Environmental-friendly agriculture(EFA) has been mainly developed at many areas which have factors of natural, social cultural and economic characteristics. These rural areas can be developed into environmental-friendly space to preserve ecosystem, natural and cultural scenery, and environmental resources. The plan for developing a regional agriculture has to get central operating bodies, producers" organizations, a homogeneous district for farming, and so on. This case study is focused on development directions and situations of the EFA of Kosam area in Anseong. Kosam cooperatives is a central managing body to produce and sell rice by duck-farming method, and to develop rural area. This cooperatives is one of the leading those that have developed the regional agriculture in Korea. Kosam area is at a disadvantage of natural -geographical conditions and farming size. Kosam cooperatives has been trying to overcome these agricultural weaknesses by introducing duck-farming method. They have got many excellent results through developing EFA as a regional agriculture. Kosam area will be a good place for green tourism if they can actualize the combination model of three demand factors (natural and agricultural scenic view, natural-friendly recreation and safe food) for green tourism in the near future.

The study must have standpoints for the stable market construction of the Environmental󰠏Friendly agricultural products of the quality󰠏certificated which has rapidly grown due to a discussion of the environment and agriculture, income increase and the interests on foods stability. The survey was conducted through face󰠏to󰠏face interview of 200 adults who are in their twenties or more in Cheonan󰠏city. In this research, the Environmental󰠏Friendly agriculture was a clean agriculture not using a fertilizer or chemicals and the agriculture which protects the environment by preventing the environmental pollution. In the analyses of the consumer"s willingness to pay, the rice showed 69,851 won, and a lettuce and bean󰠏curd showed 947 won and 1,412 won respectively. In terms of current issues of the policy, to establish the stable circulation structure and consumption strategy, there must be a clearness raise of the Quality Authentication (QA) Mark. To raise the trust through quality authentication, there must be transparency raise of information by distribution stages and the thorough post management of the official institutes. Also, to persue the competitive product differentiation, there must be the settlement of the product brand on the market, development of the new production technology and a classification of consumers by incomes. Finally to construct stable distribution and price system, there must be active participation of the local agricultural cooperatives in the distribution of the Environmental󰠏Friendly agricultural products of the quality󰠏certificated and the understanding of the proper price of the consumer market and flexible strategy of the price change.

초다수성 품종 개발의 기초자료로 활용하고자 새로이 도입된 신초형 소얼 수중형 유전자원을 이용하여 F2 -F5상관에 의한 sink-source관련 형질의 유전력을 추정한 결과를 요약하면 아래와 같다. 1. 일품벼에 YR15965 Acp 33이 교배된 F2와 F5세대간 상관정도에 의해 추정된 sink 및 source관련 형질들의 유전력은 specific leaf area, sink-source ratio와 harvest index의 0.009, 0.121, 0.013을 제외한 모든 형질에서 0.224이상의 높은 유전력을 보였다. 2. IR 66738-118-1-2에 가야벼가 교배된 후대에서는 모든 sink 및 source관련 형질이 0.001-0.286의 낮은 유전력을 보였다. 3. Sink 및 source관련 형질들과 수량과는 두 조합 모두 높은 싱관정도를 보였으나 등숙률과는 IR 66738-118-1-2에 가야벼가 교배된 후대에서는 대부분의 형질이 상관이 없었다. 그러나 일품벼에 YR 15965Acp33의 교배 후대에서는 등숙율과 source형질과는 높은 정상관이, sink capacity와 sink-source ratio등 sink형질과는 유의한 부의 상관이 있었다.

의이인과 염주는 동속 근연종으로 외부형태가 유사하고 종피를 깎아 유통하기 때문에 육안으로 식별하기에는 한계가 있다. 특히, 염주는 일부 수입품에서 의이인과 비슷한 크기로 염주 종피를 깎아 유통시키고 있어 진 위 판별에 논란이 되고 있다. 본 연구에서는 의이인의 유통품을 외부 형태적 특징으로만 식별하기 어려운 문제를 해결하고자 내부형태 특성조사와 정색반응, RAPD등을 이용하여 감별을 위한 기초자료로 이용하고자 한다. 1. 의이인의 과피는 석세포로 구성된 층과 대보강세포와 섬유상 보강세포로 이루어진 2층 구조로 구분되는 반면, 염주의 과피는 외과피와 내과피는 석세포로 이루어져 있고 중앙의 중과피는 대보강세포와 섬유상 보강세포로 구성되어 뚜렷한 3층의 구조로 이루어진다. 2. TLC를 이용한 확인시험에서 Hexane : EtoAc = 10 : 1의 조건으로 전개하였을때 중국산 염주에서는 Rf가 0.2에서만 밴드가 형성되었다. 3. 중국의 "상용중약감정대전(常用中藥鑑定大典)"에서 제시된 요오드반응법에 의한 의이인과 염주의 정색반응은 구별이 어려웠다. 4. RAPD 분석에서 8개 primer에서 품종간 다형성 밴드가 관찰되었고, UBC primer 355와 362에서 중국 염주와 의성 염주의 특이 밴드(500bp, 1300bp)가 관찰되었다.

Pseudomonas sp. EL-04J was previously isolated from phenol-acclimated activated sludge. This bacterium was capable of degrading phenol and cometabolizing trichloroethylene (TCE). After precultivation in the mineral salts medium containing phenol as a sole carbon source, Pseudomonas EL-04J degraded 90% of TCE 25μM within 20 hours. Thus, phenol-induced Pseudomonas sp. EL-04J cells can degrade TCE. Following a transient lag period, Pseudomonas sp. EL-04J cells degraded TCE at concentrations of at least 250μM with no apparent retardation in rate, but the transformation capacity of such cells was limited and depended on the cell concentration. The degradation rate of TCE followed the Michaelis-Menten kinetic model. The maximum degradation rate (Vmax) and saturation constant (Km) were 7nmo ℓ/min·㎎ cell protein and 11μM, respectively. Cometabolism of TCE by phenol fed experiment was evaluated in 50·mℓ serum, vial that contained 10 ㎖ of meneral sals medium supplemented with 10μM TCE. TCE degradation was inhibited in the initial period of 1 mM phenol addition, but after that time Pseudomonas sp. EL-04J cells degraded TCE and showed cell growth.

Several microorganisms which degrade phenol and trichloroethylene (TCE) were isolated from the activated sludge of a wastewater treatment plant. Among them, one isolate EL-04J showed the highest degradability and was identified as a Pseudomonas species according to morphological, cultural and biochemical properties. The phenol-induced cells of Pseudomonas EL-04J, which were preincubated in the mineral salts medium containing phenol as a sole carbon source, degraded 90% of 25μM TCE within 20 h. This strain could also utilize some of methylated phenol derivatives (o-cresol, m-cresol and p-cresol) as the sole source of carbon and energy. Cresol-induced cells of Pseudomonas EL-04J also cometabolized TCE.