콘크리트 구조물은 시공 후 다양한 원인에 의해 물리적, 화학적 변형을 통해 물리적인 성능이 저하된다. 이러한 콘크리트 구조물의 성능저하는 사용수명을 감소하기 때문에 합리적인 보수보강이 필요하다. 최근 콘크리트 구조물의 효율적인 보수을 위해, 부착성능을 향상시킨 하이브리드 보수재료에 대한 연구가 활발히 수행되어오고 있다. 본 연구에서는 기존콘크리트와의 부착성능 및 수밀성을 향상시키기 위해 초속경 시멘트에 PVA 분말수지, 나일론 섬유, 라텍스를 혼입 한 하이브리드 보수재를 개발하였다. 보수재료의 성능평가를 위해 압축강도, 건조수축, 부착강도 실험을 수행하였다. 또한 미리 손상이 발생한 시험체를 제작한 후 보수 전후의 휨부착 성능평가를 수행하였다. 휨강도 평가결과, 기존의 초속경시멘트만 혼입한 시험체를 제외한 모든 실험체에서 110%~150%정도 휨강도가 크게 나타났고, 휨강도에 의해 발생된 균열패턴은 모든 실험체가 기존 콘크리트와 일체 거동하는 것으로 나타났다.

본 연구는 기계주입 방식으로 간단히 재충전 할 수 있는 TPS 공법을 사용하여 실제 건설현장에 적용한 후 콘크리트 균열에 미치는 영향을 알아보고자 하였는데, 그 결과를 요약하면 다음과 같다. 먼저, 초음파 속도의 경우 TPS 공법을 사용하였을 시 주사기 공법에 비해 평균 약 36 mm/sec로 초음파가 빠른 속도로 통과하는 것을 알 수 있었고, 주입깊이의 경우 주사기 공법에서는 부분 충전되는 것을 확인하였으나 TPS 공법의 경우 강한 주입 압력으로 모든 배합에서 보수재가 100% 충전되는 우수한 경향을 나타내었다. 또한, 압축강도의 경우는 TPS 공법 사용 시 평균 16.8 % 증가하는 것으로 나타나 구조적으로 우수한 것으로 나타났다. 이상을 종합하면 주사기 공법에 비해 TPS 공법을 사용하였을 시 균열부위에 주입재가 밀실하게 충전되어 강도 증진으로 인한 품질 향상 및 확인창 설치로 인한 균열보수제의 거동이 확인 가능하다. 또한, TPS 공법 시공구간 532 m에 대하여 약 5일의 공사 기간이 단축됨에 따라 시공성이 우수하여 콘크리트 구조물에 균열 보수공법으로 사용성이 확대될 것으로 기대된다.

Background : The causative organism of Astragalus membranaceus (Hwanggi) root rot disease was known as Pythophthora drechsleri. In this study, a new species of root rot causative organism has been identified and reported. Methods and Results : Hwanggi, which has root rot disease, was collected in Jungsun-gun, Gangwon-do. The diseased root was cut into 1 ㎝ size and disinfected with 1% sodium hypochlorite solution for 1 minute and washed with sterile distilled water. The dried samples were dipped in the PDA medium and cultured in at 25℃ incubator. The 21 isolates were cultured under the same conditions and genomic DNA was extracted. The genomic DNA of isolated strains was extracted using a kit of Biofact. The isolated strains were identified using internal transcribed spacer (ITS) region sequencing. The ITS region was amplied using the primer pair ITS1 (5’-TCCGTAGGTGAACCTGCGG-3’) and ITS4 (5’-TCCTCCGCTTATTGATATGC-3’). The PCR was performed in a final volume of 50 ㎕ containing 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2, each dNTP at a concentration of 0.2 mM, 1.25 IU of Taq polymerase, each primer at a concentration of 10 pmol, and 2ul of the DNA template. Sequencing was performed by asking Macrogen. The analyzed sequences were compared using the Blast program provided by NCBI. As a result of identification of 21 strains, 13 Fusarium spp., 1 Peronospora sp. (causative organism of downy mildew), 3 Acremonium spp., 3 Uncultured fungus, 1 Phyrtophthora sp.. The main pathogen of wilt disease was known as Fusarium sp., a soil-borne pathogen. After analyzing the systematic taxonomic relationships, the systematic diagram was created using the neighbor-joining method. Conclusion : We have finally identified Phytophthora cryptogea as a causative organism of Hwanggi root rot disease and will check whether the disease has occurred on the inoculation through pot test.

