Recently, in Korea various kinds of genetically modified (GM) crops have been imported and used as a raw material to manufacture foods and livestock feeds, but the different social concerns about the benefits and the potential risks of GM crops are being shown with a different reaction from the public. Thus a persistent management is required for the safe utilization of genetically modified organism (GMO). PCR analysis of transgene into crop is generally performed for the efficient post management of GMOs. The most important prerequisite for the application of nucleic acid detections is to decide the effective DNA-extraction methods. Particularly, in the case of processed feeds, the nucleic acids of which may be damaged by heating, high pressure, pH treatments, fermentation, etc. in processing, DNA must be extracted with high sensitivity from the samples to perform the PCR successfully. In this study, seven of DNA-extraction methods used commercially and non-commercially were compared with respect to the yields and quality of DNA extracted from livestock feeds and those crop materials. Amounts of genomic DNA obtained from the extraction methods varies according to feed configurations and crop materials. The DNA yield and uniformity of samples extracted with PG, CTAB, and QF method is greater than that obtained from other extraction methods. In the DNA integrity of the selected extraction methods, PCR analysis showed distinct amplifications and similar patterns in detecting crop endogenous genes and GMO genes. These results would be applicable for the selection of an adequate DNA-extraction method in extracting processed feeds and/or crop materials.

식품에서 대장균군을 효율적으로 분리하기 위해 대장균군 선택배지 5종: Chromocult Chromocult coliform agar (Merck), Hicrome coliform agar (Sigma), CHROMagar ECC, Brilliance E.coli/coliform medium (OXOID), endo agar (Merck)을 선별하여 식품 분리 대장균군 83종 및 표준균주 21종에 대해서 민감도와 특이도를 분석한 결과 Chromocult coliform agar와 HICrome coliform agar에서 94%도 민감도가 가장 높았으며, Brilliance E.coli/coliform medium는 93%, CHROMagar ECC는 92%, ENDO agar는 74%의 민감도를 나타냈다. Chromocult coliform agar와 HICrome coliform agar는 대장균군의 회수율도 높았다. 그러므로 Chromocult coliform agar와 HICrome coliform agar는 대장균군을 분리하는데 가장 효율적인 배지로 생각된다.

This paper considers the allocation and engagement scheduling problem of interceptor missiles, and the problem was formulated by using MIP (mixed integer programming) in the previous research. The objective of the model is the maximization of total intercept altitude instead of the more conventional objective such as the minimization of surviving target value. The concept of the time window was used to model the engagement situation and a continuous time is assumed for flying times of the both missiles. The MIP formulation of the problem is very complex due to the complexity of the real problem itself. Hence, the finding of an efficient optimal solution procedure seems to be difficult. In this paper, an efficient genetic algorithm is developed by improving a general genetic algorithm. The improvement is achieved by carefully analyzing the structure of the formulation. Specifically, the new algorithm includes an enhanced repair process and a crossover operation which utilizes the idea of the PSO (particle swarm optimization). Then, the algorithm is throughly tested on 50 randomly generated engagement scenarios, and its performance is compared with that of a commercial package and a more general genetic algorithm, respectively. The results indicate that the new algorithm consistently performs better than a general genetic algorithm. Also, the new algorithm generates much better results than those by the commercial package on several test cases when the execution time of the commercial package is limited to 8,000 seconds, which is about two hours and 13 minutes. Moreover, it obtains a solution within 0.13 ~33.34 seconds depending on the size of scenarios.

