The virus-like particle (VLP) is similar to a pathogenic virus and has a high immunogenicity. However, in the selection of various structural proteins to form VLP, all expression systems commonly consume most of the time and suffer from various difficulties. Therefore, there is a need for a system that can rapidly determine the structural proteins required for effective VLP production. This study aims to construct a transient expression platform using insect cells to solve this problem. Plasmid-based expression vectors and baculovirus-inducible expression vectors were constructed. The vectors were evaluated for overexpression using EGFP. We also confirmed the formation of virus like particles through overexpression of virus structural proteins.

본 연구는 농업 및 정보 통신 기술의 융합을 기반으로 국내외 스마트 농장 서비스 모델을 검토하고 한국의 스마트 온실을 개선하기 위해 필요한 다양한 요인을 조사 하기 위해 수행되었다. 국내 스마트 온실의 작물 생육모델 및 환경모델에 관한 연구는 제한적이었고, 연구를 위한 인프라를 구축하는 데는 많은 시간이 필요하다. 이러한 문제의 대안으로 클라우드 기반 연구 플랫폼이 필요하다. 제안된 클라우드 기반 연구 플랫폼은 통합 데이터, 생육 환경모델, 구동기 제어 모델, 스마트 온실 관리, 지식 기반 전문가 시스템 및 농가 대시보드 모듈을 통해 통합적 데이터 저장 및 분석을 위한 연구 인프라를 제공한다. 또한 클라우드 기반 연구 플랫폼은 작물 생육환경, 생산성 및 액추에이터 제어와 같은 다양한 요인들 간의 관계를 정량화하는 기능을 제공하며, 연구자는 빅데이터, 기계 학습 및 인공지능을 활용하여 작물 생육 및 생장 환경 모델을 분석할 수 있다.

More people travel oversea as the economic size of Korea is getting larger and more new jobs are requested to be created by the society. To respond to these trend and request, Incheon International Airport Corporation (IIAC) is about to expand its processing capability with a new terminal; 54 million passengers to 72 million. IIAC is also introducing new strategies such as open innovation (OI) and creating shared value (CSV). With these changes, IIAC faces new challenges of restructuring its business and organization with new ideas. Most organizations including IIAC are becoming more dependent on external resources to keep their competitive advantages under the turbulence of global business environmental changes. Therefore, they focus on the OI paradigm which is reported as a convincing strategy to improve competitiveness in terms of budget and time-to-market. OI is to quickly react to the rapidly changing business environment and is adopted to support startup incubation. In the previous research with IIAC, three major tasks were defined; utilizing the IIAC brand power for external vendors, building a technology road-map, and introducing a collaboration support system. This paper deals with the collaboration system as proposed in the previous research. We focused on the collaboration process for startup incubation. Cases were studied; the K-startup model by the government, a university model to explore youth startups, and a R&D institute model to study professional startups. Based on the case studies, we defined an IIAC model and proposed issues to take care of. The model is distinguished from the other studied models since IIAC is a prospective customer of new technology.

Compared to the early 2000s, the number of tourists in Korea has increased significantly. Approximately 40 million tourists visit Korea each year. In line with this trend, various technologies for developing tourism content have reached a very high level. Therefore, adding experience-oriented AR (Augmented Reality) game based on LBS (Location-Based System) to existing tourist contents will create a new tourism content platform that can give new experience to tourists . This paper proposes a tourism contents platform based on Suwon Hwaseong Fortress which can give such a new experience.

본 연구는 온라인 큐레이션 플랫폼을 이용한 외현적 정보의 제공이 현대미술에 대한 일반대중의 진입장벽을 낮추는 효과를 가지는지 알아보았다. 현대미술은 일반대중이 접근하기 난해한 장르로 여겨져 왔다. 한편, 미술 감상에 대한 연구들은 외현적인 정보의 제공이 미학적 감상 경험에 긍정적인 영향을 줄 수 있음을 밝혔다. 따라서 본 연구에서는 작품에 대한 구체적 정보를 제공하는 것이 현대미술을 감상하는 데에 긍정적인 영향을 미칠 수 있는지 알아보았으며 감상 경험을 심리학적으로 분석한 Leder et al.(2004)의 이론적 모델에 기초하여 외현적 정보가 어떠한 인지적 처리과정을 도와주는지 분석하였다. 더 나아가 최근의 전시 트렌드를 반영하여 온라인 플랫폼 환경을 적용하였다. 실험 1에서는 작품과 설명문이 제공되었고 작품에 대한 이해도, 관람의향을 보고하였다. 실험 결과, 해설이 제시된 조건에서 작품에 대한 이해도가 더 높았다. 또한, 긴 해설이 제시된 조건에서 해설 이해도와 관람의향을 작품이해도가 완전매개하는 매개모형이 나타났다. 실험 2에서는 ARS 설문을 추가하고 온라인 큐레이션의 능동적인 조작 환경을 재현했다. 실험결과, 해설이 제공된 조건들에서 관람의향, 작품이해도가 더 높았다. 예술사적 의미, 자아성찰, 창의성 등 Leder et al.(2004) 모델의 후기 단계에 해당하는 지표들 역시 해설조건에서 더 높은 것으로 나타났다. 종합하면, 온라인 큐레이션 환경에서의 정보전달이 일반대중의 현대미술 작품에 대한 인지적 처리와 판단에 도움을 줄 수 있었다.

