This study was conducted to evaluate the effects of ImmunoSEB as an immune-booster additive on the performance of broiler chicks. A total of 1150 broiler chickens were randomly allotted to three treatments on the basis of body weight (10 pens per treatment with 40 broilers in each pen): the control (CON), CON + ImmunoSEB 0.025% in feed (SEB25), and CON + ImmunoSEB 0.050% in feed (SEB50). The experiment was conducted for d 42 in three phases (phase 1, d 0–14; phase 2, d 15–28; and phase 3, d 29–42). There were significant differences in the average daily gain (ADG) and feed intake. The ADG at d 14 in the SEB50 treatment was greater than that in the CON treatment. The overall ADG in the SEB50 treatment was greater than that in the CON treatment. During d 0–14, the feed intake of chickens in the SEB50 treatment increased compared to that in the CON treatment. The crude protein and lysine digestibility improved in the SEB25 and SEB50 treatments compared to those in the CON treatment at d 28. Superoxide dismutase concentration significantly increased in the SEB50 treatment compared to that in the CON treatment. The interleukin-1β and tumor necrosis factor-α concentrations were higher in the cecum of chickens in the CON treatment than in the SEB25 and SEB50 treatments. A lower population of E. coli was detected in the ileum and cecum of broilers fed the SEB50 diet compared to those of broilers fed the CON diet. The overall result showed the beneficial effects of using ImmunoSEB at a dose of 0.050% in broiler chickens.

본 연구는 LED 조명의 파장이 육용종계의 생산성, 번식호르몬, 면역 수준 및 행동에 미치는 영향을 확인하고자 실시하였다. 25주령 아바에이커 192수를 공시하였다. 처리구는 백색(5,000 K), 녹색(520 nm), 황색(570 nm) 및 적색(620 nm) LED 전구를 사용하여 처리구당 6반복, 반복당 8수씩 임의 배치하였으며, 15주간 사양실험을 실시하였다. 산란율은 적색 처리구에서 유의적으로 증가하였고(P<0.05), 난중은 적색 처리구에서 유의적으 로 감소하였다(P<0.05). 수정율, 부화율, 병아리 체중, IgG 수준 및 혈액 생화학 조성은 처리구 간에 통계적인 차이가 없었다. 번식호르몬인 FSH와 LH 농도는 적색 LED 처리구에서 현저히 높게 나타났다(P<0.05). 또한 행동 관찰 결과, 적색 처리구에서 바닥 긁기, 바닥 쪼기 및 깃털 쪼기 행동이 다른 처리구에 비해 유의적으로 높게 나타났다(P<0.05). 본 연구 결과, 육용종계에 LED 점등을 사용할 경우 적색 처리구에서 번식호르몬의 증가와 산란율이 높아지는 것을 확인하였다. 그러나 적색 처리구에서 바닥 긁기, 바닥 쪼기 및 깃털 쪼기 행동이 높게 나타난 원인을 밝히기 위한 추가 연구가 필요한 것으로 보인다. 따라서 산란성적, 번식 호르몬 분비를 고려할 때 육용종계에 적색 LED 조명이 유리하다는 것을 확인할 수 있었다.

This study investigated the effects of π-ray treated feeds on the growth performance, carcass composition, internal organ weight, and sensory evaluation of broilers. One hundred and eighty broiler chicks were used as experimental animals. These were divided into four treatment groups according to the π-ray treated feed supplement level: 0% (control) 1%, 3%, and 5%. The π-ray treated feeds did not significantly affect on the body weight gain (2,484–2,602 g), feed intake (4,747–4,944 g), and feed efficiency (1.90–1.98) in broilers (p>0.05). Also, carcass protein (20.2–20.4%) and fat (4.6–4.9%) contents were not affected. The lengths (178.0, 182.7, 198.0, and 190.3 cm) and weights (38.4, 41.2, 44.2, and 38.9 g/kg of body weight) of the small intestine were slightly but not significantly higher in the π-ray treatments than in the control. Further, there were no overall effect of the π-ray treatment on the abdominal fat pad (2.3, 2.1, 2.1, and 1.8%), and cecum (6.7, 4.0, 5.6, and 6.0 g/kg of body weight) and gizzard (16.8, 15.2, 18.0, and 16.6 g/kg of body weight) weights of the broilers in the present study (p>0.05). The stickiness (2.25, 4.25, 4.50, and 4.38) and flavor (2.44, 4.25, 4.31, and 4.38) of cooked breast meat were higher in the π-ray treatments than in the control (p<0.01). This study also found that, in humans, eating cooked breast meat of broilers that were fed π-ray treated feeds had a positive effect on the blood circulation in the capillaries. Thus, the findings of this study suggest that dietary π-ray treatment has a positive effect on the sensory evaluation of broilers, without any negative effect on their body weight gain, feed intake, and feed efficiency, and that the consumption of these broilers is beneficial for humans on their blood circulation.

