Linear and nonlinear models are available to simulate the effect of mine blasting. And the results are changed by selection of each model. Plaxis 8.0, FLAC 4.0 are used in this study for simulating blasting pressure. These programs offer linear model and nonlinear model. Linear model can properly simulate loss of impact wave in plastic failure region based on comparison of site vibration measurement and numerical analysis iteratively.

Sorghum seed is traditionally used as secondary food sources in addition to rice in Korea. While the hypoglycemia regulating phytochemicals have been found in sorghum seed, peptides related with hypoglycemia never been studied before. To obtain the peptide characteristics and the specifically high-expressed peptides in hypoglycemic sorghum seed, peptide profiles of seven hypoglycemic and five nonhypoglycemic sorghum lines bred in RDA were determined using surface-enhanced laser desorption/ionization time-offlight mass spectrometry (SELDI-TOF MS). The twelve sorghum lines exhibited 104 peptides on CM10 protein chip array (weak cation exchange) and 95 peptides on Q10 (weak cation exchange) in the molecular mass range from 2,000 to 20,000 Da. Heat map via supervised hierarchical clustering of the significantly different peptides (p < 0.01) in peak intensity among the 12 lines effectively revealed the specifically upregulated peptides in each line and distinguished between 7 hypoglycemic and 5 non-hypoglycemic lines. Through the comparison with hypoglycemic and non-hypoglycemic lines, 10 peptides including 2231.6, 2845.4, 2907.9, 3063.5, 3132.6, 3520.8, 4078.8, 5066.2, 5296.5, 5375.5 Da were specifically high-expressed in hypoglycemic lines at p < 0.00001. This study characterized seed peptides of 12 sorghums and found ten peptides highly expressed for hypoglycemic sorghum lines, which could be used as peptide biomarkers for identification of hypoglycemic sorghum.

지금까지 밥맛특성이 우수한 품종을 육성하려는 노력이 부단히 진행되고 있으나, 저세대에서 미질을 평가하는 기준과 지표가 미흡한 실정이다. 고식미 품종육성과정 중 저세대에서 고식미계통을 선발하기에는 어려운 실정이다. 본 연구에서는 DNA분석에 의한 식미회귀식값을 자포니카 간의 교잡에서 유래된 저세대 계통선발에 적용하여 선발효율성을 관행육종법과 비교분석하였다. 9개 교배모본들을 군집분석하여 그룹간에 식미회귀식값과 밥의 윤기치의 연관분석을 실시한 결과, 유의성이 있었다. 분자육종법과 관행육종법으로 계통을 선발한 결과 선발집단규모는 각각 34.4%, 38.6%로 비슷하였고, 분자육종법과 관행육종법을 병행하였을 때는 19.5%로 집단규모가 상당히 줄었다. 밥의 윤기치와 식미회귀식값은 집단간에는 차이가 있었으나, 육종방법에 따라서는 차이가 없었다. 식미회귀식값은 13개 DNA마커를 가지고 값을 구하게 되는데, 본 실험에 사용된 교배조합에서 양친 간에 다형성을 보이는 DNA마커는 3～7개로 상당히 적어, 식미회귀식값을 구하여 표현형을 설명하기에는 부족한 것으로 생각된다. 식미회귀식값에 의한 고식미계통선발을 하려면 교배조합별로 DNA마커조합과 식미회귀식값을 다시 산출해서 사용을 하거나, 식미회귀식에 활용되는 모든 DNA마커에 다형성을 보이는 교배조합을 선정해서 적용한다면 어느 정도 효과가 있을 것으로 생각된다. 본 실험에 활용된 식미연관 13개 DNA마커에 의한 식미회귀식값은 모든 조합의 육종집단에서 선발에 활용하기는 어려울 것으로 생각된다.

A number of problems associated with environmental noises in urban areas have significantly been considered. Specific measurement and estimation of the environmental noise became a primary issue in local governments. Environmental noise monitoring system is required in order to estimate and verify the a city noise map. However, current monitoring positions may not perfectly represent and incorporate many different view points, such as districts of a city, different utilizations of a city by the law, populations, and classifications and traffics of roads. In addition, scientific method to provide specific noise monitoring positions my not be avaliable in current literature. For this reason, the primary objective of this paper is to propose a new method for introducing a number of monitoring positions in the entire city. First, the quality function deployment (QFD) method was utilized to simultaneously represent both districts and utilizations of a city. Second, a new algorithm to find a number of monitoring positions was proposed by compromising many different view points: populations, classifications of roads and areas, and traffics of roads. Finally, the proposed monitoring positions and a sample noise map was provided for verification purposes. Based on these results, the proposed algorithm including the QFD concept may successfully provide specific noise monitoring positions by simultaneously consider may different view points and requirements of a city.

