본 연구는 지역별, 학교별, 계절별 고등학교 급식 식단의 미생물학적 안전성 관련 주요 영향 인자를 도출하고자 식단 데이터를 수집하고 요인을 분석하여 향후 안전관리 방향을 설정하기 위해 실시하였다. 급식식단 중 PHF(Potentially Hazardous Food) 해당여부, 사용빈도가 높은 엽경채류, 식단 조리방법에 관하여 빈도 분석과 다중대응분석(Multiple correspondence analysis, MCA)을 실시하였다. 중부지역과 남부지역의 각각 3개 고 교, 총 6개 고등학교의 3, 6, 9, 12월 제공된 총 1,945개 식단의 빈도분석 결과, PHF에 해당하는 식단은 데친 나 물류, 샐러드, 해조류, 닭튀김 등이 사용빈도가 높았다. 조리방법은 가열 여부에 따라 단순 가열, 단순 비가열, 혼합 여부에 따라 가열한 식재료와 가열한 식재료의 혼합, 비가열한 식재료와 비가열한 식재료의 혼합, 가열한 식재료와 비가열한 식재료의 혼합으로 구분하였는데, PHF 제품 내 단순 비가열과 가열한 식재료와 비가열한 식재료의 혼합과 같은 메뉴 유형도 33.1% 수준으로 나타났다. 사용빈도가 높은 엽경채류는 시금치, 부추, 상추, 양배추, 깻잎, 양상추, 치커리, 파, 브로콜리 순서로 나타났다. 전체 식단 중 사용빈도가 높은 엽경채류 14종 370개의 데 이터에 대한 MCA 결과 PHF에 해당하는 엽경채류에는 양상추, 청경채가, Non-PHF에 해당하는 엽경채류에는 브로콜리, 양배추, 치커리, 깻잎이 주로 사용되었다. 지역별로는 중부지역에서는 마늘쫑과 시금치가, 남부지역에는 미나리 사용빈도가 상대적으로 높았다. 향후 단체급식에서 농산물의 미생물학적 안전성을 높일수 있도록 생산단계에서 오염원 차단(GAP 인증), 저감 및 세척 공정 개발, 식재료별 조리방법에 따른 메뉴 데이터 베이스화, 미생물학적 안전성이 확보되는 조리방법을 활용한 식단 개발 및 검증이 필요하다.

The purposes of this study are to develop a chitosan suspension for the carbon dioxide (CO2) concentration indication of packaged food and to investigate the changes in the CO2 indication corresponding to 2-amino-2-methylpropan- 1-ol (AMP) or 2-aminoethanol (monoethanolamine, MEA) addition. Chitosan suspension was prepared by adding chitosan (0.1, 0.2, and 0.4%) to distilled water and subsequently the pH of the suspension was adjusted to 7.0 after the addition of AMP or MEA. Changes in the pH and the turbidity of the chitosan suspension were measured over time under exposure in a normal atmosphere or 100% carbon dioxide environment. The pH of the chitosan suspension exposed at 100% carbon dioxide environment decreased rapidly up to approximately 6.3 in the initial CO2 exposure. The transmittance values of 0.1 and 0.2% chitosan suspensions with 5% AMP increased from 32 to 99% and from 19 to 86%, respectively. The addition of 5% AMP improved the visual indication performance of the chitosan suspension since it increased significantly the width of the transmittance value before and after CO2 exposure. The chitosan suspension with AMP has a potential to be used as a quality indicator of the packaged foods which produce carbon dioxide during storage and distribution.

The purpose of this study was to investigate the possibility of the use of enzymatically hydrolyzed anchovy protein (eHAP) to enhance the salty taste in salad dressing when it was applied in cooking. The intensity of the salty taste was enhanced by 5-11% when 0.1-1.5% eHAP was added. This indicates that the salty taste enhancing effect was decreased as the amount of added eHAP was increased. However, there are no significant differences between the control and samples (p>0.05). The overall preference was increased as the amount of added eHAP was increased. The preference value was the highest when 1.0% of eHAP was added, while it was slightly lower when 1.5% of eHAP was added. This may be because the content of free amino acids with a bitter taste contained in eHAP was increased. The pH value was increased as the amount of added eHAP was increased. With respect to the chromaticity, the lightness and yellowness were increased but the redness was not significantly changed when the amount of added eHAP was increased. It was found that the addition of eHAP to a salad dressing enhanced the salty taste and the preference, but the enhancement was not significant.

