One species of the genus Coleopius (Hymenoptera: Braconidae: opiinae) are reported in this study. The genus Coleopius is a small braconid group distributed in the Palaearctic and Oriental regions, which has three species in the korea in current. In this study, Coleopioides postpectalis Li and van Achterberg is recognized for the first time in South Korea, which had been recently recorded from China. Descriptions, diagnosis distribution and illustration are provided
The genus Gonioctena Chevrolat, 1836 is one of the largest genera of the subfamily Chrysomelinae with approximately 110 valid species in nine subgenera distributed in the Holarctic and Oriental regions. The Gonioctena mauroi species-group of the subgenus Brachyphytodecta Bechyně, 1948 is proposed to include four species: Gonioctena mauroi Cho & Borowiec, 2016 (Vietnam), G. medvedevi Cho & Borowiec, 2016 (Vietnam), G. kuatunensis sp. nov. (China) and G. longshengensis sp. nov. (China). They are characterized by the presence of setae on the aedeagus, which is unique within the subgenus. A key to the species, habitus photographs, illustrations of diagnostic characters and a distribution map are provided. Ovoviviparity is newly recorded in G. longshengensis sp. nov.
곤충병원균인 Pseudomonas entomophila를 Riptortus pedestris의 체내에 주사하였을 때, 곤충을 사멸시키는 효과를 나타내는 물질을 찾기 위하여, P. entomophila를 배양한 배양액을 이온교환 크로마토그래피 및 크기배제 크로마토그래 피를 통해 살충 효과를 나타내는 물질을 정제 하였다. 그 결과 R. pedestris에 대해 살충효과를 보이는 독성 물질은 metalloproteinase의 일종인 AprA 단백질인 것으로 나타났다. 정제 된 AprA를 R. pedestris에 주사하였을 때, 곤충이 매우 빠른 속도로 사멸하는 양상을 보였다. AprA 단백질이 어떠한 작용 기전으로 R. pedestris에 독성 효과를 보이는지 알기 위하여, 정제 된 AprA를 R. pedestris의 체내로 주사한 후 hemocyte (혈구)의 숫자를 조사한 결과, AprA를 주사 한 직후에는 혈구의 숫자가 증가하였으나, 1시간 이후부터는 혈구의 수가 급격히 감소하기 시작하였고, 결국은 모든 곤충이 사멸하였다. 본 연구진은 AprA가 R. pedestris의 면역반응을 어떻게 억제하는지 알기 위해, AprA가 R. pedestris의 혈구를 제거하는 기작을 보고하고자 한다.
In order to establish symbiotic host-bacterial relationships, symbionts in insects evolved a mechanism to overcome host immune responses. Here we provide the resistance of symbiotic bacteria on the insect immune system. As a result, through the transposon mutagenesis, we found a salivary gland (SG) susceptible mutant. The disrupted gene was identified as nlpB involved in lipoprotein synthesis. The nlpB, bla double deletion mutant was sensitive to SG like nlpB-Tn5 inserted mutant. This mutant increases outer membrane permeability. It provides an explanation for SG susceptibility, because the antimicrobial peptide in SG would be able to translocate across the outer membrane more easily than in the wild type. These results indicate that nlpB and bla are likely to be important factors in terms of determining resistance against SG of Riptortus that is connected with the successful colonization of the Riptortus midgut.
R. pedestris의 중장인 M4에 Burkholderia 균이 공생하고 있으며, 이 공생균은 숙주의 면역에 이로운 영향을 준다. 본 연구실에서 외부 병원체의 침입에 따른 면역 반응으로 항균활성을 가지는 3 종류의 항균 펩타이드를 정제하였다. 그 중에서 Rip-thanatin은 18개의 아미노산으로 이루어져 있으며, 2개의 시스테인에 이황화 결합이 있는 펩타이드로, R. pedestris에 많은 수의 유도체가 존재하고 있다. 이 Rip-thanatin은 병원체를 숙주에 주사하였을 때 공생균을 가지는 곤충의 M4 중장에서 공생균을 가지지 않는 곤충보다 유전자 발현양이 유의적으로 높아지는 사실을 관찰하였다. 따라서, Rip-thanatin이 중장의 면역반응에서 중요한 역할을 하며, 이는 공생균의 유무에 의해 영향을 받는 것으로 규명되었다. 본 연구진은, 숙주의 면역반응에 있어서 Rip-thanatin의 중요성을 규명하기 위해, 다양한 병원체에 대한 Rip-thanatin의 유전자 발현 정도를 관찰하고, 여러 가지 Rip-thanatin의 유도체를 합성하여 항균 활성과 Rip-thanatin의 구조적 상관관계를 규명하고자 한다.