This study introduces coding education using functional computer game and board game. We direct compare two different types of the educational game for coding education and their learning achievements. The purpose of this study is mainly to compare the effect of the difference of game type on the learner's attitude, learning immersion, education satisfaction and preference for elementary school and junior high school students. In this study, we considered the effect of game platform type on learning and we suggested that when designing an educational game for coding, the proper combination of these platforms are important.

ABSTRACT The growth of the social platform has led to a rapid increase in sustainable value cocreation activities with customers in order to know what a customer wants. In this study, we have studied about co-creation experience in social platform service. Previous prior studies have systematically classify co-creation between customer and enterprise as co-product and value-in-use, Conceptual factors. The co-creation and value-in-use factors theoretically derived from previous researches attempt to investigate the relationship between value co-creation experience and online service quality. We also tried to investigate the relationship between value co-creation experience and online service quality, customer equity, and online word of mouth. To do this, social platform services are divided into open sns and closed sns. Open SNS was targeted to Facebook, Twitter, and Pintrest users. Closed sns were targeted to Instagram, Cyworld, Band, and KakaoTalk users. In this study, it was confirmed that the relationship between online service quality, customer equity, and online word of mouth is significant through value co - creation experience. In the future, companies that design social platforms will be asked to do what they should do to co-create with customers, and provide suggestions for how to operate the platform in order to continuously activate value co-creative experiences.

The concept of „Sustainability‟ has become as major concern and it used by consumers and corporations to convey the concept of taking care of the environment. Environmental concern has led to sustainable consumption in a variety of product categories, such as electricity, textiles, apparel, food, and grocery products (Chan, 2001; Harrison, Newholm, & Shaw, 2005; Vermeir & Verbeke, 2006a, 2006b). Interest of the negative environmental impacts are rapidly increasing in present fashion business and consumer behavior has become a rising concern of the consumption and fashion supply chain to apply sustainable consumption (Birtwistle & Moore, 2007; Fineman, 2001). The environmental and social concern recognized in fashion industry from 1990‟s. However, the complexity of conceptual definition of sustainability and ecologically responsible consumer generates different and mistaken perception to consumer. In addition, in fashion industry, the terms of „eco-fashion‟, „environmentally friendly fashion‟,„green fashion‟, „ethical fashion‟, and „sustainable fashion‟ are frequently used interchangeably to describe the same concept. These interchangeable terminology is leading to confusion of the readers by the non-unified terminology (Choi et al., 2012). Also, consumers seem to have narrow scope and little understanding of sustainable fashion. In general, consumers focuses on environmental aspect not the wide-range of complexity of environment, social, and economical concern (Cervellon, Hjerth, Ricard, & Carey, 2010). The growing number of fashion brands are leveraging on green branding initiatives. Green marketing is increasing rapidly in corporate aspects and for a consumer perspective, global consumers are recognizing a personal accountability to take responsibility for social and environmental issues. Despite the fact many of individuals‟ willingness to purchase green products has increased in the last few years, however, there is limited studies suggest that purchase of green or sustainable products. Consumer research on sustainable fashion has mainly focused on consumer behaviors towards sustainable fashion products (SFPs); however, relevant studies that examined the whole process of the predicting proenvironmental behavior cross nationally value and the eWOM are still scarce. The purposes of research model are 1) to identify the determinants of eWOM intention on consumers' purchase intentions, 2) to examine the information adoption process as precursors of purchase intention of sustainable fashion, and 3) to testify different message types effects to information adoption process.

This study focuses on the value of fashion social platform and investigates the relationships between knowledge sharing and customer value. The study examines the effects of social network attributes on knowledge sharing in social platforms and the effects of knowledge sharing on customer value in social platforms. In the context of a fashion social platform, this study clarifies the concept of customer value, the role of knowledge sharing, and the relationships between knowledge sharing and customer value. The study builds a theoretical model regarding fashion social platforms and customer value that offers implications for fashion management practitioners.

