Honey production from approximately 1.7 million colonies owned by around 21 thousand beekeepers was almost 36 thousand M/T in Korea. Pollen has used as a food and medicine from before the Joseon Dynasty period in Korea. Pollen grains such as acorn (Quercus acutissima), actinidia (Actinidia arguta) and green tea (Camellia sinensis) are popular in the markets in Korea. But stiff pollen wall hindered dissolution of polysaccharides and lowered extraction efficiency. In the present study, we measured the antioxidant activity and the total polyphenol content from the pulverized and lyophilized green tea pollen grains inoculated with 6 kinds of fungi to confirm the husk removal effect. The total polyphenol content of green tea pollens was highest in lyophilized pollen medium inoculated with Armillaria mellea, and was lowest in pollen inoculated with Lentinula edodes. Total polyphenol content of the lyophilized pollen was higher than that of the refined pollen and the pulverized pollen in green tea pollen germinated with A. mellea. The total polyphenol content of the lyophilized green tea pollens germinated with A. mellea was 1.4-fold higher than that extracted with water. Measurement of antioxidant activity using the DPPH free radical scavenging method showed that the lyophilized green tea pollen grains germinated with A. mellea had the highest and that germinated with L. edodes was lowest in antioxidant activities. The lyophilized green tea pollen grains germinated with A. mellea was 2 to 4 times higher than that extracted with water in the antioxidant activity of DPPH free radical scavenging. Many germinated cells were formed around pore of green tea pollen inoculated with L. edodes, while those were formed at the end of hyphae derived from green tea pollen grains inoculated with A. mellea.

Naringenin and its glycoside naringin (naringenin-7-O-glycoside) belong to this series of flavonoids and were well-known strong antioxidant activity. This study was conducted to flavonoid constituents and antioxidant activity of Pleurotus ostreatus and Pleurotus cornucopiae. In order to determine active ingredient contents of Pleurotus ostreatus and Pleurotus cornucopiae, we were carried out total polyphenolic content (TPC) and flavonoid content (TFC) analyses. As a result, TPC (31.05 ± 0.03 g GAE/kg extract) and TFC (13.25 ± 1.24 g NE/kg extract) of Pleurotus cornucopiae were found significantly higher as compared to Pleurotus ostreatus. The IC50 values based on the DPPH (56.50 ± 0.73 μg/ml) and ABTS (86.53 ± 3.86 μg/ml) for Pleurotus cornucopiae were generally stronger showing potential antioxidant properties compared to Pleurotus ostreatus. In addition, naringin and naringenin content were analyzed by high-performance liquid chromatography analysis. A great amount of naringin and naringenin was found in Pleurotus cornucopiae. These results indicate that the Pleurotus cornucopiae can be used as an antioxidant therapeutic agent.

The aim this study was to examine the effect of general squat and wall squat exercises on the muscle activity of the vastus medialis oblique (VMO) and vastus lateralis (VL), and the muscle activity of the VMO and VL during squat exercises depending on the flexion angle of the knee joint. Thirty adults in their 20s participated in the study, and subjects were divided into the general squat group, and the wall squat group, and their electromyographic (EMG) was monitored and collected during squat exercises. The muscle activity of the VL during squat exercises was compared between the two groups, and that of the wall squat group was higher than that of the general squat group, indicate a statistically significant difference (p<.05). The muscle activity of the VMO of the wall squat group was also higher than that of the general squat group, indicate a statistically significant difference (p<.05). The muscle activity depending on the angle of the knee joint was compared between the two groups, and that of the VL showed a statistically significant difference between different angles of the knee joint (p<.05). That of the VMO also showed a statistically significant difference between different angles of the knee joint (p<.05). The findings of the present study suggest that wall squat is more effective in improving the muscular strength of the VL and VMO than general squat, and that maintaining the angle of the knee joint at 60° is more effective in improving the muscular strength than maintaining it at 15° or 45°

The aim of the present study was to investigate the effects of unilateral shoulder bag weight and heel heights on trunk muscle activities. This study recruited 40 healthy women in their twenties. Electromyography activities of rectus abdominis muscle, external abdominal oblique muscle, internal abdominal oblique muscle and erector spinae muscle during standing position were measured using a surface electromyography system. The overall muscle activities of the trunk muscles were significantly increased in the contralateral trunk muscles (p<.05). Inaddition, the trunk muscles overall activities on the contralateral side without the shoulder bag was positive correlation with the heel height and unilateral shoulder bag weight (p<.05). Both high-heeled wear and unilateral shoulder bags are fashion items that cause asymmetry in the trunk muscles of women in their twenties. These findings suggest that the increase in the weight and heel height of the unilateral shoulder bag in women cause asymmetry of trunk muscle activities.

