In order to investigate the genotypic variations of Zn accumulation in rice plant, thirty-five rice cultivars of different cultivar groups (temperate japonica, tropical japonica, tongil, and indica) were irrigated with irrigation water containing 2 ppm Zn throughout all growth season in a field experiments in 2007. At harvest, thirty-five rice cultivars showed large difference in Zn concentrations in root, shoot, straw, grain, chaff, brown rice, rice bran, and polished rice. Zn concentrations in polished rice ranged from 5.15 to 21.24 mg/kg. The total Zn accumulation in shoot varied from 12.18 to 54.84 mg/m2. Two japonica rice cultivar groups (temperate japonica, and tropical japonica) presented the higher Zn concentration in shoot compared to indica and Tongil cultivar groups. The large genotypic variation suggested the possibility of breeding low Zn accumulating rice varieties.
Assessment of the extent of genetic variability within a cultivated crop has important consequences in plant breeding and conservation of genetic resources. Random amplified polymorphic DNA (RAPD) markers were used to produce barley genomic DNA fingerprints. The aim of this work was to investigate the efficacy of polymorphism contained in such fingerprints on disclosing genetic relationships among 26 barley genotypes including advanced lines and released cultivars. Among the 21 primers the percentage of polymorphic bands ranged from 33% (OPG-07) to 83% (OPG-02), showing a high degree of molecular variation. The highest genetic similarity was observed between genotypes Karan-521 and Karan-741(58%), and the lowest genetic similarity was observed between the cultivars Lakhan and C 138 (3.7%). The similarity matrix by the UPGMA algorithm delineated the above genotypes into four major clusters. The results demonstrate the utility of RAPD markers in differentiating Indian barley varieties. KT and SJY were supported by 2008 National Agricultural Germplasm Management System Establishment Program.
BC1F7 RILs were cultivated and harvested in field. Two methods were adopted to classify ecotype of the RILs. First method was based on the seed length, width, length/width ratio, phenol reaction of the seed, and response to potassium chlorate of young seedling. Second was the classification using the polymorphism in SSR analysis. The results of UPGMA cluster analysis indicated that 168 RILs were classified into two ecotypes, 32 Japonica and 136 Tongil-type lines when first method was adopted. When second method was adopted, 35 and 99 lines among 134 RILs tested were belonged to Japonica and Tongil-type, respectively. The RILs belonged to same ecotype in both methods was 65.6% in Japonica, 91.9% in Tongil-type and 83.6 % in overall. The parents and BC1F7 RILs showed polymorphism in 20 among 21 RM primers used in SSR analysis. The proportion showing band pattern of each primer corresponded with ecotypes grouped by two methods ranged 29.7 to 99.1%. RM131, RM124, RM567, RM559, and RM348, which are located on chromosome 4, showed high proportion of correspondence over 94%. It was suggested that they would be used as molecular markers for rice ecotype classification. Japonica RILs grouped by two methods showed shorter grain length, no seed response to phenol solution and higher resistance to potassium chlorate solution in seedling stage compared with those of Tongil-type lines.
Most soybean cultivars have ovate leaflets, although a few cultivars have narrow or lanceolate leaflets. Narrow leaflet cultivars tend to have more seeds produced per pod than ovate leaflet cultivars, suggesting that the narrow leaflet trait is tightly linked to or cosegregating with the trait controlling number of seeds produced per pod (nspp). The objective of this study was to construct a high resolution map of a chromosomal region controlling narrow leaflet trait and nspp in soybean. A BC3F2 population from a cross between 'Sowonkong' and 'V94-5152' was used. Sowonkong have narrow leaflet and 4-seeded pod and V94-5152 have ovate leaflet and do not have 4-seeded pod. The plants of F2 populations showed a segregation ratio of 3:1 of ovate leaflet to narrow leaflet and then leaflet genotypes were obtained from F2:3 population of each F2 individual. The narrow leaflet-containing plants showed Sowonkong-like pod trait and the ovate leaflet-containing plants V94-5152-like pod trait. The results suggested that, in our mapping population, a single gene controls inheritance of the narrow leaflet character and the narrow leaflet trait is tightly linked to or co-segregating with the trait controlling nspp. Subsequently, we mapped the narrow leaflet locus near Sat_105, Satt270 and SM315 on soybean linkage group I that corresponds with the previously known ln locus. Work is ongoing to construct a fine molecular genetic linkage map on this MLG I region and to find a linkage relationship between ln and nspp. Our results should facilitate further elucidation for the relationship between ln/nspp and yield.