본 연구는 열 스트레스 (heat stress, HS)에 노출된 브로일러에서 혼합 생균제의 급여가 혈 액 생체지표, 면역반응, 맹장 미생물 및 생산성에 미치는 영향을 조사하였다. 총 400마리의 브로일러 수 컷 (Ross 308)을 각각 100마리씩 4그룹, C (대조군, 실온 25℃), HS (열 스트레스 33℃), HSP (HS플 러스 혼합 생균제 500, 750 mg/kg) 그룹으로 배치하였다. 브로일러의 증체량, 사료섭취, 사료요구율 및 면역기관 무게는 HSP 그룹에서 HS 그룹과 비교했을 때 증가하였다. 혈액 IgG, lymphocytes 농도는 HS 그룹에서 HSP그룹과 비교했을 때 증가하였고, heterophil과 lymphocyte(H:L)비율, 코르티코스테론 농도 및 폐사율은 낮았다. 맹장의 Lactobacillus는 HS 그룹과 비교했을 때 HSP 그룹에서 증가하였으나 Escherichia coli (E. coli), coliform bacteria, aerobic bacteria는 낮아졌다. 본 연구결과는 열 스트레스 에 노출된 브로일러에게 B. subtilis, S. galilaeus 및 Sphingobacteriaceae등 3가지 균주가 포함된 혼합 생균제를 급여해주면 면역반응 증진, 미생물 균형을 유지해줌으로써 폐사율을 낮추고 생산성을 개선할 수 있음을 시사해준다.

In the livestock feed industry, antibiotics are used to prevent disease, promote growth rate, and improve feed efficiency. However, antibiotic supplementation to animal feed results in increased bacterial resistance to antibiotics as well as antibiotic residues in animal products, which can negatively affect human health. Therefore, alternative sources of antibiotics are need- ed. Probiotics as an alternative to antibiotics in animal feed have been shown to increase feed efficiency and growth rate by improving microbial balance. Further, Bacillus sp. produces a wide spectrum of antibacte- rial peptides. The present study was conducted to investigate the effects of dietary supplementation with CS-32 on safety, growth rate, and feed efficiency. Antibacterial substance (5697.9 molecular weights) produced by CS-32 was isolated and purified from culture broth. Moreover, the results of minimal inhibi- tory concentration (MIC) test confirmed the excellent antibacterial effect of CS-32. In vivo, 0.1% and 1% CS-32 were fed to broiler chickens for 28 days. Feed efficiency was slightly higher in groups of chickens supplemented with 0.1% and 1% CS-32 than those of the control group. CS-32 had no significant effect on necropsy findings, hematology, or serum biochemistry, and there was no mortality. These results suggest that CS-32 among various biologically active substances may be safe and effective as a feed additive to improve growth rate and feed efficiency.