The purpose of this study was to examine the effects of abdominal hollowing with ultrasound biofeedback on multifidus and transversus abdominis/internal oblique muscle activities during lifting tasks. This study recruited 12 healthy men. All subjects received training on expiration without and with abdominal hollowing with ultrasound biofeedback. After receiving training for each condition, the thickness of transversus abdominis was measured using ultrasound, and then, electromyography activities of transversus abdominis/internal oblique and multifidus during lifting tasks were measured using a surface electromyography system. The differences in thickness of transversus abdominis and electromyography activities of transversus abdominis/ internal oblique and multifidus between the two conditions were analyzed using paired t-test. Thickness of transversus abdominis and transversus abdominis/internal oblique and multifidus activities were significantly increased during expiration with abdominal hollowing with ultrasound biofeedback compared to during expiration without abdominal hollowing (p < 0.05). These findings suggest that abdominal hollowing with ultrasound biofeedback is effective in increasing trunk stabilizer muscle activities during both static and dynamic tasks such as lifting.

Background: Given the potential benefits of wellness programs promoting physical activity of the community-dwelling frail elderly, it is recommended that comprehensive wellness programs combined with the component of physical activity. This may improve overall health and potentially lower the health care cost of the frail elderly. In general, the frail elderly residing in community or those after being discharged from hospitals are often committed to enhancing the status of participation in physical activity. Objects: The purposes of this study were to identify specific shortcomings of current wellness programs as part of continuum of community rehabilitation services and to propose alternatives for the care of the community-dwelling frail elderly. Through this study, geriatric health care professionals may be able to implement assessments and programs to successfully promote an effective continuum of care for the frail elderly. Methods: Article reviews were summarized and evaluated. Results: A model elaborating the relationship between components of successful wellness program and participation in physical activities for the community-dwelling frail elderly are recommended. First, periodic monitoring the levels of physical activity by the use of online measurement system should be considered. Second, individualized adaptive technologies for selecting optimal physical activities for the elderly may be better fit to individuals’ current status of physical activity. Conclusion: The current status of physical activity in community-dwelling frail elderly can be monitored by online assessment systems. Through the innovative measurement system, elderly may assess his/her physical activity status overtime, select optimal physical activities matching the status, and create the elderly’s own adaptive wellness programs that match to the status while residing in his/her community.

식물 및 동물성 단백질 유래 펩타이드 형태의 단백질 가수 분해물들은 항산화, 고혈압 완화, 면역조절, 진통완화 및 항균작용 등 생리활성이 있는 것으로 알려져 왔다. 본 연구는 연산오계란 단백질 가수 분해물을 Ultrafiltration를 이용하여 HDS(분획되지 않은 가수 분해물), 1 kDa, 5 kDa, 10 kDa, 50 kDa로 분획된 기능성 펩타이드의 DPPH radical scavenging activity, superoxide anion radical scavenging activity, hydroxyl radical scavenging activity 및 Fe2+ chelation ability을 평가하였다. 그 결 과 DPPH radical scavenging activity 최대값은 1 kDa(70.83 %), hydroxyl radical scavenging activity 최대값은 5 kDa (47.01 %), superoxide anion radical scavenging activity 최대값은 5 kDa(40.57 %), Fe2+ chelation ability 최대값은 5 kDa(29.87 %)로 나타났다. Ultrafiltration를 이용하여 fractionation된 단백질 가수 분해물의 항산화 저해 능력 IC50 평가하였다. 그 결과 HDS의 최대값은 superoxide anion radical scavenging activity(IC50, 5.42 mg/ml)이고, 1 kDa의 최대값은 Fe2+ chelation ability(IC50, 1.67 mg/ml)이고, 5 kDa의 최대값은 Fe2+ chelation ability(IC50, 2.09 mg/ml)이고, 10 kDa의 최대값은 Fe2+ chelation ability(IC50, 2.61 mg/ml)이고, 50 kDa의 최대값은 Fe2+ chelation ability(IC50, 4.53 mg/ml)이 다. 그러므로 본 연구 결과를 바탕으로 5 kDa를 이용하여 오계란 단백질에서 분획한 펩타이드는 항산 화 기능성 식품소재로서 활용할 가치가 높을 것으로 기대한다.

