Since 1982, female agricultural workers are populous than male in Korea. Previous study was suggested that female workers are more sensitive to the agricultural medicine poisoning than male. This study was conducted with the aim of evaluating the impact on plasma components and nutrient intakes in female agricultural workers by the use of pesticides. Data were obtained from 44 females residing in Wonjoo, Kangwon-do area. Analysis for the general characteristics, nutrient intakes, and plasma components of the subjects were performed by physical examination, 24-hour recall method and venous blood sampling. They were divided into 3 groups according to the degree of the pesticide use: 8 in none(Non-expose group), 14 in low(〈4/yr)(Low-expose group), and 22 in high(≥4/yr)(High-expose group). The results are summarized as follows. Serum glucose, SGOT, SGPT, triglyceride, HDL-cholesterol did not correlated with pesticide use and were not different among the groups. But serum total cholesterol was higher in pesticide use group. With increasing the number of pesticide use times, leukocyte count was tended to increased. Eighty two percent of the subjects were hemoglobin less than or equal to 12g/dl. Hematocrit, MCV, MCH, MCHC, serum Fe, Serum ferritin belonged to normal range and did not correlated with pesticide use. Intake of energy, Fe, vitamin B2 were lower than RDA. Fe, K, and vitamin A intake were higher in don't use group than the other groups. There are not many differences according to using the pesticide in anthropometric measurements, nutrient intakes, and blood components, except for serum total cholesterol level and some nutrients intake. But these results suggest the need the systemic researches about the effects the pesticide using on nutritional status in Korean agricultural workers.

Acetylcholinesterase(AChE) is an enzyme which hydrolyses acetylcholine into choline and acetate. Glucose and Adenosine-5'-Triphosphate(ATP) are the primary energy source for the brain and therefore defects in glucose metabolism and the mitochondrial system can affect cognitive function. The fact that the level of acetylcholine(ACh), neurotransmitter, glucose and ATP, are found to be decreased in the brain of Alzheimer patients has lead us to focus on the inhibition of AChE for the developmental research of the Alzheimer's disease(AD) treatment. In this paper we describe the effects of herbal medicine 9908(滌痰化瘀湯加減) in vitro and in vivo. As herb 9908 concentraion increased, the Vmax values of AChE from Electric eel were decreased, which showed that the activity of AChE in vitro was decreased. Also herb 9908 inhibit AChE activity in vivo. When herb 9908 was orally administrated to rats, amount of glucose and ATP which are energy source in metabolism, increase in brain and blood of rats. These result are also interesting in their effects for AD treatment compare with comercial drugs such as Cognex and Aricept. Furthermore, when considering the toxic problems of the Cognex and the Aricept, these oriental medicines could be very valuable medicine for treatment of AD. Abbreviation AD: Alzhemer's disease, AChE: acetylcholinesterase, ACh: acetylcholine, ATCh: acetylthiocholine, DTNB: 5,5‘-dithio-bis-2-nitrobenzoate, ATP: Adenosine-5'-triphosphate GAPD: Glyceraldehydephosphate dehydrogenase PGK : phosphoglycerate phosphokinase

양극산화법을 이용하여 광촉매 특성을 나타내는 TiO2 피막을 제조하고 염료의 분해반응을 통하여 광분해 효율을 조사하였다 양극산화법에 의해 제조된 산화피막은 모두 광촉매 분해특성이 있었으며 양극산화의 조건에 따라서 TiO2 피막의 성장거동과 피막 형태에 차이가 나타났다. 황산용액에서 양극산화된 TiO2 피막은 불규칙적 인 입자모양으로 anatase와 rutile이 혼합된 조직이었으며, 인산이 첨가된 혼합용액에서 형성된 TiO2는 anatase로 셀 모양의 피막형태로 생성되었다. 광촉매 특성에 적합한 양극산화의 인가 전압은 180V인 것으로 나타났다.

