Background: In healthy dentin conditions, odontoblasts have an important role such as protection from invasion of pathogens. In mammalian teeth, progenitors such as mesenchymal stem cells (MSCs) can migrate and differentiate into odontoblast-like cells, leading to the formation of reparative dentin. For differentiation using stem cells, it is crucial to provide conditions similar to the complex and intricate in vivo environment. The purpose of this study was to evaluate the potential of differentiation into odonto/ osteoblasts, and compare co-culture with/without epithelial cells. Methods: MSCs and epithelial cells were successfully isolated from dental tissues. We investigated the influences of epithelial cells on the differentiation process of dental pulp stem cells into odonto/osteoblasts using co-culture systems. The differentiation potential with/without epithelial cells was analyzed for the expression of specific markers and calcium contents. Results: Differentiated odonto/osteoblast derived from dental pulp tissue-derived mesenchymal stem cells with/without epithelial cells were evaluated by qRT-PCR, immunostaining, calcium content, and ALP staining. The expression of odonto/ osteoblast-specific markers, calcium content, and ALP staining intensity were significantly increased in differentiated cells. Moreover, the odonto/osteogenic differentiation capacity with epithelial cells co-culture was significantly higher than without epithelial cells co-culture. Conclusions: These results suggest that odonto/osteogenic differentiation co-cultured with epithelial cells has a more efficient application.

The paper presents the experimental investigation of RC beams retrofitted with Textile Reinforced Mortar (TRM), featuring enhanced bond capacity. Anchoring systems, including an extension of retrofitting length and the use of chemical anchors, are newly employed to improve the structural performance of the RC beam retrofitted with TRM. For the experimental investigation, a total of seven shear-critical RC beams, with and without stirrups, were designed and constructed. The structural behaviors of specimens retrofitted with the proposed TRM methods were compared to those of non-retrofitted specimens or specimens strengthened with conventional TRM methods. Crack pattern, force-displacement relationship, and absorbed energy were evaluated for each specimen. The experimental results indicate a significant improvement in the shear capacity of the RC beam with the proposed retrofitting method. Therefore, it is concluded that the application of an extended retrofitting length and chemical anchors to the TRM retrofitting method can effectively enhance the bond capacity of TRM, thereby improving the shear performance of RC beams.

Economical radioactive soil treatment technology is essential to safely and efficiently treat of high-concentration radioactive areas and contaminated sites during operation of nuclear power plants at home and abroad. This study is to determine the performance of BERAD (Beautiful Environmental construction’s RAdioactive soil Decontamination system) before applying magnetic nanoparticles and adsorbents developed by the KAERI (Korea Atomic Energy Research Institute) which will be used in the national funded project to a large-capacity radioactive soil decontamination system. BERAD uses Soil Washing Process by US EPA (402-R-007-004 (2007)) and can decontaminate 0.5 tons of radioactive soil per hour through water washing and/or chemical washing with particle size separation. When contaminated soil is input to BERAD, the soil is selected and washed, and after going through a rinse stage and particle size separation stage, it discharges decontaminated soil separated by sludge of less than 0.075 mm. In this experiment, the concentrations of four general isotopes (A, B, C, and D which are important radioisotopes when soil is contaminated by them.) were analyzed by using ICP-MS to compare before and after decontamination by BERAD. Since BERAD is the commercial-scale pilot system that decontaminates relatively large amount of soil, so it is difficult to test using radioactive isotopes. So important general elements such as A, B, C, and D in soil were analyzed. In the study, BERAD decontaminated soil by using water washing. And the particle size of soil was divided into a total of six particle size sections with five sieves: 4 mm, 2 mm, 0.850 mm, 0.212 mm, and 0.075 mm. Concentrations of A, B, C, and D in the soil particles larger than 4 mm are almost the lowest regardless of before and after decontamination by BERAD. For soil particles less than 4 mm, the concentrations of C and D decreased constantly after BERAD decontamination. On the other hand, the decontamination efficiency of A and B decreased as the soil particle became smaller, but the concentrations of A and B increased for the soil particle below 0.075 mm. As a result, decontamination efficiency of one cycle using BERAD for all nuclides in soil particles between 4 mm and 0.075 mm is about 45% to 65 %.

