톱다리개미허리노린재[Riptortus pedestris (Fabricius) (Hemiptera: Alydidae)]의 방제를 위해서 집합페로몬트랩이 일부 사용되고 있으나 실제 방제 효과가 있는지에 대한 연구는 드믄 편이다. 2013년 안동지역 콩 경작지에서 집합페로몬트랩의 설치시기에 따른 노린재 방제 효과에 대한 연구 중 트랩설치가 콩의 재배 후반부에 톱다리개미허리노린재 밀도를 오히려 상승시키는 것을 발견하고, 이를 실험적으로 확인하기 위해 안동대학교 시험포에서 추가 연구를 수행하였다. 본 발표에서는 그 결과를 보고하고 토론하고자 한다.

비래해충인 멸강나방 성충의 발생을 실시간으로 예찰하기 위하여, 성페로몬 트랩에 원격감지장치를 설치한 트랩의 운용 효율성을 검정하였다. 원격감지트랩은 콘트랩 기반에 곤충의 유입을 광차단으로 감지하는 센서부, 신호전송부, 태양열기반의 전력공급부, 트랩 지지대, 웹페이지 기반의 자료수집부로 구성되었다. 연구과정에서 멸강나방 수컷 성충의 밤 시간대 포획의 일일주기성을 이용한 신호감지 시간대 축소, 신호발생 프로그램 조절, 1시간 간격의 전송간격, 신호전송 프로그램 변형 등을 통해 포획 나방수와 감지신호 발생 수의 격차를 줄여 최종적으로 신호 정확도를 92% 이상으로 개선하였다. 또한 실제 멸강나방 성충의 발생양상과 원격감지트랩에서의 신호 발생양상의 상관계수가 0.98이상으로 원격감지트랩에서의 신호 발생이 멸강나방 발생 양상을 정확하게 반영하는 것으로 나타났다. 2011~2012년 멸강나방은 연중 여러 번 발생되는 것이 관찰되었는데, 모두 짧은 기간 동안 발생되어 가파른 피크가 특징이었다.

F. occidentalis causes to decrease the yield of chrysanthemum flower economically because it is the major crop pest injuring the flower directly at flowering period in chrysanthemum artificial houses. The control of it with chemicals is very difficult because the resistance of it to chemicals is so high. F. occidentalis began to occur at early-May and increased rapidly mid-May after in standard chrysanthemum artificial houses, Yesan, Chungcheongnam-do. In case of a farm, the density of it increased continuously in spite of chemical control . The times of chemical control to depress the density of it was 6 or 7th for cultivation period of chrysanthemum. We put the flowering yellow pot-mum already (trap plant attracting F. occidentalis) in standard chrysanthemum PVC-houses and set the yellow sticky cards up at an interval of 5m from trap plant(TP). The more yellow sticky trap near to trap plant, the more F. occidentalis was attracted to yellow sticky trap. TP was more attractive to adult F. occidentalis up to distances of 10m. The numbers of it attracted to yellow sticky traps at different distance from TP was not different significantly when standard chrysanthemum bloomed. We put the TP in standard chrysanthumum PVC-house at an distance of 20m and released the natural enemies (Orius laevigatus) on TP at 3 times, an interval of 7 days. And then we set yellow sticky traps up at an distance of 5m from TP and compared the plot with TP+natural enemy(NE) to the plot without them. The density of F. occidentalis was rather lower at TP+NE plot than the plot without at the formative period of flower bud of standard chrysanthemum. Therefore, The yellow flowering chrysanthemum possesses great value of TP attracting F. occidentalis in chrysanthumum PVC-house up to flowering period of cultivating chrysanthemum, and if it is used with natural enemy and chemicals simultaneously, farmers can minimize the quantity of chemicals used.

This study was examined for activity of aggregation pheromone trap with residual effect of insecticides against Riptortus pedestris. Time of escape was from aggregation pheromone trap researched 49.74 min (ET99) and 65.01 min (ET99) with 1st and 2nd instar nymph respectively. Six insecticides for the stink bugs were treated to 3 different materials (wood, metal, and plastic). Bifenthrin and fenitrothion were showed 100% insecticidal activity at 48 h to all developmental stage of R. pedestris. Residual effect of bifenthrin and fenitrothion on plastic material were showed 100% insecticidal activity over 15 day after treatment with 1st, 3rd instar nymphs, and adults except 5th instars nymph. In field test using plastic material trap with two pesticides, 1st instar nymphs and adults were showed 100% mortality until 10 day after treatment. These results indicate that R. pedestris might be managed using aggregation pheromone trap with insecticides.

