Anaerobic germination (AG) is plays important role in submergence resistance which is an important trait for rice production in flood-prone lowland areas. Slow seed germination and delayed seedling establishment due to flooding become major problem for modern sowing methods such as direct seeding and environmental friendly good agricultural practices especially using young seedling age. In total, 137 diverse rice accessions were evaluated for anerobic germination ability. Submergence 1 (SUB1) which is induced ethylene response factors is suggestive because genes belonging to this gene family play a crucial role in rice tolerance to submergence. In this study, haplotype variations of three AG related genes, SUB1 (SUB1A, SUB1B, SUB1C) were examined using whole-genome resequencing data of 137 accessions of rice core set. The new SNPs and InDels found in the exon of the sub1 loci would be useful in developing markers to screen the varieties with strong anaerobic germination ability in the future molecular breeding

Salt toxicity is the major factor limiting crop productivity in saline soils. Rice is an important staple food crop of nearly half of the world population and is well known to be a salt sensitive crop. The completion and enhanced annotations of rice genome sequence has provided the opportunity to study functional genomics of rice. With the rapid development of the biotechnology techniques, we can use more accurate and reliable methods to study the mechanism and function in different stress conditions. In present study, 295 rice accessions of diverse origin were re-sequenced and used for genome-wide association study (GWAS) with several germination-related traits, including germination percentage (GP), germination energy (GE), germination rate (GR), germination index (GI), salt tolerance index (STI) in salt tolerant germination stage. Phenotyping of the rice accessions were carried out at 200mM NaCl to screen salt tolerance levels. GWAS was applied to detect the associated genes related to salt tolerance in rice germination stage. Variations and haplotypes of the associated genes were detected and correlation between the phenotypes and genotypes were validated using qRT-PCR.

Glucosinolates of Brassica rapa collection from Korea genebank were measured to determine total glucosinolate content and their variation of diverse glucosinolates; Around 100 accessions representing the different morphotypes and geographical origin of Brassica rapa were analysed. The principal component analysis was performed to evaluate the differences among morphotypes using the profiles of 14 glucosinolates identified from the leaves. DMRT test and box plots showed the significant difference between total glucosinolates of subspecies. Most of turnip accessions had higher gluconilates compared to the other type accessions, Chinese cabbage and pak choi. These accessions will be used for GWAS study for glucosinolate. Now they are being finger-printed by genotyping by sequencing (GBS). Among these accession, we selected a turnip accession with high amount of glucosinolate, K0466 and two Chinese cabbage accession with low amount of glucosinolate, K0015 and K0621. To analyse quantitative traits loci (QTL) for glucosinolate synthesis, these three accessions were fixed through microspore culture. Finally, six homozygous lines were selected and were crossed each other to make F1 hybrids. We just harvested F2 seeds and transferred doubled haploid plants to pots. QTL analysis for glucosinolate will be performed these F2 and DH population.

This study is to raise the utilization of genetic resource of wheat (Triticum aestivum) landrace by evaluating genetic variation related to end use quality of the Far East. Allelic composition of HMW-glutenin subunits encoded by genes of Glu-1 loci associated with bread baking quality was investigated in 324 wheat landrace genetic resources originated from Korea, China, and Japan. The most frequent combination of HMW-glutenin subunits were Glu-A1c, Glu-B1b in Korean and Japanese resources, but Glu-A1a and Glu-B1c were the most in Chinese resources. By using the Glu-1 score system, 24 accessions were evaluated as 10 out of 10. As for genetic diversity, represented by polymorphic information content(PIC) index, the level of variation of Korean landrace(0.245) was lower than that in China(0.569) and Japan(0.294). When it comes to unique composition, Glu-B1f(13+16) and Glu-D1f(2+10) subunits are only in Chinese resources. Glu-B1d(6+8), Glu-B1e(20), Glu-D1b(7+8), and Glu-D1c (7+9) subunits are only in Korean resources. Consequently, this study showed that Chinese landrace collection was the most highly diverse and had distinctive characteristics compared to Korean and Japanese one. Especially, some resources having preferable genetic stock or high Glu-1 score can be valuable for wheat breeding program.

