This study was conducted to select plant growth regulators effective at ginseng berry set inhibition to help root growth in Korean ginseng (Panax ginseng C. A. Meyer). PGRs (ethephon, gibberellic acid, maleic hydrazide, coumarin) were applied to field grown 5-year-old Korean ginseng between one and two times, before and during bloom in 2009, 2010. The number of treatment was more effective in ginseng berry set inhibition when used two times compared with one time in GA 1,000 ppm, MH (5,000, 10,000 ppm), coumarin (5,000, 10,000 ppm) treatment. According to treatment period of plant growth regulator, ginseng berry set inhibition rate from 20days before flowering date to 5days after blooming was the highest in MH 5000 ppm showing 99.9% and the lowest in GA 100 ppm showing 32.8%. The spray treatments of Ethephon (50, 150 ppm) and MH (5,000, 10,000 ppm) from 20 days before the flowering bloom up to 5 days before, and coumarin (5,000, 10,000 ppm) from 20 days to 6 days and before blooming that induced the inhibitory effect more than 90% after 12 weeks. Considering ginseng berry set inhibition characteristics and treatment period ethephon and coumarin was important about applied period but, MH treatment appeared to effective ginseng berry set inhibition regardless of treatment period.

들깻잎 생산 전용품종은 대부분 만생종으로서 우량종자를 채종하고자 할 경우 도복 등이 발생하지 않아 생육관리가 용이하면서 종실 충실도가 높은 파종적기를 설정할 필요가 있다. 이에 중부지역 비닐하우스에서 잎들깨 생산 전용 품종 '잎들깨 1호'를 시험재료로 하여 5월 6일부터 7월 15일 까지 10일 간격으로 8회 파종하여 생육, 도복 발생 여부, 적산온도 및 수량성을 분석하였다. 1. 파종시기가 늦을수록 경장이 짧아지고, 주경절수, 분지수, 화방군수와 화방당 삭수는 감소되었다. 그러나 파종시기가 빠를수록 지상부 생육이 왕성하여 도복이 발생하였다. 2. 개화일수는 파종시기가 늦어질수록 직선적으로 감소되었는데 5월 6일을 기준으로 파종이 1일 지연되면 개화일수는 0.86일씩 단축되었고, 개화기부터 성숙기까지의 결실일수는 29~31일로 파종기 이동에 따른 차이가 없었다. 3. 등숙립율과 종실수량은 5월 6일 파종부터 6월 15일 파종까지 유의성이 없었고, 6월 25일 파종 이후부터 급격히 감소되었다. 4. 등숙에 필요한 적산온도, 도복 발생 여부, 등숙립율과 발아율 등으로 판단하면 만생종 잎들깨 품종의 중부지역 비닐하우스에서 파종적기는 6월 5일(정식 7월 5일)~6월 15일(정식 7월 15일)이었고, 이때 10월 28일~11월 3일경에 성숙되어 종자생산이 가능하였다. 5. 연차에 따라 0℃ 이하로 떨어지는 시기가 일찍 오는 경우 한해에 의하여 노지재배는 고사될 우려가 있고, 중부지역에서는 비닐하우스를 이용한 잎들깨 채종재배가 가능하였다.

