우리나라에서 재배되고 있는 백합 구근의 대부분을 수입에 의존하고 있어 국내 품종을 개발하여 보급할 필요가 있다. 분화용 나팔나리 유전자원 ‘Ace’, ‘Nelli White’, ‘Mount Carmel’을 수집하여 1996년부터 2008년까지 자가수정을 실시하였다. 2014년에 자가수정 7세대 ‘ L2-127-1’과 ‘ L2-81-5’계통을 교잡하여 1대잡종 나팔나리 ‘White Eve’를 개발하였다. 분화용 나팔나리 일대잡종 ‘White Eve’는 백색의 나팔모양의 홑꽃으로 반점이 없으며, 식물체 당 개화수가 5.8개로 많은 편이며, 꽃의 직경이 13.1cm로 중간 정도이고 측향으로 개화한다. 초장은 40.9cm이고, 잎의 수는 32.8개이고, 잎의 길이는 13.5cm이며, 잎의 폭은 2.1 cm이다. 일대 잡종 ‘White Eve’는 대조품종인 ‘Mount Carmel’ 보다 화경이 크고 화수가 많아서 개화 기간이 길며 소비자 선호도가 높다.
국내 최대 규모의 마르형 분화구 내에 형성된 하논은 제주도에서 유일하게 벼농사가 약 500년 동안 유지되고 있는 논 생태계이다. 이러한 하논의 식물상 특성과 복원 방안을 파악하고자 2015년부터 2018년 사이 총 8회에 걸쳐 식물상을 조사하였다. 그 결과, 관속식물은 55과 151속 194종 1아종 26변종 4품종으로 총 225분류군으로 조사되었다. 과별 분포현황은 벼과가 가장 많은 36분류군을 차지하였고, 다음으로 국화과 29분류군, 사초과 20분류군, 콩과 13분류군, 마디풀과 11분류군으로 나타났다. 하논에 출현한 식물의 생활형 조성은 일년생식물-단립식물-중력산포형-총생형 (Th-R5-D4-t)으로 나타났다. 희귀식물로는 물질경이 (Ottelia alismoides (L.) Pers.), 창포 (Acorus calamus L.), 물잔디 (Pseudoraphis ukishiba Ohwi) 3종이 확인되었다. 멸종위기야생식물은 멸종위기야생생물 II급인 삼백초 (Saururus chinensis (Lour.) Baill.) 1종이 확인되었다. 귀화식물은 15과 34속 41종 2변종으로 총 43분류군이 확인되었고, 도시화지수는 13.3%, 귀화율은 16.9%를 나타냈다. 현재 제주도 하논은 멸종위기야생식물, 희귀식물을 포함하여 높은 식물 다양성을 가지고 있을 뿐만 아니라, 제주도 유일의 벼농사 지역으로서 지속적으로 보전해야할 충분한 경관생태적, 사회문화적 가치를 지니고 있다고 판단된다.
본 논문은 디지털 게임의 하위 장르 분화 양상을 고찰하고 각 양상에 따라 핵심 장르 요소가 변화 하는 특성을 규명하는 데에 목적을 둔다. 디지털 게임의 장르는 변화를 거듭하는 생성적 체계이다. 기존 장르의 핵심 메커니즘을 수정하거나 뒤집으며 등장하는 게임의 하위 장르들은 게임의 장르 체계가 지닌 생성적 성격을 확인할수 있는 대표적 사례이다. 하위 장르 분화를 고찰한 기존의 연구들 이 게임 텍스트의 계보학을 통해 메커니즘의 변화를 추적했다면, 본 논문은 플레이어가 장르 변화를 인지하는 원리에 초점을 두고 해당 양상을 분석한다. 개념적 혼성 이론을 통해 ‘로그라이크’와 ‘메트로배니아’ 장르의 분화 과정을 분석한 결과 로그라이크는 상위 장르와의 프레임 대립을 통해, 메트로배니아는 상위 장르의 프레임 확장을 통해 변별력을 확보한 장르임을 확인했다. 프레임 대립에 따라 분화한 하위 장르의 경우 핵심 장르 요소는 독립성을 확보하고 손쉽게 타 장르와 결합되며, 프레임 확장에 따라 분화한 하위 장르의 경우 핵심 장르 요소는 맥락 의존성을 강하게 드러내며 해당 장르의 맥락을 벗어나 사용되기 힘들다는 특징을 보인다.
