돼지고기 중 5종의 곰팡이독소(아플라톡신 B1, B2, G1, G2 및 오크라톡신 A)를 분석하기 위해 0.1% 개미산을 함유한 50% 아세토니트릴용액으로 추출한 후 고체상추출칼럼을 이용하여 정제하고, LC-MS/MS로 동시 정량할 수 있는 시험법을 마련하였다. 분석조건으로 측정한 5종의 곰팡이독소 matrix-matched 표준곡선식에서 모두 상관계수 0.998이상의 상관관계를 나타내었다. 5종의 곰팡이독소 2배의 정량한계에서 10배의 정량한계로 첨가한 시료에서 평균 회수율은 72.1~109.9%로 실험 결과들이 EU 가이드라인에서 제시하는 유효성 확인을 위한 기준을 만족함으로써 시험법의 신뢰성을 확보할 수 있었다. 충청지역 유통되고 있은 돼지고기 20건에 대한 총아플라톡신 및 오크라톡신A에 대한 오염도 조사결과 정량한계 미만으로 조사되었다.

HPLC-MS/MS를 이용하여 벌꿀의 동물용의약품 동시분석을 위한 분석법을 정립하고자 식품공전 중 벌꿀에 규격이 설정된 11종의 동물용의약품을 2개 그룹 Group 1 (streptomycine, dihydrostreptomycine, neomycine)과 Group 2 (oxytetracycline, enrofloxacin, ciprofloxacin, cymiazole, chloramphenicol, amitraz, coumaphos, fluvalinate)으로 나눠서 동시에 분석 할 수 있는 방법을 연구 하였다. 두 그룹모두 RT는 15분 이내였고, 검출한계는 0.0056~0.0643 μg/g, 정량한계는 0.0169~0.1948 μg/g으로 나타났으며 Group 1 (0.05~1.0 μg/g의 농도범위)과 Group 2 (0.01~1.0 μg/mL) 의 검량선을 작성한 결과 각 동물용의약품의 직선상관계수(R2)는 0.9917~0.9987, 0.9923~1.000으로 매우 양호한 상태를 보였고, 최종 농도가 0.25 μg/g, 1.0 μg/g에서의 회수율은 Group 1 65.1~80.6%, Group 2 64.2~90.3%를 나타났다, 또한 area 및 RT 에 대한 inter (n = 3), intra day (n = 6) RSD (%) 분석결과는 area는 10.92%이하, RT은 1.57% 이하로 나타나 양호한 수준을 보여 벌꿀 중 동물용의약품동시분석 방법으로 적합하다고 판단된다.

We observe ten known 22GHz H2O maser galaxies during February 19–22, 2011 using the 21 m Tamna telescope of the Korean VLBI Network and a new wide-band digital spectrometer. Simultaneously we searched for 43GHz SiO v = 1, 2, J = 1–0 maser emission. We detect H2O maser emission towards five sources (M 33, NGC 1052, NGC 1068, NGC 4258, M 82), with non-detections towards the remaining sources (UGC 3193, UGC 3789, Antennae H2O-West, M 51, NGC 6323) likely due to sensitivity. Our 22GHz spectra are consistent with earlier findings. Our simultaneous 43GHz SiO maser search produced non-detections, yielding – for the first time – upper limits on the 43GHz SiO maser emission in these sources at a 3  sensitivity level of 0.018K–0.033K (0.24 Jy–0.44 Jy) in a 1.75 km s−1 velocity resolution. Our findings suggest that any 43GHz SiO masers in these sources (some having starburst-associated H2O kilomasers) must be faint compared to the 22GHz H2O maser emission.

We analyzed 0.5-45 keV data of NGC 3227 observed by Suzaku six times between 2008 October 28 and December 2. The count-count plot between the 0.5-3 keV and 3-10 keV bands exhibits a clear break, separating the data into bright and faint phases. Applying the difference spectrum method and time-averaged spectral fits to the phase data, we found the presence of two kinds of variable primary X-rays, (1) a hard primary component with Γ 1.7 dominating in the faint phase and (2) a soft primary continuum with Γ ~ 2.4 appearing in the bright phase, both affected by partial absorption. Considering their timing and spectral characteristics, component (1) is presumably identical to a Compton continuum in the low/hard state, while component (2) may correspond to the hard tail emission in the high/soft state, or compact-jet emission. In that case, an accretion ow onto the central super massive black hole in NGC 3227 can be interpreted to include the two different states.