RNA interference (RNAi) has been widely adopted as a primary reverse genetic tool to determine the physiological function of genes of interest. Nevertheless, the lack of optimized RNA delivery method has been a major obstacle for non-model organisms, such as Cimex lectularis. In this study, we have established a RNAi protocol for the silencing of C. lectularis salivary gland-specific cholinesterase (SChE) gene based on micro-injection of double stranded RNA (dsRNA). An aliquot (20 nl) of dsRNA solution (4.5 ng/nl) was injected to body cavity through the arthrodial membrane between metathoracic coxa and sternum of adult females. Observed mortality was less than 5% and at 6-day post injection, while the gene silencing efficiency reached 97~99% at 2-6 day post injection. This result demonstrates the efficacy of injection RNAi via the arthrodial membrane in C. lectularius.
Abaecin is a proline-rich anti-bacterial peptide which is known to have high activity against Gram-negative bacteria and it is originally isolated from honeybee Apis mellifera. Since the anti-bacterial peptides including abaecin were identified in the hemolymph of A. mellifera infected with Gram-negative bacteria Escherichia coli, this result implies that honeybees express abaecin for defense against natural infection. Anti-microbial peptides including abaecin were also identified in the adult hemolymph of bumblebees. To investigate pharmacological and toxicological properties of abaecin from Bombus ussurensis, biological activities were evaluated by conducting anti-tumor, anti-microbial and cytotoxic activity assays. Abaecin exhibited significantly high anti-tumor activity against ovarian tumor cells SK-OV-3 and NIH; OVCAR-3 at 100 μM after 72 h treatment. Interestingly, abaecin showed none of anti-microbial activity against Gram-negative and Gram-positive bacteria. Since abaecin exhibited extremely low level of hemolytic activity against human erythrocytes, it may serve as a good model peptide for studying its potential as a selective anti-tumor drug.
To identify genes that commonly respond to the treatment of different insecticides, 3rd instar larvae of the diamondback moth, Plutella xylostella, were treated with sublethal doses (<LC10) of chlorantraniliprole, cypermethrin, dinotefuran, indoxacarb and spinosad via leaf dipping. Then, transcriptomic profiles of treated larvae were compared with that of untreated control. A total of 117,181 transcripts in average with a mean length of 662 bp were generated by de novo assembly, of which 35,329 transcripts were annotated. Among them, 207, 153, 336, 360, and 262 transcripts were determined to be up-regulated whereas 117, 47, 92, 115, and 81 genes were down-regulated following treatments with chlorantraniliprole, cypermethrin, dinotefuran, indoxacarb and spinosad, respectively. Finally, with the criteria of >10 X fold change (FC) and p < 0.05 or >4 X FC, p < 0.05 and q < 0.2, the genes commonly over-transcribed in all treated insects were selected and their over-transcription levels were confirmed by quantitative PCR. These commonly responding genes included three cytochrome P450 genes (Cyp303a1, Cyp6a20 and CYP9E2), three cuticle protein genes (LM-8, LM-19 and TM-A3A), lavesin-1, acyl-CoA D11 desaturase, glucose dehydrogenase, nose resistant to fluxetine protein 6, chorion peroxidase and protein yellow. As the five test insecticides have distinct structure and mode of action, the genes identified in this study were suggested to be involved in general chemical defense at the initial stage of intoxication. Their possible roles in tolerance and resistance development were further discussed.
Genus Trimorus are egg parasitoids of Carabid beetles. They are comprised of two subgenus (Trimorus, Neotrimorus), Neotrimorus are composed of 3 species. One of them Trimorus (Neotrimorus) ferrari was described by Veenakumari & Rajmohana from the Andaman islands India, but only male is determined. For the first time, female Trimorus (Neotrimorus) ferrari Veenakumari & Rajmohana, 2013 is collected from Myanmar. Herein, description and photograph of female T. (Neotrimorus) ferrari are provided, also redescription and photograph of male are included.
In this paper, two species of Mirine plant bug, Polymerus amurensis Kerzhner, 1988 and Rhabdomiris striatellus Fabricus, 1794 are newly recorded from Korean peninsula. The dorsal habitus and genital structure of each species were provided. Also, figure of related species, P. pekinensis Horvath, 1901 and R. pulcherrimus Lindberg, 1934 were also documented to aid in the identifications of newly recorded species respectively.