The objectives of this study were to collect and maintain wild barley accessions and to analyze their phylogenetic relationships among the accessions. Sixty wild barley accessions were collected from Syria (four accessions), Jordan (eight), Afghanistan (six), Iraq (three), Iran (four), Turkey (two), Pakistan (one), Tajikistan (one), Azerbaijan (one), Palestine (fifteen), and unknown area (fifteen). Seeds of the three accessions failed to germinate. Thirty-seven random amplified polymorphic DNA (RAPD) were generated and the phylogenetic relationship of the accessions was analyzed. Large genetic diversity existed among the collections and the collections were classified into 4 groups at a similarity coefficient of about 0.7698. Significant variations were observed within the collections from the same geographical location. The collections have sufficient genetic and morphological diversity for the genetic and phenotyphic analyses of traits associated with tolerance to abiotic stresses such as salt and drought.
Major agronomic and morphological traits of Korean land races of cultivated barley were analyzed. Three hundred sixty-three Korean land races, 69 hull-less and 295 hulled barely, were cultivated at the field of National Honam Agricultural Research Institute at Iksan, Korea. Olbori and Saessalbori were used as control varieties for hulled and hull-less barley, respectively. Traits such as heading date, maturity, plant height and flag leaf length and width were analyzed. Heading date and maturity were variable 22 and 22 days, respectively, among the land races tested. Plant height of land races corresponded to 74 to 174% of that of the control varieties. Flag leaf length and width varied from 87% to 314% and from 57 to 233%, respectively, of that of the control varieties. This result indicates that genetic diversity for agronomic and morphological traits are significantly broad among Korean land races and they could provide valuable genes to improve current elite lines.
콩의 주요 Allergy 단백질원은 α-subunit, Gly m bd 28K 및 Gly m bd 30K로 알려지고 있으며, 콩 유전자원에 대한 저 알러지 자원을 발굴하기위하여 α-subunit(Gly m bd 60K)는 SDS-PAGE로, Gly m bd 28K와 Gly m bd 30K는 Antibody를 작성하여 검정하였다. α-subunit는 1500개의 자원을 검정한 결과 한국자원 CS1151와 CS79, 일본자원 CS735, CS737 및 중국자원 CS1733의 5개 결실 자원을 발굴하였다. Gly m bd 28K는 Antibody를 작성하였으며 2D-electrophoresis로 Antibody를 확인한 결과, Gly m bd 28K는 4개의 Spot으로 이루어진 단백질로 구성되어 있음을 확인하였다. Gly m bd 28K와 Gly m bd 30K의 저하자원을 발굴하기 위하여 Antibody를 1/10000농도로 적정하여 Western-Blot실시하여 800개의 유전자원을 검정한 결과 Gly m bd 28K는 CS899와 CS909가 저 알러지 자원으로, Gly m bd 30K는 PI567476가 저 알러지 자원으로 확인되었다.