Background : Hair care products are mainly prepared by mixing chemicals and natural extracts, such as those obtained from medicinal plants. The purpose of this study was to investigate the effects of 70% ethanolic extracts from the whole plant of Houttuynia cordata and tymosin β4 powder mixtures on the growth of HaCat cells, hair follicle dermal papilla cells (HFDPC), and 3T3-L1 cells. Methods and Results : In the ratio of tymosin β4 more than 10% concentrations, the cell viability of HaCat and HFDPC cell were increased by higher tymosin β4 concentrations. The mixtures of 70% ethanolic extract of H. cordata and tymosin β4 had no toxicity potential to 3T3-L1 cell viability. In this test, the content of thymosin β4 was higher concentration, as the anti-inflammatory effect was increased. The lipid differentiation of 3T3-L1 cells adipogenesis rate significantly increased in a treatment concentration-dependent manner. Conclusion : These results suggest that a optimal mixture ratio of hair growth effect was 70% ethanolic extract of H. cordata solution 50% and tymosin β4 solution 50%. This mixture solution could be used in the development of hair care products.

Background : Lentinula edodes contain a variety of biosurgical substances and a large amount of beta-glucan, which is known for immune-boosting effects. L. edodes was cultivated in nation wide of Korea. And, almost farmhouse using imported strains from Japan and China. Therefore, this study was conducted to prove the excellence of domestic strains to replace imported strains. Methods and Results : In this study, beta-glucan content, which is a unique immune component of domestic and imported strains, was compared to the native immune component of domestic L. edodes strains and imported L. edodes strains. In this test, the Korean strains such as Baekwahyang, Sanjo303ho, Suhyanggo, Cheonbaekgo, and the imported L. edodes strains is Mori290, Moriyujiro. The analysis results are as followings. The proximate compositions were ranged 3.74 - 5.50% in ash, 15.22 - 25.84% in crude protein, 0.93 - 1.43% in crude fat, 6.95 - 9.89% in crude fiber of Korean L. edodes strains and imported L. edodes strains, respectively. Major minerals of Korean L. edodes strains and imported L. edodes strains were potassium (684 - 904.05 ㎎%), calcium (0.46 - 0.65 ㎎%), sodium (8.77 - 11.01 ㎎%), magnesium (30.45 - 33.93 ㎎%). The content of beta-glucan from Korean L. edodes strains and imported L. edodes strains were ranged from 35.69% to 38.68%. Conclusion : As the results of chemical analysis were showed the no difference between Korean L. edodes strains and imported L. edodes strains. These results are meaning that the Korean L. edodes strains quality of the components is not inferior to imported strains. We expected that the Korean L. edodes strains could replace imported L. edodes strains.

Background : Lentinus edodes are highly utilized as food and medicine, and are known to be suitable for preventing diabetes, high blood pressure and arteriosclerosis. L. edodes were known for prevent obesity metabolisms. Also, present consumers persue the healthy and thin condition. Our research perpose is determined the prevent obesity using L. edodes extracts. Methods and Results : The effect on adipocyte differentiation and the accumulation of fat in the L. edodes extract on 3T3-L1 cell preadipocyte and cell toxicity were tested. We measurement to evaluate the inhibition effects of L. edodes extracts on the growth of 3T3-L1 cell adipocytes. The tested cells were treated with L. edodes extracts by ethanolic extracts (concetrations at 10, 50, 100 ㎍/㎖) and hot water extracts (temperature at 50℃, 60℃, 70℃, 80℃, 90℃), respectively. Cell toxicity did not affect the growth of cells growth in hot water extracts. Intracellular lipid accumulation was measured by Oil red O method. The inhibitory effect on 3T3-L1 cell by treatment different ethanolic extract concentrations and temperature of hot-waters. The optimal inhibitory conditions of lipid accumulation by ethanoic extracts and hot water extracts were determined the 32.5% ethanol extracts and 33.3% 50℃ hot water extracts. Conclusion : The results of this study showed that L. edodes extracts had an potentials for reduce lipid accumulation activities. Further more we will examine about the mechanisms of anti obesity activities from L. edodes extracts. This results provides that the basis for antiobesity foods or medicines using Lentinus edodes extracts.