Genome polyploidization has provided significant sources of genetic variation for plant adaptive evolution and new species formation. However, the way in which molecular evolution of polyploid genomes builds up genetic architecture underlying speciation is unclear and whether there are any differences in polyploidsubgenomes’s responses to selection is unknown. Brassica is an ideal model to address these questions. Here, we used Arabidopsis thaliana as an outgroup to conduct comparative genome analysis of newly sequenced Brassica oleracea, B. rapaand B. napus. We revealed multi-layered modes of asymmetrical interspecific and intraspecific genome evolution. Between parallel species B. oleracea and B. rapa, these layers include: asymmetrical gene retention rates,asymmetrical TE amplification, asymmetrical tandem duplication of genes and asymmetrically alternative splicing variantsbetween the two sister species; Between subgenomes within species, these layers include: massive and asymmetrical subgenomic gene loss, great variations between paralogs at the DNA sequence level, expression differentiation of triplicated, α-duplicated and tandem duplicated genes across different tissues in the two diploid species, asymmetrical recombination on A and C in B. napus. In addition, the predominant mechanism for gene loss is small deletion, rather than asymmetrical cross-over. These patterns provide new insight into genome evolution underlying speciation and trait formation and will underpin research into genetic improvement of these important crops

Green rice leafhopper(GRH), Nephotettix cincticeps Uhier, is one of the major insect pests of rice (Oryza sativa L.) in the temperate growing region of East Asia. GRH sucks sap from both xylem and phoem of susceptible rice varieties, and increased GRH populations cause sooty mold disease on the ears of rice after heading stage. In addition to direct plant destruction, GRH also causes damage to rice plants by transmitting rice dwarf viruses causing rice dwarf viruses disease which could decrease the yield of rice. Development of GRH resistant rice varieties for reducing yield loss is an important objective in current breeding programs. In this study, we developed three SSR markers(RM18166, RM516, RM18171) and one Indel marker(Indel15040) which could select Grh1-resistant varieties using population derived from cross lines between Grh1-resistant variety ‘Singwang’ which contains Grh1 gene and susceptible variety ‘Ilpum’. PCR products of RM18166 which was one of the developed markers were easily detected in agarose gel. These markers will be useful for development of the Grh1-resistant varieties through marker-assisted selection(MAS) without bio-examination in rice breeding

Bt gene derived from the B. thuringiensis has been used for developing GM crops, and corn, cotton and soybean producing B. thuringiensis toxins have been on the market for last 17 years or so creating a huge GM seed industry. One of the notorious pests in brassica crops is diamondback moth (DBM). In order to protect the insect plague of crops from DBM, 4-5 billion dollars have been wasted annually for applying integrated measures in worldwide. Major prevention is use of pesticides that may build the contamination level of chemicals in the ground and this practice threats the environment and ecosystem. An alternative is to develop GM brassica crops and therefore we have developed GM cabbages resistant DBM using bt gene. Lots of T0 cabbages were tested for resistance and independent GM cabbages resistant to DBM were selected. Molecular analysis was conducted to find if GM cabbage holds one copy transgene and intergenic insertion. We found an independent GM cabbage and it contained a singly copy of the transgene without disturbing the insertion site. This one called C95 line with an status of event have been self-crossed for two generation (T2). Also we are working the development of GM cabbage with different vector that contains bar gene as a selection marker. So far 17 T0 cabbages have been obtained by bar selection.

Many viruses infect cucurbits. One of the well-known symptoms is mosaic disease. Those that cause mosaic are cucumber mosaic virus (CMV), squash mosaic virus (SqMV), watermelon mosaic virus (WMV), zucchini yellow mosaic virus (ZYMV) and cucumber green mottle mosaic virus (CGMMV). WMV resistant GM squash was developed many years ago in the United States and it was on the market, but no further information was available by now pertinent to commercial aspect. Usually these viruses are not easily controlled by frequent applications of chemicals that target the insect as carriers of viruses. Therefore, it is necessary to develop commercial varieties possessing resistance against viral diseases. We have developed GM watermelon rootstocks called gongdae, using a coat protein gene of CGMMV as transgene. Those GM watermelon rootstocks showed highly resistant to CGMMV, and have been crossed to get the several BC and T generation. In order to obtain the virus resistant watermelon, watermelon lines were crossed to the selected GM watermelon rootstock. Here, we present the successful watermelon cultivars that show resistance to CGMMV. The resistance must have obtained by transferring the transgene from the GM watermelon rootstock to watermelon line

Two carotenoid biosynthetic genes, phytoene synthase (Psy) and carotene desaturase (CrtI) linked via synthetic 2A sequence under control of CaMV 35S promoter (two T0 plants 5 and 6) or β- conglycinin promoter (three T0 plants 7, 13 and 16) were transformed into soybean variety Kwangan. After agronomic and phenotypic selection at early generations, T5 progeny of PAC soybean were analyzed by Southern blot to confirm T-DNA copy numbers. A total of 27 homologous lines derived from one of three T0 plants (line 7 under the control of β- conglycinin promoter) with one copy T-DNA insertion, were separated and planted into greenhouse. Flanking sequence analysis was carried out on one of homologous line 6-2-3 and results indicated the T-DNA was intergenic inserted into chromosome 14 from 10,873,131 to 10,872,998 base of soybean chromosome. T-DNA insertion structure, flanking sequence and inserted gene expressions need to be analyzed in the further study.