Current marine navigational practice relies less on long-range visual marine signals such as lighthouses for reference purposes. This is due to the availability of Global Navigation Satellite Systems (GNSS), which are integrated with other navigational aids on ships. Therefore, the objective of this study is to review the function of Pisang Island lighthouse and to propose the most relevant use of Pisang Island for current navigational needs. The function of the lighthouse was reviewed according to the IALA Navigational Guide and the AIS data image. The result showed that the most suitable navigational use of the lighthouse is to act as a reference for Line of Position (LOP). The AIS data image indicated that mariners are not using Pisang Island lighthouse for LOP. The trend in the Straits of Malacca (SoM) was compared with the trend in the Straits of Dover, UK. The selected experts verified that LOP was not practised there. As a specific example, a tanker ship route in the South China Sea was used to further support that LOP was not practised. This evidence supported the view that Pisang Island lighthouse is less relevant for current navigational practice and does not directly support the coastal state VTS operation and the establishment of the marine electronic highway. Furthermore, the existing shore-based VTS radar has limitations on range and the detection of targets near Pisang Island. Therefore, this study proposes the establishment of a new radar station on Pisang Island at the existing site of the lighthouse. The proposed new radar station on Pisang Island will add to the existing coverage of the VTS radar, bridging the coverage gaps to overcome the weakness of the existing shore-based radar and improve the safety and security of marine navigation in the SoM.

With rapid advances in science and technology, there is a greater demand and need to revolutionize higher education with strong emphasis on creative convergence. Lately STEAM (Science, Technology, Engineering, Arts and Mathematics) has been deemed as a possible solution to stimulate creative convergent education. Aligning with STEAM pedagogical philosophy, this paper presents a case of game jam as an alternative educational platform that helps to encourage creativity and collaboration across diverse cultures and disciplinary boundaries at the global level. Game jam is a social event at which programmers, graphic designers, producers, and game mangers work together to make a playable game within given time constraints. Based on empirical experimentation with and observation on global applied game jam over the past few years, this paper suggests that global applied game can serve as a creative convergent educational platform within and beyond traditional classroom settings.

This paper presents a vibration mitigation method for a railroad station using dual layer TMD (Tuned Mass Damper) technology. The proposed technology can be applied to a platform by installing a spring-damper system between the platform and the railroad station structure. Especially, considering passenger comfort, the technology is developed for not just reducing the vibration of the railroad station structure but minimizing the vibration of the platform. The technology consists of two serially connected masses, and the vibration of the top mass is designed to be minimized, while the bottom mass serves as the platform TMD. The design parameters such as the spring constant and damping coefficient of the dual layer TMD system are optimized using the acceleration response of the structure, at which the platform is installed. The feasibility and performance of the vibration mitigation method is verified via a numerical example of a simple beam structure subjected to a moving load, which can be analogized as a railroad station with a moving train on top. The result of the numerical study shows that the proposed method can effectively reduces the vibration of the top mass as well as the beam structure at the design speed of the moving load.

Safety flight training can be done, through the platform proposed in this paper. This paper designed a flight dynamic model and identified essential functions in order to enable pilots to simulate a training environment similar to the actual. It also design activity diagram, concept as well as class diagram. This paper presents the main features and direction of aircraft to be equipped in the future standard platforms. By design main class of flight dynamic and description. it will help developer to setup the standard platform for aircraft simulation.

Ocean economy plays a crucial role in the strengthening maritime safety industry and in the welfare of human beings. Electric Submersible Pumps (ESP) have been widely used in floating platforms on the sea to provide oil for machines. However, the ESP fault may lead to ocean environment pollution, on the other hand, a timely fault diagnosis of ESP can improve the ocean economy. In order to meet the strict regulations of the ocean economy and environmental protection, the fault diagnosis of ESP system has become more and more popular in many countries. The vibration mechanical models of typical faults have been able to successfully diagnose the faults of ESP. And different types of sensors are used to monitor the vibration signal for the signal analysis and fault diagnosis in the ESP system. Meanwhile, physical sensors would increase the fault diagnosis challenge. Nowadays, the method of neural network for the fault diagnosis of ESP has been applied widely, which can diagnose the fault of an electric pump accurately based on the large database. To reduce the number of sensors and to avoid the large database, in this paper, algorithms are designed based on feature extraction to diagnose the fault of the ESP system. Simulation results show that the algorithms can achieve the prospective objectives superbly.

