This study was operated to establish induction using ultrasonography by estimating the relation of follicle size and estrus manifestation. Clinical estrus symptoms were observed 97.4% in cows and 87.5% in heifers when overall 55 cows were induced to estrus in a single dose of after verifying CL through ultrasonography, which means estrus hours among those 52 cows showing the clinical estrus symptoms were estimated 2.39 days on cows and for 2.37 days on heifers which showed no differences (p>0.05). The estrus manifestation hours according to the follicle size in cows didn't have any significance each other (p>0.05), though estrus hours was 54 hours (the shortest) with follicle size bigger than 10 mm and were made up within 69 hours. The estrus manifestation hours according to the follicle size in heifers didn't have any significance each other (p>0.05) and took around 42 hours (the shortest) with follicle size of 5mm (the smallest) and were made up within 66 hours. Follicles after injection were ovulated and assigned to many phases as follows; Group 1 (growing phase) - continuously growing into ovulation, Group 2 (growing and static phase) - delaying in growth after the growth of follicles, Group 3 (static and growing phase) - growing after growth delay, Group 4 (regressing and new growing phase) - the follicle is closed and a new follicle grows. In addition, the process of follicle development and estrus hours had no significance each other (p>0.05), though estrus manifestation hours in Group 1 and 2 was relatively short, and in Group 3 and 4 for a relatively long time. In the result of all above, the estrus manifestation hours after injection has no differences accoring to the follicle size in cows and heifers. Therefore, High pregnancy rate is obtained when practicing artificial insemination within 3 days in estrus or TAI in 72 to 80 hours after adminitrating .

국외에서 승용말 번식에서 인공수정 및 수정란이식의 활용도가 높지만, 국내에서 사육 되는 말은 대부분이 경주마로 자연종부로만 번식을 하고 있다. 인공수정 등 번식기술의 국산화를 위해서는 발정주기의 난포 변화와 난포 변화에 따른 호르몬 변화 등의 연구가 필요하다. 본 연구에서는 말 발정기의 난포크기에 따른 프로제스테론과 에스트로젠의 변 화 및 난포 크기에 따른 배란유도와 인공수정 결과를 조사하였다. 말의 난포 크기가 발정증상 개시부터 2 cm, 3 cm 및 4 cm 이상에서 혈액내 프로제스 테론과 에스트로젠을 분석하였다. 발정개시 후 난포의 크기에 따라 hCG를 이용 배란을 유도하였고, 배란 유도 후 24 및 40시간에 동결정액으로 2회 인공수정 하였다. 발정기의 말에서 혈액내 프로제스테론 농도는 난포의 크기가 각각 2 cm, 3 cm 및 4 cm에서 평균 9.09 ng/ml, 13.04 ng.ml 및 1.40 ng/ml로서 4 cm에서 급격하게 감소하였 다. 한편 에스트로젠농도는 각각 평균 59.20 pg/ml, 48.5 pg/ml, 및 41.07 pg/ml로서 차이 가 없었다. 배란유도 시점의 난포 크기가 임신율에 미치는 영향을 조사한 결과 난포 크 기가 4 cm 미만에서는 임신율이 0%였으나, 4 cm 이상에서는 60%였다. 배란유도 후 인 공수정 시점의 난포 크기가 임신율에 미치는 효과를 조사한 결과 난포 크기가 5 cm 미 만은 임신에 실패하였으나, 5 cm 이상에서 60%의 임신율을 나타냈다. 본 연구를 통하여 발정기 말의 배란유도 및 인공수정 시점에 주기적인 초음파 검사를 통한 난포 크기의 확인이 인공수정의 임신율에 영향을 미치는 것으로 판단된다.

본 연구는 도축된 제주마의 난소를 이용하여 초음파 유도난포란 채취 기술을 확립하고자 난소, 난포 및 황체의 크기를 초음파상과 육안적 측정치를 비교하고자 하였다. 초음파상 측정치와 caliper를 이용한 실제 크기의 측정치 비교에서는 통계학적 차이는 보이지 않았다. 배란 직전의 난포를 조사한 결과, 초음파상에서 관찰한 것은 난소 한 개 당 평균 0.83개와 평균 크기는 2.86 m였으며, 육안으로 관찰된 것은 난소 한 개 당 평균 0.75개와 평균 크기는

This study was conducted to find out the effect of follicle size and oocyte type on in vitro maturation of poricine follicular oocytes. TCM-HEPEAS medium was used to basic medium, and the oocyte matured in vitro was stained with the Rapid staining method. The results obtained were summarized as follows; 1. The number of follicles an ovary was 20.5. The number of A-and B-typed oocytes an ovary was 2.34. The proportion of A-and b-types oocytes was 40% of the recovery oocytes. 2. Cumulus expanison indexes(CEI) by the follicle size were 1.62∼2.34(<2mm), 1.27∼2.28(2∼5mm) and 1.46∼2.75(>5mm). It was no differ to maturation rate by the follicle size. 3. The degree of oocyte maturation based on oocyte type did not differ for B-and C-typed oocyted but the index of oocyte type A was higher than that of b-and C-typed oocytes. 4. When follicluar oocytes were cultured for 42 hours, the proportion of the Met-II(second metaphase) stage were 22.5% (degree 1), 35.4%(degree 2) and 65.5% (degree 3).

This study was carried out to compare the actual size(length and height) of ovaries, follicles and corpora lutea of Korean native cow with those on sonograms. We used 3 different probes(3.5 MHz abdominal probe, 6.5 MHz transvaginal probe and 5.0 MHz transrectal probe) and a calipher for measurements of ovaries, follicles and corpora lutea on sonograms and actual size. Under water immersion, 157 ovaries were scanned with 3 probes and measured in actual size and compared each other. The average height and width of ovaries of Korean native cows were 17.403.99 and 34.236.02mm, respectively. In comparison of height, length of ovaries and preovulation follicles, we found that image with a transvaginal probe was nearly the same as the actual size(p<0.01), but with an abdominal probe the image was appeared larger than the actual size. In measurement(diameter) of preovulation follicles the transvaginal probe was proven to be more accurate to the actual size than other probes and in corpus luteum measurement all probes were accurate. In the comparison of number of follicles by different size ranges, there was no statistical difference in the count of follicles over 10 mm in diameter between the transvaginal probe and naked eyes.