The purpose of this study was to investigate the possibility of the use of enzymatically hydrolyzed anchovy protein (eHAP) to enhancing of salty taste in dried pollack soup and mungbean sprout when it was applied in cooking. The salty taste enhancing effect was evaluated by comparing the dried pollack soup samples with eHAP added and the control sample containing 0.6% NaCl, and the result showed that the salty taste enhancing effect was 15%, 25%, 42%, and 46% in the samples with 0.1%, 0.5%, 1.0%, and 1.5% eHAP added, respectively. The overall acceptability was decreased as the eHAP addition was increased. In case of a bean sprout containing 0.7% NaCl, the salty taste enhancing effect was 29%, 18%, 16%, and 31% in the 0.1%, 0.5%, 1.0%, and 1.5% eHAP added, respectively. The lightness and yellowness were decreased as the eHAP addition was increased in both the dried pollack soup and the bean sprout. The pH of the dried pollack soup was decreased but the pH of the bean sprout dish was increased as the addition of eHAP was increased. The application of eHAP to food showed a significant salty taste enhancing effect, but the effect was dependent on the type of foods.

Recently, amorphous granular starch (AGS) or non-crystalline granular starch (NGS) is of great interest because it has specific physicochemical properties compared to native starches. Various approaches have been taken to prepare gelatinized starch while still maintaining its granular shape. The granular cold-water soluble starch (GCWS) can be prepared by alcoholic-alkaline treatment or by using liquid ammonia and ethanol. However, these starches exhibit significant deformation and shrinking, and chemical treatments may raise safety issues for their potential food applications. Therefore, in this study, the optimization of preparation method for amorphous granular potato starch (AGPS) was investigated using ethanol and heat treatments. Response surface methodology (RSM) and central composite design (CCD) were used to find the optimum conditions for AGPS preparation based on granule integrity and birefringence. Optimum conditions were 53.3% ethanol and 93.87oC heat treatment for 15 min. Prepared AGPS maintained its granular structure and lost birefringence, crystallinity, and DSC amylopectin melting peak, suggesting that proper AGPS could be made using optimized conditions.

The effect of temperature distribution in the retort on the degree of sterilization and the product quality was investigated. The temperature distribution in the retort chamber in an industrial scale of retort was evaluated. The target processing temperature was set to 121.1°C based on the standard sterilization process for C. botulinum. The temperature distributions at several points in the retort chamber were measured during the sterilization process, such as the heating (25°C to 121.1°C), holding (121.1°C), and cooling (121.1°C to 25°C) processes. Fo-values at different positions in the chamber were evaluated and compared. Potato cubes were used as a control sample to obtain the heat penetration curve for evaluating the degree of sterilization and the quality changes. Potato samples in cube shape (13×13×13 mm) were prepared and packed in 5 kg of retort pouch pack. A significant temperature deviation occurred during the cooling process and it significantly affected the degree of sterilization.

본 연구에서는 1차 농수산물 및 축산물의 기능성 표시광고를 제한하는 법 제도적 제한요인을 탐색하 였다. 우선 이를 위해 선행연구들을 참조하여 농수산물의 품질 및 안전성과 직결되거나 식품표시와 관 련된 법령인 양곡관리법, 축산물위생관리법, 식품위생법, 건강기능식품에 관한 법률, 시행령, 시행규칙 및 표시광고 관련 조항들을 고찰하였다. 법 조항의 적용 시 법률간 관리대상 식품 형태 및 범위의 모호 성, 규제대상 영업자 모호한 설정이 농수산물 기능성 표시광고의 주요 제한요인으로 판단된다. 또한 법 령 주관부처 별 중복규제 가능성, 행정처분 기준의 모호성 등이 확인되었다. 본 연구를 통해 농식품의 기능성 표시광고 제한요인을 도출하였으며, 이는 1차 농수산물 경쟁력 강화를 위한 정책 기초자료로 활 용될 수 있다.

