Microbial proteases are more economical than plant- and animal-derived proteases due to their ease of production and high activity. This study aimed to optimize the production of proteases from fermentative food-derived microorganisms. Five strains with proteolytic activity among 50 Bacillus sp. were first screened. Two strains with high protease activity were identified: Bacillus amyloliquefaciens SRCM 102139 and Bacillus subtilis SRCM 104999. SRCM 102139 strain and SRCM 104999 strain had the highest protease activity in 0.8% glucose and 0.3% yeast extract, and in 0.8% starch and 0.1% soy peptone, respectively. The production of protease for two strains was optimized by the Central Composite Design (CCD) under response surface methodology. The optimal conditions for protease production in SRCM 102139 were 0.5% and 0.347%, pH 6.0, for carbon (glucose) and nitrogen (yeast extract springer 0202) sources, respectively, with a predicted value of 0.929 U/mL. Additionally, the optimal conditions for protease production in SRCM 104999 were 0.5% and 0.5%, pH 6.7, for carbon (starch) and nitrogen (soy peptone HSP-349) sources, respectively, with a predicted value of 0.431 U/mL. The actual protease activities of SRCM 102139 and SRCM 104999 under the established conditions were 0.926 U/mL and 0.428 U/mL, respectively, closely matching the predicted values.

Thin-film shape technology is recognized for its core technology to enhance the technology of LCD, PDP, semiconductor manufacturing processes, hard disks and optical disks, and is widely used to form coated thin films of products. In addition, resistance (electron beam filament) technology for heating is used to manufacture filament for ion implants used in semiconductor manufacturing processes. By establishing an electronic beam filament production system and developing seven specifications of electronic beam filament, it is contributing to improving trade dynamics and increasing exports to Japan through localized media of theoretical imports to domestic companies. In this study, CAE analysis was performed after setting electron beam filament specification and development objectives, facilities and fabrication for electron beam filament production, electron beam filament JIG & fixture design and fabrication followed by electron beam filament prototype. Then, the automation and complete inspection equipment of the previously developed electronic beam filament manufacturing facilities was developed and researched to mass-produce them, to analyze and modify prototypes, design and manufacture automation facilities, and finally, to design and manufacture the complete inspection equipment. In this paper, design and manufacture of electronic beam filament total inspection equipment for mass production were dealted with.

Metal material production process machinery is becoming larger due to the development of industry. Since there are many overseas manufacturers of large machines used in industrial fields, there are limitations in investigating the manufacturer's noise prediction method and measurement method. A noise map was prepared to obtain information necessary for noise reduction and to effectively manage noise companies. It was modeled with the drawings provided to prepare the noise map and the results of the actual measurement of the site. In order to improve the noise environment of workers, there was a noise reduction effect when a soundproof wall was installed between the noise source and the worker's workplace.

Codonopsis lanceolata (S. et Z.) Trautv. is mainly cultivated in Korea and China as a medicinal crop. C. lanceolata is difficult to produce plug seedlings in the summer, because C. lanceolata has a low germination rate and is vulnerable to high temperatures. Cold treatment is effective in breaking dormancy of seeds and increasing the germination rate. Shading cultivation can control the solar irradiance received by plants and reduce the damage by high temperatures and strong light. This study was conducted to examine the appropriate cold treatment period for the improving germination of C. lanceolata, and shading level during the summer seedling period. Cold treatment experiments were conducted for 0 (control), 1, 2, 3, and 4 weeks at 4°C before sowing. In the shading experiment, C. lanceolata was grown for 45 days with 0 (non-treatment), 45, 75% shading levels. Cold treatment for one week significantly improved the germination energy. The plant height, leaf area, and fresh and dry weights of C. lanceolata seedlings were significantly increased under the 45% shading level. Total root length, root surface area, and the number of root tips were significantly higher in shading treatment (45 and 75%) than in non-treatment. The C. lanceolata seedling’s compactness and Dickson’s quality index were the highest at 45% shading level. Therefore, these results recommended sowing C. lanceolata after cold treatment for one week at 4°C, and 45% shading level could stably culture C. lanceolata plug seedlings during the high temperature period.

