Pemphigus is an autoimmune blistering disease characterized by autoantibodies against epidermal adhesion molecules, desmogleins. Pemphigus vulgaris is most common and shows intraepidermal vesicles caused by the breaking apart of epidermal cells, acantholysis. A 65 years old male patient complained of severe mucosa ulceration on his right mandibular retromolar pad area where traumatic injuries occurred during mastication. He also had multifocal round skin ulcerations, less than 7~8mm in diameter and showed habitual onset and disappeared soon. At this time he was anxious about his oral ulceration with a cancer phobia, thereby a biopsy was made to rule out any malignancy in the ulceration. The histology examination showed multifocal suprabasal splits forming vesicles and erosion. The suprabasal splits were linear and parallel to the basal cell layer. The immunostain of IgK was strongly positive in the vesicular fluid as well as the cell membranes of dissociating keratinocytes, and also positive in many plasma cells infiltrated into the subepithelial zone. TNFα, IL‐1, ‐8, ‐28 for the pro‐inflammatory reaction were weakly expressed, while IL‐6 was strongly positive in the acantholytic keratinocytes of vesicle forming area. β‐defensin‐1, ‐2, ‐3 for the innate immunity were diffusely positive in the involved epithelium. The cell survival proteins, pAKT and HSP‐70 were diffusely positive in the epithelium, while the apoptosis protein, PARP was consistently positive in some acantholytic keratinocytes. These findings indicated that the vesicle formation occurred by autoantibody reaction without the activation of pro‐inflammatory and cell‐mediated immune reactions. The lesion was diagnosed pemphigus vulgaris with abrupt onset of epithelial vesicles at the predisposing areas of traumatic injuries by type II hypersenstitive immune reaction.

The objective of this study is investigation of contamination levels and assessment of health risk effects of heavy metals in herbal pills. 31 Items and 93 samples were obtained for this investigation from major herbal medicine producing areas, herbal markets and on-line supermarkets from Jan to Jun in 2010. Inductively coupled plasma mass spectrometer method was conducted for the quantitative analysis of Pb, Cd and As. In addition, the mercury analyzer system was conducted for that of Hg without sample digestion. The average contents of heavy metals in samples were as follows : 0.87 mg/kg for Pb, 0.08 mg/kg for Cd, 2.87 mg/kg for As and 0.16 mg/kg for Hg, respectively. In addition, the average contents of heavy metals in different parts of plants, including cortex, fructus, herba, radix, seed, algae and others were 0.63 mg/kg, 3.94 mg/kg, 1.42 mg/kg, 1.05 mg/kg, 0.16 mg/kg, 22.31 mg/kg and 10.17 mg/kg, respectively. After the estimations of dietary exposure, the acceptable daily intake (ADI), the average daily dose (ADD), the provisional tolerable weekly intake (PTWI) and the relative hazard of heavy metals were evaluated. As the results, the relative hazards compared to PTWI in samples were below the recommended standard of JECFA as Pb 3.1%, Cd 0.9%, Hg 0.5%. Cancer risks through slope factor (SF) by Ministry of Environment Republic Korea and Environmental Protection Agency was 4.24 × 10−7 for Pb and 3.38 × 10−4 for As (assuming that the total arsenic content was equal to the inorganic arsenic). Based on our results, possible Pb-induced cancer risks in herbal pills according to parts used including cortex, fructus, herba, radix, seed, algae and others were 1.95 × 10−7, 1.45 × 10−6, 2.14 × 10−7, 6.27 × 10−7, 1.99 × 10−8, 3.61 × 10−7 and 9.64 × 10−8, respectively. Possible As-induced cancer risks in herbal pills by parts used including cortex, fructus, herba, radix, seed, algae and others were 1.54 × 10−5, 7.24 × 10−5, 1.23 × 10−4, 2.02 × 10−5, 3.25 × 10−6, 2.18 × 10−3 and 5.67 × 10−6 respectively. Taken together, these results indicate that the majority of samples except for some samples with relative high contents of heavy metals were safe.