Rice (Oryza sativa L.) is important crop as a staple carbohydrate sources and was regarded as domesticated in Asia region. The seed dormancy is one of the domesticated traits, and this character allows that plants survive in various environment. The degree of dormancy have been targeted for controling in breeding program while weak dormancy cause pre-harvest sprouting contrary to the nonuniform seed germination by strong dormancy. In this study, we surveyed the variation of germiability in diverse rice genetic resources including japonica and indica ecotype. Overall, the degree of seed dormancy of rice germplasm was distinguished into four groups and admixed types; first group(G-1) included accessions revealing high germiability, the accessions of second group(G-2) acquired the high germiability by after-ripening process, third group(G-3)’s accessions showed high germiability after dormancy breaking process and the accessions of last group(G-4) maintained the low germiability in spite of the dormancy breaking process. Among the 51 japonica accessions G-1, G-2, G-3 and G-4 included 15, 15, 11 and 10 accessions, respectively, and among 40 indica accessions, G-1, G-2, G-3 and G-4 groups included 15, 15, 9 and 1 accessions, respectively. Based on these primary test, we plan to detail examine the seed germiability and survey the genetic factors affecting the degree of germiability in representative accessions of each group

Eggplant (Solanum melongena L.) is an excellent source of vitamin A and C as well as flavonoid compounds, which are important antioxidant components that may reduce the risk of diseases. In this study, we investigated ABTS, DPPH activity and flavonoid contents in eggplant leaves and fruits to identify genetic resources with high antioxidant capacity for use in food or as feed additives. A total of 102 eggplant accessions were classified into four groups by latitude of their origins: 0°~15° N (8 accessions), 15°~30° N (19 accessions), 30°~45° N (34 accessions), and 45°~60° N (41 accessions). The accessions originated from 45°~60°N showed the highest flavonoid contents (AVG. = 15.4 μg mg-1) followed by accessions originated from 30°~45° N (AVG. = 13.0 μg mg-1), 15°~30°N (AVG. = 11.0 μg mg-1) and 0°~15°N (AVG. = 9.5 μg mg-1). Same pattern was also found in ABTS and DPPH antioxidant activities. High ABTS, DPPH activity and flavonoid contents were found in the early-flowering accessions. All flavonoids of the greenish violet leaves were significantly higher than those in green leaves. The flavonoid concentration in eggplant leaves with an average of 15.6 μg mg-1 increased from 10- to 20-fold as compared with their fruit (AVG.=0.9 μg mg-1). In conclusion, eggplant leaves represent a potential source of natural antioxidants due to their very high flavonoid contents.

In southern region of Korea, double cropping system including soybean as a second crop is widely adopted because prolonged high temperature in October enables soybean maturation even in late autumn. Global warming during decades derives transition of soybean planting dates as late as late-July. Delayed planting beyond the critical time moves reproductive stage to less favorable weather conditions like low temperature and short photoperiod, which can affect soybean yield and seed composition. Therefore selection of appropriate cultivar for changed climate and cultural practice is required. Effects of different eco-type and planting date on soybean yield was investigated for three sowing dates of 20 June, 5 July and 20 July with ten cultivars. Strong interaction between cultivar and sowing date was detected in number of branch, node and pod. No significant interaction was observed in 100-grain weight and yield, and this might be resulted from similar yield reduction pattern. Daepung, Taekwang and Uram recorded the highest yield when planted late, proving that these three cultivars are the most adaptable to delayed planting. Delayed planting results in higher sugar content, especially stachyose and raffinose which have detrimental effect on human health by causing flatulence and diarrhea. Protein content was decreased and oil content was increased in July 20 planting. Decrease in protein content was the largest in Hwangeumol and maintained high in Saedanbaek. This information will provide a guideline when select appropriate soybean cultivar in delayed planting to maintain yield and seed quality.

Heading date and photoperiod sensitivity are fundamental traits that determine rice adaptation to a wide range of geographic environments. By quantitative trait locus (QTL) mapping and candidate gene analysis using wholegenome re-sequencing, we found that Oryza sativa Pseudo-Response Regulator37 (OsPRR37; hereafter PRR37) is responsible for the Early heading7-2 (EH7-2)/Heading date2 (Hd2) QTL which was identified from a cross of late-heading rice ‘Milyang23 (M23)’ and early-heading rice ‘H143’. H143 contains a missense mutation of an invariantly conserved amino acid in the CCT (CONSTANS, CO-like, and TOC1) domain of PRR37 protein. In the world rice collection, different types of nonfunctional PRR37 alleles were found in many European and Asian rice cultivars. Notably, the japonica varieties harboring nonfunctional alleles of both Ghd7/Hd4 and PRR37/Hd2 flower extremely early under natural long-day conditions, and are adapted to the northernmost regions of rice cultivation, up to 53° N latitude. Genetic analysis revealed that the effects of PRR37 and Ghd7 alleles on heading date are additive, and PRR37 down-regulates Hd3a expression to suppress flowering under long-day conditions. Our results demonstrate that natural variations in PRR37/Hd2 and Ghd7/Hd4 have contributed to the expansion of rice cultivation to temperate and cooler regions

