Biocide dichlofluanid breaks down quickly and accumulates easily in sediment, potentially causing a persistent impact on various marine organisms. We analyzed the potential toxicity of dichlofluanid on major aquaculture species in Korea, Undaria pinnatifida. Female gametophytes of U. pinnatifida were exposed to dichlofluanid at concentrations of 0, 1, 2, 4, 8, 16, and 32 mg L-1, and their survival and relative growth rate were analyzed. The no observed effect concentration (NOEC), lowest observed effect concentration (LOEC), and median lethal concentration (LC50) for female gametophyte survival were determined as 1, 2, and 10.82 (95% CI: 8.87-13.23) mg L-1, respectively. The NOEC, LOEC, and median effective concentration (EC50) for relative growth rate were 1, 2, and 6.58 (95% CI: 6.03-7.17) mg L-1, respectively. Female gametophytes of U. pinnatifida were expected to experience toxic effects at concentrations above 2 mg L-1 of dichlofluanid. These research findings are expected to serve as important reference data for evaluating the toxicity effects of U. pinnatifida in its early life stages when exposed to dichlofluanid.

Chlorothalonil is continuously introduced into the marine environment and has significant toxic effects on various marine organisms, however, research on its effect on seaweed is limited. Therefore, we analyzed the impact of chlorothalonil on the early life stages of major aquaculture species in Korea, Undaria pinnatifida. U. pinnatifida female gametophytes were exposed to different concentrations of chlorothalonil (0, 0.03, 0.05, 0.10, 0.20, and 0.40 mg L-1), and their survival rate and relative growth rate were analyzed. The no observed effect concentration (NOEC), lowest observed effect concentration (LOEC), and median lethal concentration (LC50) for female gametophyte survival were determined as 0.05, 0.10, and 0.141 (0.121-0.166) mg L-1. NOEC, LOEC, and median effective concentration (EC50) for relative growth rate were 0.10, 0.20, and 0.124 (0.119-0.131) mg L-1. Therefore, female gametophytes of U. pinnatifida are expected to experience toxic effects at concentrations above 0.05-0.10 mg L-1 of chlorothalonil. These research findings are anticipated to serve as crucial reference data for evaluating the effects of chlorothalonil on the health of U. pinnatifida in the early life stages.

미역(Undaria pinnatifida) 포자를 사용한 생태독성 시험법은 연중 상시 이용하기에 어렵다. 미역 의 암배우체는 실험실에서 배양할 수 있기 때문에, 포자의 단점을 보완하고 상시 생태독성 시 험에 사용할 수 있다. 따라서 미역의 암배우체를 생태독성 시험에 활용하기 위해 다양한 환경 조건에 노출된 미역의 암배우체 생존율과 상대성장률의 변화를 분석하였다. 미역 암배우체는 염분(5~40 psu), 온도(5~30℃), pH (2~10), 광량(0~120 μmol photon m-2 s-1)에 노출되었다. 암배 우체의 생존율은 최고 평균값을 기준으로 온도 20℃, 염분 27.5 psu, pH 8, 광량 30 μmol photon m-2 s-1에서 가장 높았다. 상대성장률은 최고 평균값을 기준으로 온도 15℃, 염문 35 psu, pH 9, 광량 60 μmol photon m-2 s-1에서 가장 높았다. 본 연구결과를 통해 미역 암배우체의 생존율과 상대성장률을 활용하여 연안환경을 상시 신속하게 평가하기 위한 효율적인 방법으로 활용이 기대된다.

표고 시설재배에 있어 적정 상대습도를 구명하여 고품질 버섯생산 데이터를 제시하기 위해 상대습도를 달리 처리한 결과는 다음과 같다. 상대습도에 따른 생육특성을 조사한 결과, 갓의 명도값은 RH65%에서 높았고, 적색도는 RH95%에서 높았으며, 황색도는 상대습도 간 유의적 차이가 나타나지 않았다. 갓의 경도는 RH95%에서 높은 경향이었다. 갓직경은 RH95%에서 높게 측정되었고, 갓두께는 RH95%에서 높았다. 대길이의 경우 유의적 차이가 나타나지 않았지만 상대습도가 증가할수록 대길이가 증가하는 경향을 보였으며, 대직경은 RH95%에서 높게 측정되었다. 수량은 RH95%에서 유의적으로 높게 측정되었다. 화고발생률은 상대습도가 낮아질수록 높게 조사되었다. 위의 결과를 통해 RH95%에서 표고 자실체의 크기, 수량이 높게 조사되었지만 버섯의 갓색이 어둡고 화고 발생 비율이 낮은 결과를 나타내어 시설재배에서 고품질의 화고 버섯을 생산 할 수 있는 상대습도는 65%와 85%인 것으로 판단되었다.

