The large copper butterfly Lycaena dispar (Haworth, 1803; Lepidoptera: Lycaenidae) has been categorized as a near-threatened species (NT) in South Korea from 2012 mainly due to limited distribution. In this study, we visited 36 sites spread across all South Korean provinces to verify the distributional range of the species and sequenced mitochondrial COI for 53 individuals from nine sites. We observed L. dispar at 15 sites in six provinces, including the two previously known provinces, indicating a southward range expansion. The in-field monitoring and genetic data collectively suggested that L. dispar does not have a limited distribution nor is it isolated, indicating that it should be reclassified as less vulnerable. Our study demonstrates that the combination of field and genetic data can provide a more reliable assessment of the stability of a species.

복숭아혹진딧물(Myzus persicae)은 다식성으로 담배, 감자, 고추, 배추, 복숭아 등에 심각한 피해를 입히는 대표적인 농업해충이다. 본 연구에 서는 국내 복숭아혹진딧물 야외개체군의 λ-cyhalothrin, imidacloprid 및 flupyradifurone에 대한 약제 저항성 발달 수준과 점 돌연변이(R81T, L1014F, M918L)의 발생 여부를 확인하였다. 또한, qRT-PCR을 통해 사이토크롬 P450 유전자인 CYP6CY3 발현량을 확인하였다. 그 결과, λ -cyhalothrin은 저항성비(Resistance Ratio, RR)가 12개 모든 지역이 > 200으로 높은 저항성을 보였다. Imidacloprid와 flupyradifurone은 YS, UR, HY, 그리고 WJ 개체군에서 > 200의 저항성비로 높은 저항성을 나타냈다. R81T는 12개 집단 중 약 50%, L1014F는 약 33.3%, M918L은 100%에서 발현하였다. 또한 qRT-PCR을 통해 imidacloprid 저항성 개체에서 subunit CYP6CY3의 발현량이 높게 나타난 것을 확인하였다. 이러 한 결과는 M918L 점 돌연변이는 λ-cyhalothrin 저항성 진단마커로, R81T와 CYP6CY3의 높은 발현은 imidacloprid 저항성 진단마커로 활용이 가능하다는 것을 시사한다.

식량 작물의 확보 및 생산량 예측은 국가 발전에 있어 필수적이며, 국가 경제뿐만 아니라 전 세계 식량 안보에 기여 한다. 최근 환경오염으로 인한 이상기후는 식량 작물 생산량에 직ㆍ간접적으로 부정적 영향을 끼치고 있어, 작물 수확량 예측 불확실성이 높아지고 있다. 특히, 노지 작물의 경우 생산량 감소와 품질 저하 문제가 화두 되고 있다. 이러한 문제는 농가들뿐만 아니라 소비자들에게도 큰 피해를 안겨주고 있다. 이러한 생산량 예측 이슈를 해결하기 위해 최근에는 인공지능 기술이 농업 분야에도 활발히 적용되고 있다. 작물 수확량의 정확한 예측을 위한 머신러닝 기반 연구가 집중적으로 수행되고 있다. 따라서, 본 연구에서는 이와 같은 인공지능 기반의 노지 작물 수확량 예측 기술(머신러닝, 딥러닝, 하이브리드 모델 등) 현황 및 작물 수확량에 가장 영향을 많이 끼치는 모델 파라미터 등을 조사하였다.

최근 기후변화로 인해 외래 무척추동물(곤충)의 돌발발생 가능성이 높아지고 있는 추세를 감안할 때 이들에 대한 확산 및 변화예측 진단을 통한 조기방제의 중요성이 대두되고 있다. 그러나, 외래곤충의 종 정보 및 정확한 침입경로가 충분치 않아 문제해충이 될 가능성이 매우 높은 것이 사실이다. 또한, 신속정확한 정책결정을 위해서 는 현장조사자료가 체계적으로 수집되어 종합되어야만 정확한 진단 및 방제에 활용될 것이므로 이에 대한 다양 한 장치가 필요하다. 현재 국내 서식 중인 주요 산림해충 일부를 제외한 종들은 대부분 외래곤충으로 돌발적으로 대발생한 해충이 많이 포함되어 있어 국내에 유입된 외래 곤충에 대한 체계적인 데이터 확보를 통해 정착, 확산, 생태계 영향 예측모델 개발 및 활용시스템의 구축이 요구되는 실정이다. 따라서, 본 연구는 외래 곤충의 전국적인 모니터링을 통해 분포정보 및 생태정보 등의 중요 데이터 수집과 최신 정보를 활용하여 국내 유입된 외래 곤충의 정착, 확산, 생태계 영향 및 변화예측을 위한 전략수립과 모바일 등을 이용한 전자야장의 사용에 대해 논하고자 한다.