본 연구는 국내 일반 사양환경에서 ROSS 육용계의 성장능력을 조사․분석하고, 사료효율을 고려한 도계의 적정시기를 산출하며, 도체성적을 분석하여 ROSS종 육계의 국내 활용 가능성을 검증하고, 성장관련 유용유전자를 발굴하여 조기선발을 위한 육종 전략을 수립하는데 목적으로 실시하였다. ROSS308 육계는 부분육 생산에 탁월한 능력을 지닌 품종으로 국외에서는 일반적으로 42일령에 도계를 실시하지만 국내에서는 55일령까지는 도계시기를 연장해도 좋지만 그 이후에는 사료효율이 저하되기 때문에 55일령을 도계시기로 선정하는 것이 바람직하다. 일반성장능력은 일당증체량이 83.4±10.4g, 사료섭취량은 4.5±1.4g, 출하체중은 4,833.2±570.7g, 정강이 길이는 9.36±5.4㎜, 가슴중량은 1,088.6± 125.0g, 넓적다리 중량은 864.8±86.9g으로 조사되었다. 초기 성장에서 능력이 저하되는 개체들은 조기 도태를 유도하여 계군 전체의 성장 능력 및 도체 성적을 개선할 수 있을 것으로 사료되며 이를 위하여 4개의 후보유전자인 TGFBR1, TGFBR1, IGF2, POUF1 등을 활용하여 조기선발을 유도하는 것이 유용할 것으로 사료된다.

This experiment was conducted to investigate the effect of dietary supplementation of the Black Soldier Fly(BSF) larvae on growth performance, carcass rate, and fatty acid profile of chicken meat in broiler chicks. Four hundred eighty 1-d-old broilers(Ross 308) were distributed into 3 treatments with 4 pens at 30 birds per replicate. Treatments were corn-soybean meal based broiler diet (control) and the basal diet replaced with BSF at the level of 3 and 6% respectively. Viability was not different significantly. Body weight gain was higher in control than other treatments. Feed intake was not statistically different of treatments. Feed conversion ratio was lower in ontrol than other treatments. Relative weight of breast meat was significantly increased in all treated groups than in control. But, another relative weights of chicken meat cuts (thigh, wing, neck, back) were not significantly different among the groups. In conclusion, it could be suggested that dietary supplementation of Black Soldier Fly larvae could beneficially improved the performance on broiler

본 연구는 폭염 스트레스(extreme heat stress, EHS, 33±2℃)에 노출된 브로일러에서 대두유, 당밀, 아미노산, 비타민 C를 적절하게 함유하는 폭염사료 (extreme heat stress diet, EHSD)급여가 짧은 사슬지방산 및 성장능력에 미치는 영향을 조사하였다. 부화 당일 Ross 308 병아리 500 마리를 5처리구로 완전임의배치 하였다. 각 처리구는 4반복을 가졌으며 각 반복은 25마리씩 배치하였다. 처리구는 역전점등과 함께 T1 (일반환경+기초사료 basal diet, BD), T2 (EHS+BD), T3 (EHS+BD 내 우지를 대두유로 대체 및 당밀 2%를 함유하는 EHSD), T4 (EHS+BD 내 우지를 대두유로 대체, 당밀 2%, 메치오닌과 라이신을 각각 일반사료의 1.5배씩 함유하는 EHSD), T5 (EHS+BD 내 우지를 대두유로 대체, 당밀 2%, 메치오닌과 라이신을 각각 일반사료의 1.5배씩 추가 및 비타민 C 200 ppm을 함유하는 EHSD)로 구분하였다. 브로일러의 증체량은 T1, T2와 비교할 때 T4, T5에서 유의하게 증가하였다. 그리고 면역기관, F낭, 흉선, 비장의 무게는 서로 비슷하였다. 또한 혈청 IgG, IgA, IgM 농도는 T4, T5가 T1, T2에 비교할 때 높았으나 코르티코스테론 농도는 유의하게 낮아졌다. 특히 맹장의 Lactobacillus는 T4, T5가 T1, T2에 비해서 증가하였으나 Escherichia, Coliform, Total aerobic bacteria는 오히려 유의하게 감소하였다. 초산, 프로피온산 및 총 짧은 사슬지방산 함량은 T4, T5가 T1, T2와 비교할 때 유의하게 높았다.