고순도알루미늄 유전체의 내부표면적을 증가시키기 위하여 1M의 염산 에칭용액에 첨가제를 사용했을 때 나타나는 에칭특성의 변화를 조사하였다. 염산용액에 에틸렌글리콜이 첨가된 혼합용액에서 에칭을 실시했을 경우 알루미늄 기지 표면에 미세하고 균일한 에치피트가 형성되어 표면적 증가 효과가 크게 나타났으며, 또한 양극 산화 후 측정된 정전용량의 결과에서도 에틸렌 글리콜이 첨가된 에칭액에서 제조된 유전체는 표면적 증가에 의한 높은 정전용량 값을 나타냈다.

Paralytic shellfish poison (PSP) occurrence and variation in the different bivalve species including oyster, Crassostrea gigas and mussel, Mytilus edulis in Jinhae bay Korea from January to December in 1997 were surveyed. And also compositional characteristics of PSP ingredients in the different bivalve species were investigated. PSP in shellfish was detected from late February and increased continuously until the middle of April in Jinhae bay. And after April PSP level had been decreased gradually and the toxicity was not detected by mouse bioassay in the early of June. Of the examined bivalve species, PSP content in the mussel exhibited the highest value and the PSP content in the mussel in the middle of April, PSP high season in Jinhae bay, was 6 times higher than that in the oyster. Gonyautoxin (GTX)1-4 group occupied 59.0-78.8% of whole PSP contents and identified as dominant ingredient in the examined bivalves except oyster. And it is also identified that the PSP toxicity in the tested species were derived from the GTX group. And the dominant ingredient of PSP in the oyster was carbamoyl-N-sulfo-llα- hydroxysaxitoxin sulfate(C1)(37.9%) and neosaxitoxin(neoSTX)(26.2%). But the toxicity of C1 in the tested oyster could be ignorable and most toxicity (80.0%) was derived from saxitoxin (STX) group.

This study was undertaken to access the effects of collection method, room temperature at oocyte recovery and culture media on the oocyte quality, fertilization and cleavage rates of in vitro matured and fertilized oocytes of Korean native goats. Ovaries obtained from a slaughterhouse were transported to the laboratory and were divided into 2 groups. One group of ovaries was maintained at 30 to 35 of the room temperature and another group was remained at 20 to during oocyte recovery. The oocytes were recovered by follicle aspiration, slicing and aspiration+slicing methods from 3 groups of follicles according to size; <2 mm, 2 to 6 mm and >6 mm. The matured oocytes were inseminated with buck epididymal spermatozoa at a concentration of 3~3.510 m1 and fertilization was identified when 2 pronuclei were present in the cytoplasm. Although the recovery rate per ovary obtained by the combination of follicle aspiration + slicing(19.62.2) method was higher than aspiration(11.71.1) and slicing(14.81.8) collection, optimal recovery according to oocyte grades resulted form ovarian slicing compared to aspiration or combined methods(P<0.05). However, no significant differences were found in the mean number(2.51.8; 3.33.3; 2.92.4) and the proportion of favorable oocytes(Grades I, II and III) recovered(31.6%, 36.0%, 36.4%,) according to follicle size(<2 mm; 2 to 6 mm; >6 mm). Fertilization rate was 60.0%, 67.7%, 70.6% and 56.4% and the proportion of embryos/zygotes was 11.1%, 7.1%, 5.0% and 2.8% in 20~/BO, 30~35/BO, 20~/TALP and 30~35 /groups, respectively.

This study was conducted to develop an improved method for oocyte pick-up(OPU) with finger-sensibility using ultrasound-guidance from ovarian follicles in Holstein heifers. Oocytes were aspirated from ovarian follicles of clear-outline (>2mm), obscure-outline and invisible( 2mm) on ultrasound images with 3 different vacuum pressure(40, 80, 120mmHg). Total number of oocytes recovered/follicles were 309/237(130.4%). 113/80(141.3%) and 107/74(144.6%) with 40, 80 and 120 mmHg of vacuum pressure, respectively. Mean number of oocytes recovered was higher in 2 OPU/week (18.35.3) than 1 OPU/week(14.54.1), but this difference was not statistical1y significant. The recovery rates were not affected by the number of OPU as 135.6%(282 oocytes/208 follicles) in 1~20 OPU, 137.7% (168/122) in 21~40 OPU and 148.4%(92/62) in 41~60 OPU, respectively. The proportions of good oocytes (Grades I) recovered were not significantly different by the number of OPU until 40 OPU(12.4% in 1~20 OPU vs 16.7% in 21~40 OPU). However, a significantly(P<0.05) lower recovery rate resulted from more than 40 OPU compared to less than 40 OPU(7.6%). These results imply that more fertilizable oocytes can be produced from invisible-immature follicles by transvaginal aspiration with finger-sensibility from Holstein heifers.