Mycotoxins, such as aflatoxin B1 (AFB1), deoxynivalenol (DON), fumonisin B1 (FMB1), ochratoxin A, T2 toxin, and zearalenone, are found in numerous vegetables. Mycotoxin accumulation in food and feed poses serious health risks to humans and animals because of carcinogenic, mutagenic, teratogenic, and toxic properties. In addition, mycotoxins cause large economic losses in commercial crop production, food and feed processing, and animal husbandry worldwide. In this study, an analytical method for the simultaneous analysis of the levels of AFB1, DON, and FMB1 in cow blood with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and validated. AOZTM and Myco6in1TM multitoxin immunoaffinity columns and an OasisTM reversed-phase solid-phase extraction Hydrophilic-Lipophilic-Balanced columns were used to purify and concentrate the blood samples. Extracts that contained AFB1, DON, and FMB1 had average recovery of 64.0%, 98.0%, and 89.9%, respectively. In conclusion, we used LC-MS/MS to detect several important toxicological mycotoxins in cow blood. The multimycotoxin method, which detected and quantified the levels of AFB1, DON, and FMB1 can be used in animal pilot studies to monitor simultaneous exposure to major mycotoxins.

Simultanious quantitative determination of Cetyltrimetyl ammonium chloride(CTM), Stearamidopropyl dimetylamine(SAP), Behentriammonium chloride(BHT), Dihexadeyldimethyl ammonium chloride(DHDDM) was carried out by reversed phase HPLC. The 0.2% TFA/Methanol was used for the mobile phase of gradient conditions. An Alltech C18(250 mm × 4.6 mm i.d., 5 μm)and the selected ELSD detector was applied. The analysis results of HPLC showed goodlinearity with correlation coefficient of r2=0.997 in the rage of 130~1,980.5 ㎍/mL and detection limit.

The experiment was performed using the cleaning precipitator To investigate the absorption efficiency of the SOX/NOX of the aqueous ammonia solution. Concentration of the cleaning liquid is 0.1, 0.5, and 1.0% with increasing absorption efficiency has improved. However, the reaction shown only a difference in time. Absorption efficiency has improved in accordance with the gas residence time. When the direction of the same gas and the cleaning liquid is determined that there is the effect of increasing the residence time. The relative impact of SOX and NOX is this likely to react slower than SOX/NOX. The yield is determined to require

‘Pearl Stars’는 2013년 전라북도농업기술원에서 육성한절화용 백색 겹꽃 안개초 신품종으로 분지각이 작고 줄기가 단단한 특성을 가진다. 2009년 화색이 밝고 소화수가 많은 ‘Gyp04-11’계통을 모본으로 화경장이 길고 줄기가 단단한 ‘Gyp07-39’ 계통을 부본으로 교배하여 78개 종자를 채종하였다. 2010년 파종하여 획득한 71개 실생계통을 특성 평가한 결과 백색 겹꽃으로 일시개화성이며 분지각이 작은 ‘Gyp11-52’을 선발하였고, 3년 동안 특성평가를 실시하여 ‘Pearl Stars’를 육성하였다. ‘PearlStars’의 초형은 직립형이고 잎은 좁은 타원 모양, 소화는 백색 겹꽃에 소화수가 많고 일시개화성이 우수하다. 화서의 외부 위 모양은 둥글고 꽃잎 둥근 정도는 중간, 꽃잎 굴곡은 밖으로 굽은 식물체 고유 형태적 특성을 가진다. 또한 주 화경의 직립정도가 강하고 1차 측지의 분지각이 작으며, 안토시아닌 색소 발현이 많고 흰가루병에대체로 약한 편이다. 익산에서 7월 2일 ‘Pearl Stars’와 대조품종인 ‘Mirabella’ 삽아묘를 정식하여 가을 개화작형에서 특성검정한 결과, ‘Pearl Stars’가 ‘Mirabella’보다 8일 정도 개화가 빨랐고, 마디수가 적고 화경장이 짧았으며, 주당 발생 분지수는 2.2개 정도 더 많았다. 엽장, 꽃자루 길이, 열편크기는 ‘Mirabella’보다 짧았고, 엽폭과 소화크기는 더 컸다. 소화수가 많고 일시 개화성이 우수하고 줄기가 단단하여 시장성이 우수하며, 2015년 3월 국립종자원에 신품종으로 등록(제5356호)되었다.