최근 국내에서 연간 지진 발생 횟수가 꾸준히 증가함에 따라 공공시설물에 대한 내진 보강의 필요성이 더욱 대두되고 있다. 이 연구 에서는 사각 단면을 가진 철근콘크리트 기둥에서 강봉보강의 유무에 따른 내진 성능 개선 효과를 분석하기 위해 비선형 유한요소해 석을 수행하였으며, 검증을 위해 구조실험결과와 비교하였다. 분석 결과, 이 연구에서 수행한 유한요소해석이 실제 강봉보강공법을 적용한 철근콘크리트 기둥의 구조 거동을 합리적으로 잘 묘사하는 것으로 나타났다. 또한, 해석 및 실험 모두 강봉보강공법 적용으로 인해 파괴모드가 취성파괴에서 연성파괴로 전환되었으며, 강도와 연성도 모두 증가하는 것으로 나타났다. 따라서, 강봉보강공법 적 용을 통해 기존 철근콘크리트 기둥의 내진 성능을 효과적으로 증진시킬 수 있는 것으로 판단된다. 이 연구의 주요 결과는 향후 설계 방 안 마련 등 관련 연구에 유용할 것으로 기대된다.

Various radionuclides are released and contaminate soils by the nuclear accidents, nuclear tests and disposal of radioactive waste. Among radionuclides, 137Cs is a harmful radioactive element that emits high-energy β particles and γ rays with a half-life of 30.2 years. 137Cs is difficult to extract because it is fixed to soil particles. For the volume reduction technology development of contaminated soil, this study tried to evaluate the irreversible Cs adsorption capacity of granite-originated soil. The soil sample used in the study was collected from C horizon of the soil developed in Mesozoic mica granite. The soil texture, mineralogy, organic content, pH, EC, cation exchange capacity (CEC), water-soluble cation and anion content of the soil samples were determined. A kinetic adsorption experiment and an isotherm adsorption experiment were performed to understand the overall Cs adsorption characteristics using 133Cs. The desorption of Cs by 0.1 mM KCl was also tested for the sample spiked with 133Cs and 137Cs. The soil sample showed a pH of 6.73, EC of 24.50 μS cm-1, and CEC of 1.34 cmolc kg-1, organic matter of 0.53% and sandy loam in texture. Quartz, feldspar and mica were identified as the major mineral components of bulk sample. The clay fraction consists of mica, hydroxyl-interlayer vermiculite (HIV), vermiculite and kaolinite. In the kinetic adsorption experiment, the Cs adsorption showed fast adsorption rates at the initial stage (6 hours) regardless of the 133Cs concentration, and the adsorption equilibrium state was reached after 48 hours. It was the most suitable for the pseudo second-order model. The 133Cs adsorption increased nonlinearly from low to high concentration, which was well match with the dual site Langmuir model. As a result of the desorption experiment, desorption was not performed up to 1.1 mg kg-1 in the presence of competitive ions K+, which is about 0.035% of CEC calculated by the isotherm model. The adsorption of Cs was controlled by frayed edge sites (FES) at a low concentrations and by basal sites or interlayer sites at a high concentration. Irreversible Cs fixation of by FES may be contributed by mainly weathered mica, and when these minerals are separated from the granite origin soil, the possibility of reducing the contamination concentration and volume of radioactive soil waste can be expected.

The safety of a KTC-360 transport cask, a large-capacity pressurized heavy-water reactor transport cask that transports CANDU spent nuclear fuel discharged from the reactor after burning in a pressurized heavy-water reactor, must be demonstrated under the normal transport and accident conditions specified under transport cask regulations. To confirm the thermal integrity of this cask under normal transport and accident conditions, high-temperature and fire tests were performed using a one-third slice model of an actual KTC-360 cask. The results revealed that the surface temperature of the cask was 62°C, indicating that such casks must be transported separately. The highest temperature of the CANDU spent nuclear fuel was predicted to be lower than the melting temperature of Zircaloy-4, which was the sheath material used. Therefore, if normal operating conditions are applied, the thermal integrity of a KTC-360 cask can be maintained under normal transport conditions. The fire test revealed that the maximum temperatures of the structural materials, stainless steel, and carbon steel were 446°C lower than the permitted maximum temperatures, proving the thermal integrity of the cask under fire accident conditions.

