스트레스 음파(5,000 Hz, 95 dB)는 아메리카잎굴파리(Liriomyza trifolii) 번데기의 성충 발육을 억제시켰다. 이러한 발육 교란에 대한 생리적 원인을 규명하고자 이 곤충의 스트레스 음파에 대한 발현 유전체를 무처리 곤충과 비교 분석하였다. 발현유전체는 전사체를 대상으로 하였으며, 이는 전체 RNA를 Illumina HiSeq2000을 이용한 차세대우전체 염기서열 분석 기술을 이용하였다. 처리구와 무처리구에서 전체 전사체의 분석염기수는 약 16 Gb였고, 이를 토대로 77,553개의 contig가 규명되었다. 이 contig들을 대상으로 스트레스 음파 처리에 따라 발현량 차이를 보인 비율은 약 62.8%를 차지하였다. 이 가운데 대조구에 비해 10배 이상 발현량이 감소한 contig 숫자는 3,994개이고, 증가한 contig 숫자는 1,120개를 나타냈다. 특별히 성장과 변태와 관련된 insulin 및 ecdysone 신호 유전자의 발현이 영향을 받았다. 또한 일반 물질대사와 관련된 유전자들의 발현이 스트레스 음파 처리에 따라 감소를 보였다. 그러나 스트레스 관련 유전자인 지질동원호르몬 신호전달과정 유전자들은 발현량이 증가하였다. 이상의 스트레스 음파에 따라 아메리카잎굴파리의 유전체 발현량 변화는 이 곤충의 정상적 성충 발육에 교란을 주었을 것으로 추정된다. 향후 스트레스 음파에 처리에 따라 발현에 교란을 받은 유전자들의 성충 발육 연관성에 대한 기능 분석이 필요하다.

중요 시설해충인 아메리카잎굴파리(Liriomyza trifolii (Burgess))의 개체군 밀도변동모형을 방울토마토 온실내 대기온도와 잎 표면온도를 이용하여 모형 정확성을 비교하였다. 모형 개발에 이용된 생물적 변수들은 기존 발표된 자료들을 사용하였고 모형 작성은 DYMEX 프로그램을 이용하였다. 온도에 따라 상이한 발육기간과 산란수는 생리적 연령으로 표준화시킨 발육완료 분포모형, 연령 특이적 산란수 및 생존율을 비선형회귀 모형에 적합시켜 밀도변동 모형을 개발하였다. 줄내림방식의 방울토마토에서 식물체를 3개의 위치(상단: 지상 1.6 m 이상, 중단: 지상 0.9 - 1.2 m 사이, 하단: 지상 0.3 - 0.5 m 사이)로 나누고 각 위치별로 온실 내 대기 온도와 잎 표면 온도를 기록하였다. 온실 내 잎 표면 최대온도는 대기중 최대온도보다 항상 낮게 유지되고 있었으며, 하단, 상단, 중단의 순으로 온도가 낮아지는 경향을 보였다. 개발된 모형검정을 위한 초기이입 시기와 밀도는 6월초 성충 5마리가 총 50개의 알을 잎에 산란한 것으로 설정하였다. 온실 내 대기 온도와 잎 표면 온도를 이용하여 아메리카잎굴파리 유충 발육모형과 성충의 산란모형을 DYMEX로 프로그래밍하고 모의실험을 하였다. 모의실험결과를 평가하기 위해 기상자료를 수집한 동일한 온실에서 아메리카잎굴파리 유충 밀도를 육안조사 하였으나, 알, 번데기, 성충의 경우 육안조사가 어려워 대상에서 제외하였다. 육안조사결과 밀도변동패턴이 방울토마토 잎 표면 온도를 이용한 모의실험결과 밀도변동패턴과 유사하였다. 육안조사결과와 육안조사시기의 DYMEX모의실험 결과값을 상관분석 한 결과, 육안조사결과와 잎 표면 온도를 이용한 모의실험 결과가 유의한 양의 상관관계를 보였다(r = 0.97, p < 0.01). 대기 온도를 이용한 모의실험 결과와는 유의하지 않은 상관관계를 보였다(r = 0.40, p = 0.18). 본 연구결과 방울토마토 온실에서 아메리카잎굴파리 개체군 밀도변동의 적절한 예측을 위해서는 잎 표면 온도를 고려해야 하는 것으로 나타났다.