The purpose of this paper is to investigate the determinants of new product diffusion. We seek to document and explain systematic features of product diffusion. In this essay, we examine the well-documented empirical regularity that the speed of diffusion has accelerated during the twentieth century. The empirical results show that the main source of acceleration are faster declines in prices. Faster price declines make the product affordable to more consumers within a given period of time. Based on theories of intertemporal price discrimination and learning-by-doing, the association between the speed of adoption and the speed of price decline was explained. Faster price declines are attributed to several product characteristics as well as changes in income distribution. Above all, the introduction of consumer electronic products in more recent years can be regarded as the most important factor in accelerating price declines. Consumer electronic products are technologically different from non-electronic goods, in that semiconductors are important components. As the price of semiconductors has dropped rapidly, the falling production costs can be rapidly incorporated to the price of consumer electronic goods. Furthermore, most of the recently introduced consumer electronic products have network externalities, and many products with network externalities require complementary products. A complementary product becomes more readily or cheaply available as more people have the main product. One major difference between previous studies and this study is that the former focuses only on the factors that operate directly on the speed of adoption, while this study incorporated factors that work through price changes as well as the factors that work directly on the speed of adoption.

The experiment was performed for in order to remove NOx which is generated in the Ship's engine. it was performed test in order to remove NOx which is generated in the Ship's engine. It was used as the oxidizing agent sodium chlorite. Use the oxidizer is nitrogen monoxide was oxidized to nitrogen dioxide. and was tested pH adjustment to increase the efficiency of oxidizing. An aqueous solution of sodium hydroxide was used for the nitrogen dioxide absorbent. Low concentration of the solution, it showed a high efficiency. improves the absorption efficiency by add additives.

건강기능식품 등 중 수용성 비타민 B군 (비타민 B1, 비타민 B2, 나이아신, 비타민 B6)의 동시분석 방법을 확립하기 위하여 본 연구를 실시하였다. HPLC에 의한 수용성 비타민 B군의 분석 방법은 기기분석조건을 확립하고, 국내외 시험법 및 논문을 참고하여 시험 데이터를 분석하였다. 수용성 비타민 B군의 HPLC 분석은 270 nm에서 Capcell Pak C18 UG 120 컬럼을 사용하여 진행되었다. 분석법 검증은 수용성 비타민 B군의 직선성, 정확성, 정량한계, 정밀성에 대해 수행되었다. 직선성은 비타민 B1, 비타민 B2,나이아신(nicotinic acid, nicotinamide), 비타민 B6 모두0.1~2 μm/mL의 농도범위에서 R2= 0.999 이상의 우수한 직선성을 확인할 수 있었다. 회수율을 검토한 결과, 비타민 B1 100~103%, 비타민 B2 104~112%, nicotinic acid 82~85%, nicotinamide 121~124%, 비타민 B6 95~104%의 양호한 회수율을 확인할 수 있었다. LOQ는 비타민 B1 0.04μm/mL, 비타민 B2 0.05 μm/mL, nicotinic acid 0.15 μm/ mL, nicotinamide 0.08 μm/mL, 비타민 B6 0.63 μm/mL 이었다. 정밀성을 검토한 결과, B1 0.4%, 비타민 B2 0.4%, nicotinic acid 0.5%, nicotinamide 0.7%, 비타민 B6 0.4% 로 1% 이내로 양호한 재현성을 확인할 수 있었다. 또한 동시 분석법의 정확성을 검증하기 위하여 어린이 기호식품 및 건강기능식품의 표시함량 모니터링을 수행한 결과, 모두 표시기준에 적합함을 확인할 수 있었다.

The purpose of this multiplex PCR assay is establishment and application for rapid and simultaneous detection of six pathogens related with insect diseases. Five pathogens were chosen based on the insect disease incidence rate in South Korea and specific primers of those pathogen were designed to detect insect diseases and test multiplex PCR for detecting Fungi; Beauveria bassiana(Bb), Metarhizium anisopliae(Ma), Bacteira; Bacillus thuringiensis(Bt), Pseudomonas aeruginosa(Pa), and Serratia marcescens(Sm). This research carried out the results detecting five kinds of insect pathogen of P. b. seulensis by multiplex PCR. Multiplex PCR is effective and save time to detect simultaneously these insect pathogens and multiple infections to prevent insect disease. In our study, using multiplex PCR, we demonstrated that P. b. seulensis was frequently infected with S. marcescens and co-infected with M. anisopliae in more than 80% of cases, indicating that such an analysis can be useful for pathogen identification, especially if different pathogens produce similar symptoms.