본 연구에서는 종실의 크기가 작고 불균일한 소립종으로 파종시 초기 입모불량의 문제점을 개선하기위해 형질특성이 다른 밀수형 황금찰와 산수형 토종 품종을 대상으로 종자크기를 5가지 등급으로 선별하여 실내종자활력 평가와 포장조건에서 선별파종 효과를 비교 분석한 결과 다음과 같은 결론을 얻었다. 주요 수수품종별 종자의 크기별 분포특성을 분석한 결과 황금찰에 비해 토종이 대립종으로 나타났으며, 종자의 균일도도 더 높은 것으로 나타났다. 종자크기가 클수록 천립중과 비중, 탄수화물과 단백질 함량이 증가되었고, >3.55 mm 와 <2.36 mm의 천립중도 황금찰 19.78 g과 토종 17.91 g로 차이를 보였으며, 탄수화물과 단백질함량은 각각 0.98%, 14.61%의 차이를 보였다. 종자크기 >3.55 mm와 3.15 mm 범위에서는 탄수화물과 단백질함량 함량은 유의성이 없었으며, 탄수화물/단백질함량비도 비슷한 경향을 보였다. 종자활력을 시험한 결과 저온과 표준발아시험 모두 종자 크기가 클수록 발아율이 높은 것으로 나타났으며 종자크기가 >3.55 mm 이상의 종자 발아율이 79.8%로 높은 반면<2.36 mm에서는 51.5%로 크게 감소되는 경향을 보였으며, 2.80 mm 이하부터는 크게 떨어졌다. 포장조건에서 출아율은 황금찰의 경우 >3.55 mm에서는 92.7%로 높은 반면 <2.36 mm에서는 31.3%로 크게 낮았다. 평균출아일수는 황금찰의 경우 >3.55 mm에서는 4.26일이 소요되는 반면 <2.36 mm에서는 4.74일이 소요 되었고, 출아율지수는 >3.55 mm에서는 0.87인 반면 <2.36 mm에서는 0.26으로 종자크기가 작을수록 크게 감소되는 경향을 보였다. 수량에서는 <2.36 mm에 비해 >3.55 mm에서 황금찰 24.5%, 토종 36.9% 증가하는 경향을 보였으며 특히 3.15 mm 미만부터는 뚜렷한 수량성의 차이를 나타났다. 따라서 수수종자 크기별로 중량, 밀도, 성분분석, 표준발아 조사, 저온발아조사 등 실내 종자활력 시험과, 포장조건에서 발아시험을 수행한 결과를 종합하였을 때 3.15 mm 이상의 종자를 선별하여 파종하면 수수의 종자활력이 높아져 생산성이 크게 향상되는 것으로 판단되었다.

Leptoglossus occidentalis Heidemann, 1910 was newly recorded in Korea as a invasive species at Gyeongsangnam-do, Korea in 2010 and 2011. Leptoglossus Guérin-Méneville, 1831 is also newly recorded Coreid genus in Korea. The diagnostic shape is expanded leaf-like hind tibiae. The origin of this species is North America and it has been rapidly propagated around the world as a pest. This bug recognized as a pest on almost of Pinaceae plants and they suck on developing cones, so the monitoring on the present invasion of this species in Korea is urgent. It was presumed that the invasion of Leptoglossus occidentalis into Korea was before 2010 with present study.

국내 유일 설악산에만 분포하는 희귀수종 눈잣나무의 현지외 보존을 위한 종자수집에 따른 구과보호망의 효과와 수집된 구과와 종자의 형태적 특성 변이를 조사하였다. 철망으로 설치된 구과보호망(50 mesh, 25 × 25 cm)은 설치류나 조류 등의 피해를 방지할 수 있으며 안정적인 종자유전자원 확보가 가능한 것으로 나타났다. 특히, 7월 초순부터 설치하는 것이 처리효과가 매우 높은 것으로 나타났다. 눈잣나무 구과는 열개하지 않는 폐과이며 종자에는 날개가 없고 주로 잣까마귀와 설치류 등의 먹이저장 습성에 의해 종자가 산포된다. 구과의 형태적 특성 변이는 개체간 유의적인 차이가 인정되었으며, 평균특성은 구과길이 35.3 mm, 구과폭 25.6 mm, 구과지수 1.38, 구과건중 6.6 g, 구과당 종자수 39.3립을 각각 나타냈다. 종자는 삼각모양의 달걀형으로 날개가 없으며 개체간 유의적인 차이가 인정되었다. 평균특성은 종자길이 8.12 mm, 종자폭 5.81 mm, 종자지수 1.40,종자두께 4.62 mm, 종자무게 0.11 g을 각각 나타냈다. 변이계수 값은 구과건중과 구과당 종자수에서만 비교적 높은 21.7%와 21.5%를 각각 나타냈다. 구과와 종자특성 상관분석에서는 구과와 종자의 길이가 길어질수록 전체적인 크기가 커지고 모양이 길어지며, 무게도 많이 나가는 경향을 나타냈으며, 또한 종자폭이 넓고 종자두께가 두꺼울수록 구과당 종자수는 작아지는 부의 상관관계를 나타냈다.

Soyasaponin group B함량의 재배지대간 비교에서는 고랭지와 평난지에서 상이한 차이를 보였으며 품종 간에는 대부분 자엽색이 녹색이고 종피색이 검정색인 콩의 soyasaponin group B함량이 높은 것으로 나타났다. 이상의 결과를 종합해 볼 때 soyasaponin성분의 함량은 재배종의 종류 즉 유전적인 요인뿐만 아니라 고랭지와 평난지처럼 환경적 요인이라고 것을 확인할 수 있었으며 이는 식물이 환경적인 스트레스에 대한 생명유지 및 공격방어물질(Secondary matabolites, 이차대사산물)이 식물내 생합성과정 중에서 변이를 보이는 것으로 사료되어 진다.