Population dynamics of American serpentine leafminer, Liriomyza trifolii, were modeled and simulated to compare the temperature effects of air and tomato leaf inside greenhouse using DYMEX model simulator. The DYMEX model simulator consisted of series of modules with the parameters of temperature dependent development and oviposition model of L. trifolii from pre-published data. Leaf surface temperature of cherry tomato leafs was monitored according to three tomato plant positions using an infrared temperature gun. Air temperature was monitored at same three positions using a self-contained temperature logger. Data sets of observed air temperature and average leaf surface temperatures were prepared, and incorporated into DYMEX simulator to compare the effects of air and leaf surface temperature on population dynamics of L. trifolii. The number of L. trifolii larvae was counted by visual inspection in tomato plants to verify the performance of DYMEX simulation. The egg, pupa, and adult stage of L. trifolii were not counted due to its availability of visual inspection. Based on correlation analysis, L. trifolii was affected greatly by the leaf temperatures, rather than air temperatures.

This study investigated the adverse effects of sound treatment on physiological processes of the American leafminer, Liriomyza trifolii, during several developmental stages. Larval feeding activity was analyzed by measuring feeding tunnel length. It was significantly suppressed by sound treatment (5,000 Hz, 95 dB). Sound treatment delayed the pupal period at 315 - 5,000 Hz and prevented adult emergence at 1,000 - 5,000 Hz. Female oviposition was also inhibited by the stress sound treatments. However, phototactic adult movement was not affected by sound treatment. Pupae treated with 5,000 Hz showed marked changes in protein pattems analyzed by two dimensional electrophoresis. MALDI-TOF analysis of specific protein spots indicated that trafficking protein particle complex I, triosephosphate isomerase, hypothetical protein TcasGA2_TC013388, polycystin-2, paraneoplastic neuronal antigen MAl, and tropomyosin I (isoform M) were predicted in the control insects and disappeared in the insects treated with sound. By contrast, DOCK9, cytoskeletal keratin II, and F0F1-ATP synthase beta subunit were predicted only in the sound-treated insects. Furthermore, stress sound significantly increased the susceptibility of L. trifolii to insecticides. These results suggest that physiological processes of L. trifolii are altered by sound stress, which may be exploited to develop a novel physical control tactic against L. trifolii.

파리목 곤충의 청각기관은 더듬이의 존스턴 기관으로 알려져 있어, 소리에 대한 아메리카잎굴파리 더듬이의 반응을 EAG를 이용하여 조사하였다. 순음(사인파)과 아메리카잎굴파리 및 다른 3종의 구애노래를 절제된 아메리카잎굴파리 머리에 노 출시키고 EAG를 통하여 반응을 관찰하였다. 순음의 경우 20 Hz의 저주파에서만 EAG 반응이 관찰되었다. 구애노래의 경우 검정발노랑굴파리(Cryptonevra inquilina) 와 일본의 Lipara japonica 구애노래에 대한 반응이 나타났으나 아메리카잎굴파 리 수컷의 구애노래에 대한 암컷의 EAG 반응은 나타나지 않았다. 조사된 음파에 대한 반응행동을 관찰하기 위해 2차원 궤적 추적을 추가적으로 실행하였다. 기록 된 궤적은 x축 궤적의 평균값을 비교하여 소리에 대한 유인효과를 조사하였고, 궤 적의 정보 엔트로피를 이용하여 활동성 차이를 분석하였다. x축 평균값은 소리에 대한 차이가 없어, 소리에 대한 아메리카잎굴파리의 유인효과는 관찰하지 못 하였 다. 처녀 수컷과 암컷, 교미한 수컷에서는 소리를 노출시켰을 때 정보 엔트로피 값 이 감소하여 활동성이 차이가 남을 알 수 있었으나 교미한 암컷에서는 활동성의 차이가 없었다.