본 연구는 연속조명과 함께 폭염 스트레스(extreme heat stress, EHS)에 노출된 브로일러에서 폭염사료 급여가 혈액지질, 성장능력, 면역기관, 혈청 면역물질, 맹장 미생물 변화에 미치는 영향을 조사하였다. 일반 환경온도 (25℃) 또는 폭염스트레스 (33±2℃)하에서 사육한 병아리는 일반사료(chow diet, CD)와 폭염사료(extreme heat diet, EHD)를 섭취하였다. 부화 당일 Ross 308 병아리 500 마리를 5 처리구 4 반복(반복 펜 당 25 마리)으로 완전임의배치 하였다. T1 (일반환경+CD), T2 (EHS+CD), T3 (EHS+CD 내 우지를 대두유로 대체 및 당밀 5%를 함유하는 EHD), T4 (EHS+CD 내 우지를 대두유로 대체, 당밀 5%, 메치오닌과 라이신을 각각 CD 의 1.5 배씩 함유하는 EHD), T5 (폭염+CD 내 우지를 대두유로 대체, 당밀 5%, 메치오닌과 라이신을 각각 CD 의 1.5 배씩 추가 및 비타민 C 300 ppm 을 함유하는 EHD)로 구분하였다. EHS 는 체중 및 사료섭취량을 유의하게 감소하였다. 혈액지질, 혈액 면역물질, F 낭, 흉선 그리고 비장의 무게는 브로일러를 EHS 에 노출하였을 때 유의하게 낮아졌다. 일반 환경온도 처리군과 비교할 때 EHS 처리군에서 맹장 Lactobacillus sp.는 낮았으나 Escherichia sp., Salmonella sp. 및 총호기성균은 높게 나타났으며 각 처리구 사이의 통계적인 유의차가 인정되었다.

A study was investigated the effects of the probiotic mixture (PM) contained Lactobacillus plantarum (L. plantarum) SY-99 2.4×109 CFU/kg and Bacillus subtilis (B. subtilis) SJ-61 2.2×109 CFU/kg on growth performance and fecal microflora counts of broiler chickens. Referred to the previous studies, a total of 160 one-day-old male broiler chicken (Ross 308) were randomly assigned to four experimental groups, which were a basal diet alone (control) and supplemented with PM 0.1 g/kg feed (T1), PM 0.2 g/kg feed (T2), and PM 0.4 g/kg feed (T3). After 28 days of administration, the average daily feed intake of T1 and T3 was significantly different compared to that of control (p<0.05). In addition, the feed conversion ratio of T2 and T3 was significantly improved compared to that of control (p<0.05). On the 28th day after administration, all treatment groups were significantly increased fecal lactic acid bacteria (LAB) counts and decreased fecal Enterobacteriaceae (ENT) counts compared to T1 (p<0.05). The results of this study indicated that the combination of L. plantarum and B. subtilis strains in the range of 0.2-0.4 g/kg feed could be used for the improvement of growth performance and fecal microflora in broiler chickens.

Probiotics, enzymes, organic acids, oligosaccharides, antioxidants, and other functional materials are actively being explored as alternatives to antibiotics. Probiotics include live beneficial microorganisms that colonize the intestinal tract and competitively inhibit attachment and growth of harmful microbes. Probiotics also increase feed efficiency by assisting in nutrient absorption and digestion. The current study was conducted in order to evaluate the effect of a new probiotic, CS-A, as a dietary supplement of a fermented product on growth performance, feed intake, and feed conversion efficiency in broiler chickens, and to evaluate its value as an alternative for antibiotics used as a feed additive. Antibacterial and anti-inflammatory effects of CS-A were investigated in vitro and the in vivo effects of a constant concentration of supplemented CS-A on growth rate and feed efficiency were evaluated. In addition, the safety of CS-A was assessed by examination of common symptoms and mortality. Determination of minimal inhibitory concentration revealed an excellent antibacterial effect of CS-A. Cytotoxicity was low and anti-inflammatory effects were achieved at the effective concentration of CS-A. Supplementation with 0.1% CS-A resulted in a feed efficiency score of 1.84 in broilers, compared to 2.00 in the control group. There were no adverse clinical findings, necropsy findings, hematology, and altered serum biochemistry parameters, and no mortality. Thus, it is concluded that CS-A is safe and effective as a feed additive.