The effect of drying temperature and steaming time on the browning and antioxidant activity of dried Platycodon grandiflorum was investigated. Thirteen treatment conditions were constructed using central composite face-centered design containing 5 center points. Drying temperature and steaming time (as factors) were 45-75oC and 15-45 min. According to treatment conditions, dried Platycodon grandiflorum was assessed for color characteristic, degree of browning, total polyphenol content, and DPPH and ABTS free radical scavenging (as responses). When increasing drying temperature within a given steaming time, dried Platycodon grandiflorum exhibited decreased lightness, increased redness, degree of browning, and total polyphenol contents, and enhanced antioxidant activities. Except for total polyphenol contents and antioxidant activities, steaming time within a given drying temperature exhibited similar effects to those observed in drying temperature. However, steaming time did not likely influence total polyphenol contents and revealed the opposite trends observed for the effect of drying temperature on their antioxidant activities. The overall results suggested that drying temperature was the main factor for changes in the browning and antioxidant activity of dried Platycodon grandiflorum.

Muffin was developed using blueberry powder and the shelf life of the muffin packaged in modified atmosphere was determined. Blueberry was freeze-dried and milled to prepare powder. As the concentration of blueberry powder increased from 0 to 15% (w/w), hardness and gumminess also increased (p<0.05). The Hunter L and b values of the crust and crumb of muffin decreased as the concentration increased, while their Hunter a values increased. Blueberry powder concentrations of 10 and 15% resulted in high preference in taste, texture, and overall acceptability. Blueberry muffin containing powder at 10% was packaged in modified atmosphere (MA). The optimum gas for modified atmosphere packaging (MAP) of the muffin was the mixture of carbon dioxide (CO2) and nitrogen (N2) (7:3), which improved microbial stability without altering muffin hardness. The shelf life of modified atmosphere packaged blueberry muffin was determined using the accelerated life test. The shelf life values for MA packaged blueberry muffin stored at 25 and 35°C were predicted as 21 and 5 d, respectively. Further, the Q10 values for 25- 35°C and 35-45°C were determined as 4.2 and 2.9, respectively. The MAP can preserve blueberry muffin for 3 weeks at 25°C, without the external addition of preservatives.

Recently, polycosanol products have been actively introduced into the domestic market based on their potential biological activity. The analytical procedures of polycosanol, which determine the TMS derivatives of each polycosanol with GC-FID, were presented for inspection of standards. However, the conventional procedures are not readily applicable to polycosanol emulsion which is prepared by mixing polycosanol and water together with emulsifiers because of their interferences. Therefore, the quantitative analytical procedure of polycosanol emulsion with GC-MS at selected ion monitoring mode was proposed and its validity was inspected for standardization. The analysis of polycosanol standards according to the proposed procedure showed the following values: 90.5% for reproducibility; 0.48-5.83% for the RSD; 0.000479-0.001314 μg/μL for the LOD; 0.001452-0.003983 μg/μL for the LOQ; 80.38-108.98% for accuracy; and 0.01-4.88% for the coefficients of variation. The average reproducibilities of polycosanol emulsions according to the current procedure were 87.43±4.48% and 88±2.2%, respectively, which were within the 80-120% range of the designated amounts. Therefore, it was found that the proposed analytical procedure with GC-MS at SIM mode in this study would be promising for the accurate quantitative analysis of polycosanol emulsion.