As a part of disease vector and possible overseas inflow caused by global warming monitoring around airport and port area, the distribution of domestic mosquitoes was investigated using BG-sentinel trap. Mosquitoes were collected during 5-days per each area in early September, using 10 traps in Cheongju (airport) and 15 traps in Pyeongtaek (port). The numbers of mosquitoes collected in Cheongju and Pyeongtaek area were 1,762 including 10 species and 1,042 including 11 species respectively. In Cheongju, Aedes albopictus (657 individuals, 37.3%) and Culex pipiens complex (415 individuals, 23.6%) were dominant species; while in Pyeongtaek, Cx. pipiens complex (806 individuals, 77.4%) was dominant followed by Anopheles spp. (90 individuals, 8.6%) and Ochlerotatus koreicus (79 individuals, 7.6%). Similar species were collected in both Choengju and Pyeongtaek area. Although most species collected in both Cheongju and Pyeongtaek area were the same, Cx. inatomii was only collected in Pyeongtaek. In case of the Genus Aedes, more number of Ae. albopictus was collected compared to Oc. koreicus in Cheongju; while Oc. koreicus was more in Pyeongtaek. In case of the genus Culex, the individuals belonging to the vishinui group were collected such as Cx. bitaeniorhynchus, Cx. tritaeniorhynchus and Cx. orientalis. This study may provide basic information for efficient prevention of vector mosquito and potential overseas inflow of diseases, also we would try to expand different area in Korea.

제주도에서 곶자왈이라 부르는 지대는 암괴상 아아 용암류의 특징을 잘 보여주 는 “곶자왈 용암(gotjawal lava)”이 놓여 있어 강수가 곧바로 지하수로 유입되는 투 수성과 식생다양도가 매우 높은 지역이다. 현재 한경-안덕 곶자왈 주변에는 대규모 건설이 끊이지 않고 있어 서식의 훼손이 불가피한 만큼 한경-안덕곶자왈 내에 분포 하는 지표성 딱정벌레목(Coleoptera)의 종풍부도, 종다양도를 파악하여 기초적인 자료를 제공하고자 실시하였다. 본 조사는 2013년 4월부터 9월까지 도너리 곶자왈 지대 6곳(G1, G2, G3, G4, G5, G9), 병악 곶자왈지대 3곳(G6, G7, G8)을 대상으로 월 3회 실시하였으며, 각 지역마다 함정덫(Pit-fall Trap)을 5m 간격으로 5개씩 설 치하여 닭내장으로 유인하였다. 조사결과 1목 17과 61종이 채집되었으며 바가지 촉각풍뎅이(Phaeochrous emarginatus) 2011개체, 폭탄먼지벌레(Pheropsophus jessoensis) 456개체, 왕딱정벌레(Carabus fiduciarius kirinicus) 412개체, 모가슴 소똥풍뎅이(Onthophagus fodiens) 228개체, 렌지소똥풍뎅이(O. lenzii) 135개체, 멸종위기Ⅱ급 보호종인 애기뿔소똥구리(Copris tripartitus) 97개체 등 총 4,060개 체가 조사되었다. 종풍부도(Richness Index, RI)에서 다수종(Abundant)은 바가지촉 각풍뎅이, 왕딱정벌레, 폭탄먼지벌레였고, 검정명주딱정벌레(Calosoma maximowiczi), 팔점박이먼지벌레(Lebidia octoguttata), 남방폭탄먼지벌레(Pheropsophus javanus) 등 51종은 희소종(Rare)으로 나타났다. 각 지역별 종다양도(H’)는 G5(2.11), G9(2.04), G3(1.94), G1(1.92), G8(1.56)순이며 월별 종다양도는 7월(2.42), 5월 (2.32), 9월(2.29), 6월(2.19), 4월(1.5) 순으로 나타내었다.

Riptortus pedestris (Hemiptera: Alydidae) is known to occur in barley field during spring. But, it is unlikely that R. pedestris can find host resource to reproduce in the spring season. To test this hypothesis, we assessed the reproduction of R. pedestris population collected from field from March to September. Riptortus pedestris were collected at intervals of three days from 15th of each month. Females of R. pedestris collected from trap were provided with just water in a breeding dish, and eggs laid were collected daily. Quality of R. pedestris eggs at each month was also evaluated by providing them as host resource to two egg parasitoids. The collected eggs were divided into three groups and tested for hatchability and parasitism by two egg parasitoids, respectively. As a result, R. pedestris population collected in March and April did not reproduce, and fecundity and proportion of reproduced females were the highest in August. But, neither hatchability nor parasitism rate of R. pedestris eggs show significant difference among the sampling months. In conclusion, R. pedestris starts to reproduce from May, and fecundity reaches highest on August. No change in egg quality was found among the study periods.