Grain yield, one of the most important agronomic traits, is greatly affected by architecture in rice. Here, we show that an OsPrMC3, a rice PrMC3 orthologue with a lipase or esterase domain, involves in yielding by tillering. Phenotypic analysis of T-DNA insertion mutant revealed that it has high number of tillers than wild type although height and leaf width are shorter and narrower than wild type. Size and branch number of panicle were greatly reduced in the mutant, which resulted in significant decrease of seed number per panicle and dry weight of the seeds. OsPrMC3 is highly expressed in the leaf during the early stage of development. However, it is mainly expressed in mature seed and root after flowering although its expression is detected in all of the tissues. Our result indicates that OsPrMC3 involves in leaf growth and tillering during vegetative growth and also seed development after flowering, suggesting its crucial regulatory role in yielding

The surveying of binding affinity between a particular transcription factor and DNA motifs is important in order to understand the developmental specific gene expression and regulatory networks of an organism. The microarray-based technologies (protein-binding microarrays; PBMs) provide useful predictions for understanding the transcriptional regulatory code in a genome-wide manner. The PBM was designed in such a way that target probes were synthesized as quadruples of all possible 9-mer combinations, named Q9-UPBM. Also, we developed rice promoter PBM (RPBM) using 19,480 rice promoter sequences containing 40 bp long probe with overlapping 20 bp (cover 1kb from 5’ upstream). We applied RISBZ1 protein, an endosperm specific basic leucine zipper transcription factor, to compare binding site specificities between Q9-UPBM and RPBM and find directly regulated promoter regions through the RPBM. Several cis-elements; Prolamin box (TGTAAAG), GCN4 motif (TGA(G/C)TCA), AACA motif (AACAAAA), and ACGT motif, are highly conserved in the promoters of cereal seed storage protein genes, and play a central role in controlling endosperm specific expression during seed maturation. Characterization of cis-elements and TFs has been performed on many storage protein genes of several crop plants, but the mechanisms are still poorly understood. Two chips provide RISBZ1 could bind to ACGT motif such as a CCACGTCA site and GGATGAC site as well as GCN4 motif known binding site. In RPBM binding affinity to CCACGTCA was highly significant, compared to GGATGAC site. The difference might be caused by the biased presence of specific promoter rather than Q9-UPBM. Also our results will provide direct insight into the importance of combinatorial interplay between cis-elements in regulating the expression of seed storage protein genes.

Phomopsis seed decay (PSD), primarily caused by Phomopsis longicolla, is a major contributor to poor soybean seed quality and significant yield loss, particularly in early maturing soybean genotypes. However, it is not yet known whether PSD resistance is associated with early maturity. This study was conducted to identify quantitative trait loci (QTLs) for resistance to PSD and maturity time using a recombinant inbred line (RIL) population derived from a cross between the PSD-resistant Taekwangkong and the PSD-susceptible SS2-2. Based on a genetic linkage map incorporating 117 simple sequence repeat markers, QTL analysis revealed two and three QTLs conferring PSD resistance and maturity time, respectively, in the RIL population. Two QTLs (PSD-6-1 and PSD-10-2) for PSD resistance were identified in the intervals of Satt100-Satt460 and Sat_038-Satt243 on chromosomes (Chrs) 6 and 10, respectively. These QTLs do not overlap with any previously reported loci for PSD resistance in other soybean genotypes. Two QTLs explained phenotypic variances in PSD resistance of 46.3% and 14.1%, respectively. Among three QTLs for maturity time, two (Mat-6-2 and Mat-10-3) were located at positions similar to the PSD resistance QTLs. The identification of the QTLs linked to both PSD resistance and maturity time indicates a biological correlation between these two traits. The newly identified QTLs for resistance to PSD associated with maturity time in Taekwangkong will help improve soybean resistance to P. longicolla.