Background : Domestic use of cosmetics has largely been dependent on imports for the opening of cosmetics raw materials, but research has been progressing actively on natural ingredients to develop new materials. Methods and Results : The extracts of hot–water and ethanol extracts from Stachys sieboldii were tested cytotoxicity, inhibition of melanin biosynthesis and NO production activity. The cytotoxicity of the hot water extract of S. sieboldii were 98.4, 95.0, 93.9 and 90.6%, respectively, when treated with 10, 50, 100, 500 ㎍/㎖. Cytotoxicity of S. sieboldii ethanol extract was 97.1, 95.3, 94.6 and 94.4%, respectively, at the concentrations of 10, 50, 100 and 500 ㎍/㎖. Melanin biosynthesis inhibitory effect of α-MSH at 100 μM increased to 118.1% in melanin, but decreased to 92.3% in S. sieboldii ethanol extract. NO production inhibition increased to 109.2% when treated with LPS and was 103.3% at 500 ㎍/㎖ of hot water extract and 105.9% at 500 ㎍/㎖ of ethanol extract of S. sieboldii. Conclusion : The ethanol extract of S. sieboldii did not showed the cytotoxicity, and reduce NO production. Therefore, ethanol extract of S. sieboldii had an potentials for developing whitening cosmetics.

Background : Cornus kousa fruit has been used as a medicine for a long time. Therefore, for the purpose of utilizing C. kousa fruit as a health functional food, antioxidant activity was tested by extracting ethanol concentrations to identify optimal extraction conditions. Methods and Results : C. kousa fruits used in this study were washed, dried and pulverized. Then, the samples and solvents by ethanol concentration (0, 20, 40, 60 80 and 100%) were extracted at 80℃ for 90 minutes at 1 : 20 ratio, respectively. Afterwards, it was filtered, concentrated, frozen and dried. Total phenol and flavonoid content, DPPH and ABTS radical scavenging activity, ferric reducing antioxidant power and oxygen radical absorbance capacity were measured. The total phenol and flavonoid content is the highest in 60% ethanol extract at 20.08 ㎎·GAE/g, 0.86 ㎎·QE/g. In addition to all antioxidant studies showed the highest activitiy in 60% ethanol extract. Also, when HepG2 cells were treated with ethanol extract at 200 ㎍/㎖ concentrations, the survival rate was more than 90% and cytotoxicity was not observed. Then, HepG2 cells were treated with tBHP to induce oxidative stress, and the cytoprotective effect and inhibitory activity of ROS production were measured. As a result, 60% ethanol extract showed the best activity. Conclusion : When extracting C. kousa fruits with ethanol as solvent, It is expected best to use 60% ethanol to maximize potential as a material for health functional foods.

There are very few reports and limited evidence that premedication with steroids decreases early recurrence of ileocolic intussusception after a successful hydrostatic reduction. The purpose of this study was to examine the role of steroids in decreasing early recurrence of idiopathic intussusceptions. A retrospective review of children that underwent successful hydrostatic reduction was conducted. Two groups were identified: group 1 (38 cases) that received intravenous methylprednisolone (1 mg/kg/dose) on diagnosis or immediately after the reduction maneuver, and group 2 (59 cases) were not given. There were 60 boys and 37 girls ranging in age from 6 to 84 months. There were no statistical differences between the groups regarding age, sex, duration of symptoms and laboratory findings on admission. There was no significant difference (p=0.76) in the rate of early recurrent intussusception between the steroid group (15.8%, 6/38) and the non-steroid group (13.6%, 8/59). We found that premedication of children with idiopathic intussusception with methylprednisolone did not decrease early recurrence of idiopathic intussusceptions.

