본 연구는 스포츠 휴먼브랜드의 굿즈상품(Goods) 가치, 감정반응(PAD), 소비 행동 간 일어나는 현상을 분석하기 위해 2021년 10월 18일~2022년 1월 12일까지 온라인 서베이(URL), DM, E-mail 등을 활용하여 371명을 편의 표준추출법으로 표집하였으며, 불성실한 응답자 51명을 제외한 유효 표본 320명을 인과 관계(SEM)를 적용하여 분석하였다. 첫째, 휴먼 브랜드의 굿즈 가치는 감정반응에 정(+)의 영향으로 가설이 채택되었다. 둘째, 감정반응은 소비 행동에 정(+)의 영향을 미치는 것으로 가설이 채택되었다. 셋 째, 휴먼 브랜드의 굿즈 가치는 소비 행동에 정(+)의 영향을 미치며 부분적으로 가설이 채택되었다. 마지막 으로 굿즈상품(goods) 가치와 소비 행동의 관계에서 감정반응의 간접효과는 통계적으로 유의 의미한 것으 로 나타났다.

The increasing prevalence of obesity is associated with various metabolic diseases such as diabetes, posing significant social and economic burdens to the society. While obesity is a complex disease with multiple factors contributing to its pathophysiology, altered glucose and lipid metabolism is evident in obese patients as well as in animal models. Abnormal metabolic regulation often leads to development of insulin resistance, a hallmark of obesity-induced type 2 diabetes. In this review, we provide a brief overview of altered lipid metabolism manifested in the obese state. In addition, two representative animal models, Mus musculus and Drosophila melanogaster, are presented with experimental approaches adopted for generation and utilization of these models. More specifically, Drosophila has been widely used for studying the core physiological phenomena across phyla for decades, mostly due to the ease of handling and sophisticated genetic manipulations with conserved cell signaling pathways of reduced redundancy. Considering a significant degree of homology in Drosophila for human disease-associated genes, it poses as a versatile in vivo platform to study the pathophysiology of various human diseases. With core metabolic pathways governing energy homeostasis generally conserved, Drosophila can be used as a model for studying molecular mechanisms underlying disease phenotypes manifested in obese and diabetic patients. In this review, we discuss representative Drosophila studies that investigated the effects of dysregulated core signaling pathways on metabolic signatures of obese animals.

Mesenchymal stem cells (MSCs) have been recognized as a therapeutic tool for various diseases due to its unique ability for tissue regeneration and immune regulation. However, poor survival during in vitro expansion and after being administrated in vivo limits its clinical uses. Accordingly, protocols for enhancing cell survivability is critical for establishing an efficient cell therapy is needed. CDDOMe is a synthetic C-28 methyl ester of 2-cyano-3,12-dioxoolean-1,9-dien-28-oic acid, which is known to stimulate nuclear factor erythroid 2-related factor 2 (Nrf2)- antioxidant response element (ARE) pathway. Herein, report that CDDO-Me promoted the proliferation of MSCs and increased colony forming units (CFU) numbers. No alteration in differentiation into tri-lineage mesodermal cells was found after CDDO-Me treatment. We observed that CDDO-Me treatment reduced the cell death induced by oxidative stress, demonstrated by the augment in the expression of Nrf2-downstream genes. Lastly, CDDO-Me led to the nuclear translocation of NRF2. Our data indicate that CDDO-Me can enhance the functionality of MSCs by stimulating cell survival and increasing viability under oxidative stress.

Mutations in the luteinizing hormone/chorionic gonadotropin receptors (LH/CGRs), representatives of the G protein-coupled receptor family, have been rapidly identified over the last 20 years. This review aims to compare and analyze the data reported the activating and inactivating mutations of the LH/CGRs between human, rat, equine and fish, specifically (Japanese eel Anguilla japonica). Insights obtained through detailed study of these naturally-occurring mutations provide a further update of structure-function relationship of these receptors. Specifically, we present a variety of data on eel LH/CGR. These results provide important information about LH/CGR function in fish and the regulation of mutations of the highly conserved amino acids in glycoprotein hormone receptors.

This study examined the previous studies and reviews on the impact of human animal bond (HAB), a coevolutionary relationship between human and animal, in trauma recovery. The most important factor in recovering from trauma is making a good relationship; human animal bond can act as a positive aspect in making such relationship. Animals give a sense of support and safety which leads to making a trustful relationship. It has also been proven HAB can lessen one's loneliness and anxiety. For these reasons, Animal Assisted Therapy (AAT) is widely used in trauma recoveries.