The American serpentine leafminer fly, Liriomyza trifolii (Burgess), is one of the most destructive polyphagous pests world wide. In this study, we determined electron beam doses for inhibition of normal development of the L. trifolii and investigated the effect of electron beam irradiation on DNA damage and p53 stability. Eggs, larvae, pupae and adults were irradiated with increasing doses of electron beam irradiation (six levels ranges from 30 to 200 Gy). At 150 Gy, the number of adults that developed from irradiated eggs, larvae and pupae was lower than those of untreated control. Fecundity and egg hatchability decreased depending on the doses applied. Reciprocal crosses between irradiated and unirradiated flies demonstrated that males were more radiotolerant than females. Adult longevity was not affected in all stages. The levels of DNA damage in L. trifolii adults were evaluated using the alkaline comet assay. Our results indicate that electron beam irradiation increased levels of DNA damage in a dose-dependent manner. Moreover, low doses of electron beam irradiation led to the rapid appearance of p53 protein with in 6 h; however, it decreased after exposure to high doses (150 Gy and 200 Gy). These results suggest that electron beam irradiation induced not only abnormal development and reproduction but also p53 stability caused by DNA damage in L. trifolii. We conclude that a minimum dose of 150 Gy should be sufficient for sterilization of L. trifolii.

The EAG (Electroantennogram) has been used to find out chemical substance effective to insect. We tried to use EAG recording for catching of responsible acoustic signals to Lirimyza trifolii (Diptera: Agromizidae). In fly auditory organ known as Johnston's organ located on antenna. Pure tone sounds (sine wave) and courtship songs of several kinds of species were broadcasted to excised head of the fly. Significant EAG signals were detected when released the 20 Hz pure tone sound and long pulse train patterns, which were courtship songs of Cryptonevra inquilina and Lipara japonica (Chloropidae). For conformation of it's effectiveness, we further analyzed movement behavior with the signal got from the EAG recording. Information entropy could represent the specimens activities of movement tracks. The entropy with sound was relatively lower than without sound. The combination of EAG responding and behavioral observation would be useful to analyze the response of insect pest against acoustic signals and develop acoustic pest managing tool in agriculture.

Occurrence of Liriomyza trifolii and its biological control efficacy using Neochrysocharis formosa were evaluated in two eggplant cropping systems of spring and autumn cultivation. L. trifolii adults began to be attracted on a yellow sticky traps from late April and they increased from early June. A high density of L. trifolii adults was maintained from middle June to middle July. The releases of two N. formosa per plant with 3 times as weekly intervals from May 25, 2004 for spring culture resulted control effect of 90.1% in parasitism to L. trifolii in late July. The releases of two N. formosa per plant with 4 times as weekly intervals from Auguest 31, 2004 for autumn culture resulted control effect of 81.3% in population of L. trifolii with 64.4-69.9% in parasitism.

Economic injury levels and economic thresholds were estimated for the american serpentine leafminer (Liriomyza trifolii) on greenhouse eggplant. Liriomyza trifolii density was increased until the late June and decreased after the July in innoculation on may 17. Growth of an aerial plants and fruits were not different in treatment respectively. But total number of fruits and yields were decreased on higher inoculation density. Whereas the rate of yield loss was increased. The rates of damaged leaf by L. trifolii were increased on higher inoculation density and the peak was 65%. The number of commodity fruits and the rates of commodity fruit were become lower than non-treatment (72.2%). The rates of damaged leaf area were 5.3, 11.7, 19.7, 25.7% on inoculation densities and the rates of yield loss were 0.6, 4.8, 9.8, 14.7%, respectively. There existed close correlation between rate of yield loss and inoculation density (Y = 0.76779X + 0.298354, R² = 0.9599). Considerated of the results, the economic injury levels of L. trifolii on eggplant greenhouse was 6.1 adults per 4 plant and the economic thresholds was 4.9 adults per 4 plant