The species diversity of macrolepidoptera (larger species of moths) was surveyed using light traps at forests near the Forest Environmental Research Institute located in 9 provinces (Gyeonggi, Gangwon, Chungbuk, Chungnam, Jeonbuk, Jeonnam, Kyeongbuk, Kyeongnam, Jeju) in 2012. Sampling was conducted weekly or biweekly from April to October. Using light traps in 8 forests, 2,961 individuals belong to 530 species from 20 families were collected. The species richness between study sites ranged from 4 (Kyeongnam) to 258 (Chungbuk). Similarly, abundance was the highest at Gyeonggi (1,235 individuals) and lowest at Kyeongnam (11 individuals). Species diversity (H’) was the highest at Chungbuk (5.14) and lowest at Kyeongnam (1.29). Composition of macrolepidopteran species also changed seasonally, showing regional variations. Dominant species in 9 study sites were Lamoria glaucalis in Gyeonggi, Ivela auripes in Gangwon, Idiotephria amelia in Chungbuk, agrotis tokionis in Chungnam, Acosmeryx naga in Jeonbuk, Glyphodes perspectalis in Jeonnam, Spodoptera depravata in Gyeongbuk, Xestia dilatata in Gyeongnam and Lithosia quadra Jeju. Among the species, Ivela auripes, Lamoria glaucalis, and Glyphodes perspectalis were known to be sporadic forest insect pests. Therefore, continuous monitoring can be needed to prevent the potential outbreaks of such insect pests.

모기 채집기인 유문등(Black light trap)을 이용한 우사에서의 말라리아 매개모기 방제효과를 확인하기 위해 모기 주 발생 시기인 2012년 7월 26일부터 8월 2일 사이 말라리아 발생지역인 강원도 철원군에서 조사를 실시하였다. 폐쇄형 우사의 경우, 하룻밤에 유문등 한 대당 채집된 말라리아 매개모기류는 평균 27,220마리(최대 33.931마리, 최소 659마리)였으며 모기가 가장 많이 채집된 유문등의 위치는 우사 내 가장 구석진 곳이었다. 개방형의 경우에는 하룻밤에 유문등 한 대당 채집된 말라 리아 매개모기류가 평균 22,751마리(최대 31,176마리, 최소 13,727)로 폐쇄형 우 사에 비해 다소 낮았고, 모기가 가장 많이 채집된 유문등의 위치는 축사입구 구석진 장소였다. 결론적으로, 조사기간 중 유문등 한대로 하룻밤에 말라리아 매개모기를 방제할 수 있는 모기는 평균 2천마리 이상이었고 모기가 비교적 많이 채집되는 유 문등의 위치는 소의 무리와 가까우면서 안정된 장소였다. 우사에서 유문등을 이용 한 모기 방제는 유문등의 설치 장소, 설치 높이 및 설치 거리 등의 기술적인 면을 고 려하여, 설치된 유문등에 대한 지속적인 유지관리를 할 경우 매우 효율적인 친환경 적 모기방제법이라고 판단된다.

Riptortus pedestris (F.) inflict losses on a wide range of crop plants. Biological control is an alternative approach to reduce the bean bug population. Scelionid Gryon japonicum (Ashmead) and Encyrtid Ooencyrtus nezarae Ishii are the major egg parasitoids of R. pedestris. The aggregation pheromone of R. pedestris serves as a kairomone to its egg parasitoids. A new tool was developed using aggregation pheromone in a trap which attracts both con-specific bugs and the parasitoids. Non viable host eggs were added with the trap so as to provide resource for the attracted parastioids to multiply. Several studies were conducted to develop this new tool for trapping of the bugs and enhancing parasitism simultaneously. Aggregation pheromone trap added with host eggs was found to increase temporal parasitism in field by 44% compared to the parasitism without host eggs. Higher parasitism (up to 91%) was found in the sites where aggregation pheromone trap added with host eggs was installed compared to control sites. In order to improve this tool, a new device with twelve sections to hold eggs and to be attached to the pheromone trap was designed to provide maximum exposure of the eggs to the parasitoids and to minimize the competition among the parasitoids. Parasitism and number of female R. pedestris trapped in new device attached trap were higher compared to the conventional. With better performance in trapping R. pedestris and enhancing parasitism, the tool would serve as an IPM technique that targets killing the pest and enhancing activity of the pest’s natural enemy simultaneously.