In this paper, the flexural capacity equation of FRP-bar reinforced concrete beams was verified by comparing the experimental results and flexural capacity obtained according to the ACI procedure. And, also the economic feasibility of FRP-bar reinforced concrete beams was analyzed by comparing nominal moment capacity of beams. The results of analysis were as follows, 1) GFRP concrete beams have lower flexural performance than reinforced concrete beams, whereas CFRP concrete beams have similar flexural performance to reinforced concrete beams under the same reinforcement ratio 2) Although the design moment increased as the compressive strength of concrete increased, the flexural performance of GFRP reinforced concrete beams was found to be lower than the reinforced concrete beams for all reinforcement ratios.

목적 : 본 연구는 한국표준산업분류에 따라 산재환자의 작업능력평가 세부항목 실시 데이터를 분석하여 업종별 직장복귀프로그램의 기초자료를 제시하는데 있다. 연구방법 : 후향적 코호트 조사 연구로 근로복지공단 소속병원의 전자의무기록을 활용하여 2018년 1월부터 2020년 12월까지 작업능력평가를 실시한 2,145명의 산재환자의 기초분석과 한국표준산업분류에 따라 건설업, 제조업, 전문ㆍ기술업, 시설 관리 및 서비스업, 운송업, 보건업 및 사회복지 서비스업으로 분류한 후 대상자들에게 시행된 작업능력평가 세부항목의 수행 빈도 차이를 분석하였다. 결과 : 작업능력평가에 참여한 대상자는 남성 1,798명(83.8%), 여성 347명(16.2%)이었으며, 연령대는 50-59세가 790명(36.8%)으로 가장 많았다. 산재 요양시작 이후 평균8.83 ± 5.32개월 시점에 평가가 이루어졌다. 제조업 692명(32.3%), 건설업 642명(29.9%), 전문ㆍ기술업 308명(14.4%)이 대부분이었고 손상 부위는 하지 손상이 736명(34.3%)으로 가장 많았다. 작업능력평가 시 포함된 세부 평가항목 중 어깨높이에서 밀기ㆍ당기기, 작업장 이동에서 걷기, 기어가기, 자세유지평가에서 무릎 꿇기 작업을 제외한 모든 항목에서 직업에 따라 유의한 차이가 있었다. 특히, 건설업에서 들기와 옮기기의 수행 빈도가 높았고, 가장 무거운 무게까지 평가를 했으며, 제조업에서는 자세유지평가 빈도가 높았다. 결론 : 본 연구에서 작업능력평가에 참여한 산재환자의 동향과 한국표준산업분류에 따라 실시되는 작업능력평가 세부항목 차이를 확인하여 임상에서 참고할 수 있는 기초자료를 제시하였다.

In 2016, an earthquake occurred at Gyeongju, Korea. At the Wolsong site, the observed peak ground acceleration was lower than the operating basis earthquake (OBE) level of Wolsong nuclear power plant. However, the measured spectral acceleration value exceeded the spectral acceleration of the operating-basis earthquake (OBE) level in some sections of the response spectrum, resulting in a manual shutdown of the nuclear power plant. Analysis of the response spectra shape of the Gyeongju earthquake motion showed that the high-frequency components are stronger than the response spectra shape used in nuclear power plant design. Therefore, the seismic performance evaluation of structures and equipment of nuclear power plants should be made to reflect the characteristics of site-specific earthquakes. In general, the floor response spectrum shape at the installation site or the generalized response spectrum shape is used for the seismic performance evaluation of structures and equipment. In this study, a generalized response spectrum shape is proposed for seismic performance evaluation of structures and equipment for nuclear power plants. The proposed response spectrum shape reflects the characteristics of earthquake motion in Korea through earthquake hazard analysis, and it can be applied to structures and equipment at various locations.

