Epithelial-mesenchymal interaction is well known to have an importance during the organ development as well as cell growth and differentiation. However, in vitro experimental model is not well developed to reproduce in vivo cellular micro-environment which provide a epithelial-mesenchymal interaction. The aims of this study were to develop and evaluate the in vitro experimental model that maintains epithelial-mesenchymal interaction by organotypic raft culture, and to characterize biologic properties of three-dimensionally reconstituted human normal oral kertinocyte(NHOK) and immortalized human oral keratinocytes(IHOK) by histological and immunohistochemical analysis. The results were as follows; 1. Best condition of three dimensionally reconstituted IHOK & HaCaT cells are 14 days air-exposure cultivation, 3 days of submerged state, and dermal equivalent consisting type I collagen and IGF cells. 2. In comparison to IHOK, there was better preservation of the overall epidermal strucutures in oral cracioma cells (HN30) & HaCaT cells by organotypic cultures. But orgnaotypic co-culture of the normal keratinocyte showed the thinnest epithelial layer formation. 3. PCNA was detected primarily in the basal layer of normal mucosa and NHOK, whereas was shown throught the epithellium except surface layer of IHOK cells, and it's expression was similar to that of CIS of biopsied patient's tissue 4. Involucrin is expressed in the upper layer of oral mucosa and NHOK raft, but staining for involucrin was induced in the IHOK rafts indicating differentiation is incomplete, and the staining pattern in the IHOK raft was not uniform. 5. Normal oral keratinocyte raft showed weak immunostaining for p53, and p53 expression of IHOK raft increased rahter than in NHOK. In organotypic cultures of normal cells and IHOK, p53 expression was restricted to the proliferative part of epithelium. This is consistent with expression pattern in biopsy specimens of the normal and CIS tissue. 6. In artifically reconstructed NHOK, the pattern of keratin staining showed both similarity and differences from that of intact normal mucosa. An obvious difference was increased expression of CK10 & CK19, and decreased expression of CK6 in a reconstructed NHOK rather than in normal mucosa, and similar expression was in CK4 and CK16. 7. CK19 & CK16 were strongly positive in HPV immortlaized keratinocyte rafts rahter than in NHOK, an indicator of premalignant or malignant changes, while CK10 & CK6 were decreased, and organotypic cultures of IHOK express was similar keratin expression as epithelial dysplasia or CIS tissue. These results suggest that three-dimensional organotypic co-culture of normal oral & immortalized keratinocytes with dermal equivalent consisting type I collagen and fibroblasts results in similar morphologic and immunohistochemical characteristics to in vivo patient specimens. Thus this organotypic system can be used for studing mechanism of epitehlial-mesenchymal interaction in particular regulating epidermal diffenentiation and morphogenes

This article studied on the community service order in Korea. Community service order is designed to enable the government to save the budget by isolating criminals from the society, and this system protects less serious offenders from learning new skills for the new crimes from old offenders in the correctional office by being held together at one place. Community service order has well established. especially in recent years we attained a remarkable result by this system in the criminal policy. in practice, however, there are some problem to improve the use community order service system more efficiently, the most critical problem is about probation officers. to polish and enforce this system, we should train and develop the professional manpower, such as probation officers. The next problem is probation officer needs to develop community service order programs to give criminals more chance to participate in these programs positively. Finaly, our a warm affection for the criminals will lead community service order to the success.

본 연구에서 소 미성숙 난자의 성숙 배양 시 세포주기 억제제인 cycloheximide, 6-DMAP, rosco-vitine를 첨가하였을 때 난자의 감수분열이 억제제비 첨가난자에 비해 높은 비율로 germinal vesicle 기에서 정지된 것이 관찰되었다. 또한 cyclohexi-mide, 6-DMAP, roscovitine을 배지에서 제거한 후 다시 추가적인 체외 성숙 배양을 실시하여 ger-minal vesicle기에 정지된 세포주기의 진행이 재