Undifferentiated pleomorphic sarcoma (UPS) in the mandible region is a rare malignant neoplasm. We encountered the case of a patient who had been diagnosed with deep benign fibrous histiocytoma (DBFH) prior to surgery, but the final biopsy revealed the presence of tumor tissues in the resected margin, which indicated a UPS. Therefore, radiotherapy was conducted post-operatively, but the tumor recurred approximately 3 years later and a second surgery was performed. The difference between the initial and final diagnoses was considered to be due to the characteristics of the tumor, as it involved both benign and malignant components. DBFH and UPS can occur in young women. DBFH can transform to a malignancy and UPS may also appear as a benign tumor during early biopsy. Hence, attention should be paid to diagnosis and treatment methods, and ongoing close observation is required.
Nicotine of tobacco component has a controversial impact in the clinical outcome of dental implants. Although numerous nicotine effects on bone healing around implants have been presented, it is rarely reported in vitro study about normal human osteoblast(NHost) from oral and maxillofacial area at the surface of implants. The purpose of the present study was to evaluate the effect of nicotine on the proliferation and differentiation response of NHost to plasmatic and salivary levels of nicotine reported in smokers at the surface of screw-type plasma-sprayed titanium implants. NHosts were seeded on the surface of titanium implants and cultured for 21 days in α-MEM supplemented with 10% FBS, 50mg/ml ascorbic acid, 5mM β-glycerophosphate and 100nM dexamethasone. Seeded implants were exposed to various nicotine concentration(0.05-0.5mg/ml) from 1 to 21 days, and characterized for cell morphology, proliferation, differentiation, alkaline phosphatase(ALP) activity and ionized calcium concentration(Cai) of medium. Continuous exposure to higher nicotine concentration(above 0.3mg/ml) induced a dose- and time-dependent vacuolation of the cytoplasm, and a tendency to detach from the implant surface. 0.05mg/ml(lower nicotine concentration) did not cause significant effects in the cell proliferation and ALP activity. 0.1-0.2mg/ml caused evident dose-dependent effects in increased cell proliferation, ALP activity and earlier onset of matrix mineralization at levels up to 0.2mg/ml, while a dose-dependent inhibitory effect at 0.3-0.5mg/ml. Cai concentration of control group was decreased at 14 days. Increased Cai concentration at 0.1-0.2mg/ml, decreased Cai concentration at 0.3mg/ml and no change at 0.5mg/ml during the culture period were seen. It suggested that nicotine concentration could paly an role in modulating NHost activity as a contributing factor associated with proliferation and differentiation of NHost at the surface of implants.
본 연구의 목적은 온도와 토양수분에 따른 마늘의 생육, 생리장해 및 수량에 미치는 영향을 구명하고자 실시 하였다. 실험은 온도가 6℃ 차이가 나는 온실에서 수행 되었는데, 정식부터 수확까지 외기(A)보다 3℃(A+3℃), 6℃(A+6℃) 고온조건, 토양수분은 적습(OI) 대비 수확기 무렵의 다습(EI) 처리를 하였다. 그 결과, 마늘 생육특성은 온도와 토양수분 조건(0.34m3/m3)에 따라 고도의 유의성을 보여 고온일수록 그리고 적습 처리구일수록 컸다. 초장은 외기보다 A+6℃-OI 처리구에서 47.4cm로 가장 컸으며, 엽장과 엽폭 역시 외기보다 A+6℃-OI 처리구가 각각 16.1cm, 2.4cm로 가장 컸다. 마늘재배 기간 중 외기보다 3℃, 6℃ 고온조건이 되면 스펀지마늘 발생율이 높아져 A+6℃-OI 처리구는 12.9% 발생하였고, A-EI 처리구에서는 전혀 발생하지 않았으며, 인편무게와 1쪽당 무게는 A+6℃ 고온구에서 크게 감소하여 수량이 외기 대비 A+6℃ 처리구는 평균 51%, A+3℃ 처리구는 평균 22% 감소하였다. 따라서, 마늘재배시 외기보다 6℃ 고온조건과 다습조건이 되면 상품수량 감소하고, 생리장해 발생이 많아지는 것으로 나타났다.