본 연구에서는 인체에 보다 안전한 천연 화장품보존제 발굴을 목표로 하여 인간유래 항균펩타 이드 LL-37의 짧은 유사체인 FK-13을 화장품보존제로 사용하고자 연구를 진행하였다. 이를 위해 13개의 아미노산으로 구성된 FK-13 펩타이드를 고상 펩타이드 합성법로 합성하였고 reversed phase-high performance liquid chromatography (RP-HPLC)로 정제 후 liquid chromatography-mass spectrometry (LC-MS)를 이용하여 순도와 분자량을 확인하였다. 합성된 FK-13을 이용하여 미생물에 대한 방부력을 검 정하였고 화장품에 적용 가능성을 살펴보았다. FK-13은 3개의 그람-양성균 (Staphylococcus aureus, Bacillus subtilis, Staphylococcus epidermidis)과 3개의 그람-음성균 (Escherichia coli, Salmonella typhimurium, Pseudomonas aeruginosa), 그리고 진균인 Candida glabrata 에서도 높은 항균 활성을 보였 으며 넓은 항균활성 스펙트럼을 나타내었다. 이를 바탕으로 화장품보존제로서의 적합성을 확인하였다. 또 한 FK-13은 고온에서 분자의 열안정성을 보였으며 기존의 천연 한방화장품 방부제 및 화학보존제들과의 항균활성 비교 실험에서도 보다 월등한 항균활성 효능을 나타내었다. 따라서 FK-13 펩타이드는 인체 친화 적이고 효과적인 항균활성을 나타내므로, 기존 화학보존제를 대체할 새로운 천연 화장품보존제로 적용이 가능할 것으로 판단된다.

본 연구는 골프 광고 모델의 휴먼브랜드 영역이 확장되면서 일어나는 현상에 대해 분석하고자 2021년 7월 1일 ~11월 15일까지 표본계획에 의해 모바일 서베이(URL)를 사용하여 521명을 인과 관계 (SEM)를 적용 분석하였다. 첫째, 골프 광고 모델의 휴먼브랜드는 적합성에 통계적으로 유의미한 정(+)의 영향으로 가설이 채택되었다. 둘째, 적합성은 구매행도에 통계적으로 유의미한 정(+)의 영향으로 가설이 채택되었다. 셋째, 골프 광고 모델의 휴먼브랜드는 구매행동에 통계적으로 유의미한 정(+)의 영향으로 가 설이 채택되었다. 마지막으로 부트스트레핑(bootstrapping)을 통해 간접효과를 산출한 결과 휴먼브랜드와 구매행동의 관계에서 적합성의 간접효과는 통계적으로 유의하다고 볼 수 있다.

본 연구는 미역(Undaria pinnatifida) 추출물과 쇠미역(Costaria costata) 추출물의 항산화 활성 및 HepG2 세포의 알코올로 인한 산화 손상에 대한 보호 효과를 연구하였다. 미역과 쇠미역 추 출물의 총 폴리페놀과 플라보노이드 함량은 70% 에탄올 추출물에서 가장 높았다. 또한 미역과 쇠미역 70% 에탄올 추출물의 DPPH (IC50 0.33±0.21, 0.48±0.47 mg/ml), ABTS (IC50 0.34±0.30, 0.47±0.17 mg/ml) 라디칼 소거 활성이 열수 추출물 및 10% 에탄올 추출물 보다 높았다. 추출 물의 간 보호 효과를 확인하기 위하여 HepG2 세포에 알코올 산화 스트레스를 유발하여 MTT 분석을 이용하여 세포 생존력을 측정하였다. 미역 및 쇠미역 열수 추출물은 알코올 처리군 (73.95%) 대비 세포 생존율은 각각 89.91~97.63% 및 84.99~90.54%로 농도 의존적으로 증가시 켰다. 본 연구는 미역과 쇠미역 추출물이 간 보호 및 항산화 효과를 나타냄을 확인하였고 알코 올 산화 손상에 대한 간 보호 소재로 활용될 수 있을 것으로 사료된다.

Despite evidence that bacteria-sensing Toll-like receptors (TLRs) are activated in salivary gland tissues of Sjogren syndrome (SS) patients, the role of oral bacteria in SS etiopathogenesis is unclear. We previously reported that two SS-associated oral bacteria, Prevotella melaninogenica (Pm) and Rothia mucilagenosa (Rm), oppositely regulate the expression of major histocompatibility complex class I (MHC I) in human salivary gland (HSG) cells. Here, we elucidated the mechanisms underlying the differential regulation of MHC I expression by these bacteria. The ability of Pm and Rm to activate TLR2, TLR4, and TLR9 was examined using TLR reporter cells. HSG cells were stimulated by the TLR ligands, Pm, and Rm. The levels of MHC I expression, bacterial invasion, and viability of HSG cells were examined by flow cytometry. The hypoxic status of HSG cells was examined using Hypoxia Green. HSG cells upregulated MHC I expression in response to TLR2, TLR4, and TLR9 activation. Both Pm and Rm activated TLR2 and TLR9 but not TLR4. Rm-induced downregulation of MHC I strongly correlated with bacterial invasion and cell death. Rm-induced cell death was not rescued by inhibitors of the diverse cell death pathways but was associated with hypoxia. In conclusion, Pm upregulated MHC I likely through TLR2 and TLR9 activation, while Rm-induced hypoxia-associated cell death and the downregulation of MHC I, despite its ability to activate TLR2 and TLR9. These findings may provide new insight into how oral dysbiosis can contribute to salivary gland tissue damage in SS.