Soybean seed contains a wide range of secondary metabolite compounds such as isoflavones, phyto-sterols, lecithins and saponins. The secondary metabolites are diverse in chemical structure and property. Therefore, it is not easy to analyze simultaneously the diverse metabolites. We assessed LC-MS profiling analysis to evaluate seed component diversity in 33 soybean cultivars and to identify diverse substances according to their fragmentation patterns. The 33 cultivars were divided clearly into two groups according to PCA of the profile data of seed components. The soluble extracts from hypocotyle as well as cotyledon in Group 1 were characterized by the presence of a compound with 969.5 m/z, while the extracts in Group 2 were characterized by the presence of a compound with 980.6 m/z. The two cultivars Williams 82 and Enrei were selected from each group, and then subjected to further analyses. PMF (Peptide MS Fingerprint) data generated by the Q-TOF analysis and MASCOT database search identified the compounds composed of 37 amino acids as the 4-kDa peptide (Albumin 1b). Substitution of three amino acids was found between the two groups. Three candidate genomic sequences were distributed on soybean genome. Expression analysis by RT-PCR indicated one of the three sequences encodes the 4-kDa peptide and expressed in developing seed. In this study, we confirmed the comprehensive analysis with LC-MS is a powerful tool to elucidate metabolite diversity in plant materials including soybean seed.

Gene expression profiles can serve as a valuable reference for deciphering gene functions. We exploited the potential of whole genome microarrays to measure the temporal expression profiles of rice genes in 13 stages of reproductive development. We could profile expression of 17,676 genes in at least one of the tissues. Differential expression analysis with compare to leaf and preceding stages of development revealed reproductive stage-preferential/-specific genes. we identified 35 genes expressing specifically during panicle and seed development. The metabolic/hormonal pathways and transcription factor families playing key role in reproductive development were elucidated after overlaying the expression data on the public databases and manually curated list of transcription factors, respectively. During floral meristem differentiation (P1cm) and male meiosis (P5cm), the genes involved in jasmonic acid and gebbellin biosynthesis were significantly upregulated. F11DAP stage of seed, containing enlargement organ, exhibited enrichment of transcripts involved in starch or sucrose biosynthesis. Genes regulating auxin biosynthesis were induced during early seed development. We validated the stage-specificity of regulatory regions of two panicle-specific genes, AK072471, Os08g0538700, and AK121412, an early seed-specific gene, in transgenic rice. The data generated here provides a snapshot of the underlying complexity of the gene networks regulating rice reproductive development.

Kenaf (Hibiscus cannabinus) is an annual herbaceous plant of the family Malvaceae that has been planted in Africa for more than 4000 years and used as source of fiber, energy and feed stock. Also, kenaf seeds are good source for edible oil used for first class cooking oil and margarine production. The seeds can be used for lubrication, soap, paint and varnishes. This study was carried out to evaluate fatty acids variation among sixteen kenaf germplasm and gamma-ray induced mutants derived from Jinju and Auxu. Linoleic, oleic, and palmitic acid were the predominant fatty acids in all kenaf seed oils. The sixteen accessions showed a wide range of fatty acid compositions, spanning from 28.94 to 43.36% saturated, 56.64 to 71.05% total unsaturated, 15.52 to 46.85% monounsaturated, and 13.56 to 48.97% polyunsaturated fatty acids. The mutant lines derived from Jinju, significantly surpassed parental mean for all the palmitic and oleic acid. Also, the mutant lines derived from Auxu showed broad ranges of variation in oleic and linoleic acid and narrow ranges of variation in stearic and palmitic acid. The relative amount of monounsaturated fatty acids (MUFA) were increased at all the gamma-ray induced mutants. These results will provide a valuable information to assist parental selection of kenaf breeding.

Soybean [Glycine max (L.) Merr.] protein is excellent nutritional factors and is widely used for human and animal feed in the world. Glycinin(11S globulin) and ß-conglycinin(7S globulin) account for storage protein from 70% to 80% in soybean seed. ß-conglycinin is composed of α’, α and ß subunits, encoded by the genes Cgy1, Cgy2, and Cgy3, respectively. β-conglycinin protein exhibits poor nutritional and food processing properties. Lipoxygenase is responsible for the beany flavor and is responsible for the inferior nutritional quality of unheated or incompletely heated soybean meal. The objective of this research was to select F2 seeds with lipoxygenase-free and low content of ß-conglycinin for breeding of high quality soybean cultivar. A total of 582 F2 seeds were obtained from the cross of 10F1(lipoxygenase-free, normal content of 7S protein) and T311(lipoxygenase-present, low content of 7S protein). Lipoxygenase and 7S proteins in mature seeds were detected by SDS-PAGE. The segregation ratio of 3 : 1 for Shr locus (418 Shr_ : 164 shrshr) were observed. This result shows that the Shr locus is controlled by a single gene. Among 164 shriveled seeds, 6 F2 seeds with lipoxygenase-free, α’ subunit-free, and low content of α and ß subunit were selected. Among 418 normal seeds, 6 F2 seeds with lipoxygenase-free, α’ subunit-free, and low content of α and ß subunit were selected. These results will be used in breeding program for improving high quality soybean cultivar.