PURPOSES: The objective of this study is to evaluate the structural capacity of asphalt pavement in subsurface cavity sections using falling weight deflectometer (FWD) backcalculation method. METHODS: It is necessary to analyze the reduction of structural capacity in asphalt pavements due to the occurrence of subsurface cavities. The FWD testing was conducted on the cavity and intact asphalt pavement in the city of Seoul. The GAPAVE, backcalculation program for FWD deflections, was utilized to determine the layer moduli in asphalt pavements. The remaining life of asphalt pavements in cavity sections were predicted using the pavement performance model for fatigue cracking. The backcalculated layer moduli between cavity and intact sections were compared to determine the reduction of structural capacity due to subsurface cavity. The relationship between the reduction of layer modulus and cavity depth/length was analyzed to estimate the effect of cavity characteristics on the structural capacity degradation. RESULTS: According to the FWD backcalculation results, the modulus of asphalt layer, subbase, and subgrade in cavity sections are generally lower than those in intact sections. In the case of asphalt layers, the backcalculated modulus in cavity section was reduced by 50% compared to intact section. A study for the prediction of remaining life of cavity section shows that the occurrence of subsurface cavity induces the decrease of the pavement life significantly. It is found that there is no close relationship between the backcalculated modulus and cavity length. However, the reduction of asphalt layer modulus is highly correlated with the cavity depth and was found to increase with the decrease of cavity depth. CONCLUSIONS : This reduction of structural capacity due to the occurrence of cavities underneath asphalt pavements was determined using FWD backcalculation analysis. In the future, this approach will be utilized to establish the criteria of road collapse risk and predict the remaining life of cavity sections under numerous varied conditions.

Orius minutus (L.) (Hemiptera: Anthocotidae) is a native predator of mites, thrips, and aphids whereas Orius laevigatus (Fieber) is a commercialized predator were assessed against O. metasequiae in laboratory and field. Adult females of both predator predated equally O. metasequiae adult after 3 hours of exposure in the laboratory on no choice assay. Orius minutus and O. laevigatus released with Portulaca oleracea plant in Metasequouia glyptostroboides tree reduced O. metasequiae population equally by 48.3% and 42.7% on first week after exposure. But O. laevigatus released without P. oleracea plant reduced 52.4% on the first week whereas O. minutus reduced only 6.2% of O. metasequiae population. We found only 3 O. minutus eggs on M. glyptostroboides leaves where 22 O. laevigatus eggs were found when treated without P. oleracea plant. Orius laevigatus laid 160.0 eggs on P. oleracea plant where as O. minutus laid only 25.7 eggs per plant. These result demonstrate that O. laevigatus can be applied for conservation biological control to suppress O. metasequiae population.

This paper is to investigate the micro-behavior of the double-span beams with WUF-W seismic connection under combined axial tension and moment and to propose the rational rotational capacity of it for progressive collapse-resistant analysis and design addressing the stress and strain transfer mechanism. To this end, the behavior of the double-span beams under the column missing event is first investigated using the advanced nonlinear finite element analysis. The characteristics of fracture indices of double-span beams with WUF-W connection under combined axial tension and flexural moment are addressed and then proposed the rational rotational capacity as the basic datum for the progressive collapse-resistant design and analysis. The distribution of fracture indices related to stress and strain for the double-span beams is investigated based on a material and geometric nonlinear finite element analysis. Furthermore, the micro-behavior for earthquake and progressive collapse is explicitly different.

단면증대공법은 경제성과 시공상 보편적으로 사용되는 공법으로 우리나라의 철근콘크리트 기둥을 보강하기 위한 공법으로 널리 사용되어 왔다. 이와 관련하여 단면증대된 부재의 성능에 관한 실험적 또는 해석적 연구는 수행된 사례가 있으나, 휨성능과 단면증대 부분의 표면거칠기 범위의 상관관계에 대한 연구는 수행되지 않았다. 따라서, 본 연구에서는 단면증대된 보의 휨성능을 평가하기 위해 표면거칠기의 범위를 변수로 한 단면증대보의 휨실험을 수행하였다. 그 결과는 다음과 같다. (1) 시공에서 사용되는 방법에 따라 거칠기 처리가 된 표면의 거칠기 이력을 분석한 결과 인접 산과 골의 차이는 6mm를 초과하였다. (2) 거칠기 이력이 측정된 거리가 600mm를 초과하연, 거칠기 지표는 일관된 결과를 나타내었다. (3) 표면거칠기처리를 하지 않은 보의 휨성능과 비교할 때, 표면거칠기가 된 보의 휨강성은 25%증가하고, 항복강도는 9%, 최대강도는 6%증가하였다. (4) 모든 시험편은 표면거칠기 처리의 유무에 상관없이 단면해석결과 나타난 항복강도보다 높은 강도를 나타내었다.