본 연구는 해외근무자들의 이문화 적응, 역할외 행동 및 조기귀환의도와 관계가 있는 개인적 특성들을 살펴 보았다. 특정 기업집단에서 파견한 관리자들을 대상으로 하여, Global Leadership Institute에서 개발한 해외근무자들의 개인적 특성 개념 및 측정항목들을 중심으로 데이터를 수집하고, 분석하였다. 그 결과는 다음과 같이 요약해 볼 수 있다. 해외근무자들의 이문화 적응은 그 구성요소에 따라 관련 개인적 특성들이 차이가 나타났다. 예컨데 일반적 적응의 경우, 문화적 유연성이 가장 유의한 관계를 보였고, 사회적 적응의 경우는 사교성과 학습지향성이 한계적이나마 관계가 있는 것으로 나타났으며, 직무적응의 경우에는 다면적 리더십과 학습지향성이 통계적으로 유의한 관계를 보였다. 한편 해외근무자의 역할외 행동은 영향력 주체에 대한 신념이 가장 유의한 개인적 특성으로 나타났고, 조기귀환의도는 협동적 갈등해소 스타일, 영향력 주체에 대한 신념이 통계적으로 유의한 관계를 갖는 것으로 나타났고, 다면적 리더십과 학습지향성은 한계적이나마 관계가 있는 것으로 나타났다. 끝으로 이러한 연구결과가 갖고 있는 한계, 시사점 및 향후 연구과제들에 대한 논의를 하였다.

It is realized that the extraterrestrial matter is in ionized state, plasma, so the matter of this kind behaves as not expected because of its sensitiveness to electric and magnetic fields and its ability to carry electric currents. This kind of subtle change can be observed by an instrument for the magnetic field measurement, the magnetometer usually mounted on the rocket and the satellite, and based on the ground observatory. The magnetometer is a useful instrument for the spacecraft attitude control and the Earth's magnetic field measurements for the scientific purpose. In this paper, we present the preliminary design and the test results of the two onboard magnetometers of KARl's (Korea Aerospace Research Institute) sounding rocket, KSR­III, which will be launched during the period of 2001-02. The KSR-III magnetometers consist of the fluxgate magnetometer, MAG/AIM (Attitude Information Magnetometer) for acquiring the rocket flight attitude information, and of the search-coil magnetometer, MAG/SIM (Scientific Investigation Magnetometer) for the observation of the Earth's magnetic field fluctuations. With the MAG/AIM, the 3-axis attitude information can be acquired by the comparison of the resulting dc magnetic vector fields with the IGRF (International Geomagnetic Reference Field). The Earth's magnetic field fluctuations ranging from 10 to 1,000 Hz can also be observed with the MAG/SIM measurement.

2000년 4월부터 10월까지 총 3회에 걸쳐 통영 연안해역 10개 정점 표층수와 저층수에서 종속영양세균, 대장균군세균, 균류의 계절별 분포를 조사하였다. 조사기간 중 미생물의 생균수는 평판계수법으로 측정되었고 종속 영양세균은 표층수에서 4월 3.1×102- 4.0×103cfu ml-1, 8월 2.7×103- 1.2×10-5 ml-1, 10월 1.3×

질산염과 인산염의 농도가 다른 배지에서 Gymnodinium sanguineum과 Sketetonema costotum의 성장과 세포구성성분을 비교 분석하였다. 질산염은 0, 0.3, 0.6, 0.9, 1.2 mM, 인산염은 0, 15, 30, 45, 60μM을 각각 첨가한 배지에서 배양하였을 때, 저농도의 질산염 및 인산염 배지에서 G. sanguineum의 성장은 억제되었으나, S. costatum은 성장에 영향을 받지 않은 것으로 나타났다.