In this study, graphene oxide (GO) was synthesized by the improved Hummers’ method. The degree of oxidation from graphite (Gi) to GO was determined through interlayer spacing calculated from X–ray diffraction. Besides, the effect of KMnO4: Gi ratios (X1), H2SO4 volume (X2), oxidation temperature (X3), oxidation time of stage 1 (X4), and oxidation time of stage 2 (X5) was screened by the Plackett–Burman model. The simultaneous impact of three factors that influenced the degree of oxidation (X1, X2, and X3) was studied by the Box–Behnken experimental model of response surface methodology to achieve suitable conditions for the GO synthesis process. The characterization of GO product was investigated via the modern analytical methods: X-ray diffraction, Raman spectroscopy, Fourier transform infrared spectroscopy, UV–Vis spectroscopy, field emission scanning electron microscopy, transmission electron microscopy, and atomic force microscopy. Inaddition, the study was also carried out on a pilot scale for orientation in industrial application with the yield of 14 g/batch.

For practical applications of graphene sheets in a variety of fields, mass production of high-quality graphene sheets is necessary. Herein, we reported a cost-effective, green, and simple approach to synthesizing mass production exfoliated graphene (EG) flakes employing electrochemical exfoliation of pencil graphite in neutral aqueous electrolytes. Pencil graphite cores of different grades were applied as anode and cathode electrodes and exposed to the electrolyte solution at a different voltage. Several parameters were examined and optimized, including pencil grade (2,4,6,8 B), applied voltage (10, 15, 20, 30 V), different inorganic electrolytes ((NH4)2SO4, Na2SO4, NaNO3, NaCl, and CH3COONa), and the concentration of electrolytes. The optimal condition was chosen by considering the mass of produced graphene and the conductivity of the graphene solution. The optimal conditions were as follow: pencil grade: 6B; applied voltage: 10 V; electrolyte type: Na2SO4; electrolyte concentration: 0.1 M. Under these conditions, the production yield was > 95% within 3 h and 9 min. The EG was characterized by utilizing FT-IR, XRD, Raman spectroscopy, FE-SEM, Cyclic Voltammetry, and Electrochemical Impedance Spectroscopy (EIS). Characterization indicates that the synthesized EG had an XRD peak at 2θ = 26.6° and an ID/ IG ratio of 0.36. Furthermore, the EG showed good conductivity when tested by cyclic voltammetry and EIS whereas the R2 values were 985.8 and 76.3 Ω for bare GCE and EG/GCE, respectively. In addition, EG effectively removed cadmium (Cd(II)) with an adsorption level of 8.72 mg/g. The results from this study suggest that EG can be scaled up and commercialized in an environmentally friendly and low-cost manner, especially in low-income countries, and using it to rectify metal ions.

시리즈 애니메이션 IP들이 사업 다각화를 위해 웹툰으로 제작되어 서비스되는 경우들이 있는데, 영세한 애니 메이션 제작사들은 웹툰으로 제작할 때 발생하는 노동집약적인 작업 과정과 비용 때문에 접근이 어려운 실정 이다. 이를 위해 함께 협업한 연구실에서 인공지능을 활용하여 <Pitchfork Park> 애니메이션 시리즈의 웹툰 변 환 작업을 자동화하는 시스템을 개발하였다. 해당 시스템을 활용하면 단순 노동적으로 작업자가 일일이 수행 하던 1) 애니메이션 컷 선별, 2) 선별된 컷 재배치, 3) 말풍선 배치, 4) 대사 배치, 5) 효과선 추가 등 상당 부 분을 자동화할 수 있다. 시스템에서 출력된 작업물을 작업자가 확인하고, 후보정 작업을 거쳐 최종 결과물을 완성한다. 기존 작업 과정에서 필요한 노동력과 시간을 절약할 수 있고, 작품의 완성도도 향상시킬 수 있다. 본 연구는 기존 웹툰 작업 과정을 분석하여 인공지능을 활용한 작업 방식의 효율성을 검증하였으며, 결과적으 로 인공지능 기술을 활용한 애니메이션의 웹툰 변환 제작 파이프라인을 구축할 수 있는 토대를 확인하였다.