한국의 야생콩 40종, 육성품종 21품종 및 중국길림성 야생콩 36종의 97종에 대하여 다형성이 높은SSR 9개 마커를 사용하여 유전적 다양성과 유연관계를 분석하였다. 전체 97종에서 대립인자는 총291개로 확인되었고, 그 범위는 21~39개였으며 평균 32.3개였다. 또한 한국 야생콩의 대립인자 평균치는 15.2개, 한국 육성품종은 11.3개, 중국 길림성의 야생콩은 12.7개였다. 전체 97종의 유전적 다양성(PIC) 값의 범위는 0.960(satt185)~0.896(satt141)이었고, 평균 유전적 다양성(PIC) 값은 0.937이었다. 또한 유전적다양성은 한국 야생콩은 0.871, 한국 육성품종은 0.858, 중국 야생콩은 0.821으로서 큰 차이가 없었다. 유전적 거리에 의해 7그룹으로 분류되었으며, 1그룹에는 한국 야생콩, 2그룹에는 중국 야생콩, 5그룹에 한국 육성품종만이 모두 속해 있었다. 3과 4그룹에는 중국 야생콩이 주로 속하였으나 한국 야생콩이 각 그룹에2종씩 속하였고, 6과 7그룹은 한국 야생콩이 주로 속하였으나 중국 야생콩이 각 그룹에1 종씩 속하였다. 한국 야생콩과 육성품종 및 중국 길림성 야생콩 간에는 유전적다양성이 큰 차이가 없었으나 유연관계는 각 집단 간에 큰 차이가 있었으며 일부 한국 야생콩과 중국길림성 야생콩의 몇 종이 유연관계가 가까웠다.
국내 육성 콩 10품종의 각 부위별(자엽, 종피 및 배) 지방함량과 지방산 조성의 품종간 차이를 검토하였다. 지방함량은 자엽>배>종피 순서로 많았으며 각 부위별 평균 지방 함량은 자엽이 18.3%, 배가 8.9%, 종피가 1.4%이었다. 각 부위별 지방 함량은 자엽은 대망콩이 가장 높았고 서남콩이 가장 낮았으며, 배는 대망콩이 가장 높았고 호장콩이 가장 낮았다. 종피는 다장콩이 가장 높았고 새별콩이 가장 낮았다.
지방산 조성은 각 부위별로 자엽은 C18:2>C18:1>C16:0>C18:3>C18:0의 순서로, 종피는 C18:2>C16:0>C18:3>C18:1>C18:0의 순서로, 및 배는 C18:2>C16:0>C18:3>C18:0>C18:1의 순서로 높았다. 각 부위별로 linoleic/linolenic acid의 비율은 자엽은 명주나물콩이 가장 높았고 대망콩이 가장 낮았으며, 종피는 진미콩이 가장 높았고 대풍콩이 가장 낮았다. 배는 다장콩이 가장 높았고 대풍콩이 가장 낮았다.
Most gene functions of biochemical pathways were still unexplored, especially interactions of constituent genes. We attempted to uncover interaction network of biochemical pathways via a survey of co-expression clusters, which we have constructed from the NCBI GEO database, and then to define key genes of networks with expression correlations between members. Top 20 pathways with high numbers of individual genes were retrieved from 178 pathways. One pathway, ‘removal of superoxide radicals’ was excluded for further study, evidencing somewhat low degree (16%, 13 out of 79 genes) of mapped probes. We employed expression correlations of random pairs of 1,000 randomly selected genes for determining a cut off r-value for gene networks. Numbers of interactions with a significant expression correlation values between members might evidence that “hub genes” play key roles among a given pathway genes. For example most interactive pathway, ‘tRNA charging pathway’, that is composed of 60 probes corresponding to genes showed 264 positive significant interactions between members of 47 genes while 5 negative interactions between members of 7 genes., evidencing ‘Os10g26050’ (methionyl-tRNA synthetase) gene with highest interactions is suggestive of a hub gene. These findings might provide some clues on evolutionary fate of co-expression genes including each of biochemical pathways, e.g. convergent evolution
Genetic diversity plays a great role in the survival and adaptability of a species under changable environment. Korea is known as original region of cultivated soybean species with high genetic diversity of wild and cultivated soybean lines. We have collected many semi-wild soybean lines, however, the information about the genetic diversity of these lines is limited. And also the phylogenetic relationship and genetic distance among the semi-wild, typical wild types (Glycine soja Sied. & Zucc.), and cultivated soybean(Glycine max (L.) Merr.) have been controversial. This study was carried out to analyze the diversity of the three groups, and to verify their genetic variation and relationships among them. The diversity indexes of typical wild, semi-wild, and cultivated soybean accessions were 0.854, 0.677, and 0.528, respectively. According to the cluster analysis for the genetic relationships, 58 accessions were defined into three genetic groups. Cluster I consisted of 20 cultivated soybean accessions and three semi-wild soybean lines. Cluster II contained 13 semi-wild soybean lines and one typical wild soybean line. Cluster II included 21 typical wild soybean lines and one semi-wild soybean line. Genetic distance between cultivated and semi-wild soybean, typical wild and semi-wild, and cultivated and typical wild were 0.525 0.761 and 0.810, respectively. The relationship between semi-wild soybean was much closer to the cultivated soybean the to the wild soybean