Rushton bodies are known to be the aberrant keratinization and calcification in the epithelium of odontogenic cyst, which are similar to the features of calcifying odontogenic cyst and pilomatricoma. However, the pathogenetic mechanism of keratinization and calcification of Rushton bodies has not been clearly elucidated. Here, a case of Rushton bodies found in dentigerous cyst was examined by immunohistochemical method using antisera of PCNA, pAKT, HIF, PIM1, and PARP. The globular keratinization in lamellate fashion showed weak birefringency under polarizing microscope, and the Rushton bodies frequently underwent the dystrophic calcification. The polygonal keratinocytes of Rushton bodies were strongly positive for HIF and PARP, and the cyst epithelium was diffusely positive for pAKT and PIM1. Particularly, the cyst epithelium was hyperplastic and focally invaginated into cyst wall with positive reaction of PCNA. These findings may indicate the active response of odontogenic epithelium against the apoptotic stress of the cyst, producing the globular keratinization and irregular calcification in the polygonal keratinocytes. Therefore, it is presumed that the lamellate keratinization and dystrophic calcification of Rushton bodies are aberrant products of retrograding keratinocytes slowly undergoing apoptotic progresses similar to the phenomena of the ghost cells in calcifying odontogenic cyst and pilomatricoma, and also may have a potential for oncogenic proliferation.

Saliva from the oral cavity was collected from 129 patients with periodontitis and 54 Staphylococcus aureus isolates were isolated from these samples. The S. aureus isolates were tested for their resistance patterns against 15 antibiotics using the disc diffusion method. The percentage of S. aureus isolates resistant to ampicillin was the highest (92.6%). In addition, the percentage of S. aureus isolates resistant to penicillin, oxacillin, cefotetan, cefepime and erythromycin was 90.7%, 11.1%, 11.1%, 9.3% and 5.6%, respectively. However, S. aureus isolates were susceptible to gentamycin, clindamycin, trimethoprim/sulfamethoxazole and vancomycin. 96.3% of S. aureus isolates were resistant to antibiotics. Among them, the percentage of two antibiotic-resistant S. aureus isolates was 74.1%, the percentage of one antibiotic-resistant S. aureus isolates was 5.6%, the percentage of three antibiotic-resistant S. aureus isolates was 3.7% and the percentage of S. aureus isolates resistant to more than 4 antibiotics was 13%. The most common multiple antimicrobial resistance pattern was AM-P. Furthermore, the percentage of methicillin-resistant S. aureus isolates was 11.1% and they were resistant to more than 3 antibiotics.

Resveratrol (RVT) and epigallocatechin gallate (EGCG) individually inhibit adipogenesis in 3T3-L1 adipocytes. The objective was to examine the possibility of interaction between RVT and EGCG, resulting in enhanced inhibition of adipogenesis in 3T3-L1 adipocytes. Preadipocytes were treated with RVT and EGCG individually at 6.25 or 25μM (RVT6.25 or RVT25) and 12.5 or 50μM (EGCG12.5 or EGCG50) and in combination (RVT6.25 + EGCG12.5 and RVT25 + EGCG50). RVT25 as an individual compound decreased lipid accumulation in 3T3-L1 adipocytes by 24%, and RVT25 + EGCG50 further decreased lipid accumulation by 77%. In addition, exposure of 3T3-L1 adipocytes to RVT6.25 + EGCG12.5 and RVT25 + EGCG50 combinations resulted in an enhanced increase of adiponectin release and inhibition of leptin release. Quantitative analysis revealed that the combination of tested materials (RVT6.25 + EGCG12.5 and RVT25 + EGCG50) decreased the expression levels of C/EBPα, PPARγ2, and aP2. These results indicate that the combined treatments with RVT and EGCG produce synergistic effects on inhibiting adipogenesis in 3T3-L1 adipocytes. The overall results suggested that the combining RVT and EGCG might be more capable of exerting antiobesity effects than each individual compound by itself.

종자 건전성(seed health)은 종자 또는 식물체에 유익하지 못한 영향을 미칠 수 있는 생물체의 감염상태를 의미한다. 이들 생물체는 종자의 품질에 직접적인 피해를 줄 수 있으며,감염 종자는 식물체에 병을 유발하고 포장에서의 발병을 확산시킴으로써 대규모의 경제적 피해를 유발할 수 있다. 상업용종자의 해외증식 및 국제교역, 종자 유전자원의 해외분양 및 상호교환이 활발해짐에 따라 이러한 위험성이 더욱 커지고 있으며 더불어 종자 건전성 관리의 중요성 또한 증가하고 있다. 다양한 벼 종자 유전자원을 보존하고 연구용으로 분양하는 국제미작연구소(International Rice Research Institute, IRRI)는 유전자원의 건전성 확보를 위하여 종자건전성연구실(Seed health unit)을 두어 체계적인 관리시스템을 운영하고 있다. 필리핀 식물산업국 산하 식물검역소로부터 권한을 부여받아 필리핀 국내로 도입되거나 해외로 분양되는 유전자원에 대하여종자처리 및 건전성 모니터링, 종자 건전성 검사 및 데이터베이스 관리 업무를 수행하고 있다. 대상 병원균에 따라 육안검사, 수세현탁액 검사, 습지법 검사, 한천배지 검사 등을 적용하고 있으며, 감염종자는 온탕처리와 같은 물리적 처리와 살균제 또는 훈증제와 같은 화학적 처리를 수행한다. 국제미작연구소의 건전성 관리시스템을 우리나라 벼 종자 유전자원의보존·관리에 선별 적용하고, 더불어 종자소독방법과 저장 전관리체계를 확립함으로써 벼 유전자원의 품질을 제고할 수 있을 것이다. 또한 국제협력 및 공동연구 등 신뢰구축을 통해유용한 해외 유전자원을 확보하는 데 기여할 것으로 기대된다.

