Habitat environment and food sources of fish, benthic macroinvertebrates, and brown trout (Salmo trutta) downstream of Soyang River Dam were analyzed. Water temperature at the site where brown trout was identified ranged from approximately 12.4 to 13.4°C, confirming that this environment could provide an optimal water temperature for the growth of brown trout. Most of the riverbed structures at this site had a high proportion of cobble and pebble substrates. Brown trout constituted less than 5% of the total fish population, more abundant in the upstream. The total lengthweight relationship of brown trout showed a parameter b value of 3.234, with the condition factor (K) increasing with length. Dominant benthic macroinvertebrates were Limnodrilus gotoi and Chironomidae spp. (non-red type). Stomach content analysis indicated that brown trout primarily consumed aquatic insects (R.A., 73.8%), non-insects (R.A., 23.3%), ground insects (R.A., 2.7%), and fish (R.A., 0.2%, TL: 246 mm). Correlation analysis revealed a positive relationship between total length and species preferring flowing water (p<0.05) and a negative relationship of total length with species favoring low-flow, sandy habitats (p<0.05). Larger brown trout showed active feeding behavior and resilience to flow speed and riverbed structure. The primary food source for the largest brown trout (TL: 246 mm) was Hypomesus nipponensis. Future analyses should include brown trout with a total length of 30 cm or more. Given that samples were limited, comprehensive population management will require ongoing research.

A new high-tech product - lab grown meat (LGM), has been gaining media attention while initiating public discourse on social media (SM) platforms. This netnographic study is based on a dataset of selected SM public posts, comments and discussions collected during 30 days in early 2023. The findings indicate that LGM is highly contradictory, while not being fully understood how it is produced or when it will become commercially available. The findings indicate that this novel food requires carefully designed marketing strategies: when naming a new product category; must allow transparency and sensibly explain all product’s attributes; and invest time and efforts to educate consumers, leading to higher adoption rates when launched on mass consumer markets, as an alternative to conventionally grown foods.

Research on food sources through DNA metabarcoding is being used for various organisms based on high resolution and reproducibility. In the study, we investigated the difference in food sources between pre and post-starving in the three bivalve species (Anemina acaeformis, Anodonta woodiana, and Unio douglasiae) through DNA metabarcoding using 18S rRNA V9 primer. The food source of pre-starving appeared in 87 genera, 71 families, 51 orders, 35 classes, and 22 phyla. The primary food sources were the zoo and phytoplankton, including Chlamydomonadales, Euglenales, Ploima, Sphaeropleales, and Stephanodiscales. However, all zoo and phytoplankton were not observed after starving except Schizopyrenida and Rotifera. In Levin’s niche breadth analysis, the Bi index of A. woodiana is 0.3, which was higher than A. acaeformis (0.14) and U. douglasiae (0.21), indicating that they feed on various food sources. The niche overlap of A. acaeformis was measured as 0.78 in A. woodiana, 0.7 in U. douglasiae showing a relative high value compared to other bivalves. The trophic level of A. acaeformis, A. woodiana, and U. douglasiae based on the food source information were investigated as 2.0, 2.0, and 2.5, respectively. The results of the previous study on the trophic level using stable isotopes showed 1.8 to 2.4 values were similar to the results of this study. These results suggest that DNA metabarcoding can be an effective analyzing tool for the gut content in the bivalves.

The health benefits associated with consumption of fresh produce have been clearly demonstrated and encouraged by international nutrition and health authorities. However, since fresh produce is usually minimally processed, increased consumption of fresh fruits and vegetables has also led to a simultaneous escalation of foodborne illness cases. According to the report by the World Health Organization (WHO), 1 in 10 people suffer from foodborne diseases and 420,000 die every year globally. In comparison to other processed foods, fresh produce can be easily contaminated by various routes at different points in the supply chain from farm to fork. This review is focused on the identification and characterization of possible sources of foodborne illnesses from chemical, biological, and physical hazards and the applicable methodologies to detect potential contaminants. Agro-chemicals (pesticides, fungicides and herbicides), natural toxins (mycotoxins and plant toxins), and heavy metals (mercury and cadmium) are the main sources of chemical hazards, which can be detected by several methods including chromatography and nano-techniques based on nanostructured materials such as noble metal nanoparticles (NMPs), quantum dots (QDs) and magnetic nanoparticles or nanotube. However, the diversity of chemical structures complicates the establishment of one standard method to differentiate the variety of chemical compounds. In addition, fresh fruits and vegetables contain high nutrient contents and moisture, which promote the growth of unwanted microorganisms including bacterial pathogens (Salmonella, E. coli O157: H7, Shigella, Listeria monocytogenes, and Bacillus cereus) and non-bacterial pathogens (norovirus and parasites). In order to detect specific pathogens in fresh produce, methods based on molecular biology such as PCR and immunology are commonly used. Finally, physical hazards including contamination by glass, metal, and gravel in food can cause serious injuries to customers. In order to decrease physical hazards, vision systems such as X-ray inspection have been adopted to detect physical contaminants in food, while exceptional handling skills by food production employees are required to prevent additional contamination.