Transgenic plants that over express virus coat protein genes have attracted particular interest from researchers, by virtue of their tolerance to virus infection. The transgenic watermelon rootstock analyzed in this study was established by introducing CGMMV coat protein (cp) under the control of CaMV 35S promoter and NOS terminator (Park et al., (2005) Plant Cell Rep. 24: 350-6). The primary objective of this study was to determine the copy number and integration site of the transgene element, in order to develop detection techniques required for monitoring of the transgenic watermelon rootstock. The Southern blot analysis indicated that a single copy of CGMMV-cp gene was inserted into the genome of transgenic watermelon rootstock. We also identified the genomic sequences flanking the integration site of the transgene by inverse PCR analysis. In an effort to find a sequence usable as an internal positive control for the screening of the watermelon and watermelon rootstock, we found that the Sat and DIP-1 genes appears as one copy within their genomes and is watermelon rootstock- and watermelon-specific. The information of the integrated site and the internal positive control sequence was used to establish a new event-specific PCR-based detection method. In addition, mRNA and protein expression level of the transgene in the transgenic watermelon rootstock and grafted watermelon were investigated. The expression of both mRNA and protein of CGMMV-CP was not detected in the transgenic watermelon rootstocks and watermelons, suggesting that the movement of transgene products from transgenic rootstock to watermelon does not occur at our detection level.
We have discussed here the phenotypic and genetic characteristics as well as proteomic analysis of lesion mimic mutants (LLM) in rice. LLM is one of the mutants that induces cell death without infection of pathogen and produces defense signaling pathways. As the phenotypic expression, most LLMs induce spots on the leaf blades and leaf sheaths at their various developmental stages. We have discussed the nature of bl1, bl2, spl1, spl3, spl4, spl5 and spl6 LLMs in rice which were formed developmentally controlled spot on the leaf blades that were appeared as tiny dotted spots during tillering stage and gradually increased up to maturity. Through Northern blot analysis lower levels of rubisco large subunit and rubisco small subunit were observed in spotted leaves (sp) compared to non-spotted leaves (nsp). However, catalase was severely degraded in the sp. Broken thylakoid membranes of mesophyll chloroplasts were seen in nsp sections and were absent in sp sections of the mutant. Through 2-DE analysis 159 protein spots were differentially expressed between wild type and mutant from identified 800 reproducibleproteins, where 114 spots were up-regulated and 45 were down-regulated. Among quantified 25 protein spots, except two, all of the protein spots including protein disulfide isomerase, transketolase, thioredoxin peroxidase, ATP synthase, and rubisco large and small subunits were identified in the wild type but were absent in the mutant. However, catalase was up-regulated in the mutant. Genetic analysis indicated that studied bl1, bl2 and spl6 mutants are controlled by a single recessive gene.