Biological control of Liriomyza trifolii (Burgess) using Diglyphus isaea (Walker) has been evaluated in tomato greenhouse, for three seasonal culture types: spring type (March-July), summer type (June-October) and autumn type (July-December). For spring type, totally 5.8 individuals/㎡ of D. isaea has been released at six times from late April, when the density of L. trifolii was about 1.0 individuals/plant. Corrected mortality of Liriomyza trifolii caused by parasitoids was 97.6% at early July, and the proportion of D. isaea was 88.9% of all parasitoids collected in the greenhouse. In the case of summer type, totally 1.8 individuals/㎡ of D. isaea has been released at five times from early July, when the density of L. trifolii was about 0.4 individuals/plant. Corrected mortality of L. trifolii caused by parasitoids was 84.4% during the whole season, but the proportion of D. isaea was very low (only 13.8%). Immigrating parasitoids such as Chrysocharis penthus were synchronized to control the leafminer in the greenhouse. For autumn type, totally 2.7 individuals/㎡ of D. isaea has been released at four times from mid September, when the density of L. trifolii was about 0.7 individuals/plant. Corrected mortality of L. trifolii caused by parasitoids was 85.7% at mid December, and the proportion of the D. isaea was 83.4%.

Experiments have been performed to investigate biological characteristics of Diglyphus isaea (Walker), an ectoparasite of Liriomyza trifolii (Burgess). At 25℃, development time of D. isaea was estimated as 1.3 days for eggs, 4.5 days for larvae and 6.3 days for pupae. Adult longevity was 25.1 days, and a female adult could lay 305.3 eggs throughout its lifetime. Number of L. trifolii parasitized or killed by the wasp was 962.2 individuals/adult. Most of all parasitized or killed larvae were identified as third instar. With 20% honeyed water as food source, half of the adults could survive 180 days at 5℃, 150 days at 10℃, and 90 days at 15℃. After storing at 10℃ with absolute honey for 30 days, 70 days and 140 days, a female adult in room temperature laid eggs 190.8 individuals, 104.0 individuals and 89.4 individuals, respectively.

The antennal receptors of the adult male and female of the American leafminer, Liriomyza trifolii (Diptera: Agromyzidae) was studied using the scanning electron microscopy. This antennal receptors were developed to accept the chemical or mechanical signal in the environment. The antennae of L. trifolii is 317.52 ㎛ long and composed of the scape, pedicel, funicle, arista. Antennae located between compounds eye on the middle in the head. The result of SEM observation, four types of receptors were characterized and grouped into morphological classes: Trichoid, Basiconic, Coeloconic, Chetiform sensilla. Trichoid sensilla are located on the all segment. As a rule, female is more than male. This sensilla are long and slender hair with straight or slightly curved, and taper to a sharp point apically. Basiconic sensilla are observed only on the funicle. The number of basiconic sensilla in male and female are almost same in both sexes. This sensilla are long and thick hair with stright or slightly curved, and taper to a blunt point apically. Coeloconic sensilla are observed only on the funicle. This sensilla were showed a finger-like projections the peg apex. The number of basiconic sensilla in male and female are almost same in both sexes. Chetiform sensilla are located on the all segment. This sensilla are needle-like, tapering sharply. Pedicel are more than scape. The number of Chetiform sensilla in male and female are almost same in both sexes.

아메리카잎굴파리의 내부기생봉인 Neochrysocharis formosa의 산란과 기주체액섭취 선호성을 조사하였다. N. formosa는 기주유충의 몸 뒤쪽에 대부분 산란하였다. 온도 조건에 따른 일산란수와 일기주체액섭취수를 조사한 결과 온도가 높을수록 증가하는 경향이었으며 15, 20, 25, 3에서의 산란수와 체액섭취수의 비율은 각각 0.5, 0.9, 1.0, 1.0이었다. 항온조건하에서 N. formosa의 총산란수와 기주체액섭취수는 각각 176개 188.7개이었으며 산란수와 기주체액섭취수 비율은 0.9이었고 암컷 수명은 11.8일이었다. 산란과 기주체액섭취는 주로 아메리카잎굴파리 3령유층을 선호하였다.