A Duplex polymerase chain reaction (PCR) was developed for the simultaneous detection and differentiation among Nosema spp. and Vairimorpha spp. from Lepidoptera insects. Two sets of primers were selected from different genomic sequences to specifically amplify an 831 bp amplicon within the SSU rRNA gene, specific for both Nosema spp. and Vairimorpha spp. (MSSR primer); a 542 bp amplicon within the SSU rRNA gene, specific for Vairimorpha spp. (VSSU primer). Using the primers in conjunction (duplex PCR) it was possible to detect Nosema spp. and Vairimorpha spp. to differentiate between them. The sensitivity of this PCR assay was approximately 10 spores per milliliter. It is proposed that the duplex PCR is a sensitive, specific and rapid tool that can serve as a useful differential diagnostic tool for detecting Nosema spp. and Vairimorpha spp. in Lepidoptera insect.

저작권 규율체계의 목적은 단순히 저작권을 보호하자는 것이 아니라 저작물의 이용을 촉진함으로써 궁극적으로 인류 문화의 발전에 기여하고자 하는 것이라고 할 수 있으며, 저작권법을 포함하는 정보법 질서의 궁극적 가치는 자유로운 정보유통을 촉진함으로써 개인의 자유와 민주주의를 실현하는 것이라고 볼 수 있는바, 이러한 점에서 저작권의 보호는 상대적일 수밖에 없으며 상위법인 헌법은 물론 다양한 규제법과 긴장관계를 가지기도 한다. 저작권법 체계상 문화 발전이라는 공익은 다른 법률에 의해 촉진, 보완되기도 하지만 다른 법률상 공익과의 균형을 확보하기 위하여 때로는 제한되기도 한다. ICT 규제법의 경우에도 대부분의 법제는 기술발전을 위한 창의적 활동을 장려하기 위하여 저작권의 창출과 보호를 지원하는 것이지만 일정한 경우에는 저작권을 제한함으로써 다른 공익 목적을 달성하고자 하는 경우도 있다. 저작권 제한의 대표적인 경우가 방송법상 지상파방송 의무 동시재송신의 경우 동시중계방송권 배제 규정이다. 방송법상 동시재송신 조항은 방송의 공익성으로서 보편적 서비스에 대한 접근권과 이를 통한 민주적 여론 형성을 목적으로 하고 있는바, 방송법상 동시재송신에 대한 저작권의 제한은 저작물의 이용을 촉진함으로써 표현의 자유와 방송의 공익성을 도모하기 위한 것으로 헌법적 정당성이 인정된다고 할 수 있다. 결론적으로 표현의 자유의 우위와 방송의 공익성을 고려하여 방송법상 동시재송신에 있어서 저작권 문제를 정리하는 경우 저작물의 활용 확대라는 차원에서 복잡한 저작권 권리관계의 간소화와 명확화, 방송분야 저작권 이슈에 대한 전문규제기관의 역할 강화라는 방향으로의 개선이 필요할 것으로 판단된다.

Odor compounds and air-born microorganisms are simultaneously emitted from various aeration processes such as aerobic digestion, food-waste compositing, and carcass decomposition facilities that are biologically-treating wastes with high organic contents. The air streams emitted from these processes commonly contain sulfur-containing odorous compounds such as hydrogen sulfide(H2S) and bacterial bioaerosols. In this study, a wet-plasma method was applied to remove these air-born pollutants and to minimize safety issues. In addition, the effects of a gas retention time and a liquid-gas ratio were evaluated on removal efficiencies in the wet-plasma system. At the gas reaction time of 1.8 seconds and the liquid-gas ratio of 0.05 mLaq/Lg, the removal efficiency of bioaerosol was approximately 75 %, while the removal efficiency of H2S was lower than 20 %, indicating that the gaseous compound was not effectively oxidized by the plasma reaction at the low liquid addition. When the liquid-gas ratio was increased to 0.25 mLaq/Lg, the removal efficiencies of both H2S and bioaerosol increased to greater than 99 %. At the higher liquid-gas ratio, more ozone was generated by the wet-plasma reaction. The ozone generation was significantly affected by the input electrical energy, and it needed to be removed before discharged from the process.

The steam table in saturated and superheated region was modeled simultaneously using the neural networks. A variable was introduced to distinguish between the saturation and the superheat. The relative errors were compared with the quadratic spline interpolation method. The relative errors by the neural networks were superior to those by the quadratic spline interpolation method over almost all ranges of temperatures and properties. The overall errors in the saturated region were better than those in the superheated region. From the analysis, it was confirmed that the neural networks could be a very powerful tool for simultaneous modeling of superheated and saturated steam table