Crop characteristics of 137 common millet(Panicum miliaceum L.) resources collected in Korea were investigated in order to establish basic data for the improvement of crop breeding. Spike types of 137 plant resources were classified as 4 types of oblate, spreading, oblate-compact(intermediary type between oblate and compact) and oblate-spreading(intermediary type between oblate and compact) type, of which oblate spike type was the highest percentage of 66.4%(91 plant resources) of 137 germplasm. Seed color was classified as 5 colors of dark brown, yellow, brown, yellowish brown, and yellowish white, of which yellowish white was the highest percentage of 66.4%(54 plant resources) among them. Seed type was classified as 2 types of pyramid shape and short pyramid shape, which showed 62.8% and 37.2% respectively. Days from seeding to heading date showed the range from 68 to 85 days with 73.7 days in the average and the highest frequency proportion of it was the group from 70 to 75 days, which occupied 46.7%(64 plant resources) of 137 germplasm. Days from seeding to physiological maturity showed the range from 109 to 124 days with 112.0 days in the average and the highest frequency proportion(60.6%) of it was the group from 111 to 115 days, while there were also occupied with 6.6% below 110 days and 5.1% over 120 days among 137 germplasm. Group from 181cm to 190cm of culm length showed the highest frequency proportion of 47.4%, whereas below 160cm was the lowest occupancy of 2.9%. Spike length showed the range from 181 to 190cm with 181.4cm in the average and the highest frequency distribution of it was the group from 31 to 35cm with the proportion of 54.0%. Number of grains per spike showed the range from 432 to 2,665 grains with 1,429.5 grains in the average and group from 1,200 to 1,400 grains per spike was the highest frequency distribution with the proportion of 20.4%. There were also occupied with 13.1% below 1,000 grains and 8.6% over 2,000 grains per spike among 137 germplasm. Culm length and spike length were positively significant correlation coefficient with days from seeding to heading date. Days from seeding to physiological maturity and percentage of ripened grains were highly significant positive correlation coefficient with 1000 grain weight, showing that the longer and higher the days from seeding to physiological maturity and percentage of ripened grains, the higher 1000 grain weight.
Japonica rice cultivars exhibit high susceptibility to BB disease due to genetic vulnerability in Korea. Korean Japonica rice cultivars mainly posses the genes, Xa1 and Xa3 for BB resistance. These resistance genes are becoming susceptible to K3a, new races of BB, resulting in the breakdown of resistance in high yielding Japonica cultivars. It is imperative to look for novel R-genes for improvement of japonica rice resistant to BB races. This study was carried out to conform useful single gene resistant to 24 BB isolates (including K3a, HB01009) breaking down Xa3 gene. Cultivars and near-isogenic lines (NILs) carrying Xa1, Xa2, xa8, Xa10, Xa11, xa13 genes were susceptible to 24 isolates, whereas IRBB4 carrying Xa4 gene was moderate resistance. IRBB5 and IRBB21 having xa5 and Xa21 genes, respectively, expressed resistance to these isolates. IRBB7 having Xa7 gene showed resistance response to 24 BB isolates, whereas JBB-107 carrying Xa7 gene was susceptible to 10 BB isolates and moderate resistant to 14 BB isolates. Xa7 gene showed different resistance response according to genetic background of used recurrent parent. With these findings, Xa4, xa5, and Xa21 would be the most prospective genes to 24 isolates used in screening.
Crop characteristics of 136 foxtail millet (Setaria italica BEAUVOIS) resources collected in South Korea were investigated in order to establish basic informations for breeding. Spike types of 136 genetic resources were classified as 5 types (conical, clavate, fusiform, cylindrical and top diverge). Among them, conical type was showed the highest percentage(41.2%). Seed of 136 resources was showed yellow color. Spike color of 136 genetic resources were classified as 6 colors (dark brown, light brown, red brown, yellow, yellowish brown and yellowish white). Yellowish white color showed the highest percentage(33.8%) among them. Leaf color of 136 genetic resources were classified as 6 colors (brown, dark green, green, green brown, light green and light brown). Green color was showed the highest percentage(44.9%) among them. Culm length showed a wide variation from 112cm to 183cm (average is 153.8cm) and spike length from 17.7 to 41.2cm (average is 24.1cm). Days from seeding to heading date and days from seeding to physiological maturity were showed the range from 63 to 111 days (average is 90.3 days) and the range from 119 to 149 days (average is 117.7 days) respectively. Number of grains per spike and 1000 grain weight were showed the range from 2,197.0 to 16,980.0 grains (average is 5,839.1 grains) and from 0.6 to 3.1g (average is 1.79g) respectively.