Recently, the existence of non-neuronal, soluble AChEs with non-classical functions, such as stress response and chemical defense, has been reported in both vertebrates and invertebrates. With this in mind, it is intriguing to hypothesize that fat body is a main tissue to express non-neuronal AChE at least in some insects. As an initial step for the systematic approach to investigate the distribution of non-neuronal AChEs in insect fat body and to elucidate their physiological functions, we have selected 12 different insect species across different orders and isolated fat body tissues from them. Then, the presence or absence of AChE and its solubility nature were determined by native polyacrylamide gel electrophoresis in conjunction with western blot analysis insect-specific AChE1 and 2 antibodies. Among 12 insects examined, soluble AChE1 was determined to be expressed in fat bodies of insects involving honey bees, brown plant hoppers, dynastid beetles, lice, etc, AChE2 in fruit flies, bed bugs, mealworm beetles. However, no AChE was detected in fat bodies of the remaining two species American cockroaches and dragonflies Our findings clearly show that AChE is widely distributed in the fat body tissue of diverse insect species. More extensive investigation on in a wider variety of insect species would be necessary to deduce the evolutionary origin of fat body-specific AChE, which would be the ancestor of AChE with non-neuronal function.
안동⋅임하 유입지천의 저서성대형무척추동물의 군집안정성 분석을 위해 4개 지점(St.1, St.2, St.3, St.4) 선정 후 2016년 5월부터 9월까지 총 4회 조사를 실시하였다. 총 5문 8강 18목 56과 108종 53,368.8개체가 조사되었고, 안동호(St.1, St.2)는 총 4문 6강 14목 49과 82종 31,253.9개체, 임하호(St.3, St.4)는 총 5문 7강 17목 50과 83종 22,114.9개체 가 조사되었다. 전체 출현 분류군 중 하루살이목이 안동호 17종(20.7%), 임하호 16종(19.3%)으로 가장 많은 종수비율을 차지하였다. 출현 저서 동물을 대상으로 군집분석을 실시한 결과 안동호의 평균 우점도지수는(DI) 0.66 평균 다양도지 수(H') 2.39 평균 풍부도지수(R1) 2.47 평균 균등도지수(J') 0.55로 나타났다. 임하호의 평균 우점도지수는 0.59, 평균 다양도지수 2.69, 평균 풍부도지수 2.61, 평균 균등도지수 0.62로 나타났다. 군집안정성분석 결과 두 수계 모두‘I’그룹(저 항력↑, 회복력↑)에 속하는 종들의 출현율이 안동호 66.2%, 임하호 62.5%로 가장 높게 나타났다.
Chinese longhorned beetle (CLB; Anoplophora chinensis) and Asian longhorned beetle (ALB; A. glabripennis) were accidentally introduced into the US from the Asia back in 90s’ and have been causing economic damage on hardwood species in the US. An international cooperative team made field surveys in Korea to find parasitoids of CLB and ALB using sentinel logs that were infested with eggs of the two cerambycids.. As a result, two parasitic wasps of Anopolophora species were newly recognised from Korea: Aprostocetus anoplophorae Delvare (Eulophidae) as an egg parasitoid of CLB and Leluthia honshuensis Belokobylskij & Maeto (Braconidae) as a larval parasitoid of ALB. Identification was made using both morphological and molecular data. Morphological information was provided along with images of developmental stages and adults.
안동댐 내 저수지 및 유입 하천 6개 지점에 대하여 2016년 7월과 9월, 총 2회에 걸쳐 저서성 대형무척추동물 조사를 수행하였다. 분류군별 출현현황은 저수지 수변부(L1, L2, L3)와 유입 하천(S1, S2, S3)에서 각각 총 9목 31종, 14목 79종이 출현하였다. 군집지수 분석결과, 평균 다양도지수(H')와 균등도지수(J')는 S1에서 각각 3.04와 0.73으로 가장 높았으며, 평균 풍부도지수(R')와 우점도지수(DI)는 각각 S2(2.84), L3(0.98)에서 가장 높게 나타났다. 섭식기능군(FFG) 분석결과, 종수 점유율은 저수지에서 P(Predator)가 41.9%, 유입 하천에서 GC(Gathering Collector)가 30.4%로 가장 높게 나타났다. 서식기능군(FHG) 분석결과, 비교적 유수역을 선호하는 CL(Clinger)의 종수점유율이 유입 하천에서 43.0%로 저수지의 32.3%보다 더 높게 나타났다.