Republic of Korea (ROK) is operating the Integrated Environmental Radiation Monitoring Network (IERNet) in preparation for a radioactive emergency based on Article 105 of the Nuclear Safety Act (Monitoring of Nationwide Radioactive Environment). 215 radiation monitoring posts are monitoring a wide area, but their location is fixed, so they can’t cover areas where the post is not equipped around the Nuclear Power Plants (NPPs). For this, a mobile radiation monitoring system was developed using a drone or vehicle. However, there are disadvantages: it is performed only at a specific cycle, and an additional workforce is required. In this study, a radiation monitoring system using public transportation was developed to solve the above problems. Considering the range of dose rates from environmental radiation to high radiation doses in accidents, the detector was designed by combining NaI (TI) (in the low-dose area) and GM detector (in the high-dose area). Field test was conducted by installed on a city bus operated by Yeonggwang-gun to confirm the performance of the radiation monitoring system. As a result of the field test, it was confirmed that data is transmitted from the module to the server program in both directions. Based on this study, it will be possible to improve the radiation monitoring capability near nuclear facilities.

본 연구에서는 2021년 전남, 전북을 대상으로 23개소에서 어류 4종(향어, 비단잉어, 참붕어, 금붕어) 총 1,115마리에 대하여 병원체 감염 여부를 조사하였다. 법정전염병 3종(SVC, Spring viraemia of carp, EUS, Epizootic ulcerative syndrome, and KHVD, Koi herpesvirus disease)을 분 석한 결과, 조사한 모든 시료에서 법정전염병이 검출되지 않아 전라권에서 조사한 잉어과어 류는 청정국 또는 청정지역 선언에 중요한 정보를 제공할 것이다.

2017년과 2018년 노지고추 재배기간인 6~8월 간 전북 고창, 충남 청양, 충북 괴산지역 노지 고추 포장에서 총채벌레의 종류와 발생현황을 조사하였다. 시기별로 총채벌레를 채집하여 동정한 결과 꽃노랑총채벌레 (Frankliniella occidentalis), 대만총채벌레 (F. intonsa), 파총채벌레 (Thrips tabaci)의 발생이 확인되었다. 이들 3종 중 대만총채벌레가 시기별로 가장 발생 밀도가 높은 것으로 보아 우점종으로 보여진다. 총채벌레의 발생은 6월 하순부터 증가하기 시작하여 7월 중순에 밀도가 가장 높았다. 따라서, 노지고추에서 총채벌레 방제전략은 6월 상순부터 모니터링하여 밀도증가 이전에 적절한 방제방법을 동원하여야 할 것이다.

In this study, the odor generated in a livestock farm with 500 heads of finisher breed in 661 m² was monitored during 6 months using a gas sensor, a wired / wireless communication system and database server. Odor unit, ammonia, hydrogen sulfide, and total volatile organic compounds (TVOC) were monitored using the gas sensor. To show the tendency of odorous substances generation, the odor concentration was shown in the graph on a monthly and daily basis. Among the analysis items, the maximum generation of odor was found to be closely related to the generation of hydrogen sulfide. Through observing the daily and monthly trends of odor substances, it was found that each substance was a useful indicator for monitoring odor, because ammonia, hydrogen sulfide, odor and TVOC were increased and decreased in a similar pattern. The odors were highest in the hours of the early morning (00:00-05:00), the evening (18:00-23:00), and the morning (06:00-11:00) in a day. After the use of the microbial agent was discontinued in autumn (October), anaerobic digestion of the manure in a pit proceeded and the amount of hydrogen sulfide increased. Therefore, despite a slight decrease in ammonia production, the odor unit level did not decrease after October but rather was somewhat increased. In the future, the use of the odor monitoring system is expected to improve the efficiency of odor sources management.

