Sea-buckthorn fruits (Hippophae rhamnoides L.) are used in Mongolia as traditional medicine due to the health-benefits associated with its bioactive components. The purpose of this study was to investigate the chemical composition of Mongolian sea-buckthorn fruits. In terms of proximate composition, crude fat content was the highest, whereas its crude ash content was the lowest. In organic acid contents, malic acid content ( mg/g) was the highest. Free sugars were composed of sucrose, xylose, glucose and mannitol. Vitamin C and carotenoid content were 320 mg/100 g and 715.25 mg/100 g, respectively. The major fatty acids were palmitic (C16:0), palmitoleic (C16:1), caprylic (C8:0) and linoleic (C18:2) acid.

Background : Hippophae rhamnoides L. are known for antioxidant, immunodeficiency, skin protection, influenza infection and prevention of heart disease. This study was carried out to confirm the possibility of functional food by changing the antioxidant effect using H. rhamnoides L. leaf extracts to the Gamju (sweet rice drink). Methods and Results : A total of 12 samples were made of different processes. Briefly, the H. rhamnoides L. leaf were extracted at 60℃ in two different conditions (EtOH 100%, water 100%). Gamju was fermented into three different koji (Aspergillus oryzae – red, yellow, black). In addition, The addition of H. rhamnoides L. leaf extracts were mixed in two ways (simultaneous saccharification, mixed after saccharification). Antioxidant activities were estimated by 2,2-diphenyl-1-picryl-hydrazil (DPPH) and reducing power assay. Total phenolic content (TPC) was determined by Folin-Ciocalteu method. In this study, we found that Gamju mixed with H. rhamnoides L. leaf increased antioxidant effects and TPC than the control (original Gamju). Moreover, the anti-oxidant effects of the mixed H. rhamnoides L. leaf with Gamju after saccharification exhibited more activity than simultaneous saccharification in DPPH assay. Conclusion : These results demonstrated that samples of added to the H. rhamnoides L. leaf could be use as functional food.

Background: The flowering plant Hippophae rhamnoides L. has been used for many studies on fruit or leaf extracts. This study was conducted to investigate the development of a new cosmetic material from H. rhamnoides fruits and leaves that have by antioxidant, anti-inflammatory and wrinkle improvement activities. Methods and Results: The antioxidant abilities of H. rhamnoides extracts, including of a water-soluble fruit powder (FW), a fatsoluble fruit powder (FF), a supercritical extract of fruit by-product (BS), and a mixture of leaf and fruit (MIX), were investigated in vitro. A DPPH radical assay for antioxidant activity was performed for these fractions alongside assay to evaluate the total phenolic and flavonoid content (TPC and TFC). As expected, the MIX had the highest DPPH radical scavenging activity (RC50 = 10.27㎍/㎖), and the TPC and TFC also were highest in MIX (225.7 ㎎·GAE/g, and 25.18 ㎎·QE/g, respectively). Nitric oxide (NO) production in LPS-induced RAW264.7 cells was estimated and the results indicated an over 75% decrease of NO production in FF and MIX. In other assays, the highest elastase inhibitory activity was found in FW. Conclusions: These results revealed that H. rhamnoides extracts have a high potential for antioxidant, anti-inflammatory and antiwrinkle activities. H. rhamnoides products are suggested to be applied as the functional materials of cosmetic ingredients.

Background : Sea buckthorn (Hippophae rhamnoides L.) was used as medicinal plant in Tibetan and Mongolian traditional medicines. It has been recognised as a versatile nutraceutical crop with diverse uses for the treatment of diseases, such as gastric ulcers, lung disorders, cardiovascular diseases, mucosal injuries and skin disorders. Physiological research on mixture of sea buckthorn leaf and fruit have not be reported. Therefore, in this study, using sea buckthorn mixture, antioxidant and anti-inflammatory effects were determined. Methods and Results : The experiment was carried out using 11 samples (100% leaf extract - 100% fruit juice powder). The antioxidant and free radical scavenging activities of sea buckthorn mixture were evaluated using DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. The leaf extract with fruit juice in the ratio of 60 : 40 (w/w) showed a significant effect (86.43%). The mixture of sea buckthorn leaf and fruit were investigated for anti-inflammatory activity using LPS stimulated Raw 264.7 cells. The results showed that the higher ratio of leaf extract indicated greater anti-inflammatory activity (approximately 10%, NO production ). Conclusion : These result showed that the mixture of sea buckthorn leaf and fruit can be used as a variety of antioxidant and other functional product research and development processes as valuable natural materials.

In the present study, we investigated the effect of 70% EtOH extract from Hippophae Rhamnoides L. leaves (HRL) on the anti-obesity effect in 3T3-L1 cells. The effects of HRL on lipid accumulation in 3T3-L1 cells were examined using Oil Red O staining. In addition, we examined the gene expression levels by using RT-PCR and western blot. The results of this analysis showed that 100 ㎍/㎖ HRL significantly increased the inhibition of lipid accumulation by 82.25%; significantly decreased the mRNA expression of sterol regulatory element binding protein-1c (SREBP-1c), peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer binding proteins α (C/EBPα), and fatty acid synthase (FAS) in 3T3-L1 cells as well as the stimulated protein expression of AMP-activated protein kinase (AMPK); and suppressed the expression level of PPARγ. These results suggest that HRL can prevent adipogenesis through activation of AMPKα and inhibition of adipogenesis transcription factors.