백두산 분화의 전조현상 및 일본 화산의 지속적인 활동으로 인하여 화산재 확산으로 인하여 발생할 수 있는 재난에 대한 위협이 지속적으로 제기되고 있다. 이에 대한 대응을 위하여 국내에서도 화산재해대응시스템을 개발하여 활용하고 있는 실정이며 이에 대응하기 위한 연구의 일환으로 화산재 확산에 대한 확률적 공간 분포 분석이 연구된 바가 있다. 본 논문의 part1이라 할 수 있는 ‘백두산과 아소산 화산재 대기 확산의 확률적 공간 분포 분석’에서는 백두산과 아소산 두 개의 화산에 대하여 FALL3D 수치해석을 수행 하고 그 결과를 이용하여 대기 중 화산재 농도 및 퇴적 두께에 대한 분석을 수행한 바 있다. 본 논문에서는 백두산과 아소산 외에도 국내에 영향을 미칠 수 있는 총 5개의 화산에 대하여 해석기간 및 분석 범위를 확장하여 추가 연구를 진행하였다.
본 연구에서는 전문대학생들의 자아존중감, 자아분화수준, 스마트폰 중독의 관계를 파악하고자 하였다. 전문대학생들의 자아존중감, 자아분화수준이 스마트폰 중독에 미치는 영향을 밝히는데 목적을 두었다. 수집된 285부의 자료는 SPSS WIN 22.0 프로그램을 사용하여 분석하였다. 첫째, 전문대학생의 자아존중감, 자아분화수준, 스마트폰 중독의 일반적 특성에 대해 살펴보았다. 둘째, 전문대학생의 자아존중감, 자아분화수준, 자아존중감, 스마트폰 중독의 관계를 알아보기 위하여 상관관계분석을 실시하였다. 셋째, 스마트폰 중독에 영향을 미치는 자아존중감, 자아분화 수준의 영향력을 알아보기 위하여 중다회귀분석을 실시하였다.
연구결과, 첫째 전문대학생들의 자아존중감이 낮아질수록 전문대학생들의 스마트폰 중독 정도 가 높아진다는 것을 알 수 있었다. 둘째, 전문대학생들의 자아분화수준이 낮을수록 스마트폰 중독 정도가 높아진다는 것을 알 수 있다. 본 연구를 통하여 스마트폰으로 야기되는 문제들을 개선하고 건강하고 바람직한 스마트폰 사용 습관을 위한 프로그램의 기반이 되고자 한다.
본 연구는 자색옥수수 색소 1호 포엽과 속대 추출물의 항비만 활성을 검정하고자 지방분해효소 저해활성을 평가 하고 3T3-L1 지방전구세포에서 지방분화억제 효과를 검정하고자 수행되었다. Pancreatic lipase 저해 활성 결과, 색소 1호 포엽 및 속대 추출물의 100, 500, 1,000 μg/mL 농 도처리구에서 양성대조군인 orlistat 보다 높은 저해 활성을 나타내었다. 3T3-L1 지방전구세포를 배양하여 색소 1 호 포엽 및 속대 추출물의 세포독성 평가를 수행한 결과, 추출물은 모든 처리농도에서 세포 생존율에 영향을 미치 지 않은 것으로 확인되었다. 분화된 3T3-L1 지방전구세포 에서 색소 1호 포엽과 속대 추출물을 처리하지 않고 분화 시킨 대조군은 lipid droplet의 형성이 활발하게 유발되었으나 색소 1호 포엽 및 속대 추출물의 처리에 의해 농도 의존적으로 lipid droplet의 형성이 억제되는 것으로 나타났다. Real-time PCR과 Western blot을 실시하여 PPARγ와 C/EBPα 유전자 및 단백질 발현량을 측정한 결과, 추출물을 처리하지 않고 분화시킨 대조군에서는 PPARγ와 C/ EBPα의 유전자 및 단백질 발현이 증가하였으며, 추출물 처리에 의해 PPARγ와 C/EBPα의 유전자 및 단백질 발현이 유의적으로 감소하였다. 본 연구 결과는 색소 1호 포엽 및 속대 추출물이 pancreatic lipase 활성 및 지방전구 세포의 분화를 억제시킴으로써 항비만 활성 기능성 물질 로의 활용 가능성이 높음을 시사한다.