3종의 일반토마토 ‘대프니스’, ‘도태랑다이아’, ‘마스카라’를 대목 ‘B블로킹’에 접목하고 접목 활착 후 10일부터 10일 간격으로 40일 까지 육묘용 표준양액(S)의 0.5S, 1,0S, 2,0S 로 시비수준 처리를 하였다. 각 시기별로 묘의 생육 인자들을 조사하였고, 묘소질 지표인 TR율, 충실도, DQI를 계산하였다. 그리고 생육인자들과 묘소질 지표와의 상관관계를 분석하였고, 묘령별로 야간온도를 10, 15, 25℃를 목표로 3수준으로 처리하여 상대생장률(RGR)을 분석하였다. 그 결과, 토마토 접목묘의 양적인 생육인자들은 모두 시비농도가 높을수록 증가하였고, 다양한 크기 범위의 묘를 생산할 수 있었다. 그리고 묘의 바이오메스 관련 생육인자가 충실도와 DQI에 높은 정의상관이 있음을 알 수 있었으며, 충실도와 DQI의 고도로 유의한 1차 선형회귀식((Y=-0.0256+0.0029*X, Adj R2=0.9480, p<0.0001)이 도출되었다. 정식 후 2주간의 RGR은 묘령이 높아질수록 감소하는 경향을 보였고, 육묘기 시비수준에 따라서는 접목활착 후 30일묘와 40일묘에서 표준 시비수준 1.0S에서 RGR이 높았다. 시비수준별 육묘기 DQI와 정식후 초기 RGR과 유의한 선형회귀식을 도출하였으며, 표준 시비수준 1.0S에서 DQI가 높아짐에 따라 RGR이 높게 유지되었다. 이러한 결과는 DQI가 원통형 종이포트 토마토 접목묘의 묘소질의 지표로 이용될 수 있고, DQI와 정식 후 초기 상대생장률과의 관계분석을 통하여 우량묘의 기준 설정에 활용될 수 있을 것이다.

This study is investigated the growth characteristics(number of available stipe, pileus diameter, pileus thickness, stipe length, stipe thickness, object weight, comparison yield ability of 1 cycle) and storage characteristics of ‘Sanjo 701’ (S7) cultivars according to relative humidity. The S7 growth characteristics were investigated by quantifying the growth and the characteristics according to the relative humidity, The storage stability was investigated every 5 days and freshness was measured by ‘Minamide Method’. S7 pileus diameter is The higher the relative humidity was confirmed becomes larger and the more increased relative humidity also increase the comparison yield ability of 1 cycle. However, pileus diameter or stipe length This could not see the big difference in the three treatment groups, the plieus thickness was no significant difference in the treatment of 80% and 95%. The fresh weight of S7 decreased significantly at 80% and 95% relative humidity after 10 days of storage, but decreased continuity in 65% humiditiry. The elongation percentage of S7 pileus was observe in 95% relative humidity, values of L, a and b (SCI), showed the highest L value in 65% and the a value in 95%. b values were similar in 65%, 80% and 95% treatments. On the ‘Minamide Method’ measure freshness was changed from the 10th day of storage at relative humidity of 95%, but humidity of 65% and 80% treatments, it changed after 15 days of storage. In this study growth characteristics and yield were increased at higher relative humidity, but storage stability was decreased. Therefore, it is deemed necessary to change the relative humidity to produce high quality mushrooms.

본 연구는 식물공장에서 미나리 재배시 명/암주기와 암기시간 동안의 상대습도 조절이 미나리의 생육과 품질에 미치는 영향을 알아보고자 수행하였다. 정식 후 30일째에 수확하여 생육 및 품질을 비교하였을 때 상대습도 60/ 90%(명/암기) 처리가 60/60% 처리보다 지상부 생체중이 뚜렷이 증가하였다. 상대습도60/60% 처리에서 명기가 연장될수록 지상부 생체중이 증가하였지만 60/90% 처리에서는 16/8h 처리에서 지상부 생체중이 가장 높았으며, 20/ 4h, 22/2h 순이었다. 팁번의 발생은 상대습도 60/90%에서 는 명/암주기 16/8h로 처리했을 때 1.4%로 매우 낮았으나, 명기 시간이 연장된20/4h, 22/2h 처리에서 각각 15.5% 30.3%로 급격히 증가하였다. 상대습도 60/60%에서는 명/ 암주기 16/8h에서도 팁번의 발생이 15.5%로 높았으며 20/ 4h, 22/2h 처리에서는 25% 이상 높게 나왔다. 줄기의 경도는 상대습도 60/90%, 명/암주기 16/8h 처리에서 가장 낮았다. 잎의 무기성분은 명기시간이 연장될수록 감소하였지만Ca의 함량은 상대습도 60/60%, 명/암주기 22/2h 처리를 제외하고는 유의한 차이가 없었다.