종빈돈의 번식효율 증진을 위해 후보종빈돈의 분만후 포유기간, 등지방두께 등이 번식능력에 영향을 미치는 요인을 조사ㆍ분석하여 우수한 후보 종빈돈의 조기선발에 활용코자 수행한 결과는 다음과 같다. 1. 분만모돈의 포유기간이 17∼21일일 때에 등지방두께는 19.19mm였고, 포유기간이 22∼26일 일때에는 16.52mm로 포유기간이 길수록 분만모돈의 이유시 등지방두께는 유의적으로 얇았다.(p<0.01). 2. 분만모돈의 포유기간이 17∼21일인 경우에 발정

종빈돈의 번식효율 증진을 위해 후보종빈돈의 성성숙일령, 첫교배일령 및 등지방 두께 등이 번식능력에 영향을 미치는 요인을 조사분석하여 우수한 후보종빈돈의 조기선발에 활용코자 수행한 결과는 다음과 같다. 1. 출생계절별 첫발정일령 및 첫수정일령은 봄에 출생한 후보종빈돈은 194.14일 및 222.05일, 여름에 출생한 후보종빈돈은 163.25일 및 193.00일, 가을에 출생한 후보종빈돈은 160.25일 및 199.20일, 겨울에 출생한 후보종빈돈은 159

본 연구는 백운산의 식물상을 조사하기 위해 수행되었으며. 기간은 2000년 10월부터 2001년 8월까지 조사하였다. 충북 백운산(1,087m)의 관속식물상을 조사한 결과, 90과 273속 411종 1아종 49변종 7품종 등 총 468종류(Taxa)가 확인되었다. 그리고 본 대상지의 희귀 및 멸종위기식물을 조사한 결과 천마. 말나리, 금강제비꽃, 매미꽃. 도깨비부채, 쥐방울덩굴, 개족도리, 태백제비꽃, 미치광이풀. 토현삼 등 총 10종류로 나타났으며. 한국 특산식물은 개족도리. 가는장구채, 할미밀망, 진범, 매미꽃, 흰털괭이눈, 조팝나무, 가시딸기, 광릉갈퀴, 뽕잎피나무, 서울제비꽃, 금강제비꽃, 자란초, 토현삼, 병꽃나무, 털잔대, 고려엉겅퀴, 각시서덜취, 분취 등 19종류로 조사되었다.

본 연구에서는 국내?외에서 유통되는 건강관련식품 중 성기능 개선 및 효과에 대하여 광고?판매하고 있는 제품들에 대하여 최근 의약품으로 허가된 발기부전치료제인 sildenafil, vardenafil, tadalafil 및 sildenafil의 유사물질인 homosildenafil을 대상으로 정성과 정량을 위한 동시분석법을 개발하고 유통 제품 중의 함량을 분석하여 함유 실태를 파악하고자 하였다. 네 가지 성분을 확인할 수 있는 TLC와 LC/MS 분석방법을 개발하였으며, LC/PDA를 사용한 동시 정량분석법을 개발하고, 건강보조식품 등 건강관련 식품 중 sildenafil 등 4종 물질에 대해 총 35개의 시료를 분석한 결과 sildenafil이 7종에서 0.4mg/g~360.9mg/g, homosildenafil이 7종에서 2.2gm/g~336.0mg/g, tadalafid이 2종에서 각각 19.2mg/g, 429.3mg/g로 검출되었으며, 본 연구에서 개발한 동시분석법은 유통식품에 적용 가능하였다.

실험장비의 용량 제약, 경제적인 이유 등으로 지진하중에 대한 구조물의 동적 거동을 연구할 경우, 보편적으로 축소모형이 많이 적용되고 있다. 그러나 구조물의 지진응답은 비탄성 거동을 나타내기 때문에 거동예측이 복잡함에도 불구하고, 축소모형의 지진실험 결과로부터 원형구조물의 지진응답을 유추하기 위한 상사법칙의 연구는 미비한 실정이다. RC구조물의 축소모형 제작 시 상사율이 커지면 상대적으로 부가질량이 증가하며, 또한 굵은 골재 크기의 영향으로 원형구조물과 축소모형의 제작에 동일한 재료를 사용하지 않는 것이 바람직하다. 따라서 동일한 재료를 사용하지 않을 경우, 상사법칙은 기하학적인 상사율과 재료적인 등가탄성계수비에 의존하게 된다. 본 연구에서는 원형구조물과 축소모형에 각각 적용되는 normal-concrete와 micro-concrete의 재료 비선형성을 파악하기 위해 압축강도시험을 수행하여, 재료의 거동구간을 등가의 다단계로 나누어 등가탄성계수비를 적용시킴으로써 지진손상의 정도를 고려할 수 있는 Equivalent multi-phase similitude law를 유도하였다. 이러한 상사법칙을 적용한 유사동적실험 알고리즘을 구축하여 수치해석적인 검증을 수행하여 유사동적실험의 적용성을 확인하였다.