In this study, we developed and validated microanalysis methods for the determination of linear alkylbenzenesulfonate (LAS), sodium lauryl sulfate (SLS), and alpha olefin sulfonate (AOS). The conditions for the analysis of the surfactants using HPLC with FLD, RID, and ELSD detectors were investigated. The methods were validated by determining the linearity, limits of detection (LODs), limits of quantification (LOQs), recovery, precision, and accuracy. LAS analysis by FLD revealed calibration curves that were linear in the range of 10-200 mg/L for an LAS mixture. The calibration curves for C10-C13 had correlation coefficients of 0.995, 0.997, 0.996, and 0.997, respectively. SLS analysis using RID generated a linear calibration curve in the range of 10-300 mg/L. The calibration curve for SLS C12 had a correlation coefficient of 0.9994. AOS analysis using ELSD resulted in a correlation coefficient of 0.9940. For LAS, the LODs and LOQs were 0.09-0.56 and 0.30-1.87 mg/L, respectively. For SLS C12, the LOD and LOQ were 0.07 and 2.33 mg/L, respectively. For AOS C14, the LOD and LOQ were 16.55 and 21.83 mg/L, respectively. The recoveries were 97.17-98.84% for LAS C10-C14, 97.94% for SLS C12, and 96.11% for AOS C14. The established methods provide acceptable precision and accuracy. Our methods could be useful for the detection of anionic surfactants in dishwashing detergents.

This study optimized physicochemical factors for the sphericity of capsule type-fish roe analogs using equipment with double nozzles. The manufacturing process of fish roe analogs was optimized by response surface methodology with central composite design. For the optimum conditions of sphericity, sodium alginate concentration, calcium chloride concentration, agitation speed of calcium chloride solution in a reactor, and dropping height were investigated as independent variables. Soybean oil was put into the equipment through an internal nozzle, along with sodium alginate solution through an external nozzle into calcium chloride solution. The optimum conditions for the production of fish roe analogs were 1.66% (w/v) sodium alginate, 1.86% (w/v) calcium chloride, agitation speed of calcium chloride at 280 rpm, dropping height at 17 cm between the tip of the nozzle and the surface of calcium chloride solution. Consequently, the fish roe analogs showed a high sphericity of 99.8±0.77% at optimal conditions.

We developed leaching kinetics models and a convective mass transfer model for water soluble solid in jujube (Zizyphus jujube) fruits with hot water. Hot-water leaching process was conducted at 90°C for 100 min. The ratio of solvent (volume, mL) to solid (mass, g) was set to varied ratios of 1:6, 1:8, 1:10, and 1:12.5. The mass transfer coefficients (k) were determined based on the 1st- and 2nd-order kinetic models. The amount of solvent influenced the extraction rate and yield. A higher extraction rate and a higher yield were obtained from a lower solvent to solute ratio (1:6, 1:8, 1:10) compared to high ratio (1:12.5). This implies that an appropriate amount of solvent (i.e., water) is necessary to conduct the extraction process of jujube fruit.

The reduction of the content of sodium chloride in noodle was studied to prevent the problems related to excessive sodium intake, such as high blood pressure and cardiovascular diseases. This study investigated the effect of hydrolyzed anchovy products (HAP) on the salty taste of cooked noodle. The experiment was set up to determine the general properties of dough in dried and cooked noodles, with a sensory test used while increasing the concentration of HAP at same salt contents. There was no significant difference in water binding capacity, solubility, swelling power, lightness, and cooking properties. The redness, yellowness, and hardness were increased upon increasing the contents of HAP. From the sensory evaluation, cooked noodle with added HAP had a high score in appearance, color, texture, and flavor. Moreover, 50% and 75% HAP showed higher values than the control item in saltiness, preference of saltiness, and overall acceptance. Consequently, the HAP could have an effect on dried noodles or cooked noodles as a salty taste enhancer without changing the noodle’s properties.

Brown rice, barley, and buckwheat contain a variety of functional ingredients, such as vitamins, minerals, and dietary fibers. Germination is an effective technique for improving the digestion and absorption of the constituents in grains. In addition, germination could modify the chemical properties of grains and increase the content of biologically active substances. Many studies have reported that the intake of grains or germinated grains could ameliorate blood glucose level, stimulate the immune system, lower blood pressure, and result in anti-obesity effect. Therefore, grains and germinated grains are being utilized in a variety of functional products. The present study reviews the primary components and bio-functionalities of grains (brown rice, barley, and buckwheat) and germinated grains (germinated brown rice). The development of grain products for promoting health functional properties is also discussed.