This research was relative humidity (65, 75, 85 and 95%) growth characteristics of the oak mushroom (Lentinula edodes) 'Nongjingo’. At the relative humidity was 65% in growth chamber. On this condition the moisture content of medium surface was 60.5%. That was lower than other treatments. That condition makes Pileus was 77.1% and stipe was 65%. Among the growth characteristics according to the relative humidity, the pileus diameter at 75% relative humidity were 45.5mm that largest than other treatment and pileus thickness at 75% relative humidity were largest than other treatment. The stipe thickness at 65% relative humidity were 14.8mm that lagest than other treatment. The number of available stipes at 95% relative humidity were 13.3peace/2kg that largest than other treatment. The comparison yield ability of 1 cycle was 104.2g that result in largest than other treatment at 95% relative humidity. This result was associated to the relative humidity of fruiting bodies, the lower relative humidity result in the lower of yield. However, the lower relative humidity result in the higher number of whago, Especially, at the treatment of 65% relative humidity, all the fruit bodies were produced as Whago (dry). Therefore, the higher relative humidity influenced in the higher yield but produced water mushroom. That result was thought that ‘Nongjingo’ would be possible to produce high quality mushroom by adjusting the relative humidity appropriately.

접목 후 활착환경의 관리는 접목의 성공여부를 결정하는 중요한 요인이다. 일반적으로 활착초기 접목부위의 캘러스 분화를 촉진하고 식물체의 지나친 위조를 막기 위해 25~30℃ 정도 온도와 90% 이상의 높음상대습도 조건하에서 관리하다가 활착이 진행됨에 따라 점차 온도 및 상대습도를 낮추고, 광 조사량을 늘려주는 환경관리방법이 제시되고 있다(Kim 등, 2001). 본 연구에서는 고추 접목묘의 활착 및 생육향상을 위한 활착단계별 환경조건을 제시하고자, 활착기간 중 온도, 상대습도 및 광 조건이 접목활착률 및 생육에 미치는 영향을 조사하였다. 암조건 하에서 총 6일간의 접목활착기간을 3단계로 나누어, 활착 1단계는 온도 30℃, 상대습도 95% 조건하에서, 활착 2, 3단계에서는 온도(20℃, 25℃, 및 30℃) 및 상대습도(75%, 85%, 및 95%) 조건을 달리 처리하였을 때, 접목활착률 및 생육을 조사하였다. 고추 접목묘의 활착을 위해서는 활착 초기 30℃, 95% 상대습도 조건에서 2일정도 관리한 후, 이후 4일간은 온도와 상대습도 조건을 각각 20~25℃, 75~85% 정도로 낮추어 주는 것이 바람직할 것으로 판단된다. 또한 접목활착기간 동안 온도 30℃, 상대습도 85% 및 암 조건을 대조구로 하여, 활착기간 중 온도(25℃ 및 30℃), 상대습도(65%, 75%, 및 85%) 및 광 조건(광 조사 유무, 광 조건 45±2μmol·m-2·s-1)을 달리하여 처리하였 때, 접목 활착률은 처리간 차이가 없었으며 활착기간 중 저온 저습의 광조사 기간이 길수록 생육이 증가하는 경향을 보여, 25℃ 온도조건하에서 저광 조사 및 65% 까지의 저습 조건이 고추 접목묘의 활착 및 생육촉진에 효과적인 것으로 판단된다.

This study was conducted to analyze the variations of air temperature, relative humidity and pressure in a low pressure chamber for plant growth. The low pressure chamber was composed of an acrylic cylinder, a stainless plate, a mass flow controller, an elastomer pressure controller, a read-out-box, a vacuum pump, and sensors of air temperature, relative humidity, and pressure. The pressure leakage in the low pressure chamber was greatly affected by the material and connection method of tubes. The leakage rate in the low pressure chamber with the welding of the stainless tubes and a plate decreased by 0.21kPa·h-1, whereas the leakage in the low pressure chamber with teflon tube and rubber O-ring was given by 1.03kPa·h-1. Pressure in the low pressure chamber was sensitively fluctuated by the air temperature inside the chamber. An elastomer pressure controller was installed to keep the pressure in the low pressure chamber at a setting value. However, inside relative humidity at dark period increased to saturation level.. Two levels (25 and 50kPa) of pressure and two levels (500 and 1,000sccm) of mass flow rate were provided to investigate the effect of low pressure and mass flow rate on relative humidity inside the chamber. It was concluded that low setting value of pressure and high mass flow rate of mixed gas were the effective methods to control the pressure and to suppress the excessive rise of relative humidity inside the chamber.