도열병은 전세계적으로 벼에서 피해가 가장 심각한 병중에 하나이며, 특히 이삭도열병의 발생은 수량 및 품질에 치명적인 타격을 입힌다. 벼 이삭도열병 저항성 유전분석을 수행하고자, 이병성인 일미벼를 반복친으로하고, 포장저항성인 화영벼를1회친으로 사용하였으며, 여교잡은 BCnF1세대에 벼이삭도열병 저항성과 연관된 DNA marker RM206을 이용하여 heterozygote인 개체를 이용하였다. BC4F1세대에서 약배양한 DH 집단 110계통을 포장에서 이삭도열병 저항성검정을 수행한 결과 화영벼는 발병되지 않았으며, 일미벼는 58~75%정도 발병되었다. 이 집단의 이삭도열병 발병은 저항성59계통, 감수성 51계통으로 비연속적인 분포를 나타내었으며, t-검정을 수행한 결과 1개의 주동유전자(X2 =0.582, p=0.446)에 의해 지배됨을 확인하였다. 또한, BC3F2 집단을 개체 검정한 결과 저항성 519개체, 이병성 162개체로 우성유전(X2 =0.747 p=0.383)함을 알 수 있었다. 이삭도열병 저항성 연관마커 RM206과 생물검정사이에는 조환가 3.6%로 연관되어 있었다. BC4-DH 집단의 유망계통을 선발하여 생산력 검정을 수행하여 출수기, 간장 등 주요 농업적 특성이 일미벼와 유사한 근동질계통을 선발하였다.
조기재배는4월 하순 ~5월 상순에 이앙하여 8월 중하순에서 9월 상순 사이에 수확하는 재배 작형이며, 특히 추석용 햅쌀은 묵은쌀에 비해 밥맛이 우수하고 판매가 용이하기 때문에 평야지에 조생종 벼를 일찍심어 수확하는 조기재배가 증가되고 있는 추세이다. 그러나, 최근 조생종 품종의 조기재배에 따른 미질저하와 벼줄무늬잎마름병에 대한 피해가 발생하여 문제시 되고 있다. 평야지 조기재배 적응하는 고품질 줄무늬잎마름병 저항성인 품종을 육성하고자 줄무늬잎마름병 저항성이며 밥맛이 우수한 풍미벼와 밥맛이 우수한 고시히까리를 교배한 재조합자식계통(RILs)을 이용하여 농업적 특성 및 품질관련 변이를 조사하였다. 출수기는 준조생으로 모부본에 비해 ±3일 정도 차이로 큰 변이는 없었으며, 대부분의 농업적 형질이 양적인 변이를 나타내었다. 백미완전미율이 높은 상위 10%그룹과 낮은 하위 10%그룹으로 구분하여 농업적 특성 및 이화학적 특성을 비교한 결과 백미완전미율이 높은 그룹은 낮은 그룹에 비해 간장이 크고, 단백질함량이 낮으며, 아밀로스 함량이 높은 경향이였다. 또한 밥의 윤기(식미치)를 측정하여 상위 10%인 그룹과 하위 10%인 그룹을 비교한 결과, 식미치가 높은 그룹은 간장이 크고 백미 완전미율에서 약간의 높은 경향이였으며, 기타 농업적 특성은 큰 차이가 없었다. 간장과 완전미율과는 상관계수 0.41로 유의한 정의 상관이 있으며, 단백질 함량과는 -0.64로 높은 부의 상관을 나타내었다. 따라서, 조기재배의 품질향상과 재배안전성을 높이기 위해서는 초기세대에 단간계통에 대한 강력한 선발이 필요한 것으로 판단되었다.