톱다리개미허리노린재 (Riptortus pedestris) 상용 집합페로몬을 이용하여 포획할 때 유인되어지는 거리를 규명하고 이를 이용한 대량포획으로 콩 피해 경감 효과를 조사하였다. 집합페로몬 트랩의 재포획 비율은 최초 방사 지점에서 40 m 이내의 트랩에서는 전체 재포획 개체의 76.1%, 80 m 이내에서는 89.0%가 포획 되었으며, 트랩 설치 방위 간에 재포획 개체수는 큰 차이가 없었다. 대량 포획을 통한 피해 경감 효과를 알아보기 위해 집합페로몬 트랩을 농가 포장에 설치한 결과, 트랩수가 증가 할수록 톱다리개미허리노린재 총 포획수는 직선적으로 증가하였다. 페로몬 트랩 당 포획 할당 면적이 770 m2인 포장에서 10주당 수량이 261.8 g 으로 가장 높았으나, 트랩 당 할당 면적과 수량과의 관계에는 일정한 경향이 없었다. 트랩 당 포획 할당 면적에 따른 종자 형태 비율은 트랩 당 포획 할당 면적이 385 m2와 770 m2 인 포장에서 건전한 A형 종자의 비율이 62% 근처로 높았고 약한 피해립인 B형 종자의 비율이 27% 근처로 가장 낮았다. 톱다리개미허리노린재 집합페로몬 트랩을 이용한 피해 경감 효과는 포장 면적 385∼770 m2 당 1개를 포장 주변에 적절히 설치 운용 한다면 건전립의 비율을 높이고 약한 피해립의 비율을 어느 정도 감소시킬 수 있는 수단으로 사용할 수 있을 것으로 판단된다.

In the case of one standard-mum polyvinyl house, the density of F. occidentalis reached its peak in early June and decreased after the chemical control, which was carried out periodically. In the case of another standard-mum polyvinyl house, the density of F. occidentalis increased continuously in spite of the periodical chemical control after planting. In both polyvinyl houses, the density of F. occidentalis began to increase mid-May. The numbers of F. occidentalis collected on a tray with wet paper by heating the flowers of pink, white, and yellow Chrysanthemum standard mums were 18.4, 56.6, and 52.6 after flowering, but in the case of the leaves, the numbers were 7.8, 16.6, and 15.4. Also, the numbers of F. occidentalis collected on a tray with wet paper by heating the buds of pink, white, and yellow Chrysanthemum standard mums were 15.2, 45.8, and 41.6, but in the case of the leaves, the numbers were 2, 8.8 and 3.4. In the Y-tube olfactometer test, the frequency of F. occidentalis’ visits to one side arm of the Y-tube olfactometer was higher in the odor cue of the white flower than of the yellow, red, and violet flowers, but in the other combinations without white flowers, the frequency was higher in the odor cue of the violet and red flowers than of the yellow flowers. In the case of the four-choice olfactometer test, in the same visual cues as the odor cues of the pot mum flowers, the frequency of F. occidentalis was higher in the yellow flower than in the other flowers (white, red, and violet) in all the observation times (10, 15, and 20 minutes). In the case of the visual cues with disturbed odor cues, F. occidentalis preferred the yellow color to the other colors in all the observation times (10, 20, and 40 minutes). F. occidentalis preferred the white color to the other colors when the same odor cues as the flower color were provided, but the odor cue of the yellow flower was provided only without the color cue.

최근 들어 하우스재배 감귤을 중심으로 발생이 증가하고 있는 귤애가루깍지벌레에 대해 효율적인 발생예찰과 함께 방제시기를 결정하기 위하여 합성 성페로몬을 이용하여 시기별 발생특성을 조사하였다. 귤애가루깍지벌레의 수컷 성충은 합성 성페로몬뿐만 아니라 색에도 반응하였다. 성페로몬의 농도는 5.0 mg까지 농도가 증가할수록 유인력이 높았다. 귤애가루깍지벌레 수컷 성충은 연 4회 발생되고 있었으며, 월동 후 수컷 성충이 처음 발생되기 시작하는 시기는 4월 중하순이었다. 수컷 성충발생이 가장 많은 시기는 제 1세대 발생기인 7월 상중순이었다. 성페로몬트 랩에 유인된 귤애가루깍지벌레 수컷 성충은 한 세대 내에서 3회의 발생성기가 나타났으며, 이는 귤애가루깍지벌레가 1~3령 약충으로 월동하기 때문인 것으로 추정되었다. 트랩에 유인된 수컷 성충의 밀도와 나무의 가지당 평균밀도와는 높은 정(+)의 상관을 갖고 있었다. 수컷 성충의 최대 유인시기를 이용하여 1령 약충 발생 시기를 추정할 수 있었으며, 그 적산온도는 산란전 기간과 비슷한 350DD이었다.