본 연구는 쌀을 비롯한 저장곡물을 가해하는 어리쌀바구미(Sitophilus zeamais) 성충에 대해서 8종의 식물정유의 훈증독성과 Y-tube를 이용한 후각반응으로 기피효과를 조사하였다. 10㎕/L air의 농도에서 훈증독성시험 결과 pennyroyal과 tea tree 오일에서 각각 97%, 63%의 높은 살충효과를 보였다. 특히, pennyroyal oil(LT50, hour=3.87)은 tea tree oil((LT50, hour=4.09)보다 높고 빠른 살충력을 보였다. 10㎕/filter paper의 농도에서 후각계를 이용한 기피효과는 Cedarwood(80%), Cinnamon(83.3%), Cypress(81.1%), pennyroyal(97.1%) oil이 높은 기피활성을 보였으며, 이보다 낮은 1㎕/filter paper의 농도에서도 Cinnamon(82.4%), Cypress(80.0%), pennyroyal(86.7%) 오일은 높은 기피활성을 나타냈다.

This paper describes the spherical ammonium diuranate gel particles which are the intermediated material of the microsphere for an VHTR(very high temperature reactor) nuclear fuel. The characteristics of the intermediate-ADU gel particles prepared by AWD(ageing, washing, and drying) and FB(fluidized-bed) apparatus were examined and compared in a sol-gel fabrication process. The electrical conductivity of washing filtrate from the FB treating and the surface area of dried-ADU gel particles were higher than those of AWD treating. Also, an internal pore volume in dried-ADU gel particles showed a more decrease in AWD treatment than FB treatment because of decomposition of PVA affected by the washing time. However, the internal microstructures of ADU gel particles were similar regardless of the process variation.

Although the mechanism of the abnormal calcification in the calcifying odontogenic cyst (COC) was not elucidated so far, it has been known that the ghost cells are closely related to the calcification, producing dystrophic globular cementum-like materials, comparable to pilomatricoma in epithelium1). Here, we presented a case of COC occurred in left maxillary canine area of 23 years old female, exhibiting a collection of aberrant ossification admixed with basophilic ghost cells in comparison with seven cases of COC. In the polarizing microscope observation with Masson trichrome stain the present case clearly disclosed the typical birefringence of bony tissue, stained red in von Gieson stain, indicating the collagenous backbone. Some ghost cells showed the features of interdigitating epithelial attachments, empty spaces of nuclei, and reticular basophilic cytoplasms, which were similar to the basophilic ghost cells of philomatricoma. The present case demonstrated the aberrant ossification by basophilic ghost cells in COC similar to the ossification of pilomatricoma

The dental infections have been increased due to the widespread overuse and exposure to antibiotics. One of the well-known pathogens is S. aureus The pathogenic properties of S. aureus is associated with the virulence gene. The objective of this study is to investigate the distribution of virulence gene of S. aureus which may contribute to the prevention and treatment of bacterial infections. 54 strains of S. aureus were separated from saliva taken from 129 outpatients diagnosed with periodontitis from Jun. to Dec., 2010 in Seoul. 44 (46.6%) and 13 (31.7%) strains of them were obtained from 88 and 41 outpatients from the S and E dental clinics, respectively. Then, the distribution of virulence gene and genetic diversity were analyzed with the PCR technique. The result of the S. aureus isolates possessed coagulase gene and showed six polymorphism types 390~470 bp (1.8%), 550~633 bp (3.7%), 630~714 bp (9.3%), 715~795 bp (20.4%), 796~876 bp (40.7%) and 877~957 bp(22.2%) due to variable numbers of tandem repeats present within the gene. In this study it will be anticipated that this study can contribute to establish efficiently the countermeasure about the prevention and treatment of antibiotic bacterial infections.