Recently, nanotechnology has grown as one of the leading science technology along with other converging technologies such as biology, information, medicine etc., bringing the continuous investment of the government in nano-related field. However, it is difficult to measure and evaluate the performance of the national research and development programs because of the multidimensional character of the expected outcomes. This study aims to measuring efficiency of the national nanotechnology research and development programs using DEA model. The decision making units are nine nano-related ministries including the Ministry of Science, ICT and Future Planning. The input variables are total expenditure, number of the programs and average expenditure per program. The output variables are science, technology and economic indicator, and the combination of these outputs are respectively measured as seven different DEA cases. The Ministry of Science, ICT and Future was the first efficient ministry in total technical efficiency. Ministry of Agriculture, Food and Rural Affairs and the Ministry of Food and Drug Safety were efficient in pure technical efficiency, when the Ministry of Commerce Industry and Energy took the first in the scale efficiency. The program efficiency was affected by organizational characteristics such as the institution’s scale, the concentration of the research paper or the patent, technology transfer or the commercialization. The result of this study could be utilized in development of the policy in the nanotechnology and the related field. Furthermore, it could be applied for the modification of expenditure management or the adjustment of the research and development programs’ input and output scale for each ministry.

This study applied the Stochastic Frontier Analysis to estimate which independent variable affects to efficiency of aquaculture household. This study used wage and facility scale as input variables, sales volume as an output variable to estimate efficiency. Also, the study used region, species, water quality to estimate technical inefficiency factors of the model. The data used for this study were obtained by the operating costs survey using 1:1 interview method. The study selected translog production model with technical inefficiency term estimated as half-normal distribution. In addition, the study used pearson and spearman correlation coefficient among efficiency estimating models. Also, the study analysed differences among estimated efficiencies through t-test, and showed us 0.1793 in species, 0.4677 between Geojae and Masan.

PURPOSES: The demand for extending national highways is increasing, but traffic monitoring is hindered because of resource limitations. Hence, this study classified highway segments into 5 types to improve the efficiency of short-term traffic count planning. METHODS : The traffic volume trends of 880 highway segments were classified through R-squared and linear regression analyses; the steadiness of traffic volume trends was evaluated through coefficient of variance (COV), and the normality of the data were determined through the Shapiro-Wilk W-test. RESULTS : Of the 880 segments, 574 segments had relatively low COV and were classified as type 1 segments, and 123 and 64 segments with increasing and decreasing traffic volume trends were classified as type 2 and type 3 segments, respectively; 80 segments that failed the normality test were classified as type 4, and the remaining 39 were classified as type 5 segments. CONCLUSIONS : A theoretical basis for biennial count planning was established. Biennial count is recommended for types 1~4 because their mean absolute percentage errors (MAPEs) are approximately 10%. For type 5 (MAPE =19.26%), the conventional annual count can be continued. The results of this analysis can reduce the traffic monitoring budget.

제한되어 있는 자원으로 인해 최근 신재생에너지에 대한 관심이 고조되고 있다. 신재생에너지 중에서 도 특히 태양광 발전이 단순한 구조로 이루어져 있고, 위험성이 적어 타 재생에너지 시장보다 빠르게 성 장하고 있다. 따라서 태양광의 발전 효율을 성장시키는 많은 연구가 진행되어 왔다. 태양광 발전은 태양 광 패널의 온도나 패널 표면의 먼지, 눈 등의 오염원에 따라 발전 효율에 영향을 미치지만, 태양전지를 개 발하거나 보완하는 연구가 대부분 이루어지고 있으며 기 개발된 패널의 발전 효율을 높이는 연구는 미비 한 실정이다. 하여 본 연구는 패널 표면의 오염원을 자동으로 청소해주는 클리닝 시스템을 개발하여 적용 유무에 따른 발전 효율을 비교 분석하였다. 2016년 1월 강설 시 테스트를 수행하여 발전 효율 분석한 결 과, 5분 단위로 최대 3.3% 증가하였고, 1시간 동안 약 1% 효율이 증가하는 것으로 분석되었다. 패널 온도 는 클리닝 시스템 적용 유무에 관계없이 유사하게 나타났다. 발전 효율이 미미한 수치로 증가한 것은 적 설량이 적었기 때문인 것으로 유추된다. 향후에 많은 량의 강설 발생 시, 클리닝 시스템을 적용한다면 발 전 효율이 더 증가할 것으로 판단된다. 또한, 이러한 결과에 따라 태양광 발전 시스템 시장에서도 필요한 요소로 성장할 수 있을 것으로 기대된다.