식물 및 동물성 단백질 유래 펩타이드 형태의 단백질 가수 분해물들은 항산화, 고혈압 완화, 면역조절, 진통완화 및 항균작용 등 생리활성이 있는 것으로 알려져 왔다. 본 연구는 6가지 프로티아제 를 이용하여 오계란 단백질 가수분해물을 생산하고, 생산된 펩타이드의 항산화 능력을 평가하였다. 그 결과 가수분해도의 최대값은 protamex(46.3%)이고, DPPH 라디칼 소거능 최대값은 bromelain(57.23%), 하이드록시 라디칼 소거능 최대값은 alcalase(30.21%), 슈퍼옥사이드 라디칼 소거능 최대값은 alcalase(58.07%), Fe2+ 킬레이션 능력 최대값은 alcalase(72.06%)로 나타났다. 더 나아가 효소별 항산화 저해 능력 IC50 평가하였다. 그 결과 alcalase에 의한 최대값은 금속 킬레이션 눙력(IC50, 1.24 mg/mL) 이고, bromelain에 의한 최대값은 DPPH 소거능(IC50, 2.46 mg/mL)이고, flavourzyme에 의한 최대값은 금속 킬레이션 능력(IC50, 1.25 mg/mL)이고, neutrase에 의한 최대값은 DPPH 소거능(IC50, 3.64 mg/mL)이고, papain에 의한 최대값은 DPPH 소거능(IC50, 3.82 mg/mL)이고, protamex에 의한 최대값 은 DPPH 소거능(IC50, 1.93 mg/mL)이었다. 따라서 protease를 이용하여 오계란 단백질에서 추출한 펩 타이드는 항산화 기능성 식품소재로서 활용할 가치가 높을 것으로 기대한다.

붉나무의 유용성에 대한 기초자료를 마련하고자 붉나무 수피를 에탄올로 추출하여 추출물의 몇 가지 이화학적 특성과 항산화 활성을 측정하였다. 추출물의 가용성 고형분의 함량은 73.5 mg/100g, 당도 는 17.8 °Brix로 측정되었으며, 총방향족 화합물, 총플라보노이드 함량 및 총페놀성 화합물의 함량은 각각 흡광도 0.508, 49.88 mg/100g 및 296.6 mg/100g으로 측정되었다. 추출물의 환원력은 대조구로서 추출물 의 가용성 고형분과 동량의 ascorbic acid의 27.5 %로 측정되었으나, 철환원력은 ascorbic acid 와 유사하 게 측정되었다. 추출물의 DPPH라디칼 소거능은 95.16 %로서 ascorbic acid와 거의 유사하였고, 금속이온 봉쇄능은 ascorbic acid의 74.73 %보다 높은 81.58 %로 측정되었다. 그리고 pH 2에서 추출물의 아질산염 소거능은 51.76 %로 측정되었으며, Rancimat test에 의한 대두유 산화 억제효과가 있음을 확인하였다.

생태계 교란 식물로 알려진 가시박 추출물을 활용하여 생리 활성 효과를 알아보고, 기능성 화장품 소재로써의 활용 가능성을 확인하고자 하였다. 본 실험 방법으로는, 총 폴리페놀 함량, 총 플라 보노이드 함량을 측정하고, DPPH radical 소거능, 세포 내 ROS 생성 억제, NO 및 COX-2 발현 억제 효과를 평가하고자 하였다. 본 실험 결과, 가시박 추출물의 21 mg/g, 0.72 mg/g의 총 폴리페놀과 총 플라보노이드 함량을 확인하였으며, 높은 라디칼 소거 활성을 통한 항산화 활성을 확인 하였다. RAW 264.7세포와 HDF세포에서 100 μg/mL 농도까지 유의한 세포 독성은 확인되지 않았으며, HDF세포에 서의 농도 의존적인 ROS 생성 억제와 RAW 264.7 세포에서의 높은 NO 생성 억제와 COX-2 단백질 발현 억제 효과를 확인 하였다. 이와 같은 결과를 통하여 항산화 효과가 우수하고, 세포 내 ROS 생성 억제와 NO 생성 억제, COX-2 단백질 발현 억제 활성을 통하여 항산화 및 항염증의 효과를 가진 기 능성 화장품 소재로써의 활용 가능성을 확인 하였다.