Most eukaryotic organisms, including plants, display specialized cellular and behavioral rhythms with a period of approximately 24 hours. The circadian clock generates this rhythm to convey daily or seasonal basis of temporal information, coordinating the proper phasing of many important cellular processes. Temperature compensation (TC) is one of the defining features of the clock mechanism. Under this function, the speed of the clock or period remains relatively constant over physiologically relevant temperatures, unlike the biochemical reactions. Thus, TC allows organisms to sustain their life ordinarily in various thermal environments by providing an accurate measure of the passage of time regardless of surrounding temperatures. Previously, Edward and his colleagues performed a quantitative trait loci (QTL) study to find TC related natural variations in the recombinant inbred line (RIL) population from two Arabidopsis ecotypes, which are adapted to different thermal environments; one parent is Cvi accession (Cvi) which originates from the warm climate, Cape Verde Island, and the other is Ler accession (Ler) from Northern Europe. For the two most significant QTLs, the core clock components in Arabidopsis clock, GIGANTEA (GI) and ZEITLUPE (ZTL) are proposed as strong candidates. Moreover, the amino acid substitution leading to GICvi and ZTLCvi (Ler to Cvi) are suggested to be the causal factors for the TC QTLs respectively. However, precise molecular mechanisms of these natural variations on TC are still not understood well. Here, we elucidate the molecular impact of the natural variation shaping GICvi and ZTLCvi on TC function. GICvi and ZTLCvi post-translationally regulate ZTL stability in antagonistic way, resulting in the opposite period/clock effects mediated by ZTL protein abundance. However, if both GICvi and ZTLCvi are present, they mutually balance their own effect on ZTL, which in turn supports TC capacity of Cvi especially at high temperatures. Considering the amino acid residues in GI and ZTL, where the natural variations arise, are highly conserved across many important crop species including rice, corn, cabbage and etc., this research will give valuable insights into the TC related thermal adaptive processes in Arabidopsis as well as those important crop plants.

본 연구는 참깨 유전자원 핵심집단과 주요 품종 및 우량계통의 세사민과 세사몰린, 지방 함량변이를 분석하여 리그난 함량이 높은 참깨 품종육성을 위한 기초자료로 활용하고자 하였다. 본 시험에 이용된 참깨 유전자원 143점의 리그난 함량 범위는 2.33 mg/g에서 12.17 mg/g으로 나타났다. 그 중 터키에서 수집된 IT184615은 리그난 함량이 12.17 mg/g으로 가장 높아 리그난 함량 개량을 위한 유전자원으로 활용성이 높은 것으로 판단되었다. 참깨 유전자원을 14개의 수집원산지별, 4개의 종피색(백, 황, 갈, 흑)별, 4개의 SSR마커그룹으로 분류하고 리그난 함량과의 유의성을 검정한 결과 세사민, 세사몰린 함량은 수집원산지, 종피색 간에 유의한 차가 인정되었다. 러시아(10.0 mg/g), 네팔(9.08 mg/g)에서 수집된 유전자원은 다른 국가에서 수집된 유전자원보다 평균 리그난 함량이 높았다. 세사민 함량은 세사몰린 함량과 정의 상관관계가 있어 리그난 함량이 높은 참깨 품종 육성을 위해서는 두 성분을 동시에 높이는 방향으로 선발되어야 할 것으로 판단된다. 참깨 주요 품종과 우량계통의 리그난, 지방 함량의 지역간, 연차간 변이를 분석하여 품질특성에 미치는 환경의 영향을 분석한 결과 리그난과 지방 함량은 지역간, 연차간에 고도로 유의적인 차이가 인정되었고 지역간 변이가 계통 간 변이보다 커서 유전변이보다 환경변이의 변이의 폭이 넓었다. 따라서 리그난, 지방 함량이 높은 참깨 품종 육성을 위해서는 함량이 높은 유전자원을 육종재료로 활용함과 동시에 리그난 축적에 관계되는 재배환경에 관한 연구가 지속적으로 이루어져야 할 것이다.