Riptortus pedestris (F.) (Hemiptera: Alydidae) attacks several leguminous crops and its reproductive success is found to be affected by the leguminous host types. Ooencyrtus nezarae Ishii (Hymenoptera: Encyrtidae) is an egg parasitoid of R. pedestris and its quality attributes depend on the host egg quality. We, thus, investigated the performance of O. nezarae on eggs of R. pedestris fed on different leguminous seeds. Eggs collected from R. pedestris female adults obtained from rearing on one of the four hosts such as soybean, adzuki bean, mung bean, and cowpea seeds were exposed as a batch (n=15) to a single 4 to 5 day-old mated female O. nezarae. The eggs exposed were collected after 24 h. Number of parasitized eggs, sex ratio, development times, and adult emergence were recorded. The maximum number of eggs parasitized by O. nezarae were the eggs of mung bean fed R. pedestris. Eggs produced from the adzuki bean fed bugs had the lowest parasitization. There was no difference recorded in the developmental time of parasitoids emerged from the eggs of R. pedestris fed on different legumes. Proportion of male O. nezarae was highest on the eggs of cowpea seed fed adult bugs (26%); the lowest of that was found on the eggs of mung bean fed bugs (22.4%). Ooencyrtus nezarae can successfully parasitize eggs of R. pedestris regardless of hosts food sources. However, eggs of mung bean fed R. pedestris, among the tested legumes, were found to be of the best quality for reproductive success of O. nezarae.

곡식좀나방과는 곡식좀나방상과(Tineoidea)에 속하며 전 세계적 으로 약 3,600여종이 광범위 하게 분포하고 있는 것으로 알려져 있다. 또한 곡식좀나방과의 유충은 매우 광범위한 먹이원을 섭식하는 것으로 알려져 있는데, 이는 일반적으로 나비목의 유충이 섭식하는 식물성 먹이원을 섭식하는 종류부터 부식된 낙엽이나 썩은 나무, 버섯과 같은 균류를 섭취하는 종류, 동물성의 키틴이나 케라틴 조직을 섭취하는 종류까지 생태계에 있는 광범위한 종류의 유기물질을 섭식한다. 국내의 곡식좀나방과는 30여종이 알려져 있으며 형태학적인 분류체계를 따르는 속단위의 분류군에서 비슷한 먹이원을 공유 하는 경우가 대다수 이기 때문에 파악한 먹이원의 종류를 연구에 적용한다면 다수의 국내 미기록 종 및 신종의 추가가 기대된다. 조사는 해외의 문헌을 기초로 하였으며 문헌조사결과에 따라 먹이원의 수집 및 먹이원을 활용한 트랩을 이용하였고, 곡식좀나방과의 옷좀나방아과 2종과 버섯좀나방아과 1종의 유충 및 성충의 사진을 첨부하였다.

썩덩나무노린재는 두과작물에서 과실에 이르기까지 다양한 작물을 가해하는 대표적인 다식성 노린재의 일종이다. 과실이 썩덩나무노린재의 발육에 필요한 영양원인지 불필요한 영양원인지 아직도 분명하지 않다. 그리하여 사과와 오렌지의 단독먹이 및 콩-땅콩과의 먹이조합에 따른 썩덩 나무노린재의 발육, 사망률 및 증식률을 평가하였다. 썩덩나무노린재 약충은 사과단독먹이에서만 높은 사망률로 성충으로 발육하지 못하였다. 물 만 제공한 것은 3령으로 발육하지 못하였고, 사과단독먹이에서 4령으로 발육하지 못하였다. 하지만 오렌지단독먹이에서 14%가 성충으로 우화하 였으나, 약충의 발육기간이 약 74일로 가장 길었다. 콩-땅콩과 조합한 먹이에서 노린재의 사망률은 38~44%를 나타내었다. 총 산란수는 169~190 개 였으며, 부화률은 81~83%이었다. 따라서 사과는 썩덩나무노린재의 발육에 불완전한 먹이 이었으나, 오렌지는 발육이 지연되기는 하였으나 이루지는 것으로 평가되었다.

The brown marmorated stink bug Halyomorpha halys Stål (Hemiptera: Pentatomidae), is native to Korea, Japan, and China. H. halys is one of the major polyphagous pentatomids with a wide host range encompassing ornamental shrubs, trees, and cultivated crops such as millet, sesame, soybean, apple, yuzu, pear, cherry, and peach and inflicting losses of several crops in Korea. However, study on dietary importance of these hosts on the development rate of H. halys is sparse. We evaluated fruits of apple and orange with or without soybean plus peanuts as food sources to investigate development rate, mortality and fecundity of the stink bug. Not apple but orange only diet could support development up to adult stage but with higher mortality. First instars were found to molt into second instars without feeding. The longest time H. halys took to develop was in its fifth instar. Overall shortest developmental period (39.82) days was recorded on those fed on orange+soybean and peanut whereas those fed on orange only diet had longest development period (78.92 days). Generational mortality of those fed on diets consisting of soybean and peanuts ranged from 43 to 53%. Those only fed on water could not develop into third instars. H. halys could not develop into fourth instar in apple only diet. However, on orange only diet 20% H. halys could emerge as adults. Fecundity was measured for the first day of oviposition per female. Fecundity was recorded highest (28 eggs) on orange+soybean and peanut diets. The results suggest that H. halys require leguminous seeds in order to develop fully and lay fertile eggs.