The life and astronomical activity of Lee Deok-Seong (李德星, 1720-1794) was studied using various historical sources, including the astronomical almanac, Seungjeongwon-Ilgi (Daily records of Royal Secretariat of Joseon dynasty), and the Gwansang-Gam’s logbooks during Joseon dynasty (A.D. 1392– 1910). We present the results of the study including the following main findings. First, from the investigation of Lee’s family tree, we find that a number of his relatives were also astronomers, notably Samryeok-Gwan (三曆官, the post of calendrical calculation). Second, we find that he took part in the compilation of an annual astronomical almanac over a period of at least 16 years. His major achievements in the astronomy of the Joseon dynasty were to establish a new method of calendar-making calculation and to bring astronomical materials to the Joseon court through a visit to China. The Joseon dynasty enforced the Shixianli (時憲曆, a Chinese calendar made by Adam Shall) in 1654 without fully understanding the calendar. So an astronomer and an envoy were dispatched to China in order to master the intricacies of the calendar and to learn as much of Western science as was available in that time and place. Lee Deok-Seong worked at the Gwansang-Gam (觀象監, Royal Astronomical Bureau) during the reigns of King Yeongjo (英祖) and Jeongjo (正祖). As best as we can ascertain in relation with the calculations in the Shixian calendar, Lee visited China four times. During his trips and interactions, he learned a new method for calendar-making calculations, and introduced many Western-Chinese astronomical books to Joseon academia. Lee greatly improved the accuracy of calendrical calculations, even while simplifying the calculation process. With these achievements, he finally was promoted to the title of Sungrok-Daebu (崇祿 大夫), the third highest grade of royal official. In conclusion, history demonstrates that Lee Deok-Seong was one of the most outstanding astronomers in the late-Joseon dynasty.

풀무치 (Locusta migratoria) (Orthoptrea: Acridiade)는 전 세계적으로 작물 생산에 심각한 문제를 야기하는 돌발 해충이다. 그러나 우리나라 의 경우 풀무치를 방제하기 위한 방제제 및 적용에 대한 연구는 미흡한 실정이다. 본 연구에서는 풀무치에 병원성을 갖는 풀무치병원성 진균 라이브러 리를 구축하였으며, 풀무치 방제에 이용 가능한 생물학적 방제제로서의 가능성을 평가 하였다. 먼저 갈색거저리 유충-baiting 시스템을 이용하여 다양 한 지역에서 채집된 토양에서 곤충병원성 진균을 발굴 하였다. 풀무치 병원성 검정을 진행하기 위하여 국립 농업 과학원에서 풀무치를 분양 받았으며, 고체 배양된 곤충병원성 진균을 곤충 사육 상자에 처리하여 (2 g/ box), 풀무치 약충 (3-4령충)에 대한 곤충병원성 진균의 병원성을 평가 하였다. 그 결과 곤충병원성 진균 처리 3-7일차에 풀무치의 머리, 복부, 다리 표면에서 진균이 증식하는 mycosis를 확인 할 수 있었다. 특히, Metarhizium anisopliae, M. lepidiotae, Clonostachys rogersoniana에서 높은 병원성이 나타나는 것이 확인 되었다. 확보된 34개의 풀무치병원성균주의 특성을 파악 하기 위하여 열안정성 및 포자생산성을 확인 한 결과, Paecilomyces, Purpureocillium 균주가 다른 균주에 비해 열에 대한 높은 안정성안 나타나는 것을 확인 하였으며, 대부분의 균주에서 1 × 108 conidia/gram 이상의 포자수를 생산 하는 것을 확인 하였다. 또한 온실 조건에서 비교적 병원성이 높았던 M. anisopliae 고체 배양된 균주를 토양에 처리하여 병원성을 확인한 결과, 85.7%의 높은 방제효과를 확인 할 수 있었다. 본 실험을 통하여 풀무치가 이동하면서 토양에 정착된 곤충병원성 진균에 접촉되어 치사 될 수 있을 것으로 판단되며, 효과적인 풀무치 방제가 가능 할 것이라고 판단된다.