The purpose of this study was to evaluate the antioxidant and anti-adipogenic activities of Ligularia stenocephala (L. stenocephala) extract. The contents of the total polyphenol of the extract was 55.950 mg GAE/g residue. Antioxidant activities of L. stenocephala were evaluated by free radical scavenging ability and a reducing power test. 2,2'azino-bis- (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and α-α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging activities of the extract were approximately 90% and 70%, respectively. Reducing power of the extract was 258.833 mg TE/g residue. The anti-adipogenic activity of L. stenocephala extract was examined in 3T3-L1 cells. During adipocyte differentiation, the 3T3-L1 cells were treated both with and without the extract. L. stenocephala extract suppressed the lipid accumulation in a concentration-dependent manner in the 3T3-L1 cells. The L. stenocephala extract inhibited the expression of peroxisome proliferator activated receptor γ (PPARγ) and adipocyte protein 2 (aP2) proteins, compared with control adipocytes. These results indicate that L. stenocephala could be regarded as a potential source natural antioxidant and an anti-obesity agent.
Characteristics of induced pluripotent stem (iPS) cells are consistent with those of embryonic stem (ES) cells. However, exogenous genes integrated by using retrovirus delivery systems cannot be completely removed from the cells. In a recent report, activation-induced cytosine deaminase (AID) and thymine DNA glycosylase (TDG) can induce pluripotency state in mouse differentiated cells through the process of DNA demethylation. Thus, we hypothesized that the two reprogramming factors may convert efficiently bovine differentiated cells into pluripotency state. So, genes of AID and TDG were integrated into pCMV6-AC-IRES-GFP-Puro expression vector, which was transfected into bovine differentiated cells. As results, the colonies derived from AID+TDG-induced bovine cells were formed on day 7 after culture. The number of AP positively colonies in AID+TDG-induced bovine cells was significantly higher than in AID-induced bovine cells (p<0.05). Additionally, expression of pluripotent genes (OCT-3/4, NANOG, SOX2) was slightly increased in AID+TDG-induced bovine cells, as compared to AID-induced bovine cells. Protein expressions of OCT-3/4, NANOG and SOX2 in AID+TDG-induced bovine cells were slightly increased rather than AID-induced bovine cells. Finally, DNA demethylation in the promoter regions of pluripotent markers in AID+TDG-induced bovine cells was increased than that of AID-induced bovine cells. In conclusion, pluripotent stem cells could be efficiently produced from bovine differentiated cells by using non-integrating delivery system with the reprogramming factors (AID and TDG).
(-)-Epigallocatechin-3-gallate (EGCG) is a major catechin found in green tea. It is reported that EGCG possesses various health benefits including anti-cancer, antioxidant, anti-diabetes, and anti-obesity. The objective of this study was to investigate the effects of EGCG on adipogenesis via activation of AMP-activated protein kinase (AMPK) pathway in 3T3-L1 preadipocytes. In order to determine the effects of EGCG on adipogenesis, preadipocyte differentiation was induced in the presence or absence of EGCG (0~100 μM) for a period of 6 days. EGCG significantly inhibited fat accumulation and suppressed the expression of adipogenic specific proteins including peroxisome proliferator-activated receptor (PPAR)-γ. Also, EGCG markedly increased the activation of AMPK and acetyl-CoA carboxylase (ACC) and the production of intracellular reactive oxygen species (ROS). However, any pretreatment with a specific AMPK inhibitor, compound C, abolished the inhibitory effects of the EGCG on PPARγ expression. This study suggests that EGCG has anti-adipogenic effects through modulation of the AMPK signaling pathway and therefore, may be a promising antiobesity agent.
In this study, we investigated the effect of bisphosphonate on the osteoblastic differentiation of human dental stem cells (hDPSCs). In the first experiment, we evaluated the effect of bisphosphonate on the differentiation of hDPSCs into osteoblasts by alkaline phosphatase staining after culturing hDPSCs. As a result, on day 13, the osteogenic differentiation of hDPSC was suppressed at 5 μM in clodronate and 2 μM in zolendronate. In NBP, osteogenic differentiation is more suppressed. In second experiment, cytotoxicity and proliferation test, the cell proliferation (examined by MTT assay) was more suppressed as the concentrations of zolendronate were larger than those of alendronate and clodronate. Western blotting, a third experiment, was found that AKT phosphorylation was inhibited in cell signaling proteins involved in cell proliferation inhibition and death by bisphosphonate concentration. In human dental stem cells, bisphosphonates inhibit osteoblast differentiation, and this phenomenon is clearly observed in NBPs (zolendronate), and it has been found that it is related to AKT phosphorylation of cell signaling proteins.