We improved the separation of the basic proteins from the soybean cotyledon, Glycine max L. Merr. by searching N-terminal sequences data in proteins isolated by two-dimensional electrophoresis (2-DE). After removed Hexane, proteins were extracted from cotyledon with a urea/Triton/2-mercaptoetanol solution. Using this method, the highly reproducible isoelectric focusing (IEF) can formed with polyacrylamide gels with pH 4.0-9.8. The IEF tube gels were used as the first dimension, and proteins were visualized by second-dimensional gel electrophoresis, and identify a number of soybean cotyledon proteins using mass spectrometry in the proteome analysis. These instruments of 2-DE and IEF tube gels were used 27 cm and investigate under various conditions. The total number of spots and features was obtained by PDQuest software (Bio-Rad). In this experiments performed, the IEF tube gels and instruments afforded good reproducibility in the number of PDQuest-detected spots from gel to gel while IPG offered better reproducibility in the total number of manually detected spots from gel to gel. In conclusion, we have separated of the basic 13 proteins in soybean. The glycinin subunit separations are also considered to play important roles in soybean breeding and biochemical characterization. The improved technique will be useful to dissect the genetic control of glycinin expression in soybean.

Two hundred wild soybean lines were used to investigate water imbibition of seeds and its relationships with seed coat structure. Those structures were observed under the light microscope and the surface structure and cell arrangement was done under the scanning electron microscope. One hundred and ninety seven out of 200 wild soybean genotypes (98.5%) had hard seed and only three wild soybean genotypes, YWS16, YWS67, and YWS136 collected in Korea showed the permeability to water. Those three permeable lines showed less endocarp deposits whereas the hard seed lines did thick deposits. Larger cracks were found in permeable seed lines, however, no crack or tightly cracks were found in hard seed lines. The structure in the cell arrangement of palisade layer was looser than hard seed lines. The less compact cell arrangement in palisade cell layer and lower endocarp deposits with large cracks were considered as main factors affecting the permeability of wild soybean seeds.

옥수수 우량 교잡종 육성을 위한 자료로 활용하고자 국내 육성 옥수수 자식계통, F1 종실 및 F2 종실의 이화학적 특성과 지방산 및 아미노산 조성을 각각 분석 검토하였다. 1. F2 종실의 단백질 함량이 9.0%로 자식계통 11.4%와 F1 종실 10.9%에 비하여 유의하게 낮은 것으로 나타났다. 지방 함량은 F2 종실이 자식계통과 F1 종실보다 높았다. 회분은 자식계통, F1 종실, F2 종실간 조성변이에 차이가 없었다. 2. 자식계통, F1 종실, F2 종실 모두 linoleic acid의 조성비가 가장 높고 oleic acid, palmitic acid, stearic acid, linolenic acid의 순으로 지방산 조성비가 높은 것으로 나타났다. F2 종실의 포화지방산의 함량은 17.6%로 F1 종실과는 비슷하였으나, 자식계통보다는 낮았다. F2 종실의 불포화지방산의 함량은 82.4%로 F1 종실과는 비슷하였으나, 자식계통보다는 높았다. 3. F2 종실의 leucine의 함량이 자식계통 및 F1 종실보다 낮았다. 반면 F2 종실의 valine, serine, threonine, cysteine, aspartic acid, lysine, arginine, histidine 등의 함량이 자식계통 및 F1 종실보다 높았다. Alanine 등 나머지 아미노산은 자식계통, F1 종실, F2 종실간 조성변이가 비슷한 경향이었다. 4. 산성아미노산인 MMA(monoamino monocarboxylic acid)가 자식계통, F1 종실, F2 종실에서 그 조성 비율이 가장 높았으며 산성 아미노산인 DMA(diamino monocarboxylic acid), 방향족 아미노산인 AAA(aromatic amino acid) 순으로 조성비가 높았다.