Climate change is a major global problem. Oysters, one of the most representative farmed fish in Korea, are attracting attention as candidates for blue carbon, an alternative to carbon neutrality. This study is analyzed by the SSP scenarios to determine the impact of oyster aquaculture production according to climate change. Based on the analysis, future productions of oysters are predicted by the SSP scenario. Significant differences by the SSP scenario are confirmed through predictive power tests among scenarios. Regression analysis was conducted from January 2001 to December 2014. As a result of the analysis, water temperature, water temperature quadratic term, salinity, salinity quadratic term, and month × water temperature cross term were estimated as significant variables. Oyster production which is predicted by the SSP scenario based on the significant variables from 2015 to 2022 was compared with actual production. The model with the highest predictive power was selected by RMSE and MAPE criteria. The predictive power was compared with the MDM test to determine which model was superior. As a result, based on RMSE and MAPE, the SSP1-2.6 scenario was selected as the best model and the SSP1-2.6, SSP2-4.5, and SSP3-7.0 scenarios all showed the same predictive power based on the MDM test. In conculusion, this study predicted oyster aquaculture production by 2030, not the distant future, due to the short duration of the analytical model. This study was found that oyster aquaculture production increased in all scenarios and there was no significant difference in predictive power by the SSP scenario.

This study aimed to confirm the importance ratio of climate and management variables on production of orchardgrass in Korea (1982―2014). For the climate, the mean temperature in January (MTJ, ℃), lowest temperature in January (LTJ, ℃), growing days 0 to 5 (GD 1, day), growing days 5 to 25 (GD 2, day), Summer depression days (SSD, day), rainfall days (RD, day), accumulated rainfall (AR, mm), and sunshine duration (SD, hr) were considered. For the management, the establishment period (EP, 0―6 years) and number of cutting (NC, 2nd―5th) were measured. The importance ratio on production of orchardgrass was estimated using the neural network model with the perceptron method. It was performed by SPSS 26.0 (IBM Corp., Chicago). As a result, EP was the most important variable (100%), followed by RD (82.0%), AR (79.1%), NC (69.2%), LTJ (66.2%), GD 2 (63.3%), GD 1 (61.6%), SD (58.1%), SSD (50.8%) and MTJ (41.8%). It implies that EP, RD, AR, and NC were more important than others. Since the annual rainfall in Korea is exceed the required amount for the growth and development of orchardgrass, the damage caused by heavy rainfall exceeding the appropriate level could be reduced through drainage management. It means that, when cultivating orchardgrass, factors that can be controlled were relatively important. Although it is difficult to interpret the specific effect of climates on production due to neural networking modeling, in the future, this study is expected to be useful in production prediction and damage estimation by climate change by selecting major factors.

Thin-film shape technology is recognized for its core technology to enhance the technology of LCD, PDP, semiconductor manufacturing processes, hard disks and optical disks, and is widely used to form coated thin films of products. In addition, resistance (electron beam filament) technology for heating is used to manufacture filament for ion implants used in semiconductor manufacturing processes. By establishing an electronic beam filament production system and developing seven specifications of electronic beam filament, it is contributing to improving trade dynamics and increasing exports to Japan through localized media of theoretical imports to domestic companies. In this study, CAE analysis was performed after setting electron beam filament specification and development objectives, facilities and fabrication for electron beam filament production, electron beam filament JIG & fixture design and fabrication followed by electron beam filament prototype. Then, the automation and complete inspection equipment of the previously developed electronic beam filament manufacturing facilities was developed and researched to mass-produce them, to analyze and modify prototypes, design and manufacture automation facilities, and finally, to design and manufacture the complete inspection equipment. In this paper, analysis and modification of prototypes of electron beam filaments for mass production were dealted with.

본 연구의 목적은 스마트시티 구축의 핵심인 수소연료전지의 활용형태를 분석하고 해결방안을 제시하는 것이다. 수소연료전지를 활용하는 발전소의 경우 간헐성 문제가 없다는 장점 때문에 향후 가장 유망한 사용 형태로 분석됐다. 다만 많은 장점에도 불구하고 폭발 우려와 특정 수소 생산방식의 경우 이산화탄소 발생 문제 등으로 지역주민들의 반발이 지속적 으로 나타나고 있어 이를 해결하는 것이 스마트시티 구축의 주요 관건이 될 것으로 분석된다. 마지막으로 현재의 수소 생산 방식을 분석하고 이에 따른 문제점을 파악하여 스마트시티의 완전한 구축을 위한 해결책을 제시하였다.