To gain insights into the role of purinergic receptors in human dental pulp cells (hDPCs) differentiation, we characterized the expression and functional activity of P2Y1 receptors and investigated the effects of ADP on the proliferation and differentiation of this pulp stem-like cell population. Our data showed that ADP did not induce cell proliferation to expose the various ADP concentrations for 72 hours, but the proliferative capacity of hDPCs was inhibited at higher ATP concentrations (100 μM). Using RT-PCR analysis, we found that ADP induced several P2Y receptors including P2Y1 as well as odontoblastic differentiation genes, dentin matrix protein 1 (DMP1) and dentin sialophosphoprotein (DSPP) in a dose-dependent manner. The effects of ADP on the expression of DMP-1 and DSPP mRNA were prevented by the P2Y1 antagonist MRS2179. The extracellular matrix calcium deposits were clearly observed in ADP-treated hDPCs by alizarin red S staining. Quantitative measurement of mineralization induced by ADP was significantly inhibited in MRS2179-treated hDPCs. These results may provide new insights into the molecular regulation of the differentiation of hDPCs.
This study aimed to evaluate the growth of Ilex cornuta Lindl. & Paxton, Cotoneaster dammeri, Ardisia pusilla ‘Variegata’, and Ilex aquifolium 'Silver Queen' when sphagnum moss was used for media, and the pottery plants were submitted to different temperatures and shade levels. After transplantation to pottery pots with Sphagnum Moss, the growth of I. aquifolium 'Silver Queen' at 25℃ of soil was the highest with 16.8 cm in plant height, 75.0 in leaf number, 256.5 cm2 in leaf area, 18.9 g in fresh weight. I. cornuta Lindl. & Paxton and A. pusilla ‘Variegata’ were in the same range but soil temperatures of 15℃, 20℃, or 30℃ had no effect and the survival rate remained 100%. On the other hand, when I. aquifolium ‘Silver Queen’ was submitted to night temperatures of 4℃, 8℃, 12℃, or 15℃, the following results were obtained: 14.1 cm in plant height, 70.0 in number of leaf, 221.1 cm2 in leaf area, and 18.9 g in fresh weight at 15℃; C. dammeri showed similar results. The survival rate at night temperature 4℃ or 8℃ was 100%, but the plant growth was sluggish. In shading 30% or 60%, the fresh weight of I. aquifolium ‘Silver Queen’, I. cornuta Lindl. & Paxton, and A. pusilla ‘Variegata’ was increased at shading 60% as the plants became taller. Non shading increased the leaf number and leaf area of plants compared with shading. In conclusion, the quality of small woody plants in pottery can be improved when the plant is grown before shipping at a soil temperature of 25°C and a greenhouse night temperature of 15°C, at 30% shading after acclimation
This study aimed to evaluate the growth of Ilex cornuta Lindl. & Paxton, Cotoneaster dammeri, Ardisia pusilla ‘Variegata’, and Ilex aquifolium ‘Silver Queen’ when sphagnum moss was used for purpose of export and the pottery plants were submitted to different watering and quick versus slow release fertilizer. The growth of I. aquifolium 'Silver Queen' watered 50 mL every 3 days was the highest with 16.0 cm in plant height, 79.3 in leaf number, 178.2 cm2 in leaf area, and 18.5 g in fresh weight. C. dammeri also grew best when watered every 3 days. However, the survival rate was 100% for I. aquifolium 'Silver Queen' watered at 12-day intervals and C. dammeri watered at 6-day intervals or more. With quick release fertilizer of 1,000 – 2,000 mg·L-1 applied at 1- to 4-week intervals, I. aquifolium 'Silver Queen' and C. dammeri, plant height, number of leaf, leaf area, and fresh weight increased as fertilizing intervals were shorter. During the short quick release fertilizing at 4-week intervals, the survival rate remained 100%. With slow release fertilizer of 0.5 mg, 1 mg, or 2 mg applied at intervals of 6, 12, and 18 weeks, I. cornuta Lindl. & Paxton and A. pusilla ‘Variegata’ plant height, number of leaf, leaf area, and fresh weight increased with increase of fertilizer amounts and shorter intervals of fertilizing. However, concentration of fertilizer had no effect on plant growth. During the short slow release fertilizing at 18-week intervals, the survival rate remained 100%. Therefore, to improve the quality of small woody plants in the pottery aimed for export, it is preferable to water at intervals of 3 days after acclimation, and use either quick release fertilizer at 3-week intervals or slow release fertilizer at 12-week intervals.