낙동강유역의 자연형 습지와 인공저수지, 인공댐호를 대표하는 10개 호소를 선정하여 2016년 4월과 8월, 총 2회에 걸쳐 각 호소내의 저서성대형무척추동물 조사를 수행하였다. 분류군별 출현현황으로 총 18목 58과 96종이 출현하였다. 군집지수 분석결과, 평균 우점도 지수(DI)는 용연지에서 0.92로 가장 높았으며, 다양도지수(H')는 주남지 (2.41), 풍부도지수(R')는 우포 늪(2.36), 균등도지수(J')는 선암지(0.75)가 가장 높게 나타났다. 섭식기능군은(FFG)은 P(Predator)의 종수 비율이 40.6%로 가장 높게 나타났으며, 서식기능군(FHG)은 CL(Clinger)의 종수 비율이 27.1%로 가장 높게 나타났다. 유사도 분석 결과, 우포늪과 주남저수지의 유사도가 60.9%로 가장 높은 것으로 나타났고, 용연지와 우포늪의 유사도(15.2%)가 가장 낮은 것으로 나타났다.
Filixungulia, a small genus within the subfamily Allantinae (Symphyta: Tenthredinidae), consists of three species distributed only in Northern China: F. alboclypea Wei, 1997; F. crassitarsata Wei, 1997; F. cylindrica Wei, 2003. It is close to Asiemphytus Malaise and Macremphytus MacGillivray but is separated by the following characteristics: claws simple and very slender; basal lobe and inner tooth absent. Up to date, Filixungulia has not yet been recorded out of China. As a result of the present study, the genus Filixungulia is newly recognized from South Korea with two species, F. alboclypea and F. cylindrica. This discovery extends the range of the genus Filixungulia, which was previously known as a Chinese endemic genus. Also, a key, diagnosis and photographs of each species are provided.
The subfamily Pimplinae currently include 1,737 worldwide species in 77 genera. 57 species in 18 genera have been recorded from South Korea. Among them, the genus Scambus is a moderate sized group in the subfamily Pimplinae with 152 described species from Nearctic, Neotropical, Oriental and Palaearctic regions. The taxonomic study of the South Korean Scambus was initiated by Uchida (1928), who reported one species, Scambus calobatus (Gravenhorst, 1829). The genus Tromatobia is small group in this subfamily with 33 species from Afrotropical, Austrian, Nearctic, Neotropical, Oriental and Palaearctic regions. This genus was newly recorded from South Korea for the first time. In this study, we report seven unrecorded South Korean species, Scambus nigricans (Thomson, 1877), Scambus vesicarius (Ratzeburg, 1844), Tromatobia flavistellata Uchida & Momoi, 1957, Tromatobia nipponica Uchida, 1928, Tromatobia ornata (Gravenhorst, 1829), Tromatobia ovivora (Boheman, 1821) and Tromatobia variabilis (Holmgren, 1856).
Leucospidae Walker are a relatively small family in Chalcidoidea, and they consist of four genera and about 140 species in worldwide. In neighboring countries, 12 species are known from China and four species are known from Japan, but only one species is known from South Korea by Paik (1974). In this study, Leucospis yasumatsui Habu is reported for the frist time from South Korea. A key to South Korean species of the genus Leucospis Fabricius, diagnosis, photos of characteristics and distribution from South Korea are provided.
Monodontomerus Westwood is widely distributed genus in Torymidae Walker, and it consists of 41 species with one fossil species. In Palearctic region, 18 species are recorded, each 5 species are known from Japan and China, and 8 species from Russia. However, in South Korea, only two species are known as follows: Monodontomerus dentipes (Dalman) and M. minor (Ratzeburg). In this study, four species are newly reported and a key to South Korean species of the genus Monodontomerus Westwood, diagnosis, photos of characteristics are provided.
Pteromalinae Dalman, 1820 are the largest subfamily in Pteromalidae and currently include 2,073 extant species of 314 genera. This group is probably difficult to define taxonomically. Members of this subfamily are endoparasitoids of Lepidoptera, Diptera and Coleoptera larvae and pupae. Up to date, only 46 species of 30 genera belonging to five subfamilies have been recorded from South Korea. As a result of this study, we reported 25 unrecorded species of 13 genera for the first time from South Korea. Therefore, 71 species of 43 genera in Pteromalinae are recorded from South Korea.