바이오에너지 작물로 주목받고 있는 억새(Miscanthus sinensis)의 성숙 종자로부터 캘러스를 유도하고 식물체를 재생시키는 방법을 개발하였다. 억새 종자로부터의 캘러스 유도에 적합한 조건을 알아보고자 기본배지와 2,4-D의 농도, proline의 농도, 배지고형제의 종류와 농도 처리를 각각 실시하였다. 기본배지로 MS배지와 N6배지를 두고 2,4-D를 농도별로 처리하였을 때, MS 배지에 2,4-D 4.0 mg/L를 첨가한 처리구에서 캘러스 형성율이 42.2%로 가장 높았다. Proline은 종자로부터 캘러스 형성을 증가시키는 효과가 있었고, 3.0 g/L 이하의 저농도에 비해 12.0 g/L를 첨가하는 것이 캘러스 형성에 좋았다. 배지 고형제는 agar에 비해 Phytagel이 효과적이었으며 Phytagel 2.25 g/L를 첨가한 처리구에서 캘러스 형성율이 86.1%로 가장 높았다. 억새의 종자로부터 유도된 캘러스는 4주 간격으로 계대배양하였고, 캘러스 유도 3개월 후 진한 노란색의 둥근 돔 형태를 갖는 캘러스를 분리하여 신초 재생에 이용하였다. 종자로부터 유도된 캘러스를 호르몬 무첨가 MS 배지 또는 MS 배지에 BA 1.0 mg/L를 첨가한 배지에서 배양하여 신초 재생을 유도할 수 있었다. 재생된 신초는 신장과 발근을 거쳐 소식물체로 발달하였으며, 소식물체는 성공적으로 활착되었고 정상적인 표현형을 나타냈다.

This paper describes the potential use of deep sea water to stimulate seed germination in both common and Tartary buckwheat. Treatment of 10% deep sea water at 25℃ would slightly enhance germination of buckwheat seeds compared to non-DSW treatment and other temperature. In this study, the significant effects of photoperiod and temperature on seedling growth were also found in the HL treatment for the number of leaf, plant height, and plant fresh weight and LL treatment for root length and leaf size. Common buckwheat (Suwon No.1) showed higher rate (93%) of flowering plants in the HS and LL (93% of flowering rates) than those revealed in the HS and LS treatment, while the low percentage(67%) of plant flowering plants was shown in the LS treatment. All plants (100%) of a Korean landrace, Ahndong-jaerae showed flowers in the HS and LS treatment. HL and LL treatment status did not occur in the plant's flowering. Any Tartary buckwheat (KW45) plant did not yet flowered when it was 21 days-old.

This study is part of research to develop the technology for managing major medicinal crops after harvest. We studied the optimal germination conditions of Cynanchum wilfordii (Maxim.) Hemsl. The mean germination time (MGT) of C. wilfordii seeds was higher after soaking for 4 days after storage at 5℃ for 8 weeks, than with germination at 25℃ (3 days). However, the germinative energy (GE) decreased as the number of days soaking increased. The greatest germination rate (83.3±8.8%) was at 25℃ with no soaking of seeds stored at -20℃ for 8 weeks. Based on these results, we characterized the germination conditions of a major medicinal crop.

The objective of this study was to rapidly evaluate fatty acids in a collection of millet (Panicum miliaceum subsp. miliaceum) of different origins so that this information could be disseminated to breeders to advance germplasm use and breeding. To develop the calibration equations for rapid and nondestructive evaluation of fatty acid content, near-infrared reflectance spectroscopy (NIRs) spectra (1104-2494 nm) of samples ground into flour (n =100) were obtained using a dispersive spectrometer. A modified partial least-squares model was developed to predict each component. For foxtail millet germplasm, our models returned coefficients of determination (R2 ) of 0.89, 0.89, 0.89, and 0.92 for palmitic acid, oleic acid, linoleic acid, and total fatty acids, respectively. The prediction of the external validation set (n=10) showed significant correlation between references values and NIRs values (r2 =0.64, 0.90, 0.79, and 0.89 for palmitic acid, oleic acid, linoleic acid, and total fatty acids, respectively). Standard deviation/standard errors of cross-validation (SD/SECV) values were close to 3 (2.62, 2.40, 1.85, and 2.23 for palmitic acid, oleic acid, linoleic acid, and total fatty acids, respectively). These results indicate that these NIRs equations are functional for the mass screening and rapid quantification of the oleic and total fatty acids characterizing millet germplasm. Among the samples, IT153514 showed an especially high content of fatty acids (48.14mg~;g-1 ), whereas IT123909 had a very low content (34.44mg~;g-1 ).