This study was conducted to see the initial increasing time of population of citrus leafminer(CLM) in spring and early summer seasons in 2007-2011. CLM adults were monitored using sex pheromone trap which is mainly composed of Z,Z-7, 11-hexadecadienal in the 4, 8 and 5 orchards of the east, south and north part in jeju island, respectively during 2007-2011 seasons. The traps and lures were changed at 10 days and 1 month interval, respectively. Though CLMs were captured on traps from March or April, it was 17, 28 and 28 May in 2007, 16, 26 May and 5 June in 2008, 8, 26 and 26 May in 2009, 27 May, 7 and 7 June in 2010 and 25 May, 2, 2 June in the south, east and north part of Jeju Island, respectively that the CLM population rapidly began to increase toward peaking. The accumulated temperature except less than developmental zero, 12.1 Ujiye T 1990). from January was 248.0, 270.5 and 289.7 in 2007, 193.2, 203.8 and 261.4 in 2008, 202.0, 245.5 and 259.9 in 2009, 261.4, 192.2 and 268.3 in 2010 and 243.4, 246.3 and 252.4 in 2011 at that time. After May overwintered CLM adults are little possible to be counted to the number of captured CLM because adult longevity is about 100 in degree days (Lim 2006). CLM populations in the south part of Jeju Island in first CLM adults increasing periods during 2007-2011 is higher than other parts.

본 연구에서는 비점오염저감장치를 이용하여 밭 시험포장에서의 저감효과를 평가하기 위하여, 경남 사 천시 용현면에 시험포장을 설치하고 포장실험을 수행하였다. 시험포장은 6개의 시험구와 3개의 침사구로 구성되어 있으며, 각 시험구의 크기는 가로 5 m, 세로 22 m의 크기로 각 시험구의 말단에는 유출량을 측정하기 위한 수위계를 설치하였고, 포장에서의 강우량을 측정하기 위해 강우계를 설치하였다. 인공 강우 실험을 통해 유출수의 시간변화에 따른 수질을 측정한 결과 초기 유출시 SS, TOC, T-N, T-P, COD, NTU의 농도는 각각 15.00, 1.54, 5.27, 0.07, 4.72, 0.45 mg/L로 나타났으며, 4시간 후의 농도는 각각 1.00, 0.94, 4.06, 0.01, 0.60, 0.33 mg/L로 측정되었다. 대부분의 수질측정값이 초기 유출 시 높고 시간이 흐름에 따라 감소하는 경향을 보였다. 인공강우에 의한 유출실험 결과, 침사구에서의 부유물질은 시간이 흐름에 따라 눈으로 식별 가능할 정도로 줄었으나 각 수질측정값은 불규칙한 경향을 보였으며, 이는 샘플링 시료에 포함되는 부유물질의 양이 일정하지 않았기 때문으로 풀이된다. 향후 추가적인 연구를 통해 밭비점오염원 저감효과를 평가하고 제어대책을 개발하게 되면 밭에서의 비점오염 배출을 최소화시켜 수질개선에 기여할 것으로 사료된다.

Riptortus clavatus is one of the most serious pests affecting the cultivation of sweet persimmon, which is one of the most important export fruits in Korea. A pheromone-baited trap has been used in attempts to control this pest. Although the trap showed relatively good trap efficacy, it was often observed that R. clavatus walks around and rests on the surface of the trap rather than entering the trap. In the present study, the development of a more efficient pheromone-baited trap for R. clavatus was pursued by investigating 3 different traps: the fish trap, the pyramidal trap, and the symmetry trap. The fish trap captured the lowest number of R. clavatus, and the pyramidal trap showed moderate capture efficacy. Significantly greater numbers of R. clavatus adults were captured in the symmetry trap. Significantly increased trapping efficacy of the symmetry trap would be useful for mass trapping of R. clavatus.