The study presents technology which can decrease the vibration of railroad station via adopting a friction bearing system beneath a platform. The platform itself then constitutes a tuned mass damper system (TMD) as the bearing provides stiffness and damping of the TMD system. To increase the robustness of the TMD system, the bearing system with adjustable stiffness is utilized. The vibration reduction performance of the TMD system is verified via numerical analysis on an elevated railroad station known as having a structural type with highest noise and vibration level. The numerical analysis is performed using a commercial program, ABAQUS. The vibration analysis is performed considering vehicle-track-structure interaction. The result of the numerical analysis shows that the TMD technology can reduce significant amount of vibration of a railroad station.

A very unique case is found in the architectural remains related to the north and south square-platform at the lecture hall site of Hwangnyongsa, that of Anyangsa, and the estimated lecture hall site of Beopsusa as they are not observed in the other lecture halls. The platform has been discovered at only those three Buddhist temples from Goryeo, and its case has not been found in ancient Buddhist temples of China and Japan. This study thus set out to investigate the north and south square-platform at lecture hall sites by examining in details its architectural remains at the three Buddhist temples and reviewing the Buddhist literature about the lecture halls of ancient Buddhist temples. Based on the findings, it was estimated that the architectural remains of north and south square-platform at the lecture hall sites of Buddhist temples were those of platform for Buddhist sermons or those of high chair platform. While the north square-platform involved the remains of north high chair for the Lecturer that gave lectures on the Buddhist scriptures, the south one did those of south high chair for the Reader who recited the Buddhist scriptures.

삼차원(3D) 프린터의 개발과 보급에 따라 현대의 소비자들은 자신이 원하는 제품을 집에서 3D프린팅 할 수 있는 시대에 살고 있다. 3D프린팅을 통해 소비자 맞춤형 제작이 활성화되기 위해서는 3D프린터와 호환되는 디지털 설계도 를 소비자 스스로 제작할 수 있어야 한다. 또한 최적의 프린팅 재질과 온도를 설정할 수 있어야 하고 3D프린터의 유지보수에 비용을 감당해야 한다. 더하여 혹시 있을지 모르는 디자인에 대한 지식재산권 문제에 대응할 수 있어야 한다. 그러나 실상 소비자가 이러한 문제들에 대응하기는 어렵기 때문에 소비자가 원하는 방향으로 3D프린팅을 대행해 줄 수 있는 서비스가 개발될 필요가 있다. 이런 동기에서 출발한 본 연구는 소비자가 원하는 3D프린팅 서비스 방식이 무엇인지에 통찰을 얻기 위해 세계적인 온라인 3D프린팅 제품판매 업체인 Shapeways (www.shapeways.com)에 대한 사용자 경험을 다양한 측면에서 탐색하였다. 그 결과 Shapeways의 사용성, 검색과정, 가격 적절성, 재방문의도, 디자인 의 다양성, 디자인 만족도에 대한 양적 평가에서 전반적으로 낮은 평가를 받았다. 또한 다양한 언어지원, 제품 제작과정 공개 및 온-오프라인 사이트 동시운영, 디자이너 중심의 소비자 맞춤형 제작 서비스, 배송 안전성과 제품 내구성에 대한 확신을 줄 수 있는 서비스, 표면의 마감처리 서비스가 필요하다는 건설적인 의견을 통해 소비자 맞춤형 3D프린팅 서비스에 대한 통찰을 획득하였다. 본 연구가 3D프린팅 서비스 플랫폼 개발뿐 아니라, 관련된 산업 및 연구에도 폭넓은 시사점을 줄 것으로 기대한다.

이 논문은 최근 경영학에서 많이 연구되고 있는 플랫폼전략에 근거하여 전통 문화마을의 생태계를 연구 하고 있다. 전통 문화마을이 국내 관광의 중요한 명소로서 개발되고 있는데, 공진화의 플랫폼전략 관점 에서 분석되고 발전될 필요가 있다. 문화생태계에 대한 플랫폼전략은 관광산업에서 매우 유용할 것이다. 이 논문은 국내 한옥마을 중 전통 문화의 콘텐츠가 잘 활용되고 있는 전주 한옥마을을 중심으로 문화생 태계의 플랫폼을 분석하고 있다. 전주 한옥마을이라는 플랫폼을 중심으로 SPICE 모델과 CPNT 모델을 적용하고 있다. 한국의 전통문화는 한국이라는 브랜드를 세계 속에 알릴 수 있고, 이와 더불어 파생되는 다양한 비즈니스의 효과도 매우 클 것이다. 문화생태계의 이해를 통하여, 관광산업의 발전에 도움을 줄 수 있다. 이 논문에서 거시적 관점으로 적용되고 있는 플랫폼 개념이 문화생태계에서도 활용된다면 큰 의미가 있을 것이다.