본 실험은 여름작형 착색단고추의 낮은 플라스틱연동하우스 재배시 유인방법 에 따른 수량 및 품질을 비교하고자 실시하였다. 유인방법은 직립과 경사유인이었고, 과실은 6월부터 11월까지 한달 간격으로 수량, 평균과중, 과육두께, 당도, 경도, 과형, 심실수 등을 조사하였다. 상품수량은 6월에 가장 많았으며, 직립유인이 경사유인보다 더 많았다. 6월의 평균과중은 경사유인이 232g으로 직립유인보다 26g 더 무거웠으나, 7월 이후 직립유인이 경사유인보다 더 무거웠다. 과육두께는 직립유인이 경사유인보다 두꺼웠다. 당도는 온도가 낮아질수록 높아졌으며, 직립유인이 경사유인보다 더 높았다. 직립유인의 과형은 수확시기에 따른 차이를 보이지 않았다. 직립유인의 심실수는 3.27~3.34개로 수확기에 따른 차이를 보이지 않았다.

본 연구는 한우 체세포를 이용하여 생산된 복제란을 한우 대리모에 이식하여 임신이 확인된 개체에서 임신 기간 중 주요 호르몬의 발현 특성을 인공수정으로 임신된 대리모와 비교 분석하고자 실시하였다. 한우 섬유아세포를 이용하여 생산된 체세포 복제란을 자연발정으로 동기화된 한우 대리모에 이식하여 임신이 확인된 개체를 공시하였으며(n=8), 대조군으로는 인공수정으로 임신된 대리모을 사용하였다(n=5). 발정 관찰 후 60일경에 직장검사로 임신을 확인하였다. 주요 스테로이드 호르몬인 progesterone(P4)와 estradiol-l7 (E2) 농도는 방사선동위원소 면역분석시험(RIA) 방법을 이용하였으며, 혈중 cortisol 농도는 ELISA 방법으로 측정하였다. 인공수정한 대리모의 경우 E2 농도가 분만 시기에 급격하게 증가하였으나, P4 농도는 분만 시기에 급격하게 감소하는 경향을 나타내었다. 이에 반해 복제란 이식우의 혈장 P4 농도는 분만 50일 전부터 분만 10일전까지는 대조군과 유사하게 유지되었으나, 분만예정일에는 전혀 떨어지지 않고 높은 수준으로 유지되었다. 한편, 복제란 이식우에서 분만 때까지 정상적으로 임신이 유지된 대리모들의 경우는 임신 기간 동안 cortisol 농도는 임신 후반기까지 낮게 유지되며 별다른 변화를 나타내지 않았다. 반면에 유산이 일어난 개체의 경우에는 임신 100일경에 cortisol의 농도가 급격하게 증가하는 것을 확인하였다. 이상의 결과를 종합하여 보면, 복제란 이식우의 경우 분만예정일 전 후에 일어나는 급격한 호르몬의 변화가 일어나지 않음을 확인할 수 있었으며, 이러한 현상은 복제란 이식우의 분만 지연과 밀접한 관계가 있는 것으로 사료된다.

This study was conducted to analyze the expression pattern of inhibitor of DNA binding proteins (Id)1 and Id2 mRNA on folliculogenesis in rat ovary. The ovaries were obtained from 27 days old Sprague-Dawley rat, fixed, dehydrated, and paraffin embedded. For in situ hybridization, anti-sense and sense Idl and Id2 cRNA probes were prepared and applied to the ovarian section. The ovarian sections were coated with NTB-2 emulsion. After that, the slides were developed and counterstained with hematoxylin and eosin staining. In oocytes, the hybridizational signals of Id1 mRNA were strong in primordial and primary follicles, however, there were no signals in that of atretic or preovulatory follicles. The Id2 mRNA signals were also strong in the oocytes of primordial, primary and secondary follicles. Interestingly, the Id2 mRNA was expressed specifically granulosa cells, but nor in oocyte or theca cells in dominant and preovulatory follicles. Based on these results, Id1 and Id2 mRNA was expressed specifically at follicle stages and follicular tissue and might be closely related with follicle development.

This study was performed to investigate the effects of hCG treatment on pregnancy and delivery rates in the Hanwoo recipients. There were significantly higher pregnancy and delivery rates in the recipients treated with hCG at 7 days after artificial insemination (p<0.05), respectively. The SCNT embryos from bovine fetal fibroblast cells were transferred into the synchronized recipients. The recipients were administered saline (n=89) or hCG (1,500 IU) (n=48) at 7 days after heat, respectively. The pregnancy rate was significantly higher in the recipients treated with hCG compared to that of saline treated group (p<0.01), however, the delivery rate was not different in both treated groups. The concentration of plasma progesterone (P4) was not different in both groups before hCG treatment, but the P4 level was increased significantly in hCG treated group after hCG injection (p<0.05). Although the pregnancy rate was very high in early stage of pregnancy, it was decreased dramatically after 50 days of pregnancy and maintained basal level. Taken together, the treatment of hCG in the SCNT recipients after day 7 of heat was effective method to increase the P4 concentration and to increase the pregnancy rate. But it did not affect directly to delivery.