Mungbean is an important crop in Nepal. Heteropteran bugs, Nezara virudula, Dolycoris baccurum, and Melanacanthus scutellaris are significantly influencing qualitative and quantitative loss in soybean and mungbean. Though pesticide application is common practice for management of these bugs, pesticide avoidance by the bugs stalls proper management. As an alternative, trap cropping with variety mostly preferred by the bugs as trap crops along with the main crop is considered. Selection of most preferred mungbean variety was performed with six (VC 6173 A; VC 6153 B-20G; VC 3960 A-88; Kalyan; Pratikchha; Saptari local) relative to Saptari local variety. Significant difference was recorded in number of bugs occurrence among the six varieties with maximum number of bugs in VC 6173 (3 bugs/plant) which showed lowest yield. For application, three different trap crop designs were employed in the field. There was significant difference between the main crop i.e. variety Pratiksha and the variety VC6173A used as trap crop in two designs. In conclusion VC6173A is a probable trap crop and the two designs can be employed in the field.

The Black pine bast scale, Matsucoccus thunbergianae is one of the most serious in black pine, Pinus thunbergii forests in Korea. Since this pest was first reported in Goheung, Korea in 1963, which is gradually spread into neighboring regions and now occurs in many regions of the southern and eastern part of the Korean peninsula. The monitoring for distribution of M. thunbergianae was able to observed by naked eye egg sacs and pupa of male on the host until now. Therefore, this monitoring was very difficult in the low density of M. thubergianae. This experiment was conducted to use simple and practical moving cross-shaped flat trap for monitoring of M. thunbergianae. The monitoring of M. thunbergianae using the device was carried out to southern regions of the Korean peninsula. The first emergence of male showed mid. March in Namhae and late march in Busan, Jinju and Pohang. The peak of emergence showed late March in Namhae and early April in the other regions. When the number of M. thunbergianae intermediate nymph showed 58~59, 11~44 and 8~25 on 39.25 ㎠ bark area of the black pine, Pinus thubergii for 1 week, the number of captured its male adult was 58~83, 67~488 and 1~55 on the moving cross-shaped flat trap (10× 13㎝), respectively. The low density of M. thunbergianae was some few the number of capture, but there were no significant difference in its high density. Also, the number of captured its male adult was no significant in the different color (yellow, red, white and blue) of the moving cross-shaped flat trap.

A remote sensing pheromone trap called IT pheromone trap (Korean patent: 10-0982563) was applied to monitor overwintering population changes of the oriental fruit moth, Grapholita molesta, for three successive years in apple orchards. Males of the overwintering populations were attracted during April and May. However, the occurrence peak was delayed and extended to early June in 2010, at which the average spring temperature was significantly lower than the previous years. These overwintering populations could be monitored by the remote-sensing pheromone trap. When the remote-sensing pheromone traps were deployed to apple orchards of different provinces in Korea in 2010, the maximal overwintering populations of G. molesta were monitored at May in all areas. However, the population sizes monitored were significantly different among different localities. This study suggests a practical application of IT pheromone trap to monitor G. molesta in field conditions.

Phenotypic traits (physiological characteristics and somatic incompatibility) and genotypic traits (target region amplification polymorphism TRAP) were used to survey the diversity in Chinese Auricularia auricula systematically, which consisted of 32 main cultivated strains. The 27 important and stable physiological indexes were evaluated; somatic incompatibility test (SIT) reaction was described from three aspects: type, pigment, and intensity while intensity clustering alone revealed the phenotypic diversity among the 32 strains;16 pairs of TRAP primer combinations produced 535 unambiguous and reproducible DNA fragments, of these 524 (97.9%) were polymorphic. The phylogenetic trees were constructed by Unweighted Pair-group Method with Arithmetic Averages (UPGMA), which distributed the test strains into four to six major groups respectively. The principal coordinate analysis (PCO) of the three methods (physiological characteristics, SIT intensity and TRAP) exhibited highly similar clustering patterns, revealing that all the test strains can be divided into three groups considered significantly correlated with geographical regions. Results revealed the occurrence of relatively low diversity of A. auricula in the study. The cultivated strains in the same region are more similar physiologically and some strains are suspected to be synonymous. This study suggests that the value for estimating diversity are represented by TRAP＞SIT reaction intensity＞physiological characteristics in A. auricula, and PCO analysis can provided more effective and visible information than the UPGMA clustering dendrogram. Our finding will facilitate future germplasm resources management and breeding program of A. auricula in China.