본 연구에서는 육상양식장에서 배출되는 부산물의 자원화 방안으로 뱀장어 양식장에서 배출되는 고형오 물에 미강, 탈지대두박과 같은 식품 부산물을 첨가하여 유산균, 황국균, 고초균을 단계적으로 접종하여 발 효시킨 발효사료의 성분 변화를 측정하였다. 발효사료의 제조 단계별 수분함량은 유산균을 이용한 1단 발 효물 14.6%, 황국균과 고초균을 이용한 2단, 3단 발효물은 각각 33.0%와 34.0% 였다. pH는 1단 발효물 에서는 유산균에 의한 젖산의 분비로 인해 5.38로 나타났으며 2단, 3단 발효물은 각 5.66과 7.26으로 측 정되어 pH가 상승하는 것으로 나타났다. 피트산의 함량은 1단 발효물은 0.126g/100g, 2단 발효물은 0.004g/100g, 3단 발효물은 0.093g/100g으로 나타나는 것을 확인 할 수 있었다. 질소 함량을 측정한 결 과 아미노태질소 함량은 2단 발효물이 1226.37mg%로 높았고, 3단 발효물에서 710.18mg%로 다소 감소 하였으며, 암모니아태질소 함량은 1단 발효물 0.988mg/kg에서 3단 발효물 1.502mg/kg로 증가되었다. 총질소 함량은 1단 발효물 2.78%와 2단 발효물 4.08% 그리고 3단 발효물 4.85%로 증가되었다. 3가지 미생물로 연속적인 발효가 진행될수록 피트산은 감소하고 단백질 분해율이 높아지는 경향이 나타났고 3 단 발효에 의해 저분자 질소성분 함량이 증가한 것으로 미루어 소화 흡수성이 높은 사료화가 진행됨을 추측할 수 있다.

식품에서 대장균군을 효율적으로 분리하기 위해 대장균군 선택배지 5종: Chromocult Chromocult coliform agar (Merck), Hicrome coliform agar (Sigma), CHROMagar ECC, Brilliance E.coli/coliform medium (OXOID), endo agar (Merck)을 선별하여 식품 분리 대장균군 83종 및 표준균주 21종에 대해서 민감도와 특이도를 분석한 결과 Chromocult coliform agar와 HICrome coliform agar에서 94%도 민감도가 가장 높았으며, Brilliance E.coli/coliform medium는 93%, CHROMagar ECC는 92%, ENDO agar는 74%의 민감도를 나타냈다. Chromocult coliform agar와 HICrome coliform agar는 대장균군의 회수율도 높았다. 그러므로 Chromocult coliform agar와 HICrome coliform agar는 대장균군을 분리하는데 가장 효율적인 배지로 생각된다.

본 연구는 어린이 기호식품에 대한 초등학생들의 인식과 섭취 실태/행동을 알아보고, 또 성별 및 학년과의 연관성을 알아보고자 수행되었다. 일개 도시의 13개 초등학교 주변에서 무작위 추출된 초등학생 306명(남 188명, 여 118명)에 대하여 직접면담법에 의한 설문조사를 수행하였다. 전체적으로 20% 이상의 학생이 어린이 기호식품에 대한 지식이 없었으며 또 어린이 기호식품에 대한 정보원이 없었다. 또한 60% 이상의 학생이 1주일에 1~2회 이상 학교주변 어린이 기호식품 판매업소를 이용하는 것으로 나타났다. 어린이 기호식품에 대한 정보 습득 경로로는 학교가 37%로 가장 많고, 다음으로 가정 16%이었다. 어린이 기호식품에 대한 의식 및 행동을 나타내는 요인들 중에서 지식과 정보 습득 경로는 성별과 유의한 연관성이 있었다(p < 0.05). 또 지식, 정보 습득 경로, 태도, 섭취 이유에 있어 학년과 유의한 연관성이 있었다(p < 0.05). 남자 어린이가 여자 어린이보다 지식 수준은 높았으나(p < 0.05), 태도는 낮고 또 섭취 빈도가 높았다. 학년이 높을수록 어린이 기호식품에 대한 지식과 태도 수준이 높아지고 다양한 정보 습득 경로를 가졌으나(p < 0.05), 또한 섭취 빈도가 높았다. 이러한 결과는 조사대상자의 지식과 태도/행동의 불일치를 나타낸다. 그러므로 학생들의 주요 정보 습득 경로인 학교급식 프로그램과 학교 및 가정에서의 식품위생/안전교육이 더욱 향상되어야 함을 나타낸다. 더불어 학교주변에서 불량식품에 대한 규제도 강화되어야 할 것이다.