In this study, the concentrations of isoflavones, anthocyanins and total phenol content (TPC) in 19 soybean mutant lines changed seed coat color from yellow to black or brown were determined. Among 19 soybean mutant lines, 5 soybean mutant lines with black pigment were detected 3 anthocyanins (delphinidin-3-O-β-D-glucoside, D3G; cyaniding 3-O-β -D-glucoside, C3G; petunidin 3-O-β-D-glucoside, Pt3G). The highest concentration of anthocyanins among 5 soybean mutant lines was D-16 (1280.0 ± 19.4 mg/100g seed coat) derived from cv. Danbaek. Although isoflavone contents of all soybean mutant lines showed lower levels compared to original cultivars, glycitein was detected only 5 soybean mutant lines (DP-37-2, DP-38, DP-39, DP-40, and DP-41 derived from cv. Daepung). In TPC of 19 soybean mutant lines, DP-10 was increase levels compared to original cultivar, while DP-37-2, DP-40, and DP-41 were decrease levels of TPC. Using reduction of DPPH, we measured the free radical scavenging activity (FRSA) among 19 soybean mutant lines. Five black and 4 brown soybean mutants showed significant increase in FRSA. On the basis of these results, it was concluded that gamma irradiation may change the isoflavone, anthocyanin, and total phenol contents of soybean. These mutant lines using in this study can be useful for the breeding of soybean varieties altering the nutritional values.

With the economy development, people have an increasing demand for meat and eggs. Hence the market demand for cereal crop keeps increasing. maize is the major crop to meet the conditions of market demand. As a part of the Golden Seed Project, we are planning to develop maize varieties for adaptation in Southern Asia, especially in India. This project was started at 2013 and has been collaborated with Nongwoo Bio Co. Our project is based on overseas maize breeding in Bangalore, India. At first year, we tested 40 maize hybrids to investigate adaptability, yield capacity, and growing characteristics. Planting materials for this test were developed and selected in Cambodia by researchers of Maize Research Institute. Growing performance is usually quite different by the growing area and seasons, but some of our materials have shown good adaptability and yield at dry season in Southern India. 13IN07 was selected by yield and grain color, 13IN39 was selected by yield and plant type. These two selected hybrids were applied to regional test controled by Indian Council of Agricultural Research in India. We will see how our hybrids adapt in various regions in India, assess how other tested hybrids perform in those regions, and compare each other. We may also expect one of the selected hybrids can be a candidate variety for some regions in India and we will continuously select good hybrids and develop new varieties in India.

A total area of reclaimed land in Korea is about 135,100 ha, which occupies 9 % of total arable land. Soybean is one of the most important crop in Korea and demand for the crop is increasing, while the country’s self-sufficiency is very low, around 9 %. If it’s possible to cultivate soybean in reclaimed land, it would increase self-sufficiency of the soybean. However, there are difficulties to cultivate soybean in reclaimed land because of excessive level of salinity in the soil, to prevent this barrier in saline soils, it is necessary to develop salt tolerant soybean cultivar. This research was conducted to select salt tolerant lines derived from PI 483463 (salt tolerant wild soybean accession). The F1 (Hutcheson × PI 483463) was backcrossed with Hutcheson and Wooram (salt sensitive soybean cultivar). For marker assisted selection and salt reaction phenotyping, randomly selected BC1F1 seeds from two backcross populations were planted in 11 cm tall tray. At the V1 growth stage, DNA was sampled with FTA card. The genomic DNA and SSR marker, BARCSOYSSR_03_1348, were used for PCR amplification and the result was checked through electrophoresis. The trays with BC1F1 plants were immersed in 100 mM NaCl solution up to the bottom third of the trays directly after the DNA extraction. After two weeks, phenotype was measured depending on leaf scorch degree. Through this research, 25 dominant homozygote lines and 22 heterozygote lines from Hutcheson backcross population and 28 dominant homozygote lines and 37 heterozygote lines from Wooram backcross population were selected. These lines will be used for developing soybean with salt tolerance