Ms. Hee Choon Oh was a Jeju Haenyeo(woman diver) and one of the few surviving victims of the Jeju 4.3 (1948). As a Haenyeo: Jeju society was very poor at the time during the Japanese colonial period and the division of the two Korea, especially after the Korean War, so she had to work regardless of gender. It was hard to go into the sea, but it was the only thing to support my family. In retrospect, Going out to work in the sea was like a life-threatening adventure every day. While there were many emergency moments, She becomes one with the sea and forgot all the pain. After having spent a lot of time together with the sea, I had to stop working as a Haenyeo because it was hard to take care of nine children after having heart surgery. I was able to receive Honorary Haenyeo through continuous exchanges and dedication to the society. As a victim of the Jeju 4.3: I had no choice but to explain it why I had to do work as a Haenyeo and to understand my life. I am a woman who chose the job of Haenyeo, but I have lived through the pain of the dark modern and contemporary history of Korea. The unfair one-year prison life that I wanted to hide even from my children hurt me all my life. Over the past 70 years, sharing pain with the sea, neighbors, and family, serving the region, and hopefully waiting for a better society and justice to come. and finally It was not until 2019, 70 years after 1948, that I was officially acquitted by a Korean court. I regained my honor as a Haenyeo and was able to get rid of my deep sadness.

Nypa fruticans Wurmb (NFW) contains a large amount of phenolic acid and flavonoids, and is popular as a superfood in Myanmar. NFW has various biological activities, such as anti-inflammatory, anti-oxidant, and neuroprotective properties; however, the anti-cancer effect of NFW have not been reported. In this study, we investigated the anticancer activity of water extracts of NFW (WeNFW) and the underlying mechanism in human FaDu hypopharyngeal squamous carcinoma cells. The WeNFW inhibited FaDu cell growth in a dose-dependent manner without affecting normal cells (L929), as determined by an MTT assay and Live and Dead assay. In addition, the concentrations of WeNFW without cytotoxicity (0.025, 0.05, and 0.1 mg/mL) inhibited wound healing and colony formation. Furthermore, WeNFW significantly induced apoptosis through the proteolytic cleavage of caspase-3 and -9, poly (ADP-ribose) polymerase, and downregulation of Bcl-2 and upregulation of Bax in FaDu cells, as determined by DAPI staining, FACS analysis, and western blot analysis. Taken together, these results suggest that WeNFW exhibits potent anti-cancer effects by suppressing the growth of oral cancer cells, wound healing and colony formation activity. Via mitrochondrial-dependent apoptotic pathways in human FaDu hypopharyngeal squamous carcinoma cells. Therefore, WeNFW can provide a natural chemotherapeutic drug for oral cancer in humans.

본 연구는 대규모 온실에서 작업관리와 인력관리 및 병해충 모니터링을 위한 HRM 프로그램을 개발하여 현장적용 시험을 수행하였다. 작업자의 전용 앱으로 작업지시와 현장의 작업내용 및 병해충 발생에 대한 정보를 입력할 수 있다. 관리자 는 작업자의 작업내용별 노동시간과 작업속도, 작업품질을 평가하여 기록하고 이를 토대로 작업별 작업속도 기준을 설정할 수 있으며, 작업일정 수립이나 작업 투입인원의 결정이 용이하게 될 것이다. 따라서 고용인원을 효율적으로 운용하면서 작업수행을 용이하게 할 수 있으며, 작업자의 수급계획을 세울 때도 용이하게 사용될 수 있다. 시기별 작업분석을 통해 고용인원의 적정성을 평가할 수 있고 정규인력과 비정규 인력에 대한 수급비율을 조정할 수 있게 할 것이다. 작업자의 실시간 작업성과를 공개하며 작업자 간 경쟁을 유도하고 기준작업량 이하를 달성하는 작업자의 재교육 및 작업방법 개선을 통해 작업효율의 상승이 가능하다. 실증현장에서 분석해본 파프리카의 작업은 순치기, 수확 및 유인작업이 모든 작업시간의 91%이상을 차지하며, 수확작업보다 유인 및 순치기 작업 시간이 두배정도 더 많은 것으로 나타났다. HRM 프로그램을 도입하기 전과 도입한 이후 가장 노동부하가 높은 시기인 수확과 유인 및 순치기 작업이 동시에 진행되는 시기를 비교했을 때 HRM 프로그램의 도입으로 37%의 작업량 향상을 가져 왔다.