PURPOSES: Suggestion of asphalt binder constitutive model based on time-temperature superposition principle and overstress concept in order to describe behavior of asphalt binders. METHODS: A series of temperature sweep tests and multiple stress creep and recovery(MSCR) tests are performed to verify the applicability of time-temperature superposition principle(t-Ts) and to develop viscoelastoplastic constitutive equation based on overstress concept. For the tests, temperature sweep tests at various high temperature and various frequency and MSCR test at 58℃, 64℃ 70℃, 76℃, and 82℃ are performed. From the temperature sweep tests, dynamic shear modulus mastercurve and time-temperature shift function are built and the shift function and MSCR at 58℃ are utilized to determine model coefficients of VBO model. RESULTS: It is observed that the time-temperature shift function built at low strain level of 0.1% is applicable not only to 1.0% strain level temperature sweep test but also maximum 500,00% strain level of MSCR test. As well, the modified VBO model shows perfect prediction on MSCR measured strain at the other temperatures. CONCLUSIONS: The Time-temperature superposition principle stands hold from very low strain level to very high strain level and that the modified VBO model can be applicable for various range of strain and temperature region to predict elastic, viscoelastic, and viscoplastic strain of asphalt binders.

본 연구는 쌀을 비롯한 저장 곡물을 가해하는 어리쌀바구미(Sitophilus zeamais) 성충에 대해서 8종의 식물정유의 훈증독성과 후각계(ID 1.5cm, arm 12cm, stem 15cm)를 이용한 기피효과를 조사하였다. 10㎕/L air의 농도에서 훈증독성시험 결과 pennyroyal과 tea tree의 오일에서 각각 97%, 63%의 높은 살충효과를 보였다. 특히, pennyroyal oil(LT50, hour=3.87)은 tea tree oil((LT50, hour=4.09)보다 높고 빠른 살충력을 보였다. 10㎕/filter paper(∅ 5.5cm)의 농도에서 후각계를 이용한 기피효과는 Cedarwood(80%), Cinnmon(83.3%), Cypress(81.1%), pennyroyal(97.1%) oil이 높은 기피활성을 보였으며, 이보다 낮은 1㎕/filter paper(∅ 5.5cm)의 농도에서도 Cinnmon(82.4%), Cypress(80.0%), pennyroyal(86.7%) 오일은 높은 기피활성을 나타냈다.

베트남은 지형학적으로나 기후대가 아주 다양하게 분포함에 따라 생물다양성이 풍부한 국가이다. 생물다양성확보와 유전자원의 안전보존을 위해서 1987년에 베트남 농업 연구청 소속 식물유전자원센터를 설립하여 1996년에 확대 개편하여 운영하고 있다. 베트남 유전자원센터의 정식명칭은 Center of Plant Genetic Resources(PGR)이고, 식물 유전자원의 효율적인 관리를 위해서 지역 연구소를 중심으로 관리기관을 지정하여 식물 다양성 확보, 식물 유전자원 보존 활용을 위한 기술개발 을 촉진하고 있다. 베트남의 국가관리 체계내의 관리기관들은 유용한 유전자원의 수집 관리를 위한 임무를 부여 받았다. 즉 식물자원 센터는 관리체계내의 관리기관의 업무를 조정하고21개 유전자원 관리기관이 맡은 작물을 확실히 관리하여 식물유전자원 유지할 수 있게 할 책임이 있다. 베트남에서 식물 유전자원은 약 300종(species) 25,000점(accessions)이 포장, 기내 및 종자 상태로 보존되어 있는데 매년 500점 이상의 식물자원이 수집되고 1,000점 정도가 분양되어 활용되고 있다. 지난 15년 동안 식물 유전자원센터 소속 관리기관에서 식물 유전자원 보존과 실제적인 이용은 향상되었고, 식물유전자원의 현지 보존도 증가하였으며, 농민과 연계한 현지 보존은 재래종 유전자원의 활용을 촉진시킬 뿐만 아니라 선발 육종으로 이용하고 있다. 그러나 저장시설의 열악성, 포장보존 수집 유전자원의 부적절한 평가, 현지보존의 참여자나 방법론의 부족, 정부지원 예산 및 정책의 부족 등은 유전자원 관리에 중요한 약점이다.