Soil salinity limits crop productivity in many regions. This problem would be more serious as the global climate changes and worldwide water shortages would accelerate soil salinization. This study is fulfilled with aim on resolve crop cultivation in dry/saline land as an international joint research project with Tunisia. Total 48 lines of wheat cultivars including 32 common wheat (16 Korean wheat, 16 Tunisian common wheat) and 16 Tunisian durum wheat were incorporated in this study. Salt stress was applied for 2 weeks by submerging the pots into 500 mM NaCl at 3-leaf stage followed by re-watering for restoration in greenhouse. Numerous agronomic/growth parameters were scored for tolerence. SSR primers that have been known to be related to salt tolerance were applied to explain selected population. The correlation between PCR-based length polymorphism of selected lines and their resistance were evaluated. The obtained information will aid selection for salt tolerance hexa/tetraploid wheats. Acknowledgement: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 2012K1A3A1A09028123) and carried out with the support of “Cooperative Research Program for Agriculture Science & Technology Development (Project title: Development of high yielding wheat with stress tolerance via molecular breeding strategies, Project No. PJ008031)”, Rural Development Administration, Republic of Korea.

The portfolio selection is one of the most important and vital decisions that a real or legal person, who invests in stock market, should make. The main purpose of this article is the determination of the optimal portfolio with regard to relations among stock returns of companies which are active in Tehran’s stock market. For achieving this goal, weekly statistics of company’s stocks since Farvardin 1389 until Esfand 1390, has been used. For analyzing statistics and information and examination of stocks of companies which has change in returns, factors analysis approach and clustering analysis has been used (FC approach). With using multivariate analysis and with the aim of reducing the unsystematic risk, a financial portfoliois formed. At last but not least, results of choosing the optimal portfolio rather than randomly choosing a portfolio are given.

Localization is one of the essential tasks necessary to achieve autonomous navigation of a mobile robot. One such localization technique, Monte Carlo Localization (MCL) is often applied to a digital surface model. However, there are differences between range data from laser rangefinders and the data predicted using a map. In this study, commonly observed from air and ground (COAG) features and candidate selection based on the shape of sensor data are incorporated to improve localization accuracy. COAG features are used to classify points consistent with both the range sensor data and the predicted data, and the sample candidates are classified according to their shape constructed from sensor data. Comparisons of local tracking and global localization accuracy show the improved accuracy of the proposed method over conventional methods.

정부는 2010년 신재생에너지발전전략을 수립·시행함에 따라 2011년 11월 “서남해안 2.5GW 해상풍력개발 종합추진계획”을 발표하 였다. 이와 같이 친환경에너지 정책에 따라 해상에 설치되는 풍력발전단지는 계속 증가할 것이다. 해양풍력발전단지의 개발은 풍력뿐만 아니 라 해상교통환경도 고려해야 한다. 특히 풍력발전단지의 항로표지는 인근지역을 항해하는 선박과 구조물의 충돌을 방지하는 중요한 역할을 한다. 국제항로표지협회에서는 풍력단지의 식별을 위해 해양풍력단지의 가장자리 구조물(SPS)과 외각 선의 중간 구조물(IPS)에 등광을 설치 하도록 규정하고 있다. 그러나 이들 등광을 식별함에 있어 중요한 역할을 하는 등질에 관해서는 언급하지 않고 있고 적합한 등질 선택에 관한 연구도 부족한 실정이다. 따라서 본 연구에서는 해양풍력발전단지를 표지하는 SPS와 IPS의 등광설치에 관한 국내외 규정을 검토하고 다른 항로표지 등광과 혼동되지 않으면서 식별이 용이한 등질의 패턴과 리듬을 제안한다. 제안된 등질은 시뮬레이션 검증을 수행하였으며, 그 결과 SPS의 등질 “Fl Y(4) 12s”와 IPS의 등질 “Fl Y 6s”의 조합 동기점멸이 유용한 것으로 분석되었다.

Underground material and to secure any other appropriate waterproofing method for improving the workability experiments on non-woven fabric weight is selected. As a result, the appropriate non-woven fabric weight of 140 g/㎡ was confirmed.

This research analyzed the effects of satisfaction factors of rural experience of city dwellers. The main research results are summarized as follows. The selection attributes for the rural experience of urbanites were divided into their interests into the programs, variety of the programs and convenience of the facilities. According to the multiple regression analysis, interests into the programs had impacts on the level of satisfaction. The types of rural tourism had statistical relevance with people’s interests into the programs, convenience of the facilities and variety of the programs. In addition, those at the age of 40 to 50 reported higher satisfaction in their interests of the programs and convenience of the facilities among the general attributes of the respondents. In occupation, office workers had higher satisfaction in their interests into the programs while homemakers were more satisfied than others in convenience of the facilities and variety of the programs. When it comes to the monthly family income, those in the group who earn 2 to 3 million won a month had higher satisfaction in terms of convenience of the facilities and variety of the programs.