핵종재고량 관리는 처분시설의 안전한 관리를 위해서는 필수적이다. 본 논문에서는 원자력발전소의 잡고체폐기물에 대하 여 기존 발생된 폐기물 자료를 반영한 예측방사능량과 실제 처분시설을 운영하면서 인수되어 처분검사까지 완료된 실제방 사능량을 비교분석하였다. 극저준위방사성폐기물에서는 137CS, 90Sr, 99Tc 그리고 129I 핵종이 예측방사능량보다 실제방사능량 이 높게 평가되었으며, 저준위방사성폐기물에서는 모든 핵종에서 예측방사능량이 실제 방사능량보다 높게 평가되었다. 또 한 척도인자에 의한 예측방사능량의 민감도 분석을 통해 준위별 수량 및 총방사능량의 변화추이를 분석하였다. 향후 중저준 위방사성폐기물 처분시설의 안전한 운영과 Safety Case 구축을 위한 기초자료로 활용될 것으로 판단된다.

Senior citizens are reported to be highly sensitive to environmental pollutants, including fine particulate matter. The purpose of this study is to investigate the distributions of fine particulate matter concentrations in town halls and senior citizens’ halls, where senior citizens typically spend a lot of time. The results of this investigation will serve as fundamental data for conducting indoor air quality studies and establishing a regional environmental health policy governing senior citizens’ facilities in Yeongwol county in the future. From January 4 to February 1, 2017, PM10 and PM2.5 concentrations in 170 seniors’ facilities located in Yeongwol county were measured. The average concentrations of PM10 and PM2.5 that were measured from each seniors’ facility chosen from Yeongwol county were 25.1 ± 18.9 μg/m3and 12.5 ± 9.3 μg/m3 respectively. Average concentrations and the average of maximum concentrations were lower than the living space standard of 100 μg/m3 for PM10 and the atmospheric environment standard of 50 μg/m3 for PM2.5, which were set by the Ministry of Environment for populations vulnerable to environmental pollution. As a result of analyzing the sources in seniors’ facilities in Yeongwol county during the winter months, it was found that indoor sources of air pollution such as cooking is main sources rather than outdoor sources of air pollution.

Proximate analysis and antioxidant activity of cultivated wild Panax ginseng (CWPG) were investigated to provide fundamental information of CWPG with different ages and to increase its industrial application. Proximate analyses of CWPG with different ages were performed. Extraction of CWPG with different ages was carried out using heatreflux extraction, and their extraction yield, crude saponin content, ginsenoside content, and antioxidant activity were analyzed. Moisture content decreased, but crude fat and crude protein were increased with aging. Extraction yield and crude saponin contents did not show a specific pattern while 5-year-old CWPG revealed the highest extraction yield and crude saponin content. All CWPGs showed typical ginsenoside profiles containing C-K and Rh2 ginsenosides, which are not found in ginseng. The 3-year-old CWPG showed the highest antioxidant activity including total phenolic content, total flavonoid content, and DPPH and ABTS radical scavenging activities. Moreover, 3-year-old CWPG also revealed the highest acidic polysaccharide content. Therefore, these results suggested that 3-year-old CWPG, which is the cheapest, can be used in industrial application due to its high antioxidant activity and acidic polysaccharide content with similar ginsenoside profile compared to 5- and 7-year-old CWPGs.

This study is an empirical study on the relationships among success factors of six sigma(support of top management, education and training, performance-based reward, customer and quality focused thought) on change management activity and business performance. In particular, mediating effect of change management activity on the relationship between success factors of six sigma and business performance is empirically analysed to find causes and impacts among these variables. The study results are as follows: First, there were positive relationships between critical success factors of six sigma(except support of top management) and change management activity. Second, there was also a positive relationship between change management activity and business performance. Third, there were also positive relationships between critical success factors of six sigma and business performance. Finally, change management activity was partial mediating effects on the relationship between critical success factors of six sigma(education and training, performance-based reward, customer and quality focused thought) and business performance. On the other hand, support of top management had no mediating effect on business performance.