The purpose of this paper is to examine the effects on reliability of equipment or product which spends a great deal of its time in the dormant condition. Many systems experienced periods of dormancy throughout their life cycle, such as periods of operati

Sparganosis is a rare parasitic disease caused by the infection of plerocercoid tapeworm larvae of the genus Spirometra. Up to date many cases of oral sparganosis had been diagnosed from biopsy specimens in Korea, however, in this study a 14 years old male showed an oral sparganosis in his buccal nodule producing its eggs, which were extruded through the ulcerated surface of buccal nodule. In the cytological observation for the pus‐like exudates many ovoid parasite eggs were found with a lot of inflammatory cells, which were mostly composed of eosinophilic polymorphonuclear leukocytes (PMNs). The eggs were measured about 50x50x80 μm, and had an operculum without opercular shoulder. In the subsequent biopsy examination the buccal nodule disclosed a diffuse granulomatous lesion with tunnel‐like spaces containing eosinophilic materials of parasite tegument, also infiltrated with a lot of eosinophilic PMNs. Therefore, we diagnosed it oral sparganosis producing its eggs with the characteristic features of sparganum granuloma in the cytological and histological observations, and also suggested that the cytological finding of parasitic eggs would be helpful to confirm the parasitic infection of sparganum matured enough to produce its eggs.

The aim of this study was to evaluate the status of consumption and consumer satisfaction of dietary fiber supplements. The survey was conducted using a self-recorded questionnaire developed to meet the purpose of this study. The characteristics of the subjects who have taken dietary fiber supplements in the past are as follows: distributions of socioeconomic variables showed that the highest-ranked group(the group that reported the highest consumption of dietary fiber supplements) was professionals with a college degree or higher degree. In addition, the highest-ranked group were the most healthy, nonsmokers and reported drinking habits of ‘1～2 times/week’. Regarding the health status of those surveyed, digestive disease and constipation were the most common complaints. The majority responded with ‘health concerns’ to the question that asked the reason for their purchase of the dietary fiber supplements. Three major places respondents could find dietary fiber supplements were ‘through the internet’, ‘the market’, ‘the pharmacy’ in descending order. The compositions of dietary fiber products varied. ‘konjac’, ‘cellulose’, ‘psyllium husks’ were the most popular components consumed by the subjects. The average duration of product consumption was less than one month. In terms of satisfaction levels(satisfaction was ranked from 1 to 5), ‘taste’ and ‘type’ scored the highest in satisfaction levels while ‘price’ ranked the lowest. When subjects were asked about self-awareness regarding the physiological efficacy of dietary fiber supplements, the responses were positive in ‘bowel movement’ and ‘weight loss’. Further research is required to improve the quality of dietary fiber supplements in order to assist consumers in selecting the appropriate products.

도시화 효과가 여름 강수에 미치는 영향을 분석하기 위해 2010년 8월 13일부터 9월 3일까지 수도권 집중관측(ProbeX-2010)을 수행하였다. 분석 결과, 다음의 두 가지 현상들이 발견되었다. 첫째, 관측 기간 동안 약한 강수(≤1 mm hr-1)가 다른 지역보다 서울 풍하측 지역에서 더 자주 발생하였으며, 이는 최근 5년(2006-2010) 자료에서도 확인되었다. 약한 강수는 주로 서울 풍하측 산악 지역에서 야간에 더 자주 발생하였기 때문에 이는 도시지형 뿐만 아니라 산악 지형의 복합적인 효과로 여겨진다. 둘째, 간헐적으로 대류 시스템이 서울 풍하측에서 급격하게 발달해 호우를 야기했다. 이는 특히 8월 27일 1300-1500 KST의 일련의 레이더 영상에서 뚜렷하게 확인되었다. 본 연구에서는 이 강수 사례에 대한 종관 국지적 날씨 특성과 고층 대기 특성을 자세히 분석하였다. 그 결과 도시지형과 연관된 지표 현열 증가뿐만 아니라 조건부 불안정 대기 상태(특히 대기 하층)와 대기 하층의 습기 유입이 도시화 효과와 연관된 대류성 호우를 야기하는 중요한 요소로 제시되었다.

