 회귀식에서 0.73으로 나타났다. 본 연구의 결과를 이용해 미래 저수율을 전망하여 안정적인 미래 농업용수 공급에 대한 의사결정 자료로 사용할 수 있을 것이다.

The Accurate Ranging System for Geodetic Observation – Mobile (ARGO-M) was successfully developed as the first Korean mobile Satellite Laser Ranging (SLR) system in 2012, and has joined in the International Laser Ranging Service (ILRS) tracking network, DAEdeoK (DAEK) station. The DAEK SLR station was approved as a validated station in April 2014, through the ILRS station “data validation” process. The ARGO-M system is designed to enable 2 kHz laser ranging with millimeter-level precision for geodetic, remote sensing, navigation, and experimental satellites equipped with Laser Retro-reflector Arrays (LRAs). In this paper, we present the design and development of a next generation high-repetition-rate SLR system for ARGO-M. The laser ranging rate up to 10 kHz is becoming an important issue in the SLR community to improve ranging precision. To implement high-repetition-rate SLR system, the High-repetition-rate SLR operation system (HSLR-10) was designed and developed using ARGO-M Range Gate Generator (A-RGG), so as to enable laser ranging from 50 Hz to 10 kHz. HSLR-10 includes both hardware controlling software and data post-processing software. This paper shows the design and development of key technologies of high-repetition-rate SLR system. The developed system was tested successfully at DAEK station and then moved to Sejong station, a new Korean SLR station, on July 1, 2015. HSLR-10 will begin normal operations at Sejong station in the near future.

This study was carried out in order to investigate the early growth and root development characteristics of Peucedanum japonicum container seedlings. The experiment was performed by containers (128 and 200 cavities containers) and shading levels (0%, 35%, 50% and 75% shading). Germination rate of P. japonicum seeds was getting higher in the lower shading level and the highest in the full sunlight (71.9%). Plant height was the highest under 50% shading of all containers. Fresh weight and dry weight of the shoot (leaves + stem) were the highest under 50% shading of all containers, too. Meanwhile, fresh weight and dry weight of the root per plant were the highest under the full sunlight of 128 cavities container such as 0.34 g and 0.03 g, respectively. Total root length, root project area and root surface area were higher under the full sunlight of 128 cavities container such as 234.5 cm, 6.6cm2 and 20.8cm2, respectively and the next higher was under 35% shading such as 201.7 cm, 5.9cm2 and 18.4cm2, respectively. A case of root volume was the highest with 0.15cm3 under the full sunlight. As a result of the surveying the whole experiment, it is concluded that the shoot and root of P. japonicum seedling grow nicely by maintaining 35% shading.

본 연구에서는 지하수 양수에 따른 하천수 감소량을 예측할 수 있는 상관관계식을 지표수-지하수 통합모의 결과를 이용하여 유도하였다. 신둔천과 죽산천 두 개의 시험유역에 대해 지표수-지하수 통합모형 SWAT-MODFLOW을 적용하여 다양한 양수조건에 따른 하천수 감소량 자료를 모의 생성하였으며, 생성된 자료를 바탕으로 다중회귀분석을 실시하여 지하수 양수량, 하천과 양수정 이격거리, 대수층 및 하천바닥의 수리특성, 강수량 등의 함수인 하천수 감소량 산정 식을 유도, 제시하였다. 개발된 상관관계식은 하천인근 지하수 양수에 따른 하천수 영향을 평가하는데 간편하게 적용될 수 있으며, 지하수 양수량 중 하천수에 해당하는 부분인 하천수 기여도를 산정하는데 활용될 수 있을 것으로 기대된다.