Background : Lentinula edodes (Shiitake mushroom) is a common edible mushroom with a number of potential therapeutic and nutritional applications. However, the growth of Lentinula edodes were classified in accordance with nutrients have no differences in seemingly. The growth characteristics of L. edodes were difficult to find out influenced about between oak and medium. Quantitative real-time polymerase chain reaction (qRT-PCR) is a powerful tool to analyze the mechanisms underlying the biosynthetic pathways of these substances. Methods and Results : A gene encoding amylase AMY was successfully isolated from the L. edodes using RT-PCR. The putative amino acid sequence encoded by AMY showed the highest the homology with the sequence of glycoside hydrolase family 13. We compared the amylase activity and levels of gene expression in L. edodes grown on different breeding materials (oak, and medium), strains from oak (Chunbaegko, and Mori 290), and strains from medium (Tanong, and Carrefour), respectively. Quantitative reverse transcription PCR utilizing pairs of primers specific for AMY gene expression shows that the expression of AMY was induced polysaccharide, and increased during the process of fruiting body formation in L. edodes by medium compositions. Conclusion : This result indicates that amylase may play an important role of growth in morphogenesis of medium condition growth mushroom. The present work will contribute to RT-PCR studies in L. edodes.

The aim of this study was to determine the effect of acute respiratory viral infection on Kawasaki disease. From July 2016 to June 2018, among the patients who underwent respiratory virus screening at the Kwangju Christian Hospital, those diag-nosed 88 patients with Kawasaki disease were enrolled in this study. The effects of acute respiratory viral infection on Ka-wasaki disease were compared according to whether 2nd intravenous immunoglobulin (IVIG) treatment was administered or not, and whether coronary artery complications occurred. Respiratory virus was detected in 44 out of 88 patients. 2nd IVIG treatment rates were 16% (n=7) and 27% (n=12) in the virus positive group and the virus negative group, respectively (p>0.05). The incidence of coronary artery complications was 11% (n=5) and 25% (n=11) in the virus positive group and the virus negative group, respectively (p>0.05). There was no statistically significant correlation between the 2nd IVIG admin-istration and coronary artery complications following the detection of respiratory virus in patients with Kawasaki disease. We think it is necessary to actively study the effects of acute respiratory viral infection on Kawasaki disease through large-scale studies of multiple centers in the future.

The aim of this study was to investigate the factors associated with cerebrospinal fluid (CSF) pleocytosis in pediatric enteroviral meningitis. A retrospective analysis of the medical records was performed in 281 patients under 18 years of age who were diagnosed with enteroviral meningitis by reverse transcription-polymerase chain reaction (RT-PCR) at Kwangju Christian Hospital from January to December 2016. Clinical symptoms and laboratory findings were compared according to the presence or absence of CSF pleocytosis. 112 children (39.9%) did not have CSF pleocytosis. When we compared the group of meningitis without pleocytosis and the group with pleocytosis, age were younger (< 2years), and the interval between onset of symptoms and the time of lumbar puncture was shorter (<24 hours), peripheral white blood cell counts were lower, but C-reactive protein (CRP) was higher in the group of meningitis without pleocytosis. Enteroviral meningitis should not be excluded even if CSF pleocytosis is not seen in patients. And enterovirus CSF RT-PCR should be performed with high suspicion in children younger than 2 years.

최근 다수의 분야에서 딥 러닝을 통한 연구 성과들이 사람의 판단력에 근접하는 결과를 보여주고 있다. 그리고 게임 산업에서는 온라인 커뮤니티, SNS의 활성화가 게임 흥행 여부를 결정할 정도로 중요성이 높아지고 있다. 본 연구는 딥 러닝을 이용해 온라인 커뮤니티, SNS에서 활동할 수 있는 시스템을 구성하고, 온라인 공간에서 사람들이 작성한 텍스트를 읽고 그에 대한 반응을 생성하고 스케쥴에 따라 트위터에 올리는 것을 목표로 한다. 순환 신경망(Recurrent Neural Network)을 이용해 텍스트를 생성하고 글 작성 스케쥴을 생성하는 모델들을 구성했고, 생성한 시각에 맞춰 모델들에 뉴스 제목을 입력해 댓글을 출력 받고 트위터에 작성하는 프로그램을 구현했다. 본 연구 결과는 온라인 게임 커뮤니티 활성화, Q&A 서비스 등에 적용이 가능할 것으로 예상된다.