The formation mechanism and photocatalytic properties of a multiwalled carbon nanotube (MWCNT)/TiO2- based nanotube (TNTs) composite are investigated. The CNT/TNT composite is synthesized via a solution chemical route. It is confirmed that this 1-D nanotube composite has a core-shell nanotubular structure, where the TNT surrounds the CNT core. The photocatalytic activity investigated based on the methylene blue degradation test is superior to that of with pure TNT. The CNTs play two important roles in enhancing the photocatalytic activity. One is to act as a template to form the core-shell structure while titanate nanosheets are converted into nanotubes. The other is to act as an electron reservoir that facilitates charge separation and electron transfer from the TNT, thus decreasing the electronhole recombination efficiency.

To increase the shelf-life of strawberry, samples were treated with two gel packs containing slow-released chlorine dioxide (ClO2) gas at 3~5 ppm for 6 days at room temperature and the changes in the major chemical components (ascorbic acid, polyphenols, flavonoids and anthocyanin) contents and antioxidative activities (DPPH, ABTS radical scavenging and metal chelating activity) were investigated. The content of ascorbic acid of control was 40.38 mg% and contained 35.67~44.75 mg% during 6 days. There was no tendency to increase or decrease during storage period. The contents of ascorbic acid of control and 3~5 ppm ClO2 gas treated samples were not significantly different during storage period. The content of polyphenol compounds of initial stage was 111.23 mg% and contained 117.78~132.40 mg% during 6 days. The contents of polyphenol compounds of 3~5 ppm ClO2 gas treated samples were 103.51~130.25 mg%. There were no significant different between them during storage. The flavonoids and anthocyanin contents were not different from the control during storage period regardless of 3~5 ppm ClO2 gas treatment. Furthermore, antioxidative activities were not different among the control and ClO2 gas treatments during storage.

The purpose of this study was to investigate and analyze the total phenolic content (TPC), antioxidant activities (FRAP, ABTS, and DPPH), and antibacterial properties of lotus (Nelumbo nucifera) extracts. Lotus leaves, stems, and seed pods were extracted with deionized water at 95℃, and with 70.5% ethanol at 85℃. The TPC ranged from 8.12 to 215.12 GAE mg/g. The ethanol extract of the seed pod had the highest TPC, and the TPC of the corresponding deionized water extract was 161.45 mg/g. FRAP values ranged from 104.03 to 3,546.39 TEAC μmol/g, ABTS radical cation scavenging activities ranged from 105.11 to 3,956.94 TEAC μmol/g, and DPPH radical scavenging activities ranged from 37.29 to 2,549.46 TEAC μmol/g. EC50 values ranged from 0.26 to 9.63 mg/mL, and 0.31 to 21.21 mg/mL for ABTS and DPPH, respectively. The ethanol and deionized water extracts of the seed pod showed higher TPC and stronger antioxidant properties (FRAP, ABTS, and DPPH) than those of characteristic of the leaf extracts. The ethanol and deionized water extracts of the seed pod showed antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, and Pseudomonas aeruginosa with inhibition zones of 9.0 to 14.0 mm, and the ethanol extract of the leaf showed antimicrobial activity against B. subtilis and S. aureus with inhibition zones of 9.0 and 10.0 mm, respectively. Thus, the lotus seed pod could be used to produce novel teas, and could be a potential source of therapeutic ingredients for food and medicine.

This research aimed to improve the healthy properties of puffer fish broth, which has been utilized in Korean and Japanese food. Various healthy foods such as garlic, onion, mushroom, and cauliflower were added as ingredients to pufffer fish stock, and the antioxidative activity of each stock was measured by assaying the DPPH and ABTS radical scavenging activities, reducing power and amount of polyphenol. Shiitake was the most effective in increasing the antioxidative activity of puffer fish stock. The high antioxidative activity of shiitake mushroom seems to be correlated with the amount of polyphenol content in puffer fish broth. The antioxidant activities of puffer fish stock increased proportionally with increasing amount of added shiitake, which in turn was due to the increased amount of total polyphenol in the stock.