The Diameter at Breast Height (DBH) provides information about the volume growth of a tree. In this study, we estimated the relative growth rates of Castanea crenata and Pinus rigida as 4.07% and 3.73%, respectively. Although the difference was low, we demonstrated that the growth rate of C. crenata is slightly faster than that of P. rigida. After calculating the relative growth rate for each section, we found that the relative growth of C. crenata decreased with time. However, the relative growth rate of P. rigida showed an overall increase. The gap widths of both species showed an increasing trend. However, the gradient of the two species was different. The gradient of C. crenata was approximately 12.0, but that of P. rigida was approximately 4.7. This means that the volume growth of C. crenata was faster than that of P. rigida during 4 years. However, this was relatively a short period for measuring the volume growth pattern, and we believe that additional useful information can be obtained by conducting long-term ecological monitoring. Results of canonical correspondence analysis showed that among the climate variables, temperature was significantly related to the gap widths for both species.

This study was carried out to produce more Parasenecio firmus in forest farming. In order to achieve thispurpose, it was surveyed the growth and photosynthetic characteristics of P. firmus. Relatively light intensity was controlledby 100%, 60%, 30% and 5% of full sunlight. Height was the highest under 5% of full sunlight. Shoot diameter was the high-est in full sunlight. Fresh weight (leaf, stem, root and total) and dry weight (leaf, root and total) were the highest under 30%of full sunlight. S (leaf+stem)/R (root) ratio was the lowest under 30% of full sunlight and the highest under 5% of full sun-light. In leaf characteristics, leaf area, SLA and LAR were getting higher in the lower light level and the highest under 5% offull sunlight (176.1㎠, 420.5㎠•g−1 and 123.5㎠•g−1). Especially, leaf area was surveyed higher under 30% of full sunlightin the next. Leaf thickness was getting lower in the lower light level and the lowest under 5% of full sunlight (overall0.14~0.24㎜). As a result of surveying the whole experiment, P. firmus grows well under 30% and 5% of full sunlight inforest farming.

This study was carried out in order to investigate the early growth and root development characteristics ofAtractylodes japonica container seedling. Experiment was performed by light intensity (100%, 60%, 25% and 10% of fullsunlight). Height was surveyed well under 25% of full sunlight (overall 11.013.0%). Root collar diameter was the highest inthe full sunlight (7.52㎜). It was the highest fresh weight (1.52g, 0.92g, 0.90g and 3.34g) under 25% of full sunlight, rootdry weight was getting higher in the higher light level and the highest in the full sunlight (0.13g). Total root length, rootproject area, root surface area and root volume were the highest under 25% of full sunlight (434.7㎝, 17.4㎠, 54.7㎠,0.55㎤, respectively) and the lowest under 10% of full sunlight. As a result of surveying the whole experiment, A. japonica isjudged nicely by maintaining 25% of full sunlight, excepting 10% of full sunlight. And light levels during the early growthare overly important to improve early growth.

The purpose of this study is to compare the relative growth of annual ring width of red pine(Pinus densiflora), black pine(Pinus thunbergii) and pitch pine(Pinus rigida) by means of multiple regression method according to Graybill hypothesis. The obtained results are as follows. 1. The changes of rainfall have affected to tree growth during the periods of 1975 through 1978. 2. Among these pine trees, red pine was mostly influenced by environmental factors. 3. The growth of annual ring width was sensitively responded to the changes of rainfall and air temperature. 4. Among the heavy metals analyzed, the concentrations(ppm) of Lead(Pb) and Copper(Cu) were negatively effected on the growth of annual ring width of pine trees. 5. The analytical technique of annual ring width may be useful for estimation of the pollution in forest areas near industrial complexes.

소나무 수형목 풍매차대를 대상으로 수고생장에 대하여 일반조합능력을 평가하고자 본 연구를 수행하였다. 본 연구에 사용된 가계는 총 232가계로 각 수령별 10년생 230가계, 20년생 184가계, 30년생 122가계를 대상으로, 차대검정림에서의 수고 생장 조사 자료를 이용하였다. 차대검정림은 1975년부터 조성하기 시작하여 1987년까지 모두 7회에 걸쳐 1~4 지역에 조성하였다. 이러한 조성 년도, 지역의 차이를 표준화를 통하여 균일한 수치로 보정하여