Seasonal and stational variation of SS and COD were investigated from February 2008 to December 2010 and the relationship between them was discussed. During three years monitoring, SS decreased significantly (46% decline) possibly due to the increase of precipitation and thereby resulting salinity drop. COD on average was the highest in 2009. SS was the highest in autumn and the lowest in winter, and over 72% of SS was FSS. While SS is high in the upper sampling stations of the bay with shallow water, COD values do not show any relationship to the geomorphological characteristics. CODins, which was defined as COD after filtration, ranged 56%(winter) ~ 44.6%(summer) and showed no correlation with SS. It indicates that high SS concentration is not necessarily related to the high CODins. The seasonal CODins/SS data, which can be interpreted as COD density in SS, shows that SS in winter contains the dense COD materials compared to the other seasons.

The exposed population of a vulnerable group to high ozone episodes (exceeding 60 ppb/8h) was estimated in Busan metropolitan city from 2000 to 2010. The frequency of high ozone days at monitoring sites and the number of the population aged over 65 were used to calculate the accumulated (total, seasonal, and yearly) number of the exposed older population (EOP) to high ozone episodes during the study period based on administrative areas, by interpolation and zonal mean methods in ArcGIS software. The older population in this city had increased significantly from 2000 to 2010 (representing over 10% of the total population in 2010). The vulnerable areas (e.g. the eastern area of the city) of the EOP to high ozone episodes were different from the areas with frequent high ozone episodes (e.g., the western area) due to the increase of the older population in particular areas. The difference was more significant in spring than in any other season, and in 2010 than in previous years (2000 and 2005).

Temporal variation of groundwater levels in Jeju Island reveals time-delaying and dispersive process of recharge, mainly caused by the hydrogeological feature that thickness of the unsaturated zone is highly variable. Most groundwater flow models have limitations on delineating temporal variation of recharge, although it is a major component of the groundwater flow system. A new mathematical model was developed to generate time series of recharge from precipitation data. The model uses a convolution technique to simulate the time-delaying and dispersive process of recharge. The vertical velocity and the dispersivity are two parameters determining the time series of recharge for a given thickness of the unsaturated zone. The model determines two parameters by correlating the generated recharge time series with measured groundwater levels. The model was applied to observation wells of Jeju Island, and revealed distinctive variations of recharge depending on location of wells. The suggested model demonstrated capability of the convolution method in dealing with recharge undergoing the time-delaying and dispersive process. Therefore, it can be used in many groundwater flow models for generating a time series of recharge.

On this study, a compressive strength with variation of oxide aluminum contents on calcium aluminate cement used for particular purpose due to good properties is analyzed. Thus, the results propose future research direction.

In this study, natural fine aggregates(NFA) and recycled fine aggregates(RFA) was applying in the lightweight foamed concrete. To replacement of OPC, high volume blast furnace slag was used and incineration ash and recycled aggregates powder were used as alrali activator, Fundamental performances of lightweight famed concrete has been tested. Results showed that as the increase of foam conduction ratio, the strength and the density has been decreased

In this study, before and after sunset carbon dioxide concentration and air temperature were observed in two points of atmosphere (lower observation point of the GL + 0.1 m, the upper observation point of GL + 1.0 m) on the foreshore at located in Suncheon Bay and their variations were analyzed. Observation was performed on the foreshore on 2~4 August 2010. Instrument (VAISALA, GMP343) was set two hours before sunset and then observation was made continuously for six hours. In three days, observed carbon dioxide concentration was 375~419 ppm, and the air temperature was in the range of 28.7~32. 5℃. The average concentration of carbon dioxide was 388~399 ppm in the upper observation point and 386~396 ppm in the lower observation point. It was higher in the upper observation point and its fluctuations were similar in two observation points. Correlation coefficients between carbon dioxide concentration and air temperature in the upper observation point were in the range of –0.64~–0.88, and were calculated –0.65 to -0.90 in the lower observation point. For the carbon dioxide concentration, correlation coefficients between the upper part and the lower part were very high as 0.98 in three times. For the air temperature, correlation coefficients between the upper part and the lower part were very high as 0.97 and 0.99. In the same observation time, the slope of the linear regression function as carbon dioxide concentration in the lower observation point for the upper observation point was in the range of 0.97~1.01. Carbon dioxide concentration was slightly higher in the upper observation point. Because carbon dioxide in the lower observation point was closer on the surface of the foreshore and absorbed from atmosphere to the foreshore. In this study, it was showed that the vertical variation of carbon dioxide concentration was insignificant in the several meter scale of atmosphere on the surface of the foreshore.