Our previous study demonstrated that fermented four-herb combination (Ulgum, Yagmomil, Maesil and Bokbunja) with probiotics (Saccharomyces cerevisiae, Lactobacillus acidophilus, Bacillus subtilis) (hence, this additives was designated PFH) have immune enhancing and antibacterial effects in broiler chickens. The aims of the present study were to investigate the effects of PFH on growth performance and meat quality of broiler chickens. Twenty-day-old Ross broiler chickens were fed with or without PFH for 2 weeks. Body weight gain of the 2% PFH-fed group was significantly increased compared with those of the control group (*P<0.05). Moreover, body weight gain of the 1% PFH-fed group was also showed a increased tendency compared with the control group, although the difference was not significant (P=0.06). However, no significant difference in feed intake was observed among the groups. Feed conversion ratio of the 2% PFH-fed group was significantly decreased compared with those of the control group (*P <0.05). In addition, feed conversion ratio of the 1% PFH-fed group was also showed a slightly decreased tendency compared with the control group, although the difference was not significant (P=0.07). However, no significant differences in the proximate composition (moisture, crude fat, crude protein and crude ash) and the physico-chemical index (cooking loss, shearing force, water holding capacity and pH) were found among the groups. In addition, succulence, tending and flavor in meat of all 3 groups were also not different. Taken together, these findings suggest the possibility that PFH could be used as the alternative of antibiotic growth promoters by improving the performance of broiler chickens.

This study was carried out to determine the effect of dietary crude fucoidans extracted from brown algae and brown seaweed powder supplementation on performance and bacterial count on feed additives of early broiler chicks. A total 180 broiler chicks were assigned to 4 treatments. Each treatment had 15 chicks with 3 replications. The supplementation levels of fucoidan and brown seaweed powder in the experimental diets were 0.5% (fucoidan), 3.0% (brown seaweed). The results obtained summarized as follows: The body weight gain and weight of chicks fed fucoidan (0.5%) was heavy compared with control. Feed efficiency of chicks fed fucoidan (0.5%) slightly improved compare with control. But also not significantly different among treatments. Gizzard weight, length of small intestine and weight of small intestine were not significantly different among treatments. The villi height of chicks fed fucoidan (0.5%) was long compared with control. According to these data, this trial proves the fucoidan could be successfully used as the dietary supplement.

This study was carried out to compare the effect of dietary zinc gluconate, fructooligosaccharide and probiotic on body weight gain, feed intake and feed efficiency of broiler chicks. A feeding trial was conducted with a total of 216 broiler chicks. Each treatment had 18 chicks with 3 replications. The supplementation levels of each treatment in the experimental diets were 0.20% for zinc gluconate, fructooligosaccharide and probiotic, respectively. The body gain of chicks fed feed additives significantly increased (p<.05) compare with that of control. Especially, the treatment with probiotic produced higher body gain than other treatments. Average feed intake of chicks was not significantly different among treatments (p<.05). Feed efficiency of chick fed feed additives significantly (p<.05) improved compare with control and the highest in the probiotic treatment. Therefore, the feed efficiency of chicks fed fructooligosaccharide, zinc gluconate and probiotic treatment were increased by 3.5%, 4.0% and 4.5%, respectively compare to that of the control. In conclusion, dietary feed additives tended to slightly improve the body gain and feed efficiency. Especially the treatment with probiotic produced higher body gain and feed efficiency among other treatments.

In order to investigate the effects of magnetized water treated with HI-Scalper153R on body performance and ammonia concentration in feces of broiler chicks, a feeding trial was conducted with a total of 180 broiler chicks. Each treatment had 18 chicks with 5 replications. Chicks during experimental periods, broiler chicks were fed magnetized water and subterranean water, respectively. The results obtained summarized as follows: Body weight gain slightly increased for broiler chicks fed magnetized water compared with control. But, there is no significantly (p=0.36) different among treatments. Feed intake significantly (p=0.046) decreased for broiler chicks fed magnetized water compared with control. Feed efficiency was significantly (p=0.020) improved for broiler chicks fed magnetized water of each periods when compare to the control. Especially, in starter periods, the feed efficiency of magnetized water treatment increased by 10% more than control (1.80 vs 1.69). In feces, average ammonia concentration (ppm) significantly (p=0.004) decreased in the magnetized water treatment compare to control. Especially, the magnetized water treatment decreased by 2.2 times compared to the control in ammonia concentration of feces (83 vs 37ppm).