본 연구에서는 최대한 인삼의 외형을 원형삼 형태의 홍삼 과 유사하게 유지하면서도 기능성은 증진시킬 수 있는 신속 한 고온고압 처리 공정을 확립하기 위하여 다양한 고온고압 처리공정 조건에 따른 이화학적 성분 특성 및 항산화 활성 변화를 살펴보았다. 산성다당체 및 홍삼 특유의 진세노사이 드 Rh1, Rg2, Rg3의 함량은 140℃, 3 kg/cm2의 고온고압 처리 조건에서 가장 높은 반면, 총 페놀 화합물 및 말톨 함량은 156℃, 5 kg/cm2의 고온고압 처리조건에서 가장 높았다. 그러 나 홍삼의 증자 처리 시 156℃, 5 kg/cm2의 처리조건에서는 시 료가 터지거나 외형의 변형이 심하기 때문에 140℃, 3 kg/cm2를 최적 온도 및 압력으로 설정하였다. 한편, 증자 시간이 증 가함에 따라 총 페놀 화합물, 말톨 및 흑삼특이 진세노사이드 함량은 지속적으로 증가하는 경향을 나타내었으나, 20분간 처리한 군의 외형이 기존의 홍삼과 가장 유사한 외관을 나타 냈으므로, 140℃, 3 kg/cm2에서 20분 동안 증자 처리하는 것 을 본 실험의 최적 조건으로 설정하였다. 최종적으로 이러한 최적조건을 통해 제조된 홍삼의 항산화 효능을 분석한 결과, 시중에서 판매되는 백삼, 홍삼 및 흑삼과 비교하여 높은 항산 화 성분 및 항산화 활성을 나타냈다. 따라서 본 연구를 통해 확립된 고온고압 처리를 통한 신규홍삼 제조기술은 그 형태 가 기존의 홍삼 제품과 유사하면서도 공정이 신속하고, 품질 은 흑삼과 비슷한 고기능성 신규 인삼제품 개발 시 응용 가능 한 공정으로 사료된다.

Macrophages play a pivotal role in the innate and adaptive immune systems. This study investigated the immuno-modulatory activities of polysaccharides separated from Chrysanthemum zawadskii var. latilobum (CZPS) in macrophages. Polysaccharides from Chrysanthemum zawadskii var. latilobum were extracted by the ethanol precipitation method. RAW 264.7 mouse macrophage cell line was treated with CZPS (4 to 128 μg/mL), and there was no cytotoxicity at a dose below 32 μg/mL. The levels of nitric oxide (NO) and pro-inflammatory cytokines (tumor necrosis factor (TNF)-α and interleukin (IL)-6, IL-1β) production in the CZPS treated group (32 μg/mL) were 6.5±0.12 μM (NO), 1252.8±79.85 (TNF-α), 305.4±29.41 (IL-6), and 683.3±59.71 (IL-1β), respectively, and they were significantly increased when compared to the control group; 2.2±0.03 μM (NO), 452.3±38.34 (TNF-α), 31.7±5.75 (IL-6), and 184.1±11.52 (IL-1β). Additionally, protein expression of inducible nitric oxide synthase (iNOS) and phosphorylation of MAPKs and NF-κB expression were significantly increased upon CZPS treatment. Therefore, these results indicated that polysaccharides separated from Chrysanthemum zawadskii var. latilobum (CZPS) may have a potential immunomodulatory activity in macrophages through MAPKs and NF-κB signaling, and this information is useful for the development of immune enhancing adjuvant materials using a natural ingredient.