본 연구에서는 평택 미8군 차량정비시설 내 콘크리트 포장의 줄눈부 하중전달효율(Load Transfer Efficiency, LTE)을 평가하기 위해 FWD(Falling Weight Deflectometer)를 이용한 조사･평가를 수행하 였다. 본 연구에서는 <그림 1>에서와 같이 FHWA/TX-07/0-5123-2“Guidelines for Evaluation of Existing Pavement for HMA Overlay”에서 제시한 FWD 처짐 하중전달효율 산정식을 사용하였다. FWD를 이용한 콘크리트 슬래브 줄눈부의 하중전달효율을 조사한 결과는 <표 1>에서 보는 바와 같다. 하중전달효율이 80% 이상인 지점은 97개소로 전체의 약 84.3%에 해당한다. 콘크리트 슬래브 시공 당시 줄눈부에 <그림 2>와 같은 배부름현상(Bulging)이 발생하였다. 배부름현상 이 하중전달효율에 미치는 영향을 파악하기 위하여 총 24개 줄눈부에 대하여 순방향과 역방향으로 <그림 2>와 같이 FWD 시험을 수행하였다. <그림 3>은 각 줄눈부에서 Case 1과 Case 2의 하중전달효율을 비교 한 것이다. 그래프에서 보는 바와 같이 LTE 차이가 ±2% 이내인 경우를 (6개 지점) 제외하고 총 18개 줄 눈부 중 15개 줄눈부에서 Case 1이 Case 2보다 LTE가 큰 것으로 나타나 줄눈부 배부름현상이 LTE에 영 향을 주는 것으로 나타났다.

고분자막이 가진 수투과능 한계와 비가역적 막오염에 의한 효율 저하는 막기반 수처리공정의 추가적인 경쟁력 확보를 위해 해결해야 하는 문제이다. 본 연구에서는 초고속 물투과 특성과 뛰어난 선택성을 지닌 Graphene oxide (GO)막 에 뛰어난 항균성을 가진 은나노(nAg)가 코팅된 막을 제작, 평가하였다. GO막 은 막오염 저감에 효과적일 수 있는 높은 친수성과 음표면전하와 같은 특성과 막오염에 취약할 수 있는 높은 비표면적과 뛰어난 흡착능력과 같은 특성을 동 시에 가진다. 따라서, 논란의 부분이 있는 GO막의 막오염에 대한 저항성의 확실한 향상을 위해 nAg를 적용하였다.

이 논문은 비모수적 자료포락분석 기법을 이용하여 감귤농가의 경영효율성을 추정하였다. 추가적으로 커널밀도함수를 이용하여 경영효율성의 분포가 지난 13년간 어떻게 바뀌었는지도 도식화하였다. 연구를 위해 농촌진흥청에서 조사 발표하는 자료를 사용하였다. 분석결과를 보면, 지난 13년간 경영효율성은 시설 감귤농가와 노지 감귤농가 모두에서 상승하는 것으로 나타났다. 이것은 수입농산물이 증가하는 상 황 하에서 감귤농가의 경영효율성이 개선되고 있음을 의미한다. 경영효율성을 순수기술적 효율성과 규 모 효율성으로 분해한 결과, 순수기술적 효율성은 점차 개선되는 반면에 규모 효율성은 악화되는 것으 로 분석되었다. 하지만 여전히 효율성 개선의 여지는 순수기술적 효율성 측면에 있는 것으로 분석되었 다. 순수기술적 효율성은 꾸준히 계속 향상시키되 적정규모를 찾아가는 노력도 병행해야 할 것으로 보 인다.