Increasing Lepidopteran pests such as Helicoverpa armigera are emerging pests of corn in Korea. They cause huge yield losses and deteriorate the quality of corn crop. We monitored the four major lepidopteran pests in major highland corn growing areas of Korea by employing sex pheromone trap during 2012 to 2015 for understanding those population dynamics. Among these species, H. armigera was found to be the main pest based on the damage intensity (80 ~ 90% of total damage). Some effective insecticides were selected such as Indoxacarb etc. Optimal timing of spraying was estimated at around 70 days after planting (earing season) to control H. armigera. Additional, two species of parasitoids (Therion circumflexum and Ophioninae sp.) was identified from H. armigera. However, more extensive survey for natural enemies is needed to set the natural enemy based control program.

PURPOSES: Performance evaluation of four types of asphalt concrete overlays for deteriorated national highways. METHODS : Pavement distress surveys for crack rate and rut depth have been conducted annually using an automated pavement survey vehicle since 2007. Linear and non-linear performance prediction models of the asphalt concrete overlays were developed for 43 sections. The service life of the asphalt overlays was defined as the number of years after which a crack rate of 30% or rut depth of 15mm is observed. RESULTS: The service life of the asphalt overlays was estimated as 17.4 years on an average. In 90.7% of the sections, the service life of the overlays was 15 years or more which is 1.5 times the life of conventional asphalt concrete overlays used in national highways. The performance of the overlays was dependent on the type of asphalt mixture, traffic volume levels, and environmental conditions. CONCLUSIONS : The usage of stone mastic asphalt (SMA) and polymer-modified asphalt (PMA) for the overlays provided good resistance to cracking and rutting development. It is recommended that appropriate asphalt concrete overlays must be applied depending on the type of existing pavement distress.

The cotton aphid, Aphis gossypii (Glover) (Hemiptera:Aphididae), is a highly polyphagous pest that directly or indirectly damages cultivated plants. Six field-collected populations of cotton aphid, A. gossypii (BY-A, BY-B, YJ-A, YJ-B, CJ-A, and CJ-B) were tested for susceptibility to 14 different insecticides. Most population exhibited high to very high levels of resistance to neonicotinoid. Among them, a strain showing resistance to imidacloprid were selected and showed 1,543-fold in resistance as compared with susceptible strain. Piperonyl butoxide (PBO), diethyl maleate (DEM), and S,S,S tributyl-phosphorothiolate (DEF) failed to synergize imidacloprid in this resistant population. In addition, the activity of detoxification enzymes (P450, EST, GST) were no differences between susceptibility and imidalcoprid resistance strain. However, by analyzing the nicotinic acetylcholine receptor β1 subunit loop D, R81T point mutation was detected in BY-A, BY-B, YJ-A, and YJ-B strain.

1. 본 연구에서는 GM 유채(Brassica napus)의 환경방출실험시의 안전관리를 위한 모니터링을 수행하였다. 2. 문헌조사를 통해 유채의 품종 순도 유지를 위한 격리거리와 교잡가능한 종을 확인함으로써 모니터링의 범위와 모니터링 대상 식물종을 결정하였다. 3. GM 유채의 격리포장으로부터 1.3 km 거리 범위 내에서 발견된 B. napus, B. juncea 및 B. oleracea를 대상으로 모니터링을 실시한 결과, 교잡을 통한 유전자의 유출은 발견되지 않았다.

The green peach aphid (Myzus persicae) is a serious pest of agricultural and horticultural crops all over the world. M. persica has rapidly developed resistance to a wide variety of insecticides, including carbamates. The E4/FE4 carboxylesterase is known to be involved in carbamate resistance. To compare the E4/FE4 carboxylesterase gene copy number, as a genetic resistance marker, between seven field strains, quantitative real-time PCR (qPCR) was performed. In addition, quantitative sequencing (QS) was employed to predict the frequencies of acetylcholinesterase (AChE) mutations (A301S and S431F) that are associated with target site insensitivity. All M. persica strains examined possessed the S431F mutation in the heterozygous state except for a susceptible strain, implying the possibility of AChE duplication. In contrast, no A301S mutation was found. Frequency prediction equation was generated from the plots of signal ratios and amplification critical time, which showed a high correlation (r2>0.996). QS analysis of M. persicae populations revealed that the allele frequency of S431F ranged 4% to 63%. Taken together, the AChE resistance allele frequencies determined by QS and the E4/FE4 gene copy number by qPCR should facilitate the detection and monitoring of carbamate resistance in M. persicae in the field.