최근 바이오에너지 사용증가와 국제 곡물가격의 상승으로 수입되는 옥수수 및 수입 조사료의 가격 상승으로 축산농가는 경영비 상승으로 많은 어려움을 겪고 있는 실정에 있다. 따라서 양질 다량의 조사료 확보를 위해서는 배수가 양호한 논을 이용한 사료작물의 재배와 이용에 대한 연구가 필요함에 따라 국산개발 옥수수와 도입 옥수수를 파종하여 논 재배에 알맞은 품종을 선발하고자 이들을 재배하여 논 재배 적응성과 수량성을 알아보았는바 공시재료는 국내 육성 품종인 광평옥 등 2품종과 도입 품종인 P3394 등 4품종 총 6품종을 전라남도 농업기술원에 2007년 4월23일 파종하였다. 시험구 배치는 난괴법 3반복으로 재식거리는 70 x 15cm로 시험구당 12m2로 하고 시비량은 N-P2O5-K2O = 200 : 150 : 150 (kg/ha)로 하였고 기타 비배관리는 농촌진흥청 옥수수 표준재배법에 따랐다. 생육조사는 작물학적특성으로 간장, 착수고, 출사기, 당도, 내습성, 병해, 충해 등을 조사하였고 수량은4열중에 가운데2열을 예취하여 측정하였고 건물수량은 이들중에 3주 샘플을 취하여 건조기에 70℃에서 2주일간 완전히 건조한 후 건물을 측정하여 계산하였다.
남부지역에서 옥수수 유망품종으로는 강다옥과 P3394 등으로 내습성이 비교적 강하고 생육이 양호하며 건물수량은 각각 28.8톤/ha, 25톤/ha 등으로 옥수수도 밭뿐만 아니라 배수한 양호한 논 토양에서 논 재배가 가능한 여름철 사료작물임을 알 수 있었다.
최근 국민경제가 발전하면서 동물성 단백질의 수요증가로 국내 가축의 사육두수가 크게 증가하고 있으며 또한 바이오에너지 사용증가와 국제 곡물가격의 상승으로 수입되는 옥수수 및 수입 조사료의 가격 상승으로 양질의 조사료 확보를 위해서는 논을 이용한 사료작물의 재배와 이용에 대한 연구가 필요한바 타작물에 비해 습해에 비교적 강하고 적응성과 수량성이 우수한 논 재배용 수수류 품종을 선발하고자 본 연구를 수행하였다.
공시재료는 도입 품종인 점보 등 6품종을 전라남도 농업기술원에 2007년 5월3일 파종하였다. 시험구 배치는 난괴법 3반복으로 시험구당12m2 로하고 재식거리는 50cm 간격으로 6열을 파종하였고 시비량은 N-P2O5-K2O= 200 : 150 : 150 (kg/ha)로 하였고 기타 비배관리는 표준 재배법에 따랐다. 생육조사는 작물학적특성으로 초장,출수일,당도,내습성,병해,충해 등을 조사하였고 수량은4열중에 가운데2열을 예취하여 측정하였고 건물수량은 이들중에 3주 샘플을 취하여 건조기에70℃에서 2주일간 완전히 건조한 후 건물율과 사료가치를 분석하였다.
남부지역에서 수수류 유망품종으로는 Revolution, SX17 등으로 내습성이 비교적 강하고 건물수량이 31톤/ha, 25톤/ha 등이며 생육이 양호하며 소화율이 47 ~ 48% 이고 NDF는 각각 64.8%, 70.2% 등이고 ADF는 각각 40.2%, 43.2% 등으로 양호하였다.