The purpose of this study is to investigate production efficiency of Oceans and Fisheries Human Resources Development Programs Efficiency using Bootstrap-DEA. The study extracts 33 officials curriculum, 11 fisheries managers curriculum for its analytical. First, the study estimates technical, pure technical, and scale efficiency of each curriculums based on traditional DEA under the assumption of CRS and VRS. 8(official 7, managers 1) curriculums are identified as efficient DMUs under the CCR-model, and 13(official 10, managers 3) under the BCC-model. We provide inputs that allow inefficient curriculum to be efficient DMUs on a production frontier, and a reference set for their bench-marking. Second, rank test, Wilcoxon-Mann-Whitney test to find a statistical significance of heterogeneity existing in efficiences between Bootstrap-DEA tenical vs Bootstrap-DEA pure technical was no significant difference. We have identified that G10, 11, 12 13, 25, 31, 33, 39 curriculums are the most efficiently produced in the technical and pure technical efficiency. Also we managed to measure the inefficiency which exists in efficiently produced curriculums when estimating the bias corrected efficiency scores. In Technical efficiency, Operation and facility was significant at the 10%. In Pure technical efficiency, facility was significant at the 10%.

The blast hole has a space between explosive and hole wall, and blast forces reaches the hole wall face with a large amount of loss during passing through this space. The loss ratio of blasting forces are different to this packing material of space like air, water, etc. In this study, the effect of packing materials is investigated by the numerical simulation analysis. The simulation is carried out to two phases; 1st phase is to compute the impacting forces reaching on the wall face passing through packing materials(air, water), 2nd phase is to study the blasting effect(block size of cracking, direction of driving forces, etc.) in real site. The reaching force at the wall face in the water is larger than in the air. This study shows that the water as packing materials is superior to the air.

Infrastructure such as tracks, catenary, rolling stock and signaling systems, and close interface with the human factors of train drivers, shunting staff and maintenance staff are required for train operations. Safety management is the most essential part for the functioning of a train. In line with the safety management, each rail operator has a safety guidance system and the Ministry of Land, Infrastructure and Transport operates Railway Safety Inspector system. Safety Inspector System is to train employees working in the field to be aware of rules and regulations, and to improve identified risk factors. Hence, it is necessary to establish systematic training and inspection methods for safety guidance staff and make clear the roles and responsibilities of safety inspectors according to the Occupational Safety and Health Act, and safety instructors for several inspections according to the Railroad Safety Act.

The purpose of this study is to assess the effects of outlook project for olive flounder, from the view point of price stabilization, market efficiency, and causality of the prices in different distribution channels. Analytical results show that the volatility of producer price of olive flounder has been significantly mitigated after the implementation of the outlook project. The market efficiency is estimated to be improved after implementing the outlook project although there is an inefficiency on price determination process in some producing regions. The causality test on the producer and wholesale price shows that producing stage leads the wholesale stage in forming the prices. It is found that Jeju leads the flounder price on the size of 500g and 2kg, while Wando leads the price of 1kg size. These estimation result as whole indicate that outlook project for olive flounder has accomplished the intended goals.

PURPOSES: The objective of this study is to determine the milling temperature that minimizes the binder-induced damage to the aggregate; this is achieved by evaluating the temperature dependence of the viscosity of the asphalt binder, with the aim of developing an effective heating process for warm in-place recycling. METHODS : The validity of the indoor test was confirmed by conducting an internal heating test based on the on-site heating test. In addition, the adhesive power of the binder was measured at various temperatures (30℃, 40℃, 50℃, 60℃, 70℃) via three types of measuring methods. RESULTS: The surface temperature spectrum of field test was slight different with that of laboratory test. But, the spectra of inner temperature between the field and the laboratory was almost similar. Also, the adhesion of the asphalt binder was measured from 30℃ to 70℃. The adhesion of the binder was significantly decreased from 60℃. Contrary to other temperature, the adhesion was slightly changed from 60℃ to 70℃. Also the inner temperature between two different heating methods was shown similar temperature spectra. CONCLUSIONS: The pavement heating temperature spectrum of hot in place recycling method was simulated by a laboratory test. Based on this study, the optimum temperature was 60℃~70℃ for reducing aggregate damage during milling process. The susceptibility heating method developed in this study can be maintained the optimum inner temperature range.