The bean bug, Riptortus pedestris (F.) (Hemiptera: Alydidae) is one of the most important polyphagous pests in soybean in Korea and Japan. With the review of previous studies on the suitability of different diets, we additionally tested two leguminous plants as food sources of R. pedestris by assessing development and reproduction of the bugs. The tested diets include seeds of acacia, two mung bean varieties (var. Eoul and Dahyeon), and soybean (var. Daewon). Riptortus pedestris fed on mung bean var. Eoul showed higher nymphal mortality than those fed on mung bean var. Dahyeon, acacia, and soybean. On acacia, development time and preoviposition period of R. pedestris was shortest among the tested diets Also, five day fecundity the highest in bugs fed on acacia seeds. These results showed that acacia seed can be a good quality diet for R. pedestris, and both acacia and mung bean could be a suitable host resources for R. pedestris during the period when soybean, a major host crop, is unavailable.

In order to understand food sources-metabolism for the pacific oyster (Crassostrea gigas), the stable isotope ratios of carbon (δ13C) and nitrogen (δ15N) of its gut, gill, and muscle as well as potential food sources (particulate o

본 연구는 2007년 3월부터 10월까지 충남대학교 부속사육장 및 전북 남원시 운봉읍 소재 가축유전자원시험장에서 수행하였다. 공시시료는 화본과목초(gasses)는 Lolium perenne, Festuca arundinacea, Poa pratensis, Agrostis alba 및 mixed grasses의 5종과, 두과목초(legumes)는 Trifolium pratense, Medicago sative, Trifolium repens, Melilo

본 연구는 2007년 3월부터 12월까지 충남대학교 부속사육장 및 전북 남원시 운봉읍 소재 가축유전자원시험장에서 수행하였다. 공시시료는 화본과목초(grasses)는 Lolium perenne, Festuca arundinacea, Poa pratensis, Agrostis alba 및 mixed grass의 5종과, 두과목초(legumes)는 Trifolium pratense, Medicago sativa, Trifolium repens, Melilot

본 연구는 몇 가지 조사료원을 유 산양에 급여하였을 때 조사료의 중류에 따른 채식습성을 구명하고자 2007년 3월부터 10월까지 충남대학교 부속사육장에서 수행하였다. 공시시료는 화본과목초 (grasses)는 Lolium perenne, Festuca arundinacea, Poa pratensis, Agrostis alba 및 mixed grass (혼합목초)의 5종과, 두과목초(legumes)는 Trifolium pratense, Medicago sa

Recently, the importance of food safety is increasing due to numerous junk food. Junk food means to violate the law in stage such as production, manufacture, distribution, and sale of food. Many crop plants are processing as foods including bread, noodle, and other foods for supporting nutrition to human. For example, rice is one of the most well-known food crops in the world, and processed rice is being mixed with other processed crops to health food. The object of this study is to detect amount of specific grains, i.e. rice from processed foods mixed with other cereals. This experiment was performed to the following two steps: 1) designed the specific primer sets based on chloroplast DNAs, 2) amplified products using real-time PCR. We designed eleven primer sets within chloroplast DNA of rice, and then the confirmation of primer efficiency was to amplified with rice genomic DNA using real-time PCR. In addition, these primer sets were applied in other crops such as wheat, maize, and adlay to confirm specificity to rice. The rice specific primer sets were determined by the number of amplification and the melting peak through real-time PCR. As a result, five primer sets were confirmed to uniqueness in the rice genome. In conclusion, the specific primer sets would be useful for identifying rice grain from the processed foods to eliminate junk foods and for contribution of food safety.

The spatial variability in the food chain structure of an estuary environment (Nanakita estuary, Japan) was investigated using fatty acid. Potential organic matter sources (terrestiral plants, macroalgae, benthic microalgae, dinflagellates and bacteria), sedimentary organic matters and benthic invertebrates (Nuttallia olivacea and Nereidae) were sampled in four locations with different tidal flat type. The main objective of the present study was to determine the origin of sediment and the food sources of N. olivacea and Nereidae along with small-scale spatial variability. The origin of sedimentary organic matters were mainly the fatty acid of bacteria and benthic microalgae. Especially, The organic matter of terrestrial plant origin was found the highest in station C. The diets of N. olivacea and Nereidae were found to be dominated by diatoms and terrestrial plants. Whereas, macroalgae and dinoflagellates showed little influence to benthic invertebrates. Moreover, according to principal component analysis, it is showed that benthic invertebrates in the same region are using the same food without relation with species. On the other hand, the N. olivacea and Nereidae of station D clearly contrasts with station B in terms of main food sources. From these results, it is suggested that food competition of benthic invertebrates revealed high a connection between small-scale spatial variability and food source in estuary.