DNA methylation is one of the reasons for poor survival of clone animals. The OCT-4 gene is essential for maintaining pluripotency of embryonic stem (ES) cells and early embryos. We previously reported that the 5'-promoter region of Oct-4 gene was a target of DNA methylation and the methylation status was changed variously during embryonic development in bovine. The study conducted to examine the expression and methylation pattern of tissue-dependent differentially methylated region (T-DMR) of Oct-4 gene in bovine somatic cell nuclear transfer (SCNT) and in vitro fertilization (IVF) blastocysts. The Oct-4 gene expression was evaluated by RT-PCR and fluorescence immunocytochemistry. The methylation pattern of T-DMR was analyzed using restriction mapping and bisulfite sequencing methods. The Oct-4 transcripts were highly expressed in IVF, while they were not expressed in SCNT. The Oct-4 protein was not detected or expressed at very low level in SCNT, the intensity of Oct-4 protein, however, was strong in IVF. On the other hand, the T-DMR of Oct-4 gene was hypermethylated in SCNT compared to that of IVF. These results suggested that expression and the failure of demethylation of Oct-4 gene was closely associated with incomplete development of SCNT embryos.

Since 1995, the facility of class 1 and 2, classified by law had been subject to system of post maintenance management where periodic safety inspection and diagnosis to identify any problems and to take appropriate measures as a matter of urgency. Although the standards and systems had been revised many times for continuous improvement due to surrounding environmental factors, the technical factors and other factors for improvements, continuous researches and efforts are needed to ensure even higher effectiveness. Furthermore, the consciousness of the management body that executes and implements such improvements for facility management is most important. Therefore in this study, through such analysis, more realistic management consciousness review was performed. Also the direction for improvements is proposed, to carry out more effective facility safety management systems implementation.

일반도로구간에서의 사고발생건수는 2000년부터 2006년까지 감소추세를 보이는 반면 교차로에서의 교통사고건수는 현재까지 꾸준하게 증가하고 있기 때문에 교차로에서의 안전성을 증대시키기 위한 노력이 절실히 필요한 실정이다. 본 연구에서는 신호교차로에서의 도로조건, 교통조건, 교통운영상의 조건 등을 분석하여 교차로의 설계 안전성을 저해시키는 요인들을 찾아내고, 그 요인들과 사고와의 상관관계를 이용하여 지방부 4지 신호교차로의 안전성 판단을 위한 사고 예측모형을 개발하고자한다. 또한 사전에 위험요소를 제거하여 교차로에서의 안전성 평가를 위한 가이드라인을 제시함으로서, 교차로에서의 안전성을 높이는데 그 목적이 있다. 본 연구는 교차로에서의 사고분석을 위하여 비선형 회귀분석을 통해 사고모형을 개발하였고, 이러한 모형들을 이용하여 차대차 사고에 영향을 미치는 주요 설명변수들에 대한 분석을 시도하였다. 모형분석결과, 포아송회귀분석(Poisson Regression)이 모형개발에 가장 적합한 것으로 나타났으며, 사고에 영향을 미치는 변수로는 좌회전 전용차로, 횡단보도, 제한속도, 조명시설, 교차각, ADT 등으로 분석되었다.

This study examined the effects of Web ad components on customer attitude and purchasing decisions in 300 individuals who visited family restaurant Websites. The results are summarized as follows: First, Web ad components such as content, design, and interactivity had effects on the cognitive attitudes of the customers. And design, accessibility, and Web functionality had significant effects on the customers' emotional attitudes. In addition, content, navigation, and Web functionality had significant effects on the customers' behavioral attitudes. Lastly, the cognitive, emotional, and behavioral attitudes of the customers had significant effects on their purchases. From these results, design elements such as content, color, and menus, along with user interactivity for information are considered helpful components of a food service Web ad for customer cognition of the restaurant's information. In addition, aspects of Web functionality such as the food service's Web ad set-up, or the protection of personal information, the color design and menu, as well as accessibility for easy use, are all helpful in terms of gaining a customer attitude that is friendly and preferential towards a restaurant's menu items.

  Most organizations have recently put more emphasis on the improvement in service quality. This phenomenon could come from the recognition that service quality plays an essential role in enhancing organization"s business performance. Hence this paper pre