Background : This study was carried out to introduce Atractylodes macrocephala as a new income element in Gangwon area and to develop the technology necessary for stable quality seedling production. Methods and Results : For the production of high quality seedlings of Atractylodes macrocephala, seedling growth characteristics were investigated according to the types of plug cell size and seedling raising period. Atractylodes macrocephala seeds were sown on February 14, 2018 in 72, 105, 128, 162, and 200 plug trays. The emergence period after sowing was March 2, and the final occurrence rate was 76.6 - 79.5%. The number of days of emergence took 18 to 20 days from sowing date. Growth of seedlings tended to be better with less number of plug trays, such as seedling height, seedling width, leaf length, leaf width and leaf number. On the other hand, roots (net formation) increased rapidly as the number of plug trays increased. After 60 days, the matured seedling rate was good at 75.5 ± 8.4% for the 200 plug tray and 72.5 ± 4.1% for the 162 plug tray. The net formation ratio of matured seedling was the best in 60 days of seeding in 162 plug trays. The rooting rate was 98.0 ± 2.1 - 99.3 ± 1.2% when seeded for 60 days or more regardless of the type of plug tray. Conclusion : In order to produce efficient and stable seedlings in the cultivation of Atractylodes macrocephala in Gangwon area, it was considered to be advantageous for 60 days of seedling settling in the plug trays of more than 162 and less than 200.

Background : Angelica acutiloba is a perennial herb from the family Umbelliferous. The root was used as a substitute for the crude drug. As a by-product of making cheese from whey, it has many proteins and nutritional components, but it has a drawback in that it must be stored in a refrigerator because the storage facility is short. In order to solve such problems, an attempt is made to solve the environmental and economic problems by producing a liquid fertilizer fermented with a large amount of whey. Methods and Results : In this study, we studied the fermentation of wheat germ with lactic acid bacteria (Lactobacillus brevis) and yeast (Saccharomyces cerevisiae). The preliminary experiment was conducted to determine the test concentration of 300 fold dilution and 500 fold dilution. As a result, the application of 300 times dilution liquid prepared by fermentation of whey using lactic acid bacteria showed higher growth and higher growth rate with higher number of leaves and branches. Growth response of the 500 fold dilution was higher than that of the control, but the growth of 300 fold dilution showed the highest. Conclusion : Therefore, it is considered that the 300 fold dilution of Lactobacillus can be used as a substitute for compost when cultivating medicinal plants.

In this study, the development of gravity type collecting device for nuclear dismantling through CFD simulation is shown as follows. As a result of investigating the dust removal efficiency according to the treatment air velocity of 5 m/s (low speed) ～ 30 m/s (high speed), large dusts (80 ～ 100 ㎛) As the wind speed increases, the damping efficiency decreases and the particle size of the dust behavior increases with the increase of the wind speed. As a result, the wind speed (wind speed) 5 m/s is considered to be suitable for improving the vibration damping efficiency.

The purpose of this study was to evaluate the mechanical properties and bond performance of epoxy paint containing graphene materials. Experiment result, it was confirmed that graphene materials are effective to improve the mechanical properties of the epoxy paint, but it is not effective to improve the bond performance.

Recently, the demand for seismic retrofitting has risen rapidly due to the increasing risk of collapse of existing reinforced concrete columns due to the subsequent occurrence of the Gyeon-gju earthquake and the Po-hang earthquake in Korea. In the existing seismic retrofitting method, there are many seismic retrofitting methods such as steel plate reinforcement, fiber reinforcement, and cross-sectional enlargement, but there is a need for an improved method in terms of workability, economic feasibility, durability and maintenance. Therefore, seismic strengthening method of rectangular shaped RC column using rapid fastening of steel bars on the outside of the column is proposed, and the seismic evaluation of the proposed method is investigated through experiments.

The second impact from the edge of cargo was found to be an crucial event causing large volume loss of concrete median barrier. Therefore, in order to improve local impact resistance of concrete median barrier during second impact, local model was developed and evaluations were conducted. Developed concrete median barrier showed the 125 kJ impact level based on conducted analysis.

Recently, overlay concrete median barrier in South Korea was newly developed. Accordingly, numerical analysis for occupant-protection performance was conducted before experimental test. In this study, dynamic analysis of vehicle-overlay concrete median barrier collision was conducted to confirm safety of driver. Furthermore, the analysis results of vehicle behavior was compared to experimental test.