1. 키르기즈스탄은 중앙아시아의 동쪽에 동서로 길게 위치한 국가로서 국토의 약 90%가 해발 1,500 m 이상의 산악지대, 약 41%는 해발고도 3,000미터 이상의 고산지대, 평균해발은2,750 m이며, 국토면적의 약 4%가 만년설과 빙하로 덮여 있다. 2. 키르기즈스탄은 전통적인 농업국가로서 농업이 경제의 축이며 농촌인구가 65%, 농업부문 종사자가 전 산업에서 차지하는 비율이 36%이고, 농업생산이 국가전체 GDP 에서 차지하는 비중이 20% 이상으로서 비교적 높은 편이다. 3. 이 나라의 농업은 강우량이 적고 건조한 기후조건 때문에 주로 관개에 의존하고 있으며, 관개재배 면적은 약 100만 ha이고, 주요 농작물은 밀, 사료용 호박, 보리, 감자, 옥수수, 사과이다. 특히 큰 일교차와 긴 일조시수를 가진 해발 1,700m의 이시쿨 지역에서 생산되는 사과는 품질이 좋기로 유명하다. 4. 축산업이 농업전체 생산액에서 차지하는 비율이 61%를 넘어설 정도로 중요한데 주로 유목에 의존하고 있으며 유우, 육우,면양이 주된 가축이고 양봉도 중요한 위치를 차지하고 있다. 5. 키르기즈스탄은 맥류, 사과, 양파 등의 원산지일 뿐만 아니라 고산식물종의 다양성이 풍부하고, 특히 사과의 야생종이풍부하게 자생하고 있으며, 비타민 나무 등 많은 약용식물 들도 산야에 자생하고 있다. 6. 키르기즈스탄과는 2005년부터 4년 동안 유전자원 공동수집이 추진되었으나, 정부차원에서 농업기술협력 사업은 거의추진되고 있지 않다. 효율적인 협력 사업을 위해서는 상호간의 국익에 도움이 될 수 있는 과제를 발굴하여 우선순위를 정하고 이에 따라 선택과 집중을 하여야 할 것으로 판단된다.특히 키르기즈스탄은 농업유전자원 저장시설이 없고 관련분야에 대한 관심도 없으므로 금후 키르기즈스탄과는 농업유전자원에 대한 교육과 저장시설의 건설을 통하여 협력관계를 구축하면서 상호 도움이 되는 방안이 적극 모색되어야 할 것으로 사료된다.

식물유전자원에 대한 접근과 이익공유(Access to genetic resources and Benefit Sharing : ABS)에 대한 국제규범은 식량농업식물유전자원국제조약(International Treaty on Plant Genetic Resources for Food and Agriculture : ITPGRFA)과 생물다양성협약(Convention on Biological Diversity : CBD) ABS 의정서(일명 ‘나고야의정서’)가 있는데, 그 목적에 따라 다른체제를 가지고 있다. ITPGRFA는 지속적 농업발전과 식량안보에 기여하는 식량 및 사료작물 64작물에 대해 다자체제를적용한다. 유전자원에 대한 접근과 유전자원을 이용한 후 발생하는 이익에 대해서는 유전자원 제공자와 수령자 사이에 표준물질이전협정(Standard Material Transfer Agreement :SMTA) 체결에 의해 이루어질 수 있다. 상업적 이익이 발생하였을 때는체결한 표준물질이전협정에 따라 수익의 일정 부분을 식량농업식물유전자원국제조약 재정기구에 납부해야한다. 그렇게 지불된 자금은 개발도상국과 경제체제전환국의 농부들에게 직·간접적으로 지원하는데 사용된다. CBD ABS의정서는 ITPGRFA의다자체제와는 다른 자원제공자(혹은 제공국)와 이용자(혹은 이용국)간의 양자체결에 의해서 이루어진다. 생물유전자원 뿐만 아니라 이와 관련된 전통지식을 포함하여 이용을 위해 접근 할경우 사전에 자원제공국이나 제공자에게 허가를 받아야 하며(사전통보승인, Prior Informed Consent, PIC), 유전자원의 이용에서 발생하는 이익을 자원제공자와 이용자간 상호합의조건 (Mutually Agreed Terms, MAT)에 따라 공유하도록 규정한다. 이러한 절차나 내용은 각국의 법으로 규정하도록 되어 있다. 또한 ABS의정서는 PIC나 MAT와 같은 절차의 이행여부를 모니터링 하고, 그와 관련된 내용을 ABS 정보공유소(ABS Clearing House)에 등록하도록 되어있다. 이러한 ABS 국제규범으로 인하여 유전자원에 대한 국가의 주권의식이 높아지고자국의 유전자원의 보호와 관리가 강화됨에 따라 국내 유전자원의 다양성 확보와 보존 및 활용을 위해 국제동향에 맞추어능동적으로 준비하는 것이 필요하다.