본 연구는 남부지역에서 주요 콩 품종의 파종기 이동이 isoflavone 등 생리활성물질에 미치는 영향을 구명하고자 전남 나주(위도 35° 04'N, 경도 126° 54'E)에서 2008년부터 2010년까지 3년간 수행되었다. 여름형콩 품종인 새올콩과 다원콩, 가을형콩 품종인 태광콩, 풍산나물콩 및 청자3호를 이용하여 파종기를 5월 15일, 5월 30일, 6월 15일, 6월 30일, 7월 15일에 실시하였으며, 주요 결과를 요약하면 다음과 같다. 1. Daidzein 함량은 모든 시험품종에서 genistein과 total isoflavone 함량은 청자3호를 제외한 시험품종에서 파종이 늦어질수록 증가하는 경향이었다. 반면에 glycitein 함량은 다원콩을 제외한 시험품종에서 파종기간 유의차가 없었다. 2. Cyanidin-3-glucoside(C3G) 함량은 다원콩과 청자3호, delphinidin-3-glucoside(D3G)와 total anthocyanin 함량은 다원콩에서만 파종이 늦어질수록 유의하게 증가하였다. 그러나 pelargonidin-3-glucoside(Pg3G)와 petunidin-3-glucoside(Pt3G) 함량은 파종기간 유의차가 없었다. 3. 청자3호의 lutein 함량은 파종기에 따라 2.7～3.0 μg/g로 유의차가 없었고, chlorophyll 함량은 파종이 늦어짐에 따라 증가하여 6월 30일과 7월 15일 파종에서 유의하게 많았다. 4. 조지방 함량은 태광콩과 청자3호는 조기에 파종할 때 많았고 기타 시험품종은 파종기간 유의차가 없었다. Palmitic acid 조성비는 대부분 시험품종에서 파종기의 영향을 받지 않았다. Stearic acid 조성비는 새올콩, 다원콩, 청자3호에서 파종이 늦을수록 증가하였다. Oleic acid, linoleic acid 조성비는 새올콩에서만 파종기가 빠를수록 유의하게 높았다. Linolenic acid 조성비는 대부분 시험품종에서는 파종이 늦을수록 유의하게 증가하였다. 5. 파종기 이동에 따른 탄수화물과 조단백질 함량은 대부분 시험품종에서 유의차가 없었다. 그러나 새올콩은 조기에 파종할수록, 다원콩은 만기에 파종할수록 조단백질 함량이 많은 경향이었다.

본 연구는 벼의 출수 후 일수에 따라 수발아 양상을 조사하고 수발아 피해가 발생할 수 있는 시기를 예측하여 수발아 방제를 위한 기초자료를 얻고자 수행하였으며, 그 결과를 요약하면 다음과 같다. 1. 벼 등숙기간 중 호품벼는 출수 후 20일, 신동진벼는 출수 후 30일부터 수발아 조건이 충족되면 발아가 시작되었으며, 50% 이상의 종자가 발아력을 갖는 시기는 호품벼가 약 35일, 신동진벼가 약 45일이었고, 출수 후 50일에는 두 품종에서 모두 80% 이상의 종자가 발아하였다. 2. 한 이삭 내에서 종자의 위치에 따른 수발아의 순서는 상위부 > 중위부 > 하위부 순으로 나타났으며 지경별로는 2차 지경보다는 1차 지경의 것이 높게 나타났다. 3. 수발아 검정 시 미발아된 종자는 배의 미숙, 종자휴면, 종자의 등숙불량 (불완전립)이 원인인 것으로 판단되었다. 4. 출수 후 30~45일의 등숙중기에 신동진벼는 호품벼보다 종자 휴면성이 높았다.