FIMS (Far-ultraviolet IMaging Spectrograph) is the main payload of STSAT-1 satellite which was successfully launched on September 27, 2003. The optical system of FIMS consists of two sets of parabolic cylinder mirror, slit, ellipsoidal reflection grating, and baffle system. We designed two types of baffle system for the FIMS: FOV baffle and order baffle. FOV baffle in the mirror house controls the field of view, and the order baffle in the vacuum box blocks the rays reflected rays by different orders.

The characteristics of the BOES (Bohyunsan Observatory Echelle Spectrograph) CCD camera is presented. In order to get optimum gain and readout noise of the CCD, we examine the variation of the gain and readout noise by changing the value of output drain voltage of the CCD and measuring the gain using transfer curve, which is defined as the plot of variance versus mean exposure level of a homogeneous light onto the CCD surface. The gain and readout noises are optimised to be 0.5e −/ADU and 3e−, which is good for highest signal-to-noise ratio and contrast for the low light level characteristics of the BOES. We also measure the dark count of the CCD by getting five dark images with 3600 seconds exposure time. The mean dark count from median stacked dark images is essentially zero. A table of positions of defected pixels is also presented.

A CCD camera for the BOES (Bohyunsan Observatory Echelle Spectrograph) has been developed. The camera consists of a 2048 × 4096 format CCD, a SDSU Gen-I CCD controller, and a continuous flow cryostat (CFC) designed by the ESO. In order to control the CCD under SDSU Gen-I controller, the voltage level of all the biases and clocks were lowered by -6V. The CFC showed cooling time of about 10 hour, after which the chip temperature settled down with variation less than ±1°C. The final chip temperature is around -105 °C with the setting value for the CFC as -170 °C

Considering the established research about the characteristics of traditional expression in a contemporary korean architecture, there are two critical problems. the one is that the extent of a case is limited to a public building and a large exhibition building. the other is that the angle of research focus on the viewpoint of specific a level. To settle these problems and supplement a established research, this study is to investigate and analyze the characteristics of popular traditional expression in small commercial buildings. In A viewpoint on the expansion of a case and a various angle, A small commercial buildings is selected as a case. because it is felt that the populace's taste vis-a-vis traditional expression. To investigate and analyze, A main analytic criterion is follows. 1) A expressional method on traditional architectural form 2) A expressional aspect by architectural element 3) A expressional aspect in architectural material 4) A expressional aspect in architectural structure. In consequence we can find out the following results in the process of analysis. 1) In expressional method on traditional architectural form, the traditional expression is represented as the imitation and transformation of a traditional folk house forms, and the embodiment of traditional image 2) In expressional aspect by architectural element, the traditional expression elements is mainly represented at a straw roof and a mud-plastered wall 3) the traditional expression material is mainly straw, timber, small wood boards and mud 4) In expressional aspect in architectural structure, the traditional expression is represented from the viewpoint of decorative expression.

In 2002, a new solar spectroscopic system with the Coelostat type has been installed at Korea Astronomical Observatory. It was designed to observe solar spectra in the range from 3000 to 8000 Å with the spectral resolution of 1 Å/mm. The system is composed of a 40cm diameter Coelostat with 9m focal length, spectroscopic system with 600groove/mm grating, and a IK × IK CCD detector. By developing observational softwares for this system, we have successfully observed solar Ha spectra. In this paper, we development of telescope control and observational softwares.