Thin-film shape technology is recognized for its core technology to enhance the technology of LCD, PDP, semiconductor manufacturing processes, hard disks and optical disks, and is widely used to form coated thin films of products. In addition, resistance (electron beam filament) technology for heating is used to manufacture filament for ion implants used in semiconductor manufacturing processes. By establishing an electronic beam filament production system and developing seven specifications of electronic beam filament, it is contributing to improving trade dynamics and increasing exports to Japan through localized media of theoretical imports to domestic companies. In this study, CAE analysis was performed after setting electron beam filament specification and development objectives, facilities and fabrication for electron beam filament production, electron beam filament JIG & fixture design and fabrication followed by electron beam filament prototype. Then, the automation and complete inspection equipment of the previously developed electronic beam filament manufacturing facilities was developed and researched to mass-produce them, to analyze and modify prototypes, design and manufacture automation facilities, and finally, to design and manufacture the complete inspection equipment. In this paper, design and manufacture of electronic beam filament automation facilities for mass production were dealted with.

Sensory evaluation of shucking pressure, pressure holding time, seeding method, difference in full shucking rate in the aquaculture area and shucking oyster was performed using an ultra-high pressure oyster shucking machine. The reaching time for each target pressure is 2.2-2.4 MPa/sec in the range of 180 MPa to 240 MPa. had a rate of pressure rise. There was a difference of 0.5-1.7℃ in the range of 24-27℃ in the seawater temperature before and after the pressure treatment inside the pressure vessel, but there was no specific increase or decrease in seawater temperature. When only the shucking pressure is increased without the pressure holding time, the critical shucking pressure at which the oyster shell is opened and the flesh is peeled in the range of 200 to 220 MPa. When the critical shucking pressure is reached, the oyster sample in the closed vessel is expected to be shucked by about 40%. If there is no pressure holding time when judged only by full shucking, an increase in pressure of about 1.5 MPa is required to further shuck 3% of the oyster population. The oyster samples cultivated in the south coast of Korea were subject to full shucking under the conditions of 220 MPa shucking pressure and two minutes (120 seconds) of pressure holding time, and the difference in the pressure of the oysters according to the oyster seeding method and the farming area was minute. Finally, the condition of 220 MPa of shucking pressure and three minutes of pressure holding time was the best at 1.52 when the result of the sensory evaluation performed manually was set to 1.0. Next was 1.4 under the conditions of 220 MPa of shucking pressure and one minute of pressure holding time (60 seconds), and 1.3 under the condition of 220 MPa and two minutes of pressure holding time (120 seconds). Therefore, it is considered that the most desirable shucking conditions, considering the efficiency and sensory evaluation results, are the conditions of 220 MPa shucking pressure and two to three minutes of pressure holding time.

In order to apply to high-nickel cathodes for high-capacity and stability enhancement of lithium-ion batteries, the characteristics of the coating film were reviewed using the conventional nickel plating method. The surface morphology of the plating layer and the measurement of the surface roughness were analyzed according to scan size and rate using the contact mode of Atomic Force Microscopy. The hydrogen ion concentration (pH) of the electrolyte played an important role in shaping the metal ion plating. As the overpotential of the surface increased during plating, the crystals grew in a direction other than the main crystal growth direction. The increase in on-time during pulse plating appears to result in coarse particles as much of the applied current is consumed by the reduction of hydrogen ions, resulting in lower current efficiency. From the AFM image, it was confirmed that the blackening of the plated film was due to a partial overvoltage phenomenon during electrolytic degreasing. In order to be used as a high-nickel cathode, it seems that the current must be uniformly distributed on the surface of the substrate during plating.

글로벌 OTT 서비스의 등장과 급속한 확산은 기존 영상 콘텐츠 산업의 지형을 바꾸는 역할을 하고 있다. 이러한 OTT 서비스는 지역별 장벽이 존재했던 영상 콘텐츠 시장에서 콘텐츠 유통의 패러다임이 바뀌고 있다는 것과 콘텐츠 제작 영역에도 많은 영향을 주고 있다는 것을 보여준다. 결과적으로 영상 콘텐츠 산업은 제작, 유통, 소비에 이르는 산업 구조에 급격한 변동을 겪고 있다. 글로벌 OTT 사업자가 콘텐츠 유통과 소비의 중요한 부분이 되면서 나타난 중요한 제작 영역의 변화 중 하나는 전문적인 제작 능력을 갖춘 제작사들이 두각을 나타내고 있다는 사실이다. 이 논문에서는 글로벌 OTT 서비스의 급격한 확산으로 변화하게 된 제작과 유통 시스템에 대해 분석하면서